Restriction map for
pFN21AE1596
(Linear) (Six-base) MAPSORT of: KIEE1596.seq Check: 3167 from: 1 to: 1062
KIEE1596.orf
With 182 enzymes: SgfI *
January 5, 2010 13:57 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 647 1062
Size: 647 415
AccI GT'mk_AC
Cuts at: 0 707 1062
Size: 707 355
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 807 1062
Size: 807 255
AflIII A'CryG_T
Cuts at: 0 479 1062
Size: 479 583
AgeI A'CCGG_T
Cuts at: 0 950 1062
Size: 950 112
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 477 509 1062
Size: 477 32 553
Fragments arranged by size:
553 477 32
ApaI G_GGCC'C
Cuts at: 0 30 1062
Size: 30 1032
AvaI C'yCGr_G
Cuts at: 0 21 837 1062
Size: 21 816 225
Fragments arranged by size:
816 225 21
BanI G'GyrC_C
Cuts at: 0 202 625 1062
Size: 202 423 437
Fragments arranged by size:
437 423 202
BanII G_rGCy'C
Cuts at: 0 30 1062
Size: 30 1032
BbvCI CC'TCA_GC
Cuts at: 0 667 1062
Size: 667 395
BfrBI ATG'CAT
Cuts at: 0 784 1062
Size: 784 278
BglI GCCn_nnn'nGGC
Cuts at: 0 1054 1062
Size: 1054 8
BglII A'GATC_T
Cuts at: 0 795 1062
Size: 795 267
BlpI GC'TnA_GC
Cuts at: 0 899 1062
Size: 899 163
Bme1580I G_kGCm'C
Cuts at: 0 30 630 1062
Size: 30 600 432
Fragments arranged by size:
600 432 30
BmrI ACTGGGnnnn_n'
Cuts at: 0 938 1062
Size: 938 124
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 676 874 1062
Size: 676 198 188
Fragments arranged by size:
676 198 188
Bpu10I CC'TnA_GC
Cuts at: 0 667 712 1062
Size: 667 45 350
Fragments arranged by size:
667 350 45
BsaI GGTCTCn'nnnn_
Cuts at: 0 457 1062
Size: 457 605
BsaAI yAC'GTr
Cuts at: 0 482 1062
Size: 482 580
BsaHI Gr'CG_yC
Cuts at: 0 1038 1062
Size: 1038 24
BsaWI w'CCGG_w
Cuts at: 0 950 1031 1062
Size: 950 81 31
Fragments arranged by size:
950 81 31
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 862 892 1062
Size: 862 30 170
Fragments arranged by size:
862 170 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 575 988 1062
Size: 575 413 74
Fragments arranged by size:
575 413 74
BseYI C'CCAG_C
Cuts at: 0 33 497 1062
Size: 33 464 565
Fragments arranged by size:
565 464 33
BsiHKAI G_wGCw'C
Cuts at: 0 136 1062
Size: 136 926
BsmI GAATG_Cn'
Cuts at: 0 786 1062
Size: 786 276
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1029 1062
Size: 1029 33
Bsp1286I G_dGCh'C
Cuts at: 0 30 136 630 1062
Size: 30 106 494 432
Fragments arranged by size:
494 432 106 30
BspEI T'CCGG_A
Cuts at: 0 1031 1062
Size: 1031 31
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 647 881 1062
Size: 647 234 181
Fragments arranged by size:
647 234 181
BsrFI r'CCGG_y
Cuts at: 0 950 1062
Size: 950 112
BsrGI T'GTAC_A
Cuts at: 0 542 1062
Size: 542 520
BstYI r'GATC_y
Cuts at: 0 795 1062
Size: 795 267
Bsu36I CC'TnA_GG
Cuts at: 0 883 1062
Size: 883 179
BtgI C'CryG_G
Cuts at: 0 119 213 255 356 1062
Size: 119 94 42 101 706
Fragments arranged by size:
706 119 101 94 42
BtsI GCAGTG_nn'
Cuts at: 0 290 380 1062
Size: 290 90 682
Fragments arranged by size:
682 290 90
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1035 1062
Size: 1035 27
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 676 807 874 1062
Size: 676 131 67 188
Fragments arranged by size:
676 188 131 67
EcoO109I rG'GnC_Cy
Cuts at: 0 26 27 307 687 1056 1062
Size: 26 1 280 380 369 6
Fragments arranged by size:
380 369 280 26 6 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 216 248 862 894 1062
Size: 216 32 614 32 168
Fragments arranged by size:
614 216 168 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 301 391 1062
Size: 301 90 671
Fragments arranged by size:
671 301 90
MslI CAynn'nnrTG
Cuts at: 0 350 724 1062
Size: 350 374 338
Fragments arranged by size:
374 350 338
MspA1I CmG'CkG
Cuts at: 0 859 903 1062
Size: 859 44 159
Fragments arranged by size:
859 159 44
NcoI C'CATG_G
Cuts at: 0 255 1062
Size: 255 807
NsiI A_TGCA'T
Cuts at: 0 786 1062
Size: 786 276
PasI CC'CwG_GG
Cuts at: 0 51 552 810 1062
Size: 51 501 258 252
Fragments arranged by size:
501 258 252 51
PmlI CAC'GTG
Cuts at: 0 482 1062
Size: 482 580
PpuMI rG'GwC_Cy
Cuts at: 0 307 687 1062
Size: 307 380 375
Fragments arranged by size:
380 375 307
PspOMI G'GGCC_C
Cuts at: 0 26 1062
Size: 26 1036
PspXI vC'TCGA_Gb
Cuts at: 0 837 1062
Size: 837 225
PvuII CAG'CTG
Cuts at: 0 859 903 1062
Size: 859 44 159
Fragments arranged by size:
859 159 44
SanDI GG'GwC_CC
Cuts at: 0 307 1062
Size: 307 755
SmlI C'TyrA_G
Cuts at: 0 837 1062
Size: 837 225
SpeI A'CTAG_T
Cuts at: 0 340 1062
Size: 340 722
StuI AGG'CCT
Cuts at: 0 568 1062
Size: 568 494
StyI C'CwwG_G
Cuts at: 0 255 458 602 1062
Size: 255 203 144 460
Fragments arranged by size:
460 255 203 144
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 132 431 1062
Size: 132 299 631
Fragments arranged by size:
631 299 132
TatI w'GTAC_w
Cuts at: 0 542 582 731 1062
Size: 542 40 149 331
Fragments arranged by size:
542 331 149 40
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 258 290 312 344 348 380 845 877
Size: 258 32 22 32 4 32 465 32
Cuts at: 877 1062
Size: 185
Fragments arranged by size:
465 258 185 32 32 32 32 22
4
XhoI C'TCGA_G
Cuts at: 0 837 1062
Size: 837 225
Enzymes that do cut:
AarI AccI AcuI AflIII AgeI AloI ApaI AvaI
BanI BanII BbvCI BfrBI BglI BglII BlpI Bme1580I
BmrI BpmI Bpu10I BsaI BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI
BsrFI BsrGI BstYI Bsu36I BtgI BtsI EciI Eco57MI
EcoO109I Hin4I MmeI MslI MspA1I NcoI NsiI PasI
PmlI PpuMI PspOMI PspXI PvuII SanDI SmlI SpeI
StuI StyI TaqII TatI TstI XhoI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AhdI AleI AlfI
AlwNI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbsI BcgI BciVI BclI BmgBI BmtI
BplI BpuEI BsaBI BsgI BsiEI BsiWI BspHI BsrBI
BsrDI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I
BtgZI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI
MluI MscI NaeI NarI NdeI NgoMIV NheI NotI
NruI NspI PacI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PsrI PstI PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SnaBI SphI SrfI SspI SwaI
Tth111I XbaI XcmI XmaI XmnI ZraI