Restriction map for
pFN21AE3590
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# NO CUT ENZYMES ( 143 enzymes) #
##################################
Acc65I AccIII AclI AcsI AflII
AgeI AhdI AocI ApaI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AvaII AvrII
BamHI BbeI BbrPI BbvCI BcgI
BciVI BclI BcnI BfrI BglII
BlnI BpmI BsaBI BseAI BsgI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrFI BsrGI BsrI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraI DraIII DrdI DsaI
EagI Eam1105I EclXI Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI HindIII HpaI KasI KpnI
KspI MamI MfeI MluI MroI
MseI MslI MunI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacII SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tru9I
Tsp509I Tth111I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 61 enzymes) #
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AccI 392 GTCGAC GT^[AC][GT]AC
AccII 13 CGCG CG^CG
AfaI 48 GTAC GT^AC
AflIII 396 ACGTGT A^C[GA][CT]GT
Alw44I 82 GTGCAC G^TGCAC
AlwI 561 GGATC GGATC(4/5)
AlwNI 194 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 82 GTGCAC G^TGCAC
AviII 317 TGCGCA TGC^GCA
BalI 137 TGGCCA TGG^CCA
BanII 487 GAGCTC G[GA]GC[CT]^C
BbsI 379 GAAGAC GAAGAC(2/6)
BglI 130 GCCTTCGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 442 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 442 GCTCAGC GC^T[ACGT]AGC
BpuAI 379 GAAGAC GAAGAC(2/6)
BsaI 158 GAGACC GGTCTC(1/5)
BseRI 328 GAGGAG GAGGAG(10/8)
BsmAI 158 GAGAC GTCTC(1/5)
BsmFI 185 GGGAC GGGAC(10/14)
BspMI 368 ACCTGC ACCTGC(4/8)
BsrBI 3 GAGCGG CCGCTC(-3/-3)
BstF5I 458 CATCC GGATG(2/0)
BstUI 13 CGCG CG^CG
BstYI 561 GGATCT [GA]^GATC[CT]
CelII 442 GCTCAGC GC^T[ACGT]AGC
DpnII 562 GATC ^GATC
DraII 102 GGGGCCT [GA]G^G[ACGT]CC[CT]
EaeI 137 TGGCCA [CT]^GGCC[GA]
EarI 490 CTCTTC CTCTTC(1/4)
EcoNI 324 CCTAGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 102 GGGGCCT [GA]G^G[ACGT]CC[CT]
FauI 5 GCGGG CCCGC(4/6)
FokI 458 CATCC GGATG(9/13)
FspI 317 TGCGCA TGC^GCA
HgaI 390 GCGTC GACGC(5/10)
HincII 392 GTCGAC GT[CT]^[GA]AC
HindII 392 GTCGAC GT[CT]^[GA]AC
HinfI 224 GAATC G^A[ACGT]TC
Ksp632I 490 CTCTTC CTCTTC(1/4)
MboI 562 GATC ^GATC
MflI 561 GGATCT [GA]^GATC[CT]
MluNI 137 TGGCCA TGG^CCA
MscI 137 TGGCCA TGG^CCA
MspA1I 594 CAGCTG C[AC]G^C[GT]G
MvnI 13 CGCG CG^CG
NdeII 562 GATC ^GATC
NlaIII 216 CATG CATG^
PvuII 594 CAGCTG CAG^CTG
RsaI 48 GTAC GT^AC
SacI 487 GAGCTC GAGCT^C
SalI 392 GTCGAC G^TCGAC
SapI 489 GCTCTTC GCTCTTC(1/4)
Sau3AI 562 GATC ^GATC
SfaNI 33 GATGC GCATC(5/9)
SfcI 591 CTACAG C^T[GA][CT]AG
TaqI 393 TCGA T^CGA
TfiI 224 GAATC G^A[AT]TC
TspRI 519 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 393 TCGA T^CGA
XhoII 561 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AatII 19 GACGTC GACGT^C
412 GACGTC
Aor51HI 74 AGCGCT AGC^GCT
203 AGCGCT
AspHI 82 GTGCAC G[AT]GC[AT]^C
487 GAGCTC
BanI 238 GGTGCC G^G[CT][GA]CC
355 GGCACC
BfaI 62 CTAG C^TAG
325 CTAG
BmyI 82 GTGCAC G[AGT]GC[ACT]^C
487 GAGCTC
BsaAI 49 TACGTG [CT]AC^GT[GA]
79 TACGTG
BsaJI 140 CCAAGG C^C[ACGT][ACGT]GG
242 CCAAGG
BsaWI 161 ACCGGA [AT]^CCGG[AT]
383 ACCGGA
BsiEI 87 CGACCG CG[GA][CT]^CG
152 CGGTCG
BsiHKAI 82 GTGCAC G[AT]GC[AT]^C
487 GAGCTC
Bsp1286I 82 GTGCAC G[AGT]GC[ACT]^C
487 GAGCTC
DdeI 200 CTGAG C^T[ACGT]AG
443 CTCAG
Eco47III 74 AGCGCT AGC^GCT
203 AGCGCT
EcoT14I 140 CCAAGG C^C[AT][AT]GG
242 CCAAGG
HaeII 74 AGCGCT [GA]GCGC^[CT]
203 AGCGCT
HapII 162 CCGG C^CGG
384 CCGG
HpaII 162 CCGG C^CGG
384 CCGG
MaeI 62 CTAG C^TAG
325 CTAG
MaeIII 169 GTGAC ^GT[ACGT]AC
365 GTGAC
MspI 162 CCGG C^CGG
384 CCGG
PflMI 474 CCAGAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
495 CCAGAAAGTGG
StyI 140 CCAAGG C^C[AT][AT]GG
242 CCAAGG
Tsp45I 169 GTGAC ^GT[GC]AC
365 GTGAC
Van91I 474 CCAGAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
495 CCAGAAAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AciI AcyI AluI BbvI BsaHI
BsiYI BslI BssKI BstNI Cac8I
CfoI Cfr13I EcoRII Fnu4HI HaeIII
HhaI Hin1I HinP1I HphI ItaI
MaeII MboII MnlI MvaI MwoI
NlaIV Sau96I ScrFI TseI