(Circular) (Six-base) MAPSORT of: pFN21AE6221.seq Check: 9306 from: 1 to: 5608 pFN21AE6221 5608 bp With 182 enzymes: SgfI * March 18, 2015 14:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3418 278 Size: 53 83 186 936 1882 2468 Fragments arranged by size: 2468 1882 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2951 1937 Size: 1014 4594 Acc65I G'GTAC_C Cuts at: 2929 2929 Size: 5608 AclI AA'CG_TT Cuts at: 1563 3735 4108 1563 Size: 2172 373 3063 Fragments arranged by size: 3063 2172 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3671 5055 5323 1632 Size: 2039 1384 268 1917 Fragments arranged by size: 2039 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5411 AflIII A'CryG_T Cuts at: 1203 4507 5484 1203 Size: 3304 977 1327 Fragments arranged by size: 3304 1327 977 AgeI A'CCGG_T Cuts at: 4439 4439 Size: 5608 AhdI GACnn_n'nnGTC Cuts at: 4337 4337 Size: 5608 AleI CACnn'nnGTG Cuts at: 1166 2227 1166 Size: 1061 4547 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5608 AlwNI CAG_nnn'CTG Cuts at: 3351 4923 3351 Size: 1572 4036 ApaI G_GGCC'C Cuts at: 2099 2246 2099 Size: 147 5461 ApaLI G'TGCA_C Cuts at: 3665 4821 3665 Size: 1156 4452 ApoI r'AATT_y Cuts at: 1493 2660 2917 3143 3295 5404 1493 Size: 1167 257 226 152 2109 1697 Fragments arranged by size: 2109 1697 1167 257 226 152 AseI AT'TA_AT Cuts at: 160 1032 4162 160 Size: 872 3130 1606 Fragments arranged by size: 3130 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5608 AvaI C'yCGr_G Cuts at: 1870 1943 2084 2167 2273 2933 1870 Size: 73 141 83 106 660 4545 Fragments arranged by size: 4545 660 141 106 83 73 BamHI G'GATC_C Cuts at: 2938 3305 2938 Size: 367 5241 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2172 2929 4384 618 Size: 325 217 627 385 757 1455 1842 Fragments arranged by size: 1842 1455 757 627 385 325 217 BanII G_rGCy'C Cuts at: 1391 2099 2246 2927 1391 Size: 708 147 681 4072 Fragments arranged by size: 4072 708 681 147 BbeI G_GCGC'C Cuts at: 2176 2176 Size: 5608 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2772 928 Size: 971 873 3764 Fragments arranged by size: 3764 971 873 BbvCI CC'TCA_GC Cuts at: 2126 2204 2126 Size: 78 5530 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2093 2104 2127 2138 3799 3833 1903 Size: 34 156 11 23 11 1661 34 3678 Fragments arranged by size: 3678 1661 156 34 34 23 11 11 BciVI GTATCCnnnnn_n' Cuts at: 3500 4710 3500 Size: 1210 4398 BclI T'GATC_A Cuts at: 1548 1548 Size: 5608 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4219 136 Size: 107 122 71 631 605 2547 1525 Fragments arranged by size: 2547 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2587 5603 2587 Size: 3016 2592 BlpI GC'TnA_GC Cuts at: 3025 3025 Size: 5608 Bme1580I G_kGCm'C Cuts at: 1790 2099 2246 3669 4825 1790 Size: 309 147 1423 1156 2573 Fragments arranged by size: 2573 1423 1156 309 147 BmgBI CAC'GTC Cuts at: 1318 2347 1318 Size: 1029 4579 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2451 3339 4297 Size: 416 53 83 399 24 1034 888 958 Cuts at: 4297 442 Size: 1753 Fragments arranged by size: 1753 1034 958 888 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5608 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4268 1239 Size: 3029 2579 Bpu10I CC'TnA_GC Cuts at: 2126 2204 2126 Size: 78 5530 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3733 4598 4896 3733 Size: 865 298 4445 Fragments arranged by size: 4445 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4271 882 Size: 617 2772 2219 Fragments arranged by size: 2772 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3399 493 Size: 711 2195 2702 Fragments arranged by size: 2702 2195 711 BsaBI GATnn'nnATC Cuts at: 5602 5602 Size: 5608 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2173 3415 3797 4432 Size: 53 83 186 936 640 1242 382 635 Cuts at: 4432 275 Size: 1451 Fragments arranged by size: 1451 1242 936 640 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4041 4439 4713 4860 5271 4041 Size: 398 274 147 411 4378 Fragments arranged by size: 4378 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5383 Fragments arranged by size: 5383 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2334 1186 Size: 1148 4460 BseYI C'CCAG_C Cuts at: 2098 4811 5307 2098 Size: 2713 496 2399 Fragments arranged by size: 2713 2399 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5035 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3058 3819 4847 1540 Size: 275 186 1057 761 1028 2301 Fragments arranged by size: 2301 1057 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3669 3754 4825 3669 Size: 85 1071 4452 Fragments arranged by size: 4452 1071 85 BsmI GAATG_Cn' Cuts at: 3119 3212 3119 Size: 93 5515 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2099 2246 2927 3669 3754 4825 1391 Size: 399 309 147 681 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 681 399 309 147 85 BspEI T'CCGG_A Cuts at: 5271 5271 Size: 5608 BspHI T'CATG_A Cuts at: 3497 5227 3497 Size: 1730 3878 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2963 844 Size: 2119 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3495 1394 Size: 429 1672 3507 Fragments arranged by size: 3507 1672 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2506 4103 4277 65 Size: 1183 1258 1597 174 1396 Fragments arranged by size: 1597 1396 1258 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4252 4439 1700 Size: 110 2442 187 2869 Fragments arranged by size: 2869 2442 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5608 BssHII G'CGCG_C Cuts at: 1925 2139 1925 Size: 214 5394 BssSI C'ACGA_G Cuts at: 2692 3668 4680 2692 Size: 976 1012 3620 Fragments arranged by size: 3620 1012 976 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5608 BstBI TT'CG_AA Cuts at: 4412 4412 Size: 5608 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5608 BstXI CCAn_nnnn'nTGG Cuts at: 2229 2229 Size: 5608 BstYI r'GATC_y Cuts at: 1972 2587 2938 3305 3690 3707 5159 5245 5257 Size: 615 351 367 385 17 1452 86 12 Cuts at: 5257 5603 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 615 385 367 351 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2228 513 Size: 552 939 224 3893 Fragments arranged by size: 3893 939 552 224 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2024 2626 487 Size: 683 56 798 602 3469 Fragments arranged by size: 3469 798 683 602 56 BtsI GCAGTG_nn' Cuts at: 2208 3124 3918 3938 2208 Size: 916 794 20 3878 Fragments arranged by size: 3878 916 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5608 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5573 DraI TTT'AAA Cuts at: 2912 2912 Size: 5608 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4615 809 Size: 507 3299 1802 Fragments arranged by size: 3299 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2053 2715 2836 Size: 54 1425 50 275 18 223 662 121 Cuts at: 2836 3055 3944 5573 8 Size: 219 889 1629 43 Fragments arranged by size: 1629 1425 889 662 275 223 219 121 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3055 1537 Size: 275 1243 4090 Fragments arranged by size: 4090 1243 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2694 3538 1353 Size: 1341 844 3423 Fragments arranged by size: 3423 1341 844 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4195 4569 4715 387 Size: 1064 138 2606 374 146 1280 Fragments arranged by size: 2606 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3671 4268 5055 5323 1239 Size: 393 2039 597 787 268 1524 Fragments arranged by size: 2039 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2095 2242 2243 2095 Size: 147 1 5460 Fragments arranged by size: 5460 147 1 EcoRI G'AATT_C Cuts at: 2917 3295 2917 Size: 378 5230 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2485 2485 Size: 5608 FspI TGC'GCA Cuts at: 4114 4114 Size: 5608 HaeII r_GCGC'y Cuts at: 1678 2068 2176 4755 1678 Size: 390 108 2579 2531 Fragments arranged by size: 2579 2531 390 108 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2688 2720 4249 4281 4323 Size: 32 135 32 1116 32 1529 32 42 Cuts at: 4323 4355 1373 Size: 32 2626 Fragments arranged by size: 2626 1529 1116 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2952 3198 669 Size: 988 281 1014 246 3079 Fragments arranged by size: 3079 1014 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5608 HpaI GTT'AAC Cuts at: 3198 3198 Size: 5608 KasI G'GCGC_C Cuts at: 2172 2172 Size: 5608 KpnI G_GTAC'C Cuts at: 2933 2933 Size: 5608 MfeI C'AATT_G Cuts at: 2871 3207 2871 Size: 336 5272 MluI A'CGCG_T Cuts at: 5484 5484 Size: 5608 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4722 4906 1305 Size: 3417 184 2007 Fragments arranged by size: 3417 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2055 2717 2838 10 Size: 54 1425 343 223 662 121 2780 Fragments arranged by size: 2780 1425 662 343 223 121 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2227 2882 3566 3925 4084 518 Size: 648 843 218 655 684 359 159 2042 Fragments arranged by size: 2042 843 684 655 648 359 218 159 MspA1I CmG'CkG Cuts at: 1606 3024 3701 4849 5094 1606 Size: 1418 677 1148 245 2120 Fragments arranged by size: 2120 1418 1148 677 245 NarI GG'CG_CC Cuts at: 2173 2173 Size: 5608 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4117 Fragments arranged by size: 4117 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5608 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5608 NotI GC'GGCC_GC Cuts at: 3055 3055 Size: 5608 NspI r_CATG'y Cuts at: 1132 1229 2966 4511 1132 Size: 97 1737 1545 2229 Fragments arranged by size: 2229 1737 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5608 PciI A'CATG_T Cuts at: 4507 4507 Size: 5608 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5608 PfoI T'CCnGG_A Cuts at: 2285 2285 Size: 5608 PmeI GTTT'AAAC Cuts at: 2912 2912 Size: 5608 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3677 3709 5190 5222 3677 Size: 32 1481 32 4063 Fragments arranged by size: 4063 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5608 PsiI TTA'TAA Cuts at: 3178 3178 Size: 5608 PspOMI G'GGCC_C Cuts at: 2095 2242 2095 Size: 147 5461 PspXI vC'TCGA_Gb Cuts at: 2084 2084 Size: 5608 PstI C_TGCA'G Cuts at: 830 2960 830 Size: 2130 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5608 SalI G'TCGA_C Cuts at: 1936 2950 1936 Size: 1014 4594 SbfI CC_TGCA'GG Cuts at: 2960 2960 Size: 5608 ScaI AGT'ACT Cuts at: 3856 5568 3856 Size: 1712 3896 SfcI C'TryA_G Cuts at: 826 1045 2956 4091 4772 4963 826 Size: 219 1911 1135 681 191 1471 Fragments arranged by size: 1911 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2174 2174 Size: 5608 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5608 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5608 SmaI CCC'GGG Cuts at: 1872 2275 2935 1872 Size: 403 660 4545 Fragments arranged by size: 4545 660 403 SmlI C'TyrA_G Cuts at: 820 1017 1943 2084 3712 4613 4875 820 Size: 197 926 141 1628 901 262 1553 Fragments arranged by size: 1628 1553 926 901 262 197 141 SnaBI TAC'GTA Cuts at: 493 3399 493 Size: 2906 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5608 SphI G_CATG'C Cuts at: 1132 2966 1132 Size: 1834 3774 SrfI GCCC'GGGC Cuts at: 2275 2275 Size: 5608 SspI AAT'ATT Cuts at: 5 52 3532 5 Size: 47 3480 2081 Fragments arranged by size: 3480 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5608 StyI C'CwwG_G Cuts at: 513 1065 2004 2751 3047 513 Size: 552 939 747 296 3074 Fragments arranged by size: 3074 939 747 552 296 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2039 3635 3652 3805 3990 4435 325 Size: 1255 459 1596 17 153 185 445 1498 Fragments arranged by size: 1596 1498 1255 459 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2796 3854 5566 Size: 274 80 33 51 1443 819 1058 1712 Cuts at: 5566 96 Size: 138 Fragments arranged by size: 1712 1443 1058 819 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5568 5600 5568 Size: 32 5576 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5608 XbaI T'CTAG_A Cuts at: 2824 2944 2824 Size: 120 5488 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 5608 XhoI C'TCGA_G Cuts at: 1943 2084 1943 Size: 141 5467 XmaI C'CCGG_G Cuts at: 1870 2273 2933 1870 Size: 403 660 4545 Fragments arranged by size: 4545 660 403 XmnI GAAnn'nnTTC Cuts at: 3737 3737 Size: 5608 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3416 276 Size: 53 83 186 936 1882 2468 Fragments arranged by size: 2468 1882 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PspXI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SwaI