################################## # NO CUT ENZYMES ( 147 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BamHI BbrPI BciVI BclI BfrI BglI BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HgaI HincII HindII HindIII HpaI KpnI KspI MaeIII MamI MluI MroI MseI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tru9I Tsp45I Tth111I Van91I XcmI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AcsI 655 GAATTT [GA]^AATT[CT] AcyI 167 GGCGCC G[GA]^CG[CT]C AlwI 774 GGATC GGATC(4/5) ApoI 655 GAATTT [GA]^AATT[CT] BanI 167 GGCGCC G^G[CT][GA]CC BbeI 167 GGCGCC GGCGC^C BbsI 760 GAAGAC GAAGAC(2/6) BglII 582 AGATCT A^GATCT BpuAI 760 GAAGAC GAAGAC(2/6) BsaHI 167 GGCGCC G[GA]^CG[CT]C BseRI 314 GAGGAG GAGGAG(10/8) BsmAI 245 GAGAC GTCTC(1/5) BsrDI 502 CATTGC GCAATG(2/0) BssHII 134 GCGCGC G^CGCGC BssSI 687 CACGAG CACGAG(-5/-1) BstF5I 552 GGATG GGATG(2/0) BstXI 217 CCACCCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 582 AGATCT [GA]^GATC[CT] DsaI 223 CCGTGG C^C[GA][CT]GG EarI 694 GAAGAG CTCTTC(1/4) EcoT14I 746 CCAAGG C^C[AT][AT]GG FokI 552 GGATG GGATG(9/13) Hin1I 167 GGCGCC G[GA]^CG[CT]C HphI 811 GGTGA GGTGA(8/7) KasI 167 GGCGCC G^GCGCC Ksp632I 694 GAAGAG CTCTTC(1/4) MaeII 341 ACGT A^CGT MfeI 866 CAATTG C^AATTG MflI 582 AGATCT [GA]^GATC[CT] MunI 866 CAATTG C^AATTG NarI 167 GGCGCC GG^CGCC NlaIII 879 CATG CATG^ PaeR7I 79 CTCGAG C^TCGAG SfaNI 6 GATGC GCATC(5/9) SfoI 167 GGCGCC GGC^GCC SmaI 268 CCCGGG CCC^GGG SmlI 79 CTCGAG C^T[CT][GA]AG StyI 746 CCAAGG C^C[AT][AT]GG TfiI 540 GATTC G^A[AT]TC XbaI 819 TCTAGA T^CTAGA XhoI 79 CTCGAG C^TCGAG XhoII 582 AGATCT [GA]^GATC[CT] XmaCI 268 CCCGGG C^CCGGG XmaI 268 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AfaI 19 GTAC GT^AC 792 GTAC AluI 318 AGCT AG^CT 858 AGCT ApaI 90 GGGCCC GGGCC^C 237 GGGCCC BanII 90 GGGCCC G[GA]GC[CT]^C 237 GGGCCC BbvCI 198 CCTCAGC CCTCAGC(-5/-2) 120 GCTGAGG BbvI 381 GCAGC GCAGC(8/12) 859 GCTGC BcgI 99 CGAGGGGACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 110 CGAGAGTCTTGC BmyI 90 GGGCCC G[AGT]GC[ACT]^C 237 GGGCCC Bsp1286I 90 GGGCCC G[AGT]GC[ACT]^C 237 GGGCCC DdeI 121 CTGAG C^T[ACGT]AG 199 CTCAG HaeII 59 GGCGCT [GA]GCGC^[CT] 167 GGCGCC MslI 218 CACCCCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 873 CACACACATG RsaI 19 GTAC GT^AC 792 GTAC TseI 381 GCAGC G^C[AT]GC 859 GCTGC TspRI 204 CACTG CA[GC]TG[ACGT][ACGT]^ 472 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 52 enzymes) # ######################################### AccII AciI AvaI AvaII BalI BcnI BfaI BsaJI BsiYI BslI BsmFI BsoBI BsrI BssKI BstNI BstUI Cac8I CfoI Cfr13I DpnII DraII EaeI EcoO109I EcoRII Fnu4HI HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MaeI MboI MboII MluNI MnlI MscI MspI MvaI MvnI MwoI NciI NdeII NlaIV PleI Sau3AI Sau96I ScrFI TaqI Tsp509I TthHB8I