################################## # NO CUT ENZYMES ( 147 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AvaI AviII AvrII BbeI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstUI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DdeI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRV EcoT22I FauI FbaI FseI FspI HaeII HgaI Hin1I HpaI KasI KpnI KspI MaeI MamI MluI MroI MseI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PaeR7I PflMI PinAI PleI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tru9I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AcsI 853 GAATTC [GA]^AATT[CT] AfaI 549 GTAC GT^AC AlwNI 356 CAGTGTCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 853 GAATTC [GA]^AATT[CT] AspI 712 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 658 GGATCC G^GATCC BanI 64 GGCACC G^G[CT][GA]CC BanII 541 GGGCTC G[GA]GC[CT]^C BbrPI 438 CACGTG CAC^GTG BbsI 229 GAAGAC GAAGAC(2/6) BcnI 696 CCCGG CC^[GC]GG BpmI 517 CTGGAG CTGGAG(16/14) BpuAI 229 GAAGAC GAAGAC(2/6) BsaAI 438 CACGTG [CT]AC^GT[GA] BsgI 841 GTGCAG GTGCAG(16/14) BstBI 6 TTCGAA TT^CGAA BstXI 110 CCACCAAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraII 710 AGGACCC [GA]G^G[ACGT]CC[CT] EarI 222 GAAGAG CTCTTC(1/4) EcoNI 293 CCTTGGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 710 AGGACCC [GA]G^G[ACGT]CC[CT] EcoRI 853 GAATTC G^AATTC HapII 697 CCGG C^CGG HpaII 697 CCGG C^CGG HphI 373 GGTGA GGTGA(8/7) Ksp632I 222 GAAGAG CTCTTC(1/4) MslI 486 CATCGGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 514 CAGCTG C[AC]G^C[GT]G MspI 697 CCGG C^CGG NciI 696 CCCGG CC^[GC]GG NcoI 676 CCATGG C^CATGG NspV 6 TTCGAA TT^CGAA PflFI 712 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 438 CACGTG CAC^GTG PmlI 438 CACGTG CAC^GTG PpuMI 710 AGGACCC [GA]G^G[AT]CC[CT] PstI 77 CTGCAG CTGCA^G PvuII 514 CAGCTG CAG^CTG RsaI 549 GTAC GT^AC SexAI 566 ACCAGGT A^CC[AT]GGT SfuI 6 TTCGAA TT^CGAA SmlI 544 CTCAAG C^T[CT][GA]AG Tth111I 712 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 777 CCAGCTACAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AciI 138 CCGC CCGC(-3/-1) 507 CCGC AspHI 650 GTGCTC G[AT]GC[AT]^C 700 GTGCTC BbvI 513 GCAGC GCAGC(8/12) 822 GCAGC BseRI 172 GAGGAG GAGGAG(10/8) 871 GAGGAG BsiHKAI 650 GTGCTC G[AT]GC[AT]^C 700 GTGCTC BsiYI 49 CCTGTCCTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 293 CCTTGGAAAGG BslI 49 CCTGTCCTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 293 CCTTGGAAAGG BspMI 177 GCAGGT ACCTGC(4/8) 843 GCAGGT BstF5I 192 CATCC GGATG(2/0) 580 CATCC BstYI 426 GGATCT [GA]^GATC[CT] 658 GGATCC DsaI 449 CCGTGG C^C[GA][CT]GG 676 CCATGG Fnu4HI 513 GCAGC GC^[ACGT]GC 822 GCAGC FokI 192 CATCC GGATG(9/13) 580 CATCC HincII 331 GTTGAC GT[CT]^[GA]AC 861 GTTGAC HindII 331 GTTGAC GT[CT]^[GA]AC 861 GTTGAC HindIII 98 AAGCTT A^AGCTT 250 AAGCTT HinfI 4 GATTC G^A[ACGT]TC 148 GATTC ItaI 513 GCAGC GC^[ACGT]GC 822 GCAGC MaeII 439 ACGT A^CGT 839 ACGT MaeIII 38 GTCAC ^GT[ACGT]AC 303 GTCAC MfeI 122 CAATTG C^AATTG 319 CAATTG MflI 426 GGATCT [GA]^GATC[CT] 658 GGATCC MunI 122 CAATTG C^AATTG 319 CAATTG NlaIII 45 CATG CATG^ 677 CATG SfaNI 466 GATGC GCATC(5/9) 827 GCATC TaqI 7 TCGA T^CGA 270 TCGA TfiI 4 GATTC G^A[AT]TC 148 GATTC TseI 513 GCAGC G^C[AT]GC 822 GCAGC Tsp45I 38 GTCAC ^GT[GC]AC 303 GTCAC TthHB8I 7 TCGA T^CGA 270 TCGA XhoII 426 GGATCT [GA]^GATC[CT] 658 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 36 enzymes) # ######################################### AluI AlwI AvaII BalI BmyI BsaJI Bsp1286I BsrI BssKI BstNI Cac8I CfoI Cfr13I DpnII EaeI EcoRII EcoT14I HaeIII HhaI HinP1I MboI MboII MluNI MnlI MscI MvaI MwoI NdeII NlaIV Sau3AI Sau96I ScrFI SfcI StyI Tsp509I TspRI