################################## # NO CUT ENZYMES ( 188 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BanI BanII BbeI BbrPI BbsI BciVI BclI BcnI BfrI BglI BglII BlnI BmyI BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsiYI BslI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MslI MspA1I MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tsp509I Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AciI 251 CCGC CCGC(-3/-1) AfaI 435 GTAC GT^AC AlwNI 394 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG AvaII 113 GGACC G^G[AT]CC BamHI 297 GGATCC G^GATCC BbvCI 16 GCTGAGG CCTCAGC(-5/-2) BcgI 89 GCAGACTCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 38 CTAG C^TAG BlpI 259 GCTAAGC GC^T[ACGT]AGC BpmI 71 CTGGAG CTGGAG(16/14) Bpu1102I 259 GCTAAGC GC^T[ACGT]AGC BsmAI 150 GTCTC GTCTC(1/5) BsmBI 149 CGTCTC CGTCTC(1/5) BsrDI 417 CATTGC GCAATG(2/0) BstF5I 162 CATCC GGATG(2/0) BstXI 416 CCATTGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 297 GGATCC [GA]^GATC[CT] CelII 259 GCTAAGC GC^T[ACGT]AGC DpnII 298 GATC ^GATC DsaI 189 CCATGG C^C[GA][CT]GG EarI 64 GAAGAG CTCTTC(1/4) FauI 250 CCCGC CCCGC(4/6) FokI 162 CATCC GGATG(9/13) HinfI 92 GACTC G^A[ACGT]TC Ksp632I 64 GAAGAG CTCTTC(1/4) MaeI 38 CTAG C^TAG MaeII 326 ACGT A^CGT MboI 298 GATC ^GATC MboII 64 GAAGA GAAGA(8/7) MflI 297 GGATCC [GA]^GATC[CT] MseI 227 TTAA T^TAA NcoI 189 CCATGG C^CATGG NdeII 298 GATC ^GATC NspI 291 GCATGT [GA]CATG^[CT] PleI 92 GACTC GAGTC(4/5) RsaI 435 GTAC GT^AC Sau3AI 298 GATC ^GATC SfaNI 220 GATGC GCATC(5/9) SmlI 244 CTCAAG C^T[CT][GA]AG Tru9I 227 TTAA T^TAA TspRI 277 CACTG CA[GC]TG[ACGT][ACGT]^ XhoII 297 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 8 enzymes) # ################################### AlwI 298 GATCC GGATC(4/5) 297 GGATC BseRI 94 CTCCTC GAGGAG(10/8) 19 GAGGAG BsgI 274 CTGCAC GTGCAG(16/14) 379 GTGCAG Cac8I 249 GCCCGC GC[ACGT]^[ACGT]GC 381 GCAGGC HaeIII 186 GGCC GG^CC 426 GGCC MwoI 41 GCCTGTGGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 264 GCTGAGTGAGC NlaIV 113 GGACCC GG[ACGT]^[ACGT]CC 297 GGATCC Tsp45I 34 GTCAC ^GT[GC]AC 281 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 20 enzymes) # ######################################### AluI BbvI BsaJI BssKI BstNI Cfr13I DdeI EcoRII EcoT14I Fnu4HI HphI ItaI MaeIII MnlI MvaI NlaIII Sau96I ScrFI StyI TseI