################################## # NO CUT ENZYMES ( 113 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AgeI AhdI AocI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbrPI BbsI BbvCI BcgI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaI BsaWI BseAI BsiWI BsmI BspDI BspEI BspHI BsrDI BssHII Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DraI Eam1105I EarI Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FspI HindIII HpaI KpnI Ksp632I MaeI MaeIII MboII MfeI MluI MroI MseI MunI NdeI NheI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SapI SbfI ScaI SexAI SfuI SmlI SnaBI SpeI SphI Sse8387I SspI SwaI TfiI Tru9I Tsp45I Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AccI 1060 GTCTAC GT^[AC][GT]AC AflIII 1316 ACATGT A^C[GA][CT]GT Alw44I 1320 GTGCAC G^TGCAC Aor51HI 1346 AGCGCT AGC^GCT ApaI 586 GGGCCC GGGCC^C ApaLI 1320 GTGCAC G^TGCAC BbeI 748 GGCGCC GGCGC^C BciVI 985 GGATAC GTATCC(6/5) BsaBI 855 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmBI 580 GAGACG CGTCTC(1/5) BspLU11I 1316 ACATGT A^CATGT BspMI 960 ACCTGC ACCTGC(4/8) BstAPI 66 GCAGCGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 1511 CCACCATCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraIII 331 CACATCGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1228 GACAGCCGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 1346 AGCGCT AGC^GCT FseI 546 GGCCGGCC GGCCGG^CC HincII 1546 GTTGAC GT[CT]^[GA]AC HindII 1546 GTTGAC GT[CT]^[GA]AC HinfI 385 GACTC G^A[ACGT]TC KasI 748 GGCGCC G^GCGCC MamI 855 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NarI 748 GGCGCC GG^CGCC NotI 1717 GCGGCCGC GC^GGCCGC NspI 1316 ACATGT [GA]CATG^[CT] PflMI 1617 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 385 GACTC GAGTC(4/5) SacI 369 GAGCTC GAGCT^C SfiI 117 GGCCTTCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 748 GGCGCC GGC^GCC Tsp509I 1253 AATT ^AATT Van91I 1617 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 566 CCAAGCGCCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AspHI 369 GAGCTC G[AT]GC[AT]^C 1320 GTGCAC BsgI 13 CTGCAC GTGCAG(16/14) 1100 GTGCAG BsiHKAI 369 GAGCTC G[AT]GC[AT]^C 1320 GTGCAC Bsp1407I 302 TGTACA T^GTACA 857 TGTACA BsrGI 302 TGTACA T^GTACA 857 TGTACA BssSI 292 CTCGTG CACGAG(-5/-1) 949 CTCGTG EcoT14I 530 CCATGG C^C[AT][AT]GG 830 CCATGG MaeII 476 ACGT A^CGT 1301 ACGT NcoI 530 CCATGG C^CATGG 830 CCATGG PstI 319 CTGCAG CTGCA^G 445 CTGCAG SfcI 319 CTGCAG C^T[GA][CT]AG 445 CTGCAG SgrAI 1581 CGCCGGCG C[GA]^CCGG[CT]G 1712 CGCCGGCG SmaI 590 CCCGGG CCC^GGG 1024 CCCGGG SspBI 302 TGTACA T^GTACA 857 TGTACA StyI 530 CCATGG C^C[AT][AT]GG 830 CCATGG XmaCI 590 CCCGGG C^CCGGG 1024 CCCGGG XmaI 590 CCCGGG C^CCGGG 1024 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 94 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI AvaI AvaII BalI BanI BanII BbvI BcnI BglI BmyI BpmI BsaHI BsaJI BseRI BsiEI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrBI BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EagI EclXI Eco52I EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII HphI ItaI KspI MboI MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV PpuMI PvuII RsaI SacII Sau3AI Sau96I ScrFI SfaNI StuI TaqI TseI TspRI TthHB8I XhoII