################################## # NO CUT ENZYMES ( 144 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BbrPI BbsI BbvCI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaWI BseAI BsiHKAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstYI BstZ17I CelII ClaI CpoI DpnII DraI DraIII DrdI Eam1105I EarI Eco47III EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI Ksp632I MaeI MaeII MaeIII MamI MboI MfeI MflI MluI MluNI MroI MscI MseI MunI NcoI NdeI NdeII NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SalI SapI Sau3AI ScaI SexAI SfiI SfuI SgrAI SmlI SnaBI SpeI SphI SspBI SspI StuI SwaI TfiI Tru9I Tsp45I Tth111I Van91I XbaI XhoI XhoII ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AcsI 321 AAATTC [GA]^AATT[CT] AocI 405 CCTGAGG CC^T[ACGT]AGG ApaI 48 GGGCCC GGGCC^C ApoI 321 AAATTC [GA]^AATT[CT] Asp700I 288 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaII 67 GGACC G^G[AT]CC BanI 88 GGCGCC G^G[CT][GA]CC BbeI 88 GGCGCC GGCGC^C BglI 91 GCCGAGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BspMI 397 ACCTGC ACCTGC(4/8) BsrBI 335 GAGCGG CCGCTC(-3/-3) BsrFI 195 GCCGGC [GA]^CCGG[CT] BstAPI 508 GCAGAGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 767 CCACGCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 405 CCTGAGG CC^T[ACGT]AGG Cfr10I 195 GCCGGC [GA]^CCGG[CT] DdeI 406 CTGAG C^T[ACGT]AG DsaI 101 CCGCGG C^C[GA][CT]GG Eco81I 405 CCTGAGG CC^T[ACGT]AGG HinfI 758 GACTC G^A[ACGT]TC HphI 285 GGTGA GGTGA(8/7) KasI 88 GGCGCC G^GCGCC KspI 101 CCGCGG CCGC^GG MboII 328 GAAGA GAAGA(8/7) MslI 768 CACGCCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 195 GCCGGC GCC^GGC NarI 88 GGCGCC GG^CGCC NgoMIV 195 GCCGGC G^CCGGC NspI 785 GCATGT [GA]CATG^[CT] PleI 758 GACTC GAGTC(4/5) PshAI 549 GACCCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacII 101 CCGCGG CCGC^GG SbfI 687 CCTGCAGG CCTGCA^GG SfoI 88 GGCGCC GGC^GCC Sse8387I 687 CCTGCAGG CCTGCA^GG TaqI 296 TCGA T^CGA Tsp509I 322 AATT ^AATT TthHB8I 296 TCGA T^CGA XcmI 743 CCATCCCGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 288 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AcyI 88 GGCGCC G[GA]^CG[CT]C 183 GGCGTC AfaI 495 GTAC GT^AC 789 GTAC BanII 48 GGGCCC G[GA]GC[CT]^C 387 GAGCCC BcgI 590 CGAAAAGACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 249 GCACGGCGCTCG BsaHI 88 GGCGCC G[GA]^CG[CT]C 183 GGCGTC BsgI 399 CTGCAC GTGCAG(16/14) 515 GTGCAG BsmFI 756 GGGAC GGGAC(10/14) 186 GTCCC BsrDI 504 CATTGC GCAATG(2/0) 20 GCAATG BsrI 612 ACTGG ACTGG(1/-1) 772 CCAGT DraII 47 AGGGCCC [GA]G^G[ACGT]CC[CT] 78 GGGGCCT EcoO109I 47 AGGGCCC [GA]G^G[ACGT]CC[CT] 78 GGGGCCT EcoT14I 176 CCAAGG C^C[AT][AT]GG 584 CCAAGG FauI 189 CCCGC CCCGC(4/6) 747 CCCGC Hin1I 88 GGCGCC G[GA]^CG[CT]C 183 GGCGTC NlaIII 156 CATG CATG^ 786 CATG PstI 598 CTGCAG CTGCA^G 688 CTGCAG RsaI 495 GTAC GT^AC 789 GTAC SfaNI 345 GATGC GCATC(5/9) 712 GCATC SfcI 598 CTGCAG C^T[GA][CT]AG 688 CTGCAG SmaI 75 CCCGGG CCC^GGG 274 CCCGGG StyI 176 CCAAGG C^C[AT][AT]GG 584 CCAAGG XmaCI 75 CCCGGG C^CCGGG 274 CCCGGG XmaI 75 CCCGGG C^CCGGG 274 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 51 enzymes) # ######################################### AccII AciI AluI AvaI BbvI BcnI BmyI BpmI BsaI BsaJI BseRI BsiEI BsiYI BslI BsmAI BsoBI Bsp1286I BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I EaeI EagI EclXI Eco52I EcoRII Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HpaII ItaI MnlI MspA1I MspI MvaI MvnI MwoI NciI NlaIV Sau96I ScrFI TseI TspRI