################################## # NO CUT ENZYMES ( 84 enzymes) # ################################## Acc65I AccI AccIII AclI AcsI AflII AgeI Alw44I Aor51HI ApaLI ApoI AscI AseI AsnI Asp718I AvrII BamHI BciVI BclI BfrI BglII BlnI BsaBI BseAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI Bst1107I BstBI BstZ17I ClaI DraI Eco47III EcoRI EcoT22I FbaI FseI HincII HindII HindIII HpaI KpnI MamI MfeI MluI MroI MseI MunI NcoI NdeI NheI NotI NruI NsiI NspV PacI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI SalI SapI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI SspBI SspI SwaI Tru9I Tsp509I ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AatII 1573 GACGTC GACGT^C AflIII 71 ACGTGT A^C[GA][CT]GT AhdI 1915 GACGTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 1471 CCTGAGG CC^T[ACGT]AGG Asp700I 2376 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 1915 GACGTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 143 TGCGCA TGC^GCA BbeI 1347 GGCGCC GGCGC^C BfaI 1144 CTAG C^TAG BglI 1838 GCCCCGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsiEI 2055 CGGCCG CG[GA][CT]^CG BsmBI 1669 GAGACG CGTCTC(1/5) BsrBI 2065 GAGCGG CCGCTC(-3/-3) BsrFI 2057 GCCGGC [GA]^CCGG[CT] BssHII 1194 GCGCGC G^CGCGC BssSI 790 CACGAG CACGAG(-5/-1) BstXI 140 CCATGCGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 1471 CCTGAGG CC^T[ACGT]AGG Cfr10I 2057 GCCGGC [GA]^CCGG[CT] CpoI 864 CGGACCG CG^G[AT]CCG DraIII 2659 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1870 GACCAGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 2055 CGGCCG C^GGCCG Eam1105I 1915 GACGTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 2055 CGGCCG C^GGCCG Eco52I 2055 CGGCCG C^GGCCG Eco81I 1471 CCTGAGG CC^T[ACGT]AGG EcoNI 1017 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 2613 GATATC GAT^ATC FspI 143 TGCGCA TGC^GCA KasI 1347 GGCGCC G^GCGCC MaeI 1144 CTAG C^TAG NaeI 2057 GCCGGC GCC^GGC NarI 1347 GGCGCC GG^CGCC NgoMIV 2057 GCCGGC G^CCGGC NspI 1217 ACATGC [GA]CATG^[CT] PaeR7I 262 CTCGAG C^TCGAG PleI 2179 GAGTC GAGTC(4/5) RsrII 864 CGGACCG CG^G[AT]CCG SacI 2272 GAGCTC GAGCT^C SbfI 333 CCTGCAGG CCTGCA^GG SfoI 1347 GGCGCC GGC^GCC Sse8387I 333 CCTGCAGG CCTGCA^GG StuI 2246 AGGCCT AGG^CCT XbaI 1143 TCTAGA T^CTAGA XhoI 262 CTCGAG C^TCGAG XmnI 2376 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AspHI 2151 GAGCAC G[AT]GC[AT]^C 2272 GAGCTC BanI 1347 GGCGCC G^G[CT][GA]CC 2664 GGTGCC BbrPI 775 CACGTG CAC^GTG 2659 CACGTG BbsI 1441 GTCTTC GAAGAC(2/6) 1879 GTCTTC BbvCI 999 GCTGAGG CCTCAGC(-5/-2) 2362 GCTGAGG BcgI 2178 CGAGTCCCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1715 GCAAAGCCATCG BlpI 2269 GCTGAGC GC^T[ACGT]AGC 2350 GCTCAGC Bpu1102I 2269 GCTGAGC GC^T[ACGT]AGC 2350 GCTCAGC BpuAI 1441 GTCTTC GAAGAC(2/6) 1879 GTCTTC BsaI 1819 GAGACC GGTCTC(1/5) 1044 GGTCTC BsiHKAI 2151 GAGCAC G[AT]GC[AT]^C 2272 GAGCTC BstAPI 536 GCAAGGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 987 GCAGGGGCTGC BstEII 150 GGTGACC G^GT[ACGT]ACC 591 GGTCACC BstPI 150 GGTGACC G^GT[ACGT]ACC 591 GGTCACC BstYI 266 AGATCC [GA]^GATC[CT] 2075 AGATCC CelII 2269 GCTGAGC GC^T[ACGT]AGC 2350 GCTCAGC EarI 2521 CTCTTC CTCTTC(1/4) 10 GAAGAG EcoO65I 150 GGTGACC G^GT[ACGT]ACC 591 GGTCACC Ksp632I 2521 CTCTTC CTCTTC(1/4) 10 GAAGAG KspI 1077 CCGCGG CCGC^GG 2052 CCGCGG MflI 266 AGATCC [GA]^GATC[CT] 2075 AGATCC PmaCI 775 CACGTG CAC^GTG 2659 CACGTG PmlI 775 CACGTG CAC^GTG 2659 CACGTG SacII 1077 CCGCGG CCGC^GG 2052 CCGCGG TfiI 849 GATTC G^A[AT]TC 879 GAATC XcmI 815 CCATCACCACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1106 CCAAAGTGGAGGTGG XhoII 266 AGATCC [GA]^GATC[CT] 2075 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 100 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI ApaI AspI AvaI AvaII BalI BanII BbvI BcnI BmyI BpmI BsaAI BsaHI BsaJI BsaWI BseRI BsgI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PflFI PflMI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StyI TaqI TseI Tsp45I TspRI Tth111I TthHB8I Van91I XmaCI XmaI