(Circular) (Six-base) MAPSORT of: pFN21AE1540.seq Check: 3760 from: 1 to: 5080 pFN21AE1540 5080 bp With 182 enzymes: SgfI * August 16, 2010 14:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2890 278 Size: 53 83 186 936 1354 2468 Fragments arranged by size: 2468 1354 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2423 1937 Size: 486 4594 Acc65I G'GTAC_C Cuts at: 2401 2401 Size: 5080 AclI AA'CG_TT Cuts at: 1563 3207 3580 1563 Size: 1644 373 3063 Fragments arranged by size: 3063 1644 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3143 4527 4795 1632 Size: 1511 1384 268 1917 Fragments arranged by size: 1917 1511 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4883 AflIII A'CryG_T Cuts at: 1203 3979 4956 1203 Size: 2776 977 1327 Fragments arranged by size: 2776 1327 977 AgeI A'CCGG_T Cuts at: 3911 3911 Size: 5080 AhdI GACnn_n'nnGTC Cuts at: 3809 3809 Size: 5080 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5080 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5080 AlwNI CAG_nnn'CTG Cuts at: 2044 2823 4395 2044 Size: 779 1572 2729 Fragments arranged by size: 2729 1572 779 ApaLI G'TGCA_C Cuts at: 3137 4293 3137 Size: 1156 3924 ApoI r'AATT_y Cuts at: 1493 2333 2389 2615 2767 4876 1493 Size: 840 56 226 152 2109 1697 Fragments arranged by size: 2109 1697 840 226 152 56 AseI AT'TA_AT Cuts at: 160 1032 3634 160 Size: 872 2602 1606 Fragments arranged by size: 2602 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5080 AvaI C'yCGr_G Cuts at: 1870 1943 2160 2405 1870 Size: 73 217 245 4545 Fragments arranged by size: 4545 245 217 73 BamHI G'GATC_C Cuts at: 2071 2410 2777 2071 Size: 339 367 4374 Fragments arranged by size: 4374 367 339 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2401 3856 618 Size: 325 217 627 614 1455 1842 Fragments arranged by size: 1842 1455 627 614 325 217 BanII G_rGCy'C Cuts at: 1391 2399 1391 Size: 1008 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4109 BbvCI CC'TCA_GC Cuts at: 2030 2030 Size: 5080 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3271 3305 1903 Size: 34 1334 34 3678 Fragments arranged by size: 3678 1334 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2972 4182 2972 Size: 1210 3870 BclI T'GATC_A Cuts at: 1548 1548 Size: 5080 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3691 136 Size: 107 122 71 631 605 2019 1525 Fragments arranged by size: 2019 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5075 5075 Size: 5080 BlpI GC'TnA_GC Cuts at: 2497 2497 Size: 5080 Bme1580I G_kGCm'C Cuts at: 1790 3141 4297 1790 Size: 1351 1156 2573 Fragments arranged by size: 2573 1351 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5080 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2811 3769 442 Size: 416 53 83 399 24 1394 958 1753 Fragments arranged by size: 1753 1394 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5080 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3740 1239 Size: 2501 2579 Bpu10I CC'TnA_GC Cuts at: 2030 2030 Size: 5080 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2042 3205 4070 4368 2042 Size: 1163 865 298 2754 Fragments arranged by size: 2754 1163 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3743 882 Size: 617 2244 2219 Fragments arranged by size: 2244 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2871 493 Size: 711 1667 2702 Fragments arranged by size: 2702 1667 711 BsaBI GATnn'nnATC Cuts at: 5074 5074 Size: 5080 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2887 3269 3904 275 Size: 53 83 186 936 1354 382 635 1451 Fragments arranged by size: 1451 1354 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3513 3911 4185 4332 4743 3513 Size: 398 274 147 411 3850 Fragments arranged by size: 3850 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4855 Fragments arranged by size: 4855 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2030 2047 1186 Size: 844 17 4219 Fragments arranged by size: 4219 844 17 BseYI C'CCAG_C Cuts at: 4283 4779 4283 Size: 496 4584 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4507 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2530 3291 4319 1540 Size: 275 186 529 761 1028 2301 Fragments arranged by size: 2301 1028 761 529 275 186 BsiHKAI G_wGCw'C Cuts at: 3141 3226 4297 3141 Size: 85 1071 3924 Fragments arranged by size: 3924 1071 85 BsmI GAATG_Cn' Cuts at: 2591 2684 2591 Size: 93 4987 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2399 3141 3226 4297 1391 Size: 399 609 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 609 399 85 BspEI T'CCGG_A Cuts at: 4743 4743 Size: 5080 BspHI T'CATG_A Cuts at: 2969 4699 2969 Size: 1730 3350 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2435 844 Size: 1591 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2967 1394 Size: 429 1144 3507 Fragments arranged by size: 3507 1144 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3575 3749 65 Size: 1183 2327 174 1396 Fragments arranged by size: 2327 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3724 3911 1700 Size: 110 1914 187 2869 Fragments arranged by size: 2869 1914 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5080 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5080 BssSI C'ACGA_G Cuts at: 3140 4152 3140 Size: 1012 4068 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5080 BstBI TT'CG_AA Cuts at: 3884 3884 Size: 5080 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5080 BstYI r'GATC_y Cuts at: 1972 2071 2410 2777 3162 3179 4631 4717 4729 Size: 99 339 367 385 17 1452 86 12 Cuts at: 4729 5075 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 385 367 346 339 99 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3589 Fragments arranged by size: 3589 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4341 Fragments arranged by size: 4341 683 56 BtsI GCAGTG_nn' Cuts at: 2596 3390 3410 2596 Size: 794 20 4266 Fragments arranged by size: 4266 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5080 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5045 DraI TTT'AAA Cuts at: 2384 2384 Size: 5080 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4087 809 Size: 507 2771 1802 Fragments arranged by size: 2771 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2046 2527 3416 Size: 54 1425 50 275 18 216 481 889 Cuts at: 3416 5045 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 481 275 216 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2527 1537 Size: 275 715 4090 Fragments arranged by size: 4090 715 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3010 1353 Size: 1657 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 3667 4041 4187 387 Size: 1064 138 435 1643 374 146 1280 Fragments arranged by size: 1643 1280 1064 435 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3143 3740 4527 4795 1239 Size: 393 1511 597 787 268 1524 Fragments arranged by size: 1524 1511 787 597 393 268 EcoRI G'AATT_C Cuts at: 2389 2767 2389 Size: 378 4702 FspI TGC'GCA Cuts at: 3586 3586 Size: 5080 HaeII r_GCGC'y Cuts at: 1678 2030 4227 1678 Size: 352 2197 2531 Fragments arranged by size: 2531 2197 352 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2249 2281 3721 3753 3795 Size: 32 135 32 677 32 1440 32 42 Cuts at: 3795 3827 1373 Size: 32 2626 Fragments arranged by size: 2626 1440 677 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2424 2670 669 Size: 988 281 486 246 3079 Fragments arranged by size: 3079 988 486 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5080 HpaI GTT'AAC Cuts at: 2670 2670 Size: 5080 KpnI G_GTAC'C Cuts at: 2405 2405 Size: 5080 MfeI C'AATT_G Cuts at: 2679 2679 Size: 5080 MluI A'CGCG_T Cuts at: 4956 4956 Size: 5080 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4194 4378 1305 Size: 2889 184 2007 Fragments arranged by size: 2889 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3258 Fragments arranged by size: 3258 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3038 3397 3556 518 Size: 648 1872 359 159 2042 Fragments arranged by size: 2042 1872 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2496 3173 4321 4566 1606 Size: 890 677 1148 245 2120 Fragments arranged by size: 2120 1148 890 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3589 Fragments arranged by size: 3589 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5080 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5080 NotI GC'GGCC_GC Cuts at: 2527 2527 Size: 5080 NspI r_CATG'y Cuts at: 1132 1229 2438 3983 1132 Size: 97 1209 1545 2229 Fragments arranged by size: 2229 1545 1209 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5080 PciI A'CATG_T Cuts at: 3979 3979 Size: 5080 PflMI CCAn_nnn'nTGG Cuts at: 1108 2146 1108 Size: 1038 4042 PmeI GTTT'AAAC Cuts at: 2384 2384 Size: 5080 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3149 3181 4662 4694 3149 Size: 32 1481 32 3535 Fragments arranged by size: 3535 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5080 PsiI TTA'TAA Cuts at: 2650 2650 Size: 5080 PstI C_TGCA'G Cuts at: 830 2058 2432 830 Size: 1228 374 3478 Fragments arranged by size: 3478 1228 374 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5080 SalI G'TCGA_C Cuts at: 1936 2422 1936 Size: 486 4594 SbfI CC_TGCA'GG Cuts at: 2432 2432 Size: 5080 ScaI AGT'ACT Cuts at: 3328 5040 3328 Size: 1712 3368 SfcI C'TryA_G Cuts at: 826 1045 2054 2428 3563 4244 4435 826 Size: 219 1009 374 1135 681 191 1471 Fragments arranged by size: 1471 1135 1009 681 374 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5080 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5080 SmaI CCC'GGG Cuts at: 1872 2162 2407 1872 Size: 290 245 4545 Fragments arranged by size: 4545 290 245 SmlI C'TyrA_G Cuts at: 820 1017 1943 2021 3184 4085 4347 820 Size: 197 926 78 1163 901 262 1553 Fragments arranged by size: 1553 1163 926 901 262 197 78 SnaBI TAC'GTA Cuts at: 493 2871 493 Size: 2378 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5080 SphI G_CATG'C Cuts at: 1132 2438 1132 Size: 1306 3774 SrfI GCCC'GGGC Cuts at: 2162 2162 Size: 5080 SspI AAT'ATT Cuts at: 5 52 3004 5 Size: 47 2952 2081 Fragments arranged by size: 2952 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5080 StyI C'CwwG_G Cuts at: 513 1065 2004 2519 513 Size: 552 939 515 3074 Fragments arranged by size: 3074 939 552 515 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3107 3124 3277 3462 3907 325 Size: 1255 1527 17 153 185 445 1498 Fragments arranged by size: 1527 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3326 5038 96 Size: 274 80 33 51 1443 1349 1712 138 Fragments arranged by size: 1712 1443 1349 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5040 5072 5040 Size: 32 5048 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5080 XbaI T'CTAG_A Cuts at: 2416 2416 Size: 5080 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5080 XmaI C'CCGG_G Cuts at: 1870 2160 2405 1870 Size: 290 245 4545 Fragments arranged by size: 4545 290 245 XmnI GAAnn'nnTTC Cuts at: 3209 3209 Size: 5080 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2888 276 Size: 53 83 186 936 1354 2468 Fragments arranged by size: 2468 1354 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SwaI XcmI