(Linear) (Six-base) MAPSORT of: KIEE3941.seq Check: 7969 from: 1 to: 1761 KIEE3941.orf With 182 enzymes: SgfI * March 22, 2012 16:55 .. AccI GT'mk_AC Cuts at: 0 1061 1761 Size: 1061 700 AfeI AGC'GCT Cuts at: 0 1348 1761 Size: 1348 413 AflIII A'CryG_T Cuts at: 0 1316 1761 Size: 1316 445 AleI CACnn'nnGTG Cuts at: 0 832 1097 1516 1761 Size: 832 265 419 245 Fragments arranged by size: 832 419 265 245 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 460 1761 Size: 460 1301 AlwNI CAG_nnn'CTG Cuts at: 0 12 72 243 1054 1761 Size: 12 60 171 811 707 Fragments arranged by size: 811 707 171 60 12 ApaI G_GGCC'C Cuts at: 0 590 1761 Size: 590 1171 ApaLI G'TGCA_C Cuts at: 0 1320 1761 Size: 1320 441 AvaI C'yCGr_G Cuts at: 0 512 590 1024 1441 1633 1761 Size: 512 78 434 417 192 128 Fragments arranged by size: 512 434 417 192 128 78 BanI G'GyrC_C Cuts at: 0 346 492 675 748 1741 1761 Size: 346 146 183 73 993 20 Fragments arranged by size: 993 346 183 146 73 20 BanII G_rGCy'C Cuts at: 0 373 425 433 590 1414 1663 1761 Size: 373 52 8 157 824 249 98 Fragments arranged by size: 824 373 249 157 98 52 8 BbeI G_GCGC'C Cuts at: 0 752 1761 Size: 752 1009 BciVI GTATCCnnnnn_n' Cuts at: 0 979 1761 Size: 979 782 BglI GCCn_nnn'nGGC Cuts at: 0 77 124 133 466 616 1761 Size: 77 47 9 333 150 1145 Fragments arranged by size: 1145 333 150 77 47 9 Bme1580I G_kGCm'C Cuts at: 0 497 590 637 680 1324 1433 1744 1761 Size: 497 93 47 43 644 109 311 17 Fragments arranged by size: 644 497 311 109 93 47 43 17 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 939 1761 Size: 939 822 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 598 934 1057 1761 Size: 598 336 123 704 Fragments arranged by size: 704 598 336 123 Bpu10I CC'TnA_GC Cuts at: 0 899 1761 Size: 899 862 BsaBI GATnn'nnATC Cuts at: 0 859 1761 Size: 859 902 BsaHI Gr'CG_yC Cuts at: 0 410 749 1505 1535 1580 1711 1761 Size: 410 339 756 30 45 131 50 Fragments arranged by size: 756 410 339 131 50 45 30 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 400 430 829 859 1761 Size: 400 30 399 30 902 Fragments arranged by size: 902 400 399 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 811 820 853 1603 1761 Size: 811 9 33 750 158 Fragments arranged by size: 811 750 158 33 9 BseYI C'CCAG_C Cuts at: 0 48 75 679 1210 1276 1696 1761 Size: 48 27 604 531 66 420 65 Fragments arranged by size: 604 531 420 66 65 48 27 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1121 1761 Size: 1121 640 BsiEI CG_ry'CG Cuts at: 0 311 455 548 1721 1761 Size: 311 144 93 1173 40 Fragments arranged by size: 1173 311 144 93 40 BsiHKAI G_wGCw'C Cuts at: 0 373 1324 1761 Size: 373 951 437 Fragments arranged by size: 951 437 373 BsmBI CGTCTCn'nnnn_ Cuts at: 0 574 1761 Size: 574 1187 Bsp1286I G_dGCh'C Cuts at: 0 373 425 433 497 590 637 680 1324 Size: 373 52 8 64 93 47 43 644 Cuts at: 1324 1414 1433 1663 1744 1761 Size: 90 19 230 81 17 Fragments arranged by size: 644 373 230 93 90 81 64 52 47 43 19 17 8 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 969 1761 Size: 969 792 BsrBI CCG'CTC Cuts at: 0 735 923 1193 1494 1761 Size: 735 188 270 301 267 Fragments arranged by size: 735 301 270 267 188 BsrFI r'CCGG_y Cuts at: 0 310 412 450 543 547 1582 1713 1761 Size: 310 102 38 93 4 1035 131 48 Fragments arranged by size: 1035 310 131 102 93 48 38 4 BsrGI T'GTAC_A Cuts at: 0 302 857 1761 Size: 302 555 904 Fragments arranged by size: 904 555 302 BssSI C'ACGA_G Cuts at: 0 292 949 1761 Size: 292 657 812 Fragments arranged by size: 812 657 292 BstAPI GCAn_nnn'nTGC Cuts at: 0 72 1761 Size: 72 1689 BstXI CCAn_nnnn'nTGG Cuts at: 0 1518 1761 Size: 1518 243 BstYI r'GATC_y Cuts at: 0 23 239 323 1761 Size: 23 216 84 1438 Fragments arranged by size: 1438 216 84 23 BtgI C'CryG_G Cuts at: 0 464 530 611 830 1310 1457 1721 1761 Size: 464 66 81 219 480 147 264 40 Fragments arranged by size: 480 464 264 219 147 81 66 40 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 104 1761 Size: 104 1657 BtsI GCAGTG_nn' Cuts at: 0 378 890 1761 Size: 378 512 871 Fragments arranged by size: 871 512 378 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1131 1166 1761 Size: 1131 35 595 Fragments arranged by size: 1131 595 35 DraIII CAC_nnn'GTG Cuts at: 0 336 1761 Size: 336 1425 DrdI GACnn_nn'nnGTC Cuts at: 0 1234 1761 Size: 1234 527 EaeI y'GGCC_r Cuts at: 0 143 278 308 452 545 608 686 849 Size: 143 135 30 144 93 63 78 163 Cuts at: 849 1074 1139 1166 1625 1653 1718 1761 Size: 225 65 27 459 28 65 43 Fragments arranged by size: 459 225 163 144 143 135 93 78 65 65 63 43 30 28 27 EagI C'GGCC_G Cuts at: 0 308 452 545 1718 1761 Size: 308 144 93 1173 43 Fragments arranged by size: 1173 308 144 93 43 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 928 1404 1761 Size: 928 476 357 Fragments arranged by size: 928 476 357 EcoICRI GAG'CTC Cuts at: 0 371 1761 Size: 371 1390 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 598 934 1057 1761 Size: 598 336 123 704 Fragments arranged by size: 704 598 336 123 EcoO109I rG'GnC_Cy Cuts at: 0 586 587 1020 1236 1703 1754 1761 Size: 586 1 433 216 467 51 7 Fragments arranged by size: 586 467 433 216 51 7 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 213 1761 Size: 213 1548 FseI GG_CCGG'CC Cuts at: 0 551 1761 Size: 551 1210 HaeII r_GCGC'y Cuts at: 0 72 197 573 752 1350 1761 Size: 72 125 376 179 598 411 Fragments arranged by size: 598 411 376 179 125 72 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 400 432 1761 Size: 400 32 1329 Fragments arranged by size: 1329 400 32 HincII GTy'rAC Cuts at: 0 1548 1761 Size: 1548 213 KasI G'GCGC_C Cuts at: 0 748 1761 Size: 748 1013 MscI TGG'CCA Cuts at: 0 688 1141 1168 1655 1761 Size: 688 453 27 487 106 Fragments arranged by size: 688 487 453 106 27 MslI CAynn'nnrTG Cuts at: 0 832 1097 1405 1516 1761 Size: 832 265 308 111 245 Fragments arranged by size: 832 308 265 245 111 MspA1I CmG'CkG Cuts at: 0 179 397 444 466 504 576 613 912 Size: 179 218 47 22 38 72 37 299 Cuts at: 912 992 1080 1175 1224 1312 1459 1641 1723 Size: 80 88 95 49 88 147 182 82 Cuts at: 1723 1761 Size: 38 Fragments arranged by size: 299 218 182 179 147 95 88 88 82 80 72 49 47 38 38 37 22 NaeI GCC'GGC Cuts at: 0 312 414 452 545 549 1584 1715 1761 Size: 312 102 38 93 4 1035 131 46 Fragments arranged by size: 1035 312 131 102 93 46 38 4 NarI GG'CG_CC Cuts at: 0 749 1761 Size: 749 1012 NcoI C'CATG_G Cuts at: 0 530 830 1761 Size: 530 300 931 Fragments arranged by size: 931 530 300 NgoMIV G'CCGG_C Cuts at: 0 310 412 450 543 547 1582 1713 1761 Size: 310 102 38 93 4 1035 131 48 Fragments arranged by size: 1035 310 131 102 93 48 38 4 NotI GC'GGCC_GC Cuts at: 0 1718 1761 Size: 1718 43 NspI r_CATG'y Cuts at: 0 1320 1761 Size: 1320 441 PasI CC'CwG_GG Cuts at: 0 726 1761 Size: 726 1035 PciI A'CATG_T Cuts at: 0 1316 1761 Size: 1316 445 PflMI CCAn_nnn'nTGG Cuts at: 0 1623 1761 Size: 1623 138 PfoI T'CCnGG_A Cuts at: 0 1616 1761 Size: 1616 145 PpuMI rG'GwC_Cy Cuts at: 0 1020 1236 1754 1761 Size: 1020 216 518 7 Fragments arranged by size: 1020 518 216 7 PspOMI G'GGCC_C Cuts at: 0 586 1761 Size: 586 1175 PstI C_TGCA'G Cuts at: 0 323 449 1761 Size: 323 126 1312 Fragments arranged by size: 1312 323 126 PvuII CAG'CTG Cuts at: 0 444 1080 1175 1761 Size: 444 636 95 586 Fragments arranged by size: 636 586 444 95 SacI G_AGCT'C Cuts at: 0 373 1761 Size: 373 1388 SacII CC_GC'GG Cuts at: 0 467 614 1313 1460 1724 1761 Size: 467 147 699 147 264 37 Fragments arranged by size: 699 467 264 147 147 37 SanDI GG'GwC_CC Cuts at: 0 1020 1761 Size: 1020 741 SfcI C'TryA_G Cuts at: 0 319 445 1761 Size: 319 126 1316 Fragments arranged by size: 1316 319 126 SfiI GGCCn_nnn'nGGCC Cuts at: 0 124 1761 Size: 124 1637 SfoI GGC'GCC Cuts at: 0 750 1761 Size: 750 1011 SgrAI Cr'CCGG_yG Cuts at: 0 1582 1713 1761 Size: 1582 131 48 Fragments arranged by size: 1582 131 48 SmaI CCC'GGG Cuts at: 0 592 1026 1761 Size: 592 434 735 Fragments arranged by size: 735 592 434 StuI AGG'CCT Cuts at: 0 118 557 820 868 1761 Size: 118 439 263 48 893 Fragments arranged by size: 893 439 263 118 48 StyI C'CwwG_G Cuts at: 0 530 830 1761 Size: 530 300 931 Fragments arranged by size: 931 530 300 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1599 1761 Size: 1599 162 TatI w'GTAC_w Cuts at: 0 302 857 1256 1761 Size: 302 555 399 505 Fragments arranged by size: 555 505 399 302 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 573 1761 Size: 573 1188 XmaI C'CCGG_G Cuts at: 0 590 1024 1761 Size: 590 434 737 Fragments arranged by size: 737 590 434 Enzymes that do cut: AccI AfeI AflIII AleI AlfI AlwNI ApaI ApaLI AvaI BanI BanII BbeI BciVI BglI Bme1580I BplI BpmI Bpu10I BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI BsrGI BssSI BstAPI BstXI BstYI BtgI BtgZI BtsI CspCI DraIII DrdI EaeI EagI EciI EcoICRI Eco57MI EcoO109I FalI FseI HaeII Hin4I HincII KasI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NotI NspI PasI PciI PflMI PfoI PpuMI PspOMI PstI PvuII SacI SacII SanDI SfcI SfiI SfoI SgrAI SmaI StuI StyI TaqII TatI XcmI XmaI Enzymes that do not cut: AarI AatII Acc65I AclI AcuI AflII AgeI AhdI AloI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI BclI BfrBI BglII BlpI BmgBI BmrI BmtI BpuEI BsaI BsaAI BsaWI BsiWI BsmI BspEI BspHI BsrDI BssHII BstBI BstEII BstZ17I Bsu36I ClaI DraI EarI EcoNI EcoRI EcoRV FspI FspAI HindIII HpaI KpnI MfeI MluI MmeI NdeI NheI NruI NsiI PacI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SalI SapI SbfI ScaI SexAI SgfI SmlI SnaBI SpeI SphI SrfI SspI SwaI TstI Tth111I XbaI XhoI XmnI ZraI