(Circular) (Six-base) MAPSORT of: pFN21AE1596.seq Check: 170 from: 1 to: 5767 pFN21AE1596 5767 bp With 182 enzymes: SgfI * January 5, 2010 15:27 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2652 2652 Size: 5767 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3577 278 Size: 53 83 186 936 2041 2468 Fragments arranged by size: 2468 2041 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2712 3110 1937 Size: 775 398 4594 Fragments arranged by size: 4594 775 398 Acc65I G'GTAC_C Cuts at: 3088 3088 Size: 5767 AclI AA'CG_TT Cuts at: 1563 3894 4267 1563 Size: 2331 373 3063 Fragments arranged by size: 3063 2331 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2812 3830 5214 5482 1632 Size: 1180 1018 1384 268 1917 Fragments arranged by size: 1917 1384 1180 1018 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5570 AflIII A'CryG_T Cuts at: 1203 2484 4666 5643 1203 Size: 1281 2182 977 1327 Fragments arranged by size: 2182 1327 1281 977 AgeI A'CCGG_T Cuts at: 2955 4598 2955 Size: 1643 4124 AhdI GACnn_n'nnGTC Cuts at: 4496 4496 Size: 5767 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5767 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5767 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2482 2514 2482 Size: 32 5735 AlwNI CAG_nnn'CTG Cuts at: 3510 5082 3510 Size: 1572 4195 ApaI G_GGCC'C Cuts at: 2035 2035 Size: 5767 ApaLI G'TGCA_C Cuts at: 3824 4980 3824 Size: 1156 4611 ApoI r'AATT_y Cuts at: 1493 3076 3302 3454 5563 1493 Size: 1583 226 152 2109 1697 Fragments arranged by size: 2109 1697 1583 226 152 AseI AT'TA_AT Cuts at: 160 1032 4321 160 Size: 872 3289 1606 Fragments arranged by size: 3289 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5767 AvaI C'yCGr_G Cuts at: 1870 1943 2026 2842 3092 1870 Size: 73 83 816 250 4545 Fragments arranged by size: 4545 816 250 83 73 BamHI G'GATC_C Cuts at: 3097 3464 3097 Size: 367 5400 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2207 2630 3088 4543 618 Size: 325 217 627 420 423 458 1455 1842 Fragments arranged by size: 1842 1455 627 458 423 420 325 217 BanII G_rGCy'C Cuts at: 1391 2035 3086 1391 Size: 644 1051 4072 Fragments arranged by size: 4072 1051 644 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4796 BbvCI CC'TCA_GC Cuts at: 2672 2672 Size: 5767 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3958 3992 1903 Size: 34 2021 34 3678 Fragments arranged by size: 3678 2021 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3659 4869 3659 Size: 1210 4557 BclI T'GATC_A Cuts at: 1548 1548 Size: 5767 BfrBI ATG'CAT Cuts at: 2789 2789 Size: 5767 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3059 4378 136 Size: 107 122 71 631 605 1387 1319 1525 Fragments arranged by size: 1525 1387 1319 631 605 122 107 71 BglII A'GATC_T Cuts at: 2800 5762 2800 Size: 2962 2805 BlpI GC'TnA_GC Cuts at: 2904 3184 2904 Size: 280 5487 Bme1580I G_kGCm'C Cuts at: 1790 2035 2635 3828 4984 1790 Size: 245 600 1193 1156 2573 Fragments arranged by size: 2573 1193 1156 600 245 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5767 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2943 3498 4456 Size: 416 53 83 399 24 1526 555 958 Cuts at: 4456 442 Size: 1753 Fragments arranged by size: 1753 1526 958 555 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5767 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2681 2879 4427 1239 Size: 1442 198 1548 2579 Fragments arranged by size: 2579 1548 1442 198 Bpu10I CC'TnA_GC Cuts at: 2672 2717 2672 Size: 45 5722 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3892 4757 5055 3892 Size: 865 298 4604 Fragments arranged by size: 4604 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2462 4430 882 Size: 617 963 1968 2219 Fragments arranged by size: 2219 1968 963 617 BsaAI yAC'GTr Cuts at: 493 1204 2487 3558 493 Size: 711 1283 1071 2702 Fragments arranged by size: 2702 1283 1071 711 BsaBI GATnn'nnATC Cuts at: 5761 5761 Size: 5767 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3043 3574 3956 4591 Size: 53 83 186 936 1510 531 382 635 Cuts at: 4591 275 Size: 1451 Fragments arranged by size: 1510 1451 936 635 531 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2955 3036 4200 4598 4872 5019 5430 2955 Size: 81 1164 398 274 147 411 3292 Fragments arranged by size: 3292 1164 411 398 274 147 81 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2867 2897 1178 Size: 30 165 30 1464 30 4048 Fragments arranged by size: 4048 1464 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2580 2993 1186 Size: 1394 413 3960 Fragments arranged by size: 3960 1394 413 BseYI C'CCAG_C Cuts at: 2038 2502 4970 5466 2038 Size: 464 2468 496 2339 Fragments arranged by size: 2468 2339 496 464 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5194 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3217 3978 5006 1540 Size: 275 186 1216 761 1028 2301 Fragments arranged by size: 2301 1216 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2141 3828 3913 4984 2141 Size: 1687 85 1071 2924 Fragments arranged by size: 2924 1687 1071 85 BsmI GAATG_Cn' Cuts at: 2791 3278 3371 2791 Size: 487 93 5187 Fragments arranged by size: 5187 487 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3034 3034 Size: 5767 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2035 2141 2635 3086 3828 3913 4984 Size: 399 245 106 494 451 742 85 1071 Cuts at: 4984 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 494 451 399 245 106 85 BspEI T'CCGG_A Cuts at: 3036 5430 3036 Size: 2394 3373 BspHI T'CATG_A Cuts at: 3656 5386 3656 Size: 1730 4037 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2652 2886 3122 844 Size: 1808 234 236 3489 Fragments arranged by size: 3489 1808 236 234 BsrBI CCG'CTC Cuts at: 1394 1823 3654 1394 Size: 429 1831 3507 Fragments arranged by size: 3507 1831 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4262 4436 65 Size: 1183 3014 174 1396 Fragments arranged by size: 3014 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2955 4411 4598 1700 Size: 110 1145 1456 187 2869 Fragments arranged by size: 2869 1456 1145 187 110 BsrGI T'GTAC_A Cuts at: 96 2547 96 Size: 2451 3316 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5767 BssSI C'ACGA_G Cuts at: 3827 4839 3827 Size: 1012 4755 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5767 BstBI TT'CG_AA Cuts at: 4571 4571 Size: 5767 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5767 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 5767 BstYI r'GATC_y Cuts at: 1972 2800 3097 3464 3849 3866 5318 5404 5416 Size: 828 297 367 385 17 1452 86 12 Cuts at: 5416 5762 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 828 385 367 346 297 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2888 2888 Size: 5767 BtgI C'CryG_G Cuts at: 513 1065 2124 2218 2260 2361 513 Size: 552 1059 94 42 101 3919 Fragments arranged by size: 3919 1059 552 101 94 42 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5028 Fragments arranged by size: 5028 683 56 BtsI GCAGTG_nn' Cuts at: 2295 2385 3283 4077 4097 2295 Size: 90 898 794 20 3965 Fragments arranged by size: 3965 898 794 90 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5767 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5732 DraI TTT'AAA Cuts at: 3071 3071 Size: 5767 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4774 809 Size: 507 3458 1802 Fragments arranged by size: 3458 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3214 4103 5732 Size: 54 1425 50 275 18 1384 889 1629 Cuts at: 5732 8 Size: 43 Fragments arranged by size: 1629 1425 1384 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3214 1537 Size: 275 1402 4090 Fragments arranged by size: 4090 1402 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3697 1353 Size: 2344 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3040 4354 4728 4874 387 Size: 1064 138 1451 1314 374 146 1280 Fragments arranged by size: 1451 1314 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2681 2812 2879 3830 4427 5214 5482 Size: 393 1049 131 67 951 597 787 268 Cuts at: 5482 1239 Size: 1524 Fragments arranged by size: 1524 1049 951 787 597 393 268 131 67 EcoO109I rG'GnC_Cy Cuts at: 2031 2032 2312 2692 3061 2031 Size: 1 280 380 369 4737 Fragments arranged by size: 4737 380 369 280 1 EcoRI G'AATT_C Cuts at: 3076 3454 3076 Size: 378 5389 FspI TGC'GCA Cuts at: 4273 4273 Size: 5767 HaeII r_GCGC'y Cuts at: 1678 4914 1678 Size: 3236 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2221 2253 2867 2899 4408 Size: 32 135 32 649 32 614 32 1509 Cuts at: 4408 4440 4482 4514 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1509 649 614 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3111 3357 669 Size: 988 281 1173 246 3079 Fragments arranged by size: 3079 1173 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5767 HpaI GTT'AAC Cuts at: 3357 3357 Size: 5767 KpnI G_GTAC'C Cuts at: 3092 3092 Size: 5767 MfeI C'AATT_G Cuts at: 3366 3366 Size: 5767 MluI A'CGCG_T Cuts at: 5643 5643 Size: 5767 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2306 2396 4881 5065 1305 Size: 1001 90 2485 184 2007 Fragments arranged by size: 2485 2007 1001 184 90 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3945 Fragments arranged by size: 3945 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2355 2729 3725 4084 4243 518 Size: 648 1189 374 996 359 159 2042 Fragments arranged by size: 2042 1189 996 648 374 359 159 MspA1I CmG'CkG Cuts at: 1606 2864 2908 3183 3860 5008 5253 1606 Size: 1258 44 275 677 1148 245 2120 Fragments arranged by size: 2120 1258 1148 677 275 245 44 NcoI C'CATG_G Cuts at: 513 1065 2260 513 Size: 552 1195 4020 Fragments arranged by size: 4020 1195 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5767 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5767 NotI GC'GGCC_GC Cuts at: 3214 3214 Size: 5767 NsiI A_TGCA'T Cuts at: 2791 2791 Size: 5767 NspI r_CATG'y Cuts at: 1132 1229 3125 4670 1132 Size: 97 1896 1545 2229 Fragments arranged by size: 2229 1896 1545 97 PasI CC'CwG_GG Cuts at: 1348 2056 2557 2815 1348 Size: 708 501 258 4300 Fragments arranged by size: 4300 708 501 258 PciI A'CATG_T Cuts at: 4666 4666 Size: 5767 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5767 PmeI GTTT'AAAC Cuts at: 3071 3071 Size: 5767 PmlI CAC'GTG Cuts at: 2487 2487 Size: 5767 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3836 3868 5349 5381 3836 Size: 32 1481 32 4222 Fragments arranged by size: 4222 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2312 2692 2312 Size: 380 5387 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5767 PsiI TTA'TAA Cuts at: 3337 3337 Size: 5767 PspOMI G'GGCC_C Cuts at: 2031 2031 Size: 5767 PspXI vC'TCGA_Gb Cuts at: 2842 2842 Size: 5767 PstI C_TGCA'G Cuts at: 830 3119 830 Size: 2289 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5767 PvuII CAG'CTG Cuts at: 2864 2908 2864 Size: 44 5723 SalI G'TCGA_C Cuts at: 1936 3109 1936 Size: 1173 4594 SanDI GG'GwC_CC Cuts at: 2312 2312 Size: 5767 SbfI CC_TGCA'GG Cuts at: 3119 3119 Size: 5767 ScaI AGT'ACT Cuts at: 4015 5727 4015 Size: 1712 4055 SfcI C'TryA_G Cuts at: 826 1045 3115 4250 4931 5122 826 Size: 219 2070 1135 681 191 1471 Fragments arranged by size: 2070 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5767 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5767 SmaI CCC'GGG Cuts at: 1872 3094 1872 Size: 1222 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2842 3871 4772 5034 820 Size: 197 926 899 1029 901 262 1553 Fragments arranged by size: 1553 1029 926 901 899 262 197 SnaBI TAC'GTA Cuts at: 493 3558 493 Size: 3065 2702 SpeI A'CTAG_T Cuts at: 152 2345 152 Size: 2193 3574 SphI G_CATG'C Cuts at: 1132 3125 1132 Size: 1993 3774 SspI AAT'ATT Cuts at: 5 52 3691 5 Size: 47 3639 2081 Fragments arranged by size: 3639 2081 47 StuI AGG'CCT Cuts at: 1517 2573 1517 Size: 1056 4711 StyI C'CwwG_G Cuts at: 513 1065 2260 2463 2607 3206 513 Size: 552 1195 203 144 599 3074 Fragments arranged by size: 3074 1195 599 552 203 144 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2137 2436 3794 3811 3964 4149 4594 Size: 1255 557 299 1358 17 153 185 445 Cuts at: 4594 325 Size: 1498 Fragments arranged by size: 1498 1358 1255 557 445 299 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2547 2587 2736 Size: 274 80 33 51 1443 570 40 149 Cuts at: 2736 4013 5725 96 Size: 1277 1712 138 Fragments arranged by size: 1712 1443 1277 570 274 149 138 80 51 40 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2263 2295 2317 2349 2353 2385 2850 2882 5727 Size: 32 22 32 4 32 465 32 2845 Cuts at: 5727 5759 2263 Size: 32 2271 Fragments arranged by size: 2845 2271 465 32 32 32 32 32 22 4 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5767 XbaI T'CTAG_A Cuts at: 3103 3103 Size: 5767 XhoI C'TCGA_G Cuts at: 1943 2842 1943 Size: 899 4868 XmaI C'CCGG_G Cuts at: 1870 3092 1870 Size: 1222 4545 XmnI GAAnn'nnTTC Cuts at: 3896 3896 Size: 5767 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3575 276 Size: 53 83 186 936 2041 2468 Fragments arranged by size: 2468 2041 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BplI BsiWI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI