Restriction map for
pFN21AE1596
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# NO CUT ENZYMES ( 140 enzymes) #
##################################
AatII Acc65I AccII AclI AcsI
AflII AhdI Alw44I AlwNI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BalI BamHI BbeI BbsI
BcgI BciVI BclI BcnI BfrI
BlnI BpuAI BsaBI BsgI BsiEI
BsiWI BspDI BspHI BspLU11I BsrBI
BsrDI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I CfoI ClaI CpoI DraI
DraIII DrdI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRI EcoRV FauI FbaI
FseI FspI HaeII HhaI HinP1I
HincII HindII HindIII HpaI KasI
KpnI Ksp632I KspI MamI MboII
MfeI MluI MluNI MscI MseI
MunI MvnI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NspI NspV PacI PflFI PflMI
PmeI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SnaBI SphI Sse8387I SspI
SwaI Tru9I Tsp509I Tth111I Van91I
XbaI XcmI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccI 706 GTCTAC GT^[AC][GT]AC
AccIII 1031 TCCGGA T^CCGGA
AcyI 1037 GACGCC G[GA]^CG[CT]C
AflIII 479 ACACGT A^C[GA][CT]GT
AgeI 950 ACCGGT A^CCGGT
AlwI 984 GATCC GGATC(4/5)
AocI 882 CCTGAGG CC^T[ACGT]AGG
ApaI 26 GGGCCC GGGCC^C
AspHI 132 GAGCAC G[AT]GC[AT]^C
BanII 26 GGGCCC G[GA]GC[CT]^C
BbrPI 480 CACGTG CAC^GTG
BbvCI 666 CCTCAGC CCTCAGC(-5/-2)
BglI 1048 GCCTACCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 795 AGATCT A^GATCT
BlpI 898 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 898 GCTCAGC GC^T[ACGT]AGC
BsaAI 480 CACGTG [CT]AC^GT[GA]
BsaHI 1037 GACGCC G[GA]^CG[CT]C
BsaI 463 GAGACC GGTCTC(1/5)
BseAI 1031 TCCGGA T^CCGGA
BsiHKAI 132 GAGCAC G[AT]GC[AT]^C
BsmBI 1035 GAGACG CGTCTC(1/5)
BsmI 780 GAATGC GAATGC(1/-1)
Bsp1407I 542 TGTACA T^GTACA
BspEI 1031 TCCGGA T^CCGGA
BsrFI 950 ACCGGT [GA]^CCGG[CT]
BsrGI 542 TGTACA T^GTACA
BstYI 795 AGATCT [GA]^GATC[CT]
Bsu36I 882 CCTGAGG CC^T[ACGT]AGG
Cac8I 6 GCTGGC GC[ACGT]^[ACGT]GC
CelII 898 GCTCAGC GC^T[ACGT]AGC
Cfr10I 950 ACCGGT [GA]^CCGG[CT]
Eco81I 882 CCTGAGG CC^T[ACGT]AGG
EcoT22I 782 ATGCAT ATGCA^T
HgaI 1037 GACGC GACGC(5/10)
Hin1I 1037 GACGCC G[GA]^CG[CT]C
MflI 795 AGATCT [GA]^GATC[CT]
MroI 1031 TCCGGA T^CCGGA
NcoI 255 CCATGG C^CATGG
NsiI 782 ATGCAT ATGCA^T
PaeR7I 837 CTCGAG C^TCGAG
PinAI 950 ACCGGT A^CCGGT
PmaCI 480 CACGTG CAC^GTG
PmlI 480 CACGTG CAC^GTG
SmlI 837 CTCGAG C^T[CT][GA]AG
SpeI 340 ACTAGT A^CTAGT
SspBI 542 TGTACA T^GTACA
StuI 566 AGGCCT AGG^CCT
XhoI 837 CTCGAG C^TCGAG
XhoII 795 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AciI 1046 CCGC CCGC(-3/-1)
724 GCGG
AvaI 21 CTCGGG C^[CT]CG[GA]G
837 CTCGAG
BanI 202 GGCACC G^G[CT][GA]CC
625 GGTGCC
BfaI 341 CTAG C^TAG
446 CTAG
BpmI 691 CTCCAG CTGGAG(16/14)
889 CTCCAG
BsaWI 950 ACCGGT [AT]^CCGG[AT]
1031 TCCGGA
BseRI 997 CTCCTC GAGGAG(10/8)
560 GAGGAG
BsmFI 55 GGGAC GGGAC(10/14)
306 GGGAC
BsoBI 21 CTCGGG C^[CT]CG[GA]G
837 CTCGAG
BspMI 638 ACCTGC ACCTGC(4/8)
872 ACCTGC
DpnII 796 GATC ^GATC
984 GATC
HapII 951 CCGG C^CGG
1032 CCGG
HpaII 951 CCGG C^CGG
1032 CCGG
MaeI 341 CTAG C^TAG
446 CTAG
MaeII 263 ACGT A^CGT
481 ACGT
MboI 796 GATC ^GATC
984 GATC
MslI 346 CATGGAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
720 CATGGCGGTG
MspA1I 857 CAGCTG C[AC]G^C[GT]G
901 CAGCTG
MspI 951 CCGG C^CGG
1032 CCGG
NdeII 796 GATC ^GATC
984 GATC
PleI 441 GAGTC GAGTC(4/5)
536 GAGTC
PpuMI 306 GGGACCC [GA]G^G[AT]CC[CT]
686 AGGACCT
PvuII 857 CAGCTG CAG^CTG
901 CAGCTG
Sau3AI 796 GATC ^GATC
984 GATC
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AfaI AluI AvaII BbvI BmyI
BsaJI BsiYI BslI BsmAI Bsp1286I
BsrI BssKI BstF5I BstNI Cfr13I
DdeI DraII DsaI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HinfI
HphI ItaI MaeIII MnlI MvaI
MwoI NlaIII NlaIV RsaI Sau96I
ScrFI SfaNI StyI TaqI TfiI
TseI Tsp45I TspRI TthHB8I