(Circular) (Six-base) MAPSORT of: pFN21AE4587.seq Check: 3675 from: 1 to: 7384 pFN21AE4587 7384 bp With 182 enzymes: SgfI * March 28, 2012 14:18 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2332 3400 2332 Size: 1068 6316 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3582 5194 278 Size: 53 83 186 936 2046 1612 2468 Fragments arranged by size: 2468 2046 1612 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4727 1937 Size: 2790 4594 Acc65I G'GTAC_C Cuts at: 4705 4705 Size: 7384 AclI AA'CG_TT Cuts at: 1563 5511 5884 1563 Size: 3948 373 3063 Fragments arranged by size: 3948 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2034 2701 5447 6831 7099 1632 Size: 402 667 2746 1384 268 1917 Fragments arranged by size: 2746 1917 1384 667 402 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7187 AflIII A'CryG_T Cuts at: 1203 2076 6283 7260 1203 Size: 873 4207 977 1327 Fragments arranged by size: 4207 1327 977 873 AgeI A'CCGG_T Cuts at: 6215 6215 Size: 7384 AhdI GACnn_n'nnGTC Cuts at: 3925 6113 3925 Size: 2188 5196 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7384 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3188 3239 3875 1184 Size: 2004 51 636 4693 Fragments arranged by size: 4693 2004 636 51 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2946 2978 2946 Size: 32 7352 AlwNI CAG_nnn'CTG Cuts at: 2524 2998 3022 4042 4363 4477 4576 5127 6699 Size: 474 24 1020 321 114 99 551 1572 Cuts at: 6699 2524 Size: 3209 Fragments arranged by size: 3209 1572 1020 551 474 321 114 99 24 ApaI G_GGCC'C Cuts at: 2539 2837 3013 3845 4330 4563 4602 2539 Size: 298 176 832 485 233 39 5321 Fragments arranged by size: 5321 832 485 298 233 176 39 ApaLI G'TGCA_C Cuts at: 5441 6597 5441 Size: 1156 6228 ApoI r'AATT_y Cuts at: 1493 4693 4919 5071 7180 1493 Size: 3200 226 152 2109 1697 Fragments arranged by size: 3200 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5938 160 Size: 872 4906 1606 Fragments arranged by size: 4906 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7384 AvaI C'yCGr_G Cuts at: 1870 1943 2202 2267 3266 3735 3870 3954 4323 Size: 73 259 65 999 469 135 84 369 Cuts at: 4323 4562 4709 1870 Size: 239 147 4545 Fragments arranged by size: 4545 999 469 369 259 239 147 135 84 73 65 BamHI G'GATC_C Cuts at: 4714 5081 4714 Size: 367 7017 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3352 4669 4705 6160 618 Size: 325 217 627 1565 1317 36 1455 1842 Fragments arranged by size: 1842 1565 1455 1317 627 325 217 36 BanII G_rGCy'C Cuts at: 1391 2539 2698 2837 3013 3439 3845 4281 4330 Size: 1148 159 139 176 426 406 436 49 Cuts at: 4330 4563 4602 4703 1391 Size: 233 39 101 4072 Fragments arranged by size: 4072 1148 436 426 406 233 176 159 139 101 49 39 BbeI G_GCGC'C Cuts at: 3356 3356 Size: 7384 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3439 3877 928 Size: 971 1540 438 4435 Fragments arranged by size: 4435 1540 971 438 BbvCI CC'TCA_GC Cuts at: 3005 4368 3005 Size: 1363 6021 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3709 3743 4172 4206 5575 5609 1903 Size: 34 1772 34 429 34 1369 34 3678 Fragments arranged by size: 3678 1772 1369 429 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5276 6486 5276 Size: 1210 6174 BclI T'GATC_A Cuts at: 1548 1548 Size: 7384 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3849 5995 136 Size: 107 122 71 631 605 2177 2146 1525 Fragments arranged by size: 2177 2146 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7379 7379 Size: 7384 BlpI GC'TnA_GC Cuts at: 4275 4356 4801 4275 Size: 81 445 6858 Fragments arranged by size: 6858 445 81 Bme1580I G_kGCm'C Cuts at: 1790 2539 2820 2837 3013 3393 3845 4330 4563 Size: 749 281 17 176 380 452 485 233 Cuts at: 4563 4602 5445 6601 1790 Size: 39 843 1156 2573 Fragments arranged by size: 2573 1156 843 749 485 452 380 281 233 176 39 17 BmgBI CAC'GTC Cuts at: 1318 2171 3922 1318 Size: 853 1751 4780 Fragments arranged by size: 4780 1751 853 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2748 4621 5115 Size: 416 53 83 399 24 1331 1873 494 Cuts at: 5115 6073 442 Size: 958 1753 Fragments arranged by size: 1873 1753 1331 958 494 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7384 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3067 4371 3067 Size: 1304 6080 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2918 3044 3113 3326 4064 4151 4265 4355 Size: 1679 126 69 213 738 87 114 90 Cuts at: 4355 4361 4454 4674 6044 1239 Size: 6 93 220 1370 2579 Fragments arranged by size: 2579 1679 1370 738 220 213 126 114 93 90 87 69 6 Bpu10I CC'TnA_GC Cuts at: 2531 3005 3456 4368 4635 2531 Size: 474 451 912 267 5280 Fragments arranged by size: 5280 912 474 451 267 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4088 4629 5509 6374 6672 4088 Size: 541 880 865 298 4800 Fragments arranged by size: 4800 880 865 541 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3055 3818 6047 882 Size: 617 1556 763 2229 2219 Fragments arranged by size: 2229 2219 1556 763 617 BsaAI yAC'GTr Cuts at: 493 1204 2454 2782 4666 5175 493 Size: 711 1250 328 1884 509 2702 Fragments arranged by size: 2702 1884 1250 711 509 328 BsaBI GATnn'nnATC Cuts at: 7378 7378 Size: 7384 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2655 3353 3579 3960 Size: 53 83 186 936 1122 698 226 381 Cuts at: 3960 5191 5573 6208 275 Size: 1231 382 635 1451 Fragments arranged by size: 1451 1231 1122 936 698 635 382 381 226 186 83 53 BsaWI w'CCGG_w Cuts at: 2872 3188 3657 5817 6215 6489 6636 7047 2872 Size: 316 469 2160 398 274 147 411 3209 Fragments arranged by size: 3209 2160 469 411 398 316 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2397 2427 4169 4199 1178 Size: 30 165 30 994 30 1742 30 4363 Fragments arranged by size: 4363 1742 994 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2648 2759 2813 3041 3056 3143 3290 3494 Size: 1462 111 54 228 15 87 147 204 Cuts at: 3494 1186 Size: 5076 Fragments arranged by size: 5076 1462 228 204 147 111 87 54 15 BseYI C'CCAG_C Cuts at: 2550 2620 2806 2890 3062 3469 3509 3556 4329 Size: 70 186 84 172 407 40 47 773 Cuts at: 4329 4415 4442 4549 4601 6587 7083 2550 Size: 86 27 107 52 1986 496 2851 Fragments arranged by size: 2851 1986 773 496 407 186 172 107 86 84 70 52 47 40 27 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2164 2669 2783 3194 3428 3944 1163 Size: 573 428 505 114 411 234 516 4603 Fragments arranged by size: 4603 573 516 505 428 411 234 114 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4063 4834 5595 6623 1540 Size: 275 186 2062 771 761 1028 2301 Fragments arranged by size: 2301 2062 1028 771 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4160 4281 5445 5530 6601 4160 Size: 121 1164 85 1071 4943 Fragments arranged by size: 4943 1164 1071 121 85 BsmI GAATG_Cn' Cuts at: 4895 4988 4895 Size: 93 7291 BsmBI CGTCTCn'nnnn_ Cuts at: 3668 3668 Size: 7384 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2539 2698 2820 2837 3013 3393 3439 Size: 399 749 159 122 17 176 380 46 Cuts at: 3439 3845 4160 4281 4330 4563 4602 4703 5445 Size: 406 315 121 49 233 39 101 742 Cuts at: 5445 5530 6601 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 749 742 406 399 380 315 233 176 159 122 121 101 85 49 46 39 17 BspEI T'CCGG_A Cuts at: 7047 7047 Size: 7384 BspHI T'CATG_A Cuts at: 5273 7003 5273 Size: 1730 5654 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2332 3400 4447 4739 844 Size: 1488 1068 1047 292 3489 Fragments arranged by size: 3489 1488 1068 1047 292 BsrBI CCG'CTC Cuts at: 1394 1823 4072 5271 1394 Size: 429 2249 1199 3507 Fragments arranged by size: 3507 2249 1199 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5879 6053 65 Size: 1183 4631 174 1396 Fragments arranged by size: 4631 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4062 6028 6215 1700 Size: 110 2252 1966 187 2869 Fragments arranged by size: 2869 2252 1966 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7384 BssHII G'CGCG_C Cuts at: 1925 3199 1925 Size: 1274 6110 BssSI C'ACGA_G Cuts at: 2795 5444 6456 2795 Size: 2649 1012 3723 Fragments arranged by size: 3723 2649 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2547 2998 794 Size: 1753 451 5180 Fragments arranged by size: 5180 1753 451 BstBI TT'CG_AA Cuts at: 6188 6188 Size: 7384 BstEII G'GTnAC_C Cuts at: 1184 2155 2596 1184 Size: 971 441 5972 Fragments arranged by size: 5972 971 441 BstXI CCAn_nnnn'nTGG Cuts at: 2152 2152 Size: 7384 BstYI r'GATC_y Cuts at: 1972 2271 4080 4714 5081 5466 5483 6935 7021 Size: 299 1809 634 367 385 17 1452 86 Cuts at: 7021 7033 7379 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1809 1452 634 385 367 346 299 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3477 3477 Size: 7384 BtgI C'CryG_G Cuts at: 513 1065 2004 3082 3750 4057 4149 513 Size: 552 939 1078 668 307 92 3748 Fragments arranged by size: 3748 1078 939 668 552 307 92 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3712 487 Size: 683 56 2486 4159 Fragments arranged by size: 4159 2486 683 56 BtsI GCAGTG_nn' Cuts at: 2358 3804 3919 4900 5694 5714 2358 Size: 1446 115 981 794 20 4028 Fragments arranged by size: 4028 1446 981 794 115 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7384 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7349 DraI TTT'AAA Cuts at: 4688 4688 Size: 7384 DraIII CAC_nnn'GTG Cuts at: 4669 4669 Size: 7384 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3881 6391 809 Size: 507 2565 2510 1802 Fragments arranged by size: 2565 2510 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2187 3678 3984 Size: 54 1425 50 275 18 357 1491 306 Cuts at: 3984 4020 4060 4146 4257 4590 4831 5720 7349 Size: 36 40 86 111 333 241 889 1629 Cuts at: 7349 8 Size: 43 Fragments arranged by size: 1629 1491 1425 889 357 333 306 275 241 111 86 54 50 43 40 36 18 EagI C'GGCC_G Cuts at: 1537 1812 4060 4831 1537 Size: 275 2248 771 4090 Fragments arranged by size: 4090 2248 771 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2010 4532 5314 1353 Size: 657 2522 782 3423 Fragments arranged by size: 3423 2522 782 657 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2716 3614 4190 5971 6345 6491 Size: 1064 138 1127 898 576 1781 374 146 Cuts at: 6491 387 Size: 1280 Fragments arranged by size: 1781 1280 1127 1064 898 576 374 146 138 EcoICRI GAG'CTC Cuts at: 4279 4279 Size: 7384 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2034 2701 2918 3044 3113 3326 4064 Size: 393 402 667 217 126 69 213 738 Cuts at: 4064 4151 4265 4355 4361 4454 4674 5447 6044 Size: 87 114 90 6 93 220 773 597 Cuts at: 6044 6831 7099 1239 Size: 787 268 1524 Fragments arranged by size: 1524 787 773 738 667 597 402 393 268 220 217 213 126 114 93 90 87 69 6 EcoNI CCTnn'n_nnAGG Cuts at: 3026 3026 Size: 7384 EcoO109I rG'GnC_Cy Cuts at: 2287 2444 2535 2834 3009 3010 3298 3473 3560 Size: 157 91 299 175 1 288 175 87 Cuts at: 3560 3664 3841 3842 3867 3951 4412 4429 4559 Size: 104 177 1 25 84 461 17 130 Cuts at: 4559 4639 2287 Size: 80 5032 Fragments arranged by size: 5032 461 299 288 177 175 175 157 130 104 91 87 84 80 25 17 1 1 EcoRI G'AATT_C Cuts at: 4693 5071 4693 Size: 378 7006 EcoRV GAT'ATC Cuts at: 4620 4620 Size: 7384 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2267 2267 Size: 7384 FspI TGC'GCA Cuts at: 2150 5890 2150 Size: 3740 3644 FspAI rTGC'GCAy Cuts at: 2150 2150 Size: 7384 HaeII r_GCGC'y Cuts at: 1678 2381 3136 3356 6531 1678 Size: 703 755 220 3175 2531 Fragments arranged by size: 3175 2531 755 703 220 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3449 3481 3911 3943 4169 Size: 32 135 32 1877 32 430 32 226 Cuts at: 4169 4201 4604 4636 6025 6057 6099 6131 1373 Size: 32 403 32 1389 32 42 32 2626 Fragments arranged by size: 2626 1877 1389 430 403 226 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4728 4974 669 Size: 988 281 2790 246 3079 Fragments arranged by size: 3079 2790 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7384 HpaI GTT'AAC Cuts at: 4974 4974 Size: 7384 KasI G'GCGC_C Cuts at: 3352 3352 Size: 7384 KpnI G_GTAC'C Cuts at: 4709 4709 Size: 7384 MfeI C'AATT_G Cuts at: 4983 4983 Size: 7384 MluI A'CGCG_T Cuts at: 7260 7260 Size: 7384 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2848 2964 6498 6682 1305 Size: 1543 116 3534 184 2007 Fragments arranged by size: 3534 2007 1543 184 116 MscI TGG'CCA Cuts at: 10 64 1489 1832 2189 3986 4259 4592 10 Size: 54 1425 343 357 1797 273 333 2802 Fragments arranged by size: 2802 1797 1425 357 343 333 273 54 MslI CAynn'nnrTG Cuts at: 518 1166 2150 3170 3221 4148 5342 5701 5860 Size: 648 984 1020 51 927 1194 359 159 Cuts at: 5860 518 Size: 2042 Fragments arranged by size: 2042 1194 1020 984 927 648 359 159 51 MspA1I CmG'CkG Cuts at: 1606 2624 2890 2950 3084 3509 3625 3982 4059 Size: 1018 266 60 134 425 116 357 77 Cuts at: 4059 4333 4363 4800 5477 6625 6870 1606 Size: 274 30 437 677 1148 245 2120 Fragments arranged by size: 2120 1148 1018 677 437 425 357 274 266 245 134 116 77 60 30 NaeI GCC'GGC Cuts at: 4064 4064 Size: 7384 NarI GG'CG_CC Cuts at: 3353 3353 Size: 7384 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5893 Fragments arranged by size: 5893 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7384 NgoMIV G'CCGG_C Cuts at: 4062 4062 Size: 7384 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7384 NotI GC'GGCC_GC Cuts at: 4831 4831 Size: 7384 NspI r_CATG'y Cuts at: 1132 1229 3226 4742 6287 1132 Size: 97 1997 1516 1545 2229 Fragments arranged by size: 2229 1997 1545 1516 97 PasI CC'CwG_GG Cuts at: 1348 2212 2306 2830 4477 4576 4607 1348 Size: 864 94 524 1647 99 31 4125 Fragments arranged by size: 4125 1647 864 524 99 94 31 PciI A'CATG_T Cuts at: 6283 6283 Size: 7384 PflMI CCAn_nnn'nTGG Cuts at: 1108 2059 2218 3349 4477 1108 Size: 951 159 1131 1128 4015 Fragments arranged by size: 4015 1131 1128 951 159 PfoI T'CCnGG_A Cuts at: 2709 2964 4083 4651 2709 Size: 255 1119 568 5442 Fragments arranged by size: 5442 1119 568 255 PmeI GTTT'AAAC Cuts at: 4688 4688 Size: 7384 PmlI CAC'GTG Cuts at: 2782 4666 2782 Size: 1884 5500 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3470 3502 5453 5485 6966 6998 3470 Size: 32 1951 32 1481 32 3856 Fragments arranged by size: 3856 1951 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2287 2444 3298 3473 3664 3951 4429 2287 Size: 157 854 175 191 287 478 5242 Fragments arranged by size: 5242 854 478 287 191 175 157 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7384 PsiI TTA'TAA Cuts at: 4954 4954 Size: 7384 PspOMI G'GGCC_C Cuts at: 2535 2833 3009 3841 4326 4559 4598 2535 Size: 298 176 832 485 233 39 5321 Fragments arranged by size: 5321 832 485 298 233 176 39 PstI C_TGCA'G Cuts at: 830 2343 3018 3624 4233 4736 830 Size: 1513 675 606 609 503 3478 Fragments arranged by size: 3478 1513 675 609 606 503 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7384 PvuII CAG'CTG Cuts at: 2890 2950 3509 3625 3982 4333 4363 2890 Size: 60 559 116 357 351 30 5911 Fragments arranged by size: 5911 559 357 351 116 60 30 RsrII CG'GwC_CG Cuts at: 2870 2870 Size: 7384 SacI G_AGCT'C Cuts at: 4281 4281 Size: 7384 SacII CC_GC'GG Cuts at: 3085 4060 3085 Size: 975 6409 SalI G'TCGA_C Cuts at: 1936 4726 1936 Size: 2790 4594 SbfI CC_TGCA'GG Cuts at: 2343 4736 2343 Size: 2393 4991 ScaI AGT'ACT Cuts at: 5632 7344 5632 Size: 1712 5672 SfcI C'TryA_G Cuts at: 826 1045 2339 3014 3620 4229 4732 5867 6548 Size: 219 1294 675 606 609 503 1135 681 Cuts at: 6548 6739 826 Size: 191 1471 Fragments arranged by size: 1471 1294 1135 681 675 609 606 503 219 191 SfoI GGC'GCC Cuts at: 3354 3354 Size: 7384 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7384 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7384 SmaI CCC'GGG Cuts at: 1872 3268 3737 3872 3956 4325 4711 1872 Size: 1396 469 135 84 369 386 4545 Fragments arranged by size: 4545 1396 469 386 369 135 84 SmlI C'TyrA_G Cuts at: 820 1017 1943 2267 4067 4644 5488 6389 6651 Size: 197 926 324 1800 577 844 901 262 Cuts at: 6651 820 Size: 1553 Fragments arranged by size: 1800 1553 926 901 844 577 324 262 197 SnaBI TAC'GTA Cuts at: 493 5175 493 Size: 4682 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7384 SphI G_CATG'C Cuts at: 1132 4742 1132 Size: 3610 3774 SrfI GCCC'GGGC Cuts at: 4325 4325 Size: 7384 SspI AAT'ATT Cuts at: 5 52 5308 5 Size: 47 5256 2081 Fragments arranged by size: 5256 2081 47 StuI AGG'CCT Cuts at: 1517 4253 1517 Size: 2736 4648 StyI C'CwwG_G Cuts at: 513 1065 2004 3348 4170 4254 4823 513 Size: 552 939 1344 822 84 569 3074 Fragments arranged by size: 3074 1344 939 822 569 552 84 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5411 5428 5581 5766 6211 325 Size: 1255 3831 17 153 185 445 1498 Fragments arranged by size: 3831 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4675 5630 7342 Size: 274 80 33 51 1443 2698 955 1712 Cuts at: 7342 96 Size: 138 Fragments arranged by size: 2698 1712 1443 955 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2397 2429 7344 7376 2397 Size: 32 4915 32 2405 Fragments arranged by size: 4915 2405 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2657 2864 3239 1316 Size: 1341 207 375 5461 Fragments arranged by size: 5461 1341 375 207 XbaI T'CTAG_A Cuts at: 3148 4720 3148 Size: 1572 5812 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2827 3118 2827 Size: 291 7093 XhoI C'TCGA_G Cuts at: 1943 2267 1943 Size: 324 7060 XmaI C'CCGG_G Cuts at: 1870 3266 3735 3870 3954 4323 4709 1870 Size: 1396 469 135 84 369 386 4545 Fragments arranged by size: 4545 1396 469 386 369 135 84 XmnI GAAnn'nnTTC Cuts at: 4385 5513 4385 Size: 1128 6256 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3580 5192 276 Size: 53 83 186 936 2046 1612 2468 Fragments arranged by size: 2468 2046 1612 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BfrBI BsiWI BstZ17I FseI NruI NsiI PacI PspXI PsrI SanDI SapI SexAI SfiI SwaI