################################## # NO CUT ENZYMES ( 137 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AfaI AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI ApoI AscI Asp718I AspEI AspI AvaI AviII AvrII BalI BbrPI BbvCI BcgI BciVI BclI BglII BlnI BlpI Bpu1102I BsaAI BsaI BsiEI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspHI BsrBI BsrDI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I CelII ClaI CpoI DraI DraII DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HpaI HphI KpnI MaeII MaeIII MluI MluNI MscI MslI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI PvuII RcaI RsaI RsrII SacI SalI SapI SbfI ScaI SexAI SfiI SfuI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tsp45I TspRI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 67 enzymes) # ################################### AccII 39 CGCG CG^CG AccIII 46 TCCGGA T^CCGGA AflII 697 CTTAAG C^TTAAG AflIII 639 ACATGT A^C[GA][CT]GT AocI 129 CCTCAGG CC^T[ACGT]AGG AseI 414 ATTAAT AT^TAAT AsnI 414 ATTAAT AT^TAAT Asp700I 703 GAAATTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaII 111 GGACC G^G[AT]CC BamHI 687 GGATCC G^GATCC BanI 88 GGCGCC G^G[CT][GA]CC BanII 34 GAGCCC G[GA]GC[CT]^C BbeI 88 GGCGCC GGCGC^C BbsI 247 GAAGAC GAAGAC(2/6) BcnI 60 CCGGG CC^[GC]GG BfaI 368 CTAG C^TAG BfrI 697 CTTAAG C^TTAAG BpuAI 247 GAAGAC GAAGAC(2/6) BsaBI 582 GATAAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 46 TCCGGA [AT]^CCGG[AT] BseAI 46 TCCGGA T^CCGGA BsmAI 318 GAGAC GTCTC(1/5) BspEI 46 TCCGGA T^CCGGA BspLU11I 639 ACATGT A^CATGT BspMI 78 GCAGGT ACCTGC(4/8) BsrFI 85 GCCGGC [GA]^CCGG[CT] BstF5I 314 GGATG GGATG(2/0) BstUI 39 CGCG CG^CG BstYI 687 GGATCC [GA]^GATC[CT] Bsu36I 129 CCTCAGG CC^T[ACGT]AGG CfoI 89 GCGC GCG^C Cfr10I 85 GCCGGC [GA]^CCGG[CT] DsaI 38 CCGCGG C^C[GA][CT]GG Eco81I 129 CCTCAGG CC^T[ACGT]AGG FokI 314 GGATG GGATG(9/13) HaeII 88 GGCGCC [GA]GCGC^[CT] HgaI 25 GACGC GACGC(5/10) HhaI 89 GCGC GCG^C HinP1I 89 GCGC G^CGC HincII 730 GTTGAC GT[CT]^[GA]AC HindII 730 GTTGAC GT[CT]^[GA]AC KasI 88 GGCGCC G^GCGCC KspI 38 CCGCGG CCGC^GG MaeI 368 CTAG C^TAG MamI 582 GATAAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 675 CAATTG C^AATTG MflI 687 GGATCC [GA]^GATC[CT] MroI 46 TCCGGA T^CCGGA MspA1I 38 CCGCGG C[AC]G^C[GT]G MunI 675 CAATTG C^AATTG MvnI 39 CGCG CG^CG NaeI 85 GCCGGC GCC^GGC NarI 88 GGCGCC GG^CGCC NciI 60 CCGGG CC^[GC]GG NgoMIV 85 GCCGGC G^CCGGC NspI 639 ACATGT [GA]CATG^[CT] PleI 43 GACTC GAGTC(4/5) PshBI 414 ATTAAT AT^TAAT PstI 712 CTGCAG CTGCA^G SacII 38 CCGCGG CCGC^GG SfoI 88 GGCGCC GGC^GCC SgrAI 84 CGCCGGCG C[GA]^CCGG[CT]G TaqI 233 TCGA T^CGA TfiI 436 GAATC G^A[AT]TC TthHB8I 233 TCGA T^CGA XhoII 687 GGATCC [GA]^GATC[CT] XmnI 703 GAAATTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AcyI 25 GACGCC G[GA]^CG[CT]C 88 GGCGCC AlwI 688 GATCC GGATC(4/5) 687 GGATC AspHI 140 GAGCAC G[AT]GC[AT]^C 373 GTGCTC BglI 59 GCCGGGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 91 GCCCAGGCGGC BpmI 203 CTCCAG CTGGAG(16/14) 346 CTGGAG BsaHI 25 GACGCC G[GA]^CG[CT]C 88 GGCGCC BsaJI 38 CCGCGG C^C[ACGT][ACGT]GG 92 CCCAGG BseRI 127 CTCCTC GAGGAG(10/8) 4 GAGGAG BsgI 329 CTGCAC GTGCAG(16/14) 102 GTGCAG BsiHKAI 140 GAGCAC G[AT]GC[AT]^C 373 GTGCTC BsiYI 603 CCTACAGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 654 CCTTATTTTGG BslI 603 CCTACAGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 654 CCTTATTTTGG Cfr13I 70 GGGCC G^G[ACGT]CC 111 GGACC DpnII 588 GATC ^GATC 688 GATC EarI 268 GAAGAG CTCTTC(1/4) 477 GAAGAG FauI 37 CCCGC CCCGC(4/6) 68 GCGGG HaeIII 58 GGCC GG^CC 71 GGCC Hin1I 25 GACGCC G[GA]^CG[CT]C 88 GGCGCC HinfI 43 GACTC G^A[ACGT]TC 436 GAATC Ksp632I 268 GAAGAG CTCTTC(1/4) 477 GAAGAG MboI 588 GATC ^GATC 688 GATC NdeII 588 GATC ^GATC 688 GATC NlaIV 88 GGCGCC GG[ACGT]^[ACGT]CC 687 GGATCC Sau3AI 588 GATC ^GATC 688 GATC Sau96I 70 GGGCC G^G[ACGT]CC 111 GGACC SfaNI 18 GATGC GCATC(5/9) 184 GATGC SfcI 604 CTACAG C^T[GA][CT]AG 712 CTGCAG SmlI 697 CTTAAG C^T[CT][GA]AG 718 CTTGAG ######################################### # 3 OR MORE CUT ENZYMES ( 26 enzymes) # ######################################### AciI AluI BbvI BmyI Bsp1286I BssKI BstNI Cac8I DdeI EcoRII Fnu4HI HapII HindIII HpaII ItaI MboII MnlI MseI MspI MvaI MwoI NlaIII ScrFI Tru9I TseI Tsp509I