(Circular) (Six-base) MAPSORT of: pFN21AB0922.seq Check: 4086 from: 1 to: 5740 pFN21AB0922 5740 bp With 182 enzymes: SgfI * April 15, 2010 09:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3550 278 Size: 53 83 186 936 2014 2468 Fragments arranged by size: 2468 2014 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3083 1937 Size: 1146 4594 Acc65I G'GTAC_C Cuts at: 2945 3061 2945 Size: 116 5624 AclI AA'CG_TT Cuts at: 1563 3867 4240 1563 Size: 2304 373 3063 Fragments arranged by size: 3063 2304 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3016 3803 5187 5455 1632 Size: 1384 787 1384 268 1917 Fragments arranged by size: 1917 1384 1384 787 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5543 AflIII A'CryG_T Cuts at: 1203 4639 5616 1203 Size: 3436 977 1327 Fragments arranged by size: 3436 1327 977 AgeI A'CCGG_T Cuts at: 4571 4571 Size: 5740 AhdI GACnn_n'nnGTC Cuts at: 4469 4469 Size: 5740 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5740 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2552 2975 1184 Size: 1368 423 3949 Fragments arranged by size: 3949 1368 423 AlwNI CAG_nnn'CTG Cuts at: 2557 2813 3483 5055 2557 Size: 256 670 1572 3242 Fragments arranged by size: 3242 1572 670 256 ApaI G_GGCC'C Cuts at: 2245 2259 2907 2245 Size: 14 648 5078 Fragments arranged by size: 5078 648 14 ApaLI G'TGCA_C Cuts at: 2150 3797 4953 2150 Size: 1647 1156 2937 Fragments arranged by size: 2937 1647 1156 ApoI r'AATT_y Cuts at: 1493 2066 3049 3275 3427 5536 1493 Size: 573 983 226 152 2109 1697 Fragments arranged by size: 2109 1697 983 573 226 152 AseI AT'TA_AT Cuts at: 160 1032 4294 160 Size: 872 3262 1606 Fragments arranged by size: 3262 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5740 AvaI C'yCGr_G Cuts at: 1870 1943 2161 2648 3065 1870 Size: 73 218 487 417 4545 Fragments arranged by size: 4545 487 417 218 73 BamHI G'GATC_C Cuts at: 3070 3437 3070 Size: 367 5373 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2368 2945 3061 4516 618 Size: 325 217 627 581 577 116 1455 1842 Fragments arranged by size: 1842 1455 627 581 577 325 217 116 BanII G_rGCy'C Cuts at: 1391 2245 2259 2352 2907 3059 1391 Size: 854 14 93 555 152 4072 Fragments arranged by size: 4072 854 555 152 93 14 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2684 928 Size: 971 785 3984 Fragments arranged by size: 3984 971 785 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3931 3965 1903 Size: 34 1994 34 3678 Fragments arranged by size: 3678 1994 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3632 4842 3632 Size: 1210 4530 BclI T'GATC_A Cuts at: 1548 1548 Size: 5740 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2508 4351 136 Size: 107 122 71 631 605 836 1843 1525 Fragments arranged by size: 1843 1525 836 631 605 122 107 71 BglII A'GATC_T Cuts at: 5735 5735 Size: 5740 BlpI GC'TnA_GC Cuts at: 2823 3157 2823 Size: 334 5406 Bme1580I G_kGCm'C Cuts at: 1790 2154 2245 2259 2907 3801 4957 1790 Size: 364 91 14 648 894 1156 2573 Fragments arranged by size: 2573 1156 894 648 364 91 14 BmgBI CAC'GTC Cuts at: 1318 2173 2443 1318 Size: 855 270 4615 Fragments arranged by size: 4615 855 270 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2874 3471 4429 Size: 416 53 83 399 24 1457 597 958 Cuts at: 4429 442 Size: 1753 Fragments arranged by size: 1753 1457 958 597 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5740 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2284 2579 4400 1239 Size: 1045 295 1821 2579 Fragments arranged by size: 2579 1821 1045 295 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3865 4730 5028 3865 Size: 865 298 4577 Fragments arranged by size: 4577 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2453 4403 882 Size: 617 954 1950 2219 Fragments arranged by size: 2219 1950 954 617 BsaAI yAC'GTr Cuts at: 493 1204 2597 3531 493 Size: 711 1393 934 2702 Fragments arranged by size: 2702 1393 934 711 BsaBI GATnn'nnATC Cuts at: 5734 5734 Size: 5740 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2073 3547 3929 4564 Size: 53 83 186 936 540 1474 382 635 Cuts at: 4564 275 Size: 1451 Fragments arranged by size: 1474 1451 936 635 540 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4173 4571 4845 4992 5403 4173 Size: 398 274 147 411 4510 Fragments arranged by size: 4510 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2782 2812 1178 Size: 30 165 30 1379 30 4106 Fragments arranged by size: 4106 1379 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3030 1186 Size: 1844 3896 BseYI C'CCAG_C Cuts at: 2913 2997 4943 5439 2913 Size: 84 1946 496 3214 Fragments arranged by size: 3214 1946 496 84 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2219 2300 2465 2906 1163 Size: 573 483 81 165 441 3997 Fragments arranged by size: 3997 573 483 441 165 81 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2015 3190 3951 4979 1540 Size: 275 186 14 1175 761 1028 2301 Fragments arranged by size: 2301 1175 1028 761 275 186 14 BsiHKAI G_wGCw'C Cuts at: 2154 3801 3886 4957 2154 Size: 1647 85 1071 2937 Fragments arranged by size: 2937 1647 1071 85 BsmI GAATG_Cn' Cuts at: 3009 3251 3344 3009 Size: 242 93 5405 Fragments arranged by size: 5405 242 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2154 2245 2259 2352 2907 3059 3801 Size: 399 364 91 14 93 555 152 742 Cuts at: 3801 3886 4957 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 555 399 364 152 93 91 85 14 BspEI T'CCGG_A Cuts at: 5403 5403 Size: 5740 BspHI T'CATG_A Cuts at: 3629 5359 3629 Size: 1730 4010 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2214 2928 3095 844 Size: 1370 714 167 3489 Fragments arranged by size: 3489 1370 714 167 BsrBI CCG'CTC Cuts at: 1394 1823 2115 2563 3627 1394 Size: 429 292 448 1064 3507 Fragments arranged by size: 3507 1064 448 429 292 BsrDI GCAATG_nn' Cuts at: 65 1248 4235 4409 65 Size: 1183 2987 174 1396 Fragments arranged by size: 2987 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4384 4571 1700 Size: 110 2574 187 2869 Fragments arranged by size: 2869 2574 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5740 BssHII G'CGCG_C Cuts at: 1925 2030 1925 Size: 105 5635 BssSI C'ACGA_G Cuts at: 3800 4812 3800 Size: 1012 4728 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5740 BstBI TT'CG_AA Cuts at: 4544 4544 Size: 5740 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5740 BstXI CCAn_nnnn'nTGG Cuts at: 2308 2308 Size: 5740 BstYI r'GATC_y Cuts at: 1972 2157 2643 3070 3437 3822 3839 5291 5377 Size: 185 486 427 367 385 17 1452 86 Cuts at: 5377 5389 5735 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 486 427 385 367 346 185 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4249 Fragments arranged by size: 4249 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5001 Fragments arranged by size: 5001 683 56 BtsI GCAGTG_nn' Cuts at: 2264 2854 3256 4050 4070 2264 Size: 590 402 794 20 3934 Fragments arranged by size: 3934 794 590 402 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5740 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5705 DraI TTT'AAA Cuts at: 3044 3044 Size: 5740 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4747 809 Size: 507 3431 1802 Fragments arranged by size: 3431 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2012 2247 2360 Size: 54 1425 50 275 18 182 235 113 Cuts at: 2360 2384 3187 4076 5705 8 Size: 24 803 889 1629 43 Fragments arranged by size: 1629 1425 889 803 275 235 182 113 54 50 43 24 18 EagI C'GGCC_G Cuts at: 1537 1812 2012 3187 1537 Size: 275 200 1175 4090 Fragments arranged by size: 4090 1175 275 200 EarI CTCTTCn'nnn_ Cuts at: 1353 2054 3670 1353 Size: 701 1616 3423 Fragments arranged by size: 3423 1616 701 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2408 4327 4701 4847 387 Size: 1064 138 819 1919 374 146 1280 Fragments arranged by size: 1919 1280 1064 819 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2284 2579 3016 3803 4400 5187 5455 Size: 393 652 295 437 787 597 787 268 Cuts at: 5455 1239 Size: 1524 Fragments arranged by size: 1524 787 787 652 597 437 393 295 268 EcoO109I rG'GnC_Cy Cuts at: 2242 2256 2554 2795 2810 2903 2904 2917 2242 Size: 14 298 241 15 93 1 13 5065 Fragments arranged by size: 5065 298 241 93 15 14 13 1 EcoRI G'AATT_C Cuts at: 3049 3427 3049 Size: 378 5362 FspI TGC'GCA Cuts at: 2378 4246 2378 Size: 1868 3872 HaeII r_GCGC'y Cuts at: 1678 4887 1678 Size: 3209 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2782 2814 4381 4413 4455 Size: 32 135 32 1210 32 1567 32 42 Cuts at: 4455 4487 1373 Size: 32 2626 Fragments arranged by size: 2626 1567 1210 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3084 3330 669 Size: 988 281 1146 246 3079 Fragments arranged by size: 3079 1146 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5740 HpaI GTT'AAC Cuts at: 3330 3330 Size: 5740 KpnI G_GTAC'C Cuts at: 2949 3065 2949 Size: 116 5624 MfeI C'AATT_G Cuts at: 3339 3339 Size: 5740 MluI A'CGCG_T Cuts at: 5616 5616 Size: 5740 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2107 2346 4854 5038 1305 Size: 802 239 2508 184 2007 Fragments arranged by size: 2508 2007 802 239 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2249 10 Size: 54 1425 343 417 3501 Fragments arranged by size: 3501 1425 417 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2441 2487 2534 3698 4057 4216 518 Size: 648 1275 46 47 1164 359 159 2042 Fragments arranged by size: 2042 1275 1164 648 359 159 47 46 MspA1I CmG'CkG Cuts at: 1606 2253 2304 2335 3156 3833 4981 5226 1606 Size: 647 51 31 821 677 1148 245 2120 Fragments arranged by size: 2120 1148 821 677 647 245 51 31 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4249 Fragments arranged by size: 4249 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5740 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5740 NotI GC'GGCC_GC Cuts at: 3187 3187 Size: 5740 NspI r_CATG'y Cuts at: 1132 1229 3098 4643 1132 Size: 97 1869 1545 2229 Fragments arranged by size: 2229 1869 1545 97 PasI CC'CwG_GG Cuts at: 1348 2506 2551 1348 Size: 1158 45 4537 Fragments arranged by size: 4537 1158 45 PciI A'CATG_T Cuts at: 4639 4639 Size: 5740 PflMI CCAn_nnn'nTGG Cuts at: 1108 2557 1108 Size: 1449 4291 PfoI T'CCnGG_A Cuts at: 2121 2569 2121 Size: 448 5292 PmeI GTTT'AAAC Cuts at: 3044 3044 Size: 5740 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3809 3841 5322 5354 3809 Size: 32 1481 32 4195 Fragments arranged by size: 4195 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2795 2917 2795 Size: 122 5618 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5740 PsiI TTA'TAA Cuts at: 3310 3310 Size: 5740 PspOMI G'GGCC_C Cuts at: 2241 2255 2903 2241 Size: 14 648 5078 Fragments arranged by size: 5078 648 14 PspXI vC'TCGA_Gb Cuts at: 2648 2648 Size: 5740 PstI C_TGCA'G Cuts at: 830 2334 2809 3092 830 Size: 1504 475 283 3478 Fragments arranged by size: 3478 1504 475 283 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5740 SalI G'TCGA_C Cuts at: 1936 3082 1936 Size: 1146 4594 SapI GCTCTTCn'nnn_ Cuts at: 2054 2054 Size: 5740 SbfI CC_TGCA'GG Cuts at: 3092 3092 Size: 5740 ScaI AGT'ACT Cuts at: 3988 5700 3988 Size: 1712 4028 SexAI A'CCwGG_T Cuts at: 2853 2853 Size: 5740 SfcI C'TryA_G Cuts at: 826 1045 2330 2805 3088 4223 4904 5095 826 Size: 219 1285 475 283 1135 681 191 1471 Fragments arranged by size: 1471 1285 1135 681 475 283 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5740 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5740 SmaI CCC'GGG Cuts at: 1872 2163 3067 1872 Size: 291 904 4545 Fragments arranged by size: 4545 904 291 SmlI C'TyrA_G Cuts at: 820 1017 1943 2648 3844 4745 5007 820 Size: 197 926 705 1196 901 262 1553 Fragments arranged by size: 1553 1196 926 901 705 262 197 SnaBI TAC'GTA Cuts at: 493 3531 493 Size: 3038 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5740 SphI G_CATG'C Cuts at: 1132 3098 1132 Size: 1966 3774 SspI AAT'ATT Cuts at: 5 52 3664 5 Size: 47 3612 2081 Fragments arranged by size: 3612 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5740 StyI C'CwwG_G Cuts at: 513 1065 2004 2682 3179 513 Size: 552 939 678 497 3074 Fragments arranged by size: 3074 939 678 552 497 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3767 3784 3937 4122 4567 325 Size: 1255 2187 17 153 185 445 1498 Fragments arranged by size: 2187 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2615 3986 5698 Size: 274 80 33 51 1443 638 1371 1712 Cuts at: 5698 96 Size: 138 Fragments arranged by size: 1712 1443 1371 638 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5700 5732 5700 Size: 32 5708 Tth111I GACn'n_nGTC Cuts at: 1316 2855 1316 Size: 1539 4201 XbaI T'CTAG_A Cuts at: 3076 3076 Size: 5740 XhoI C'TCGA_G Cuts at: 1943 2648 1943 Size: 705 5035 XmaI C'CCGG_G Cuts at: 1870 2161 3065 1870 Size: 291 904 4545 Fragments arranged by size: 4545 904 291 XmnI GAAnn'nnTTC Cuts at: 3869 3869 Size: 5740 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3548 276 Size: 53 83 186 936 2014 2468 Fragments arranged by size: 2468 2014 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PsrI PvuII RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI XcmI