(Circular) (Six-base) MAPSORT of: pFN21AB8921.seq Check: 2741 from: 1 to: 5506 pFN21AB8921 5506 bp With 182 enzymes: SgfI * August 16, 2010 14:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2411 2411 Size: 5506 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3316 278 Size: 53 83 186 936 1780 2468 Fragments arranged by size: 2468 1780 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2849 1937 Size: 912 4594 Acc65I G'GTAC_C Cuts at: 2827 2827 Size: 5506 AclI AA'CG_TT Cuts at: 1563 3633 4006 1563 Size: 2070 373 3063 Fragments arranged by size: 3063 2070 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2128 2647 3569 4953 5221 1632 Size: 496 519 922 1384 268 1917 Fragments arranged by size: 1917 1384 922 519 496 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5309 AflIII A'CryG_T Cuts at: 1203 4405 5382 1203 Size: 3202 977 1327 Fragments arranged by size: 3202 1327 977 AgeI A'CCGG_T Cuts at: 4337 4337 Size: 5506 AhdI GACnn_n'nnGTC Cuts at: 4235 4235 Size: 5506 AleI CACnn'nnGTG Cuts at: 1166 2777 1166 Size: 1611 3895 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5506 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2468 2500 2468 Size: 32 5474 AlwNI CAG_nnn'CTG Cuts at: 3249 4821 3249 Size: 1572 3934 ApaI G_GGCC'C Cuts at: 2057 2057 Size: 5506 ApaLI G'TGCA_C Cuts at: 3563 4719 3563 Size: 1156 4350 ApoI r'AATT_y Cuts at: 1493 2326 2815 3041 3193 5302 1493 Size: 833 489 226 152 2109 1697 Fragments arranged by size: 2109 1697 833 489 226 152 AseI AT'TA_AT Cuts at: 160 1032 4060 160 Size: 872 3028 1606 Fragments arranged by size: 3028 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5506 AvaI C'yCGr_G Cuts at: 1870 1943 2080 2262 2279 2831 1870 Size: 73 137 182 17 552 4545 Fragments arranged by size: 4545 552 182 137 73 17 BamHI G'GATC_C Cuts at: 2836 3203 2836 Size: 367 5139 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2093 2827 4282 618 Size: 325 217 627 306 734 1455 1842 Fragments arranged by size: 1842 1455 734 627 325 306 217 BanII G_rGCy'C Cuts at: 1391 2057 2396 2825 1391 Size: 666 339 429 4072 Fragments arranged by size: 4072 666 429 339 BbeI G_GCGC'C Cuts at: 2097 2097 Size: 5506 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4535 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2243 2277 2584 2618 3697 3731 1903 Size: 34 306 34 307 34 1079 34 3678 Fragments arranged by size: 3678 1079 307 306 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3398 4608 3398 Size: 1210 4296 BclI T'GATC_A Cuts at: 1548 1548 Size: 5506 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2102 4117 136 Size: 107 122 71 631 605 430 2015 1525 Fragments arranged by size: 2015 1525 631 605 430 122 107 71 BglII A'GATC_T Cuts at: 5501 5501 Size: 5506 BlpI GC'TnA_GC Cuts at: 2923 2923 Size: 5506 Bme1580I G_kGCm'C Cuts at: 1790 2057 2256 3567 4723 1790 Size: 267 199 1311 1156 2573 Fragments arranged by size: 2573 1311 1156 267 199 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5506 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3237 4195 442 Size: 416 53 83 399 24 1820 958 1753 Fragments arranged by size: 1820 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5506 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2326 2326 Size: 5506 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2116 2495 2515 2641 4166 1239 Size: 877 379 20 126 1525 2579 Fragments arranged by size: 2579 1525 877 379 126 20 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3631 4496 4794 3631 Size: 865 298 4343 Fragments arranged by size: 4343 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2134 2297 2546 4169 882 Size: 617 635 163 249 1623 2219 Fragments arranged by size: 2219 1623 635 617 249 163 BsaAI yAC'GTr Cuts at: 493 1204 3297 493 Size: 711 2093 2702 Fragments arranged by size: 2702 2093 711 BsaBI GATnn'nnATC Cuts at: 5500 5500 Size: 5506 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2094 2189 3313 3695 Size: 53 83 186 936 561 95 1124 382 Cuts at: 3695 4330 275 Size: 635 1451 Fragments arranged by size: 1451 1124 936 635 561 382 186 95 83 53 BsaWI w'CCGG_w Cuts at: 3939 4337 4611 4758 5169 3939 Size: 398 274 147 411 4276 Fragments arranged by size: 4276 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2468 2498 1178 Size: 30 165 30 1065 30 4186 Fragments arranged by size: 4186 1065 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2063 2126 2456 1186 Size: 877 63 330 4236 Fragments arranged by size: 4236 877 330 63 BseYI C'CCAG_C Cuts at: 2056 2460 4709 5205 2056 Size: 404 2249 496 2357 Fragments arranged by size: 2357 2249 496 404 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2389 2541 1163 Size: 573 653 152 4128 Fragments arranged by size: 4128 653 573 152 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2205 2220 2365 2956 3717 4745 Size: 275 186 204 15 145 591 761 1028 Cuts at: 4745 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 591 275 204 186 145 15 BsiHKAI G_wGCw'C Cuts at: 3567 3652 4723 3567 Size: 85 1071 4350 Fragments arranged by size: 4350 1071 85 BsmI GAATG_Cn' Cuts at: 3017 3110 3017 Size: 93 5413 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2057 2256 2396 2825 3567 3652 4723 Size: 399 267 199 140 429 742 85 1071 Cuts at: 4723 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 429 399 267 199 140 85 BspEI T'CCGG_A Cuts at: 5169 5169 Size: 5506 BspHI T'CATG_A Cuts at: 3395 5125 3395 Size: 1730 3776 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2411 2861 844 Size: 1567 450 3489 Fragments arranged by size: 3489 1567 450 BsrBI CCG'CTC Cuts at: 1394 1823 2342 3393 1394 Size: 429 519 1051 3507 Fragments arranged by size: 3507 1051 519 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2032 2508 4001 4175 65 Size: 1183 784 476 1493 174 1396 Fragments arranged by size: 1493 1396 1183 784 476 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2200 4150 4337 1700 Size: 110 390 1950 187 2869 Fragments arranged by size: 2869 1950 390 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5506 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5506 BssSI C'ACGA_G Cuts at: 3566 4578 3566 Size: 1012 4494 BstAPI GCAn_nnn'nTGC Cuts at: 794 2519 794 Size: 1725 3781 BstBI TT'CG_AA Cuts at: 4310 4310 Size: 5506 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5506 BstXI CCAn_nnnn'nTGG Cuts at: 2779 2779 Size: 5506 BstYI r'GATC_y Cuts at: 1972 2836 3203 3588 3605 5057 5143 5155 5501 Size: 864 367 385 17 1452 86 12 346 Cuts at: 5501 1972 Size: 1977 Fragments arranged by size: 1977 1452 864 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2411 2411 Size: 5506 BtgI C'CryG_G Cuts at: 513 1065 2004 2106 513 Size: 552 939 102 3913 Fragments arranged by size: 3913 939 552 102 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4767 Fragments arranged by size: 4767 683 56 BtsI GCAGTG_nn' Cuts at: 2067 2237 3022 3816 3836 2067 Size: 170 785 794 20 3737 Fragments arranged by size: 3737 794 785 170 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5506 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5471 DraI TTT'AAA Cuts at: 2810 2810 Size: 5506 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4513 809 Size: 507 3197 1802 Fragments arranged by size: 3197 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2067 2202 2217 Size: 54 1425 50 275 18 237 135 15 Cuts at: 2217 2234 2362 2953 3842 5471 8 Size: 17 128 591 889 1629 43 Fragments arranged by size: 1629 1425 889 591 275 237 135 128 54 50 43 18 17 15 EagI C'GGCC_G Cuts at: 1537 1812 2202 2217 2362 2953 1537 Size: 275 390 15 145 591 4090 Fragments arranged by size: 4090 591 390 275 145 15 EarI CTCTTCn'nnn_ Cuts at: 1353 3436 1353 Size: 2083 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4093 4467 4613 387 Size: 1064 138 2504 374 146 1280 Fragments arranged by size: 2504 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2116 2128 2495 2515 2641 2647 3569 Size: 393 484 12 367 20 126 6 922 Cuts at: 3569 4166 4953 5221 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 922 787 597 484 393 367 268 126 20 12 6 EcoO109I rG'GnC_Cy Cuts at: 2053 2084 2053 Size: 31 5475 EcoRI G'AATT_C Cuts at: 2815 3193 2815 Size: 378 5128 FspI TGC'GCA Cuts at: 4012 4012 Size: 5506 HaeII r_GCGC'y Cuts at: 1678 2097 2119 2159 2214 2262 4653 1678 Size: 419 22 40 55 48 2391 2531 Fragments arranged by size: 2531 2391 419 55 48 40 22 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4147 4179 4221 4253 1373 Size: 32 135 32 2575 32 42 32 2626 Fragments arranged by size: 2626 2575 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2850 3096 669 Size: 988 281 912 246 3079 Fragments arranged by size: 3079 988 912 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5506 HpaI GTT'AAC Cuts at: 3096 3096 Size: 5506 KasI G'GCGC_C Cuts at: 2093 2093 Size: 5506 KpnI G_GTAC'C Cuts at: 2831 2831 Size: 5506 MfeI C'AATT_G Cuts at: 3105 3105 Size: 5506 MluI A'CGCG_T Cuts at: 5382 5382 Size: 5506 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2190 2301 4620 4804 1305 Size: 885 111 2319 184 2007 Fragments arranged by size: 2319 2007 885 184 111 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3684 Fragments arranged by size: 3684 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2777 3464 3823 3982 518 Size: 648 1611 687 359 159 2042 Fragments arranged by size: 2042 1611 687 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2108 2197 2287 2922 3599 4747 4992 1606 Size: 502 89 90 635 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 635 502 245 90 89 NaeI GCC'GGC Cuts at: 2202 2202 Size: 5506 NarI GG'CG_CC Cuts at: 2094 2094 Size: 5506 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4015 Fragments arranged by size: 4015 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5506 NgoMIV G'CCGG_C Cuts at: 2200 2200 Size: 5506 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5506 NotI GC'GGCC_GC Cuts at: 2953 2953 Size: 5506 NspI r_CATG'y Cuts at: 1132 1229 2794 2864 4409 1132 Size: 97 1565 70 1545 2229 Fragments arranged by size: 2229 1565 1545 97 70 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5506 PciI A'CATG_T Cuts at: 4405 4405 Size: 5506 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5506 PmeI GTTT'AAAC Cuts at: 2810 2810 Size: 5506 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2468 2500 3575 3607 5088 5120 2468 Size: 32 1075 32 1481 32 2854 Fragments arranged by size: 2854 1481 1075 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2558 1613 Size: 945 4561 PsiI TTA'TAA Cuts at: 3076 3076 Size: 5506 PspOMI G'GGCC_C Cuts at: 2053 2053 Size: 5506 PstI C_TGCA'G Cuts at: 830 2607 2697 2858 830 Size: 1777 90 161 3478 Fragments arranged by size: 3478 1777 161 90 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5506 SacII CC_GC'GG Cuts at: 2109 2109 Size: 5506 SalI G'TCGA_C Cuts at: 1936 2848 1936 Size: 912 4594 SbfI CC_TGCA'GG Cuts at: 2697 2858 2697 Size: 161 5345 ScaI AGT'ACT Cuts at: 3754 5466 3754 Size: 1712 3794 SfcI C'TryA_G Cuts at: 826 1045 2603 2693 2854 3989 4670 4861 826 Size: 219 1558 90 161 1135 681 191 1471 Fragments arranged by size: 1558 1471 1135 681 219 191 161 90 SfoI GGC'GCC Cuts at: 2095 2095 Size: 5506 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5506 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5506 SmaI CCC'GGG Cuts at: 1872 2082 2281 2833 1872 Size: 210 199 552 4545 Fragments arranged by size: 4545 552 210 199 SmlI C'TyrA_G Cuts at: 820 1017 1943 3610 4511 4773 820 Size: 197 926 1667 901 262 1553 Fragments arranged by size: 1667 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3297 493 Size: 2804 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5506 SphI G_CATG'C Cuts at: 1132 2864 1132 Size: 1732 3774 SspI AAT'ATT Cuts at: 5 52 3430 5 Size: 47 3378 2081 Fragments arranged by size: 3378 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5506 StyI C'CwwG_G Cuts at: 513 1065 2004 2181 2589 2945 513 Size: 552 939 177 408 356 3074 Fragments arranged by size: 3074 939 552 408 356 177 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2302 3533 3550 3703 3888 4333 325 Size: 1255 722 1231 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1231 722 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3752 5464 96 Size: 274 80 33 51 1443 1775 1712 138 Fragments arranged by size: 1775 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5466 5498 5466 Size: 32 5474 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5506 XbaI T'CTAG_A Cuts at: 2842 2842 Size: 5506 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2755 2755 Size: 5506 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5506 XmaI C'CCGG_G Cuts at: 1870 2080 2279 2831 1870 Size: 210 199 552 4545 Fragments arranged by size: 4545 552 210 199 XmnI GAAnn'nnTTC Cuts at: 2297 3635 2297 Size: 1338 4168 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3314 276 Size: 53 83 186 936 1780 2468 Fragments arranged by size: 2468 1780 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI