(Circular) (Six-base) MAPSORT of: pFN21AE9660.seq Check: 5185 from: 1 to: 5446 pFN21AE9660 5446 bp With 182 enzymes: SgfI * December 28, 2011 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3256 278 Size: 53 83 186 936 1720 2468 Fragments arranged by size: 2468 1720 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2789 1937 Size: 852 4594 Acc65I G'GTAC_C Cuts at: 2767 2767 Size: 5446 AclI AA'CG_TT Cuts at: 1563 3573 3946 1563 Size: 2010 373 3063 Fragments arranged by size: 3063 2010 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2490 3509 4893 5161 1632 Size: 858 1019 1384 268 1917 Fragments arranged by size: 1917 1384 1019 858 268 AflII C'TTAA_G Cuts at: 820 1017 2702 820 Size: 197 1685 3564 Fragments arranged by size: 3564 1685 197 AflIII A'CryG_T Cuts at: 1203 2644 4345 5322 1203 Size: 1441 1701 977 1327 Fragments arranged by size: 1701 1441 1327 977 AgeI A'CCGG_T Cuts at: 4277 4277 Size: 5446 AhdI GACnn_n'nnGTC Cuts at: 4175 4175 Size: 5446 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5446 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2144 1184 Size: 960 4486 AlwNI CAG_nnn'CTG Cuts at: 3189 4761 3189 Size: 1572 3874 ApaLI G'TGCA_C Cuts at: 3503 4659 3503 Size: 1156 4290 ApoI r'AATT_y Cuts at: 1493 2755 2981 3133 5242 1493 Size: 1262 226 152 2109 1697 Fragments arranged by size: 2109 1697 1262 226 152 AseI AT'TA_AT Cuts at: 160 1032 2420 4000 160 Size: 872 1388 1580 1606 Fragments arranged by size: 1606 1580 1388 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5446 AvaI C'yCGr_G Cuts at: 1870 1943 2771 1870 Size: 73 828 4545 Fragments arranged by size: 4545 828 73 BamHI G'GATC_C Cuts at: 2692 2776 3143 2692 Size: 84 367 4995 Fragments arranged by size: 4995 367 84 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2093 2767 4222 618 Size: 325 217 627 306 674 1455 1842 Fragments arranged by size: 1842 1455 674 627 325 306 217 BanII G_rGCy'C Cuts at: 1391 2043 2765 1391 Size: 652 722 4072 Fragments arranged by size: 4072 722 652 BbeI G_GCGC'C Cuts at: 2097 2097 Size: 5446 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2259 928 Size: 971 360 4115 Fragments arranged by size: 4115 971 360 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3637 3671 1903 Size: 34 1700 34 3678 Fragments arranged by size: 3678 1700 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3338 4548 3338 Size: 1210 4236 BclI T'GATC_A Cuts at: 1548 1548 Size: 5446 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2070 2102 4057 Size: 107 122 71 631 605 398 32 1955 Cuts at: 4057 136 Size: 1525 Fragments arranged by size: 1955 1525 631 605 398 122 107 71 32 BglII A'GATC_T Cuts at: 5441 5441 Size: 5446 BlpI GC'TnA_GC Cuts at: 2863 2863 Size: 5446 Bme1580I G_kGCm'C Cuts at: 1790 3507 4663 1790 Size: 1717 1156 2573 Fragments arranged by size: 2573 1717 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5446 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3177 4135 442 Size: 416 53 83 399 24 1760 958 1753 Fragments arranged by size: 1760 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5446 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2193 2372 4106 1239 Size: 954 179 1734 2579 Fragments arranged by size: 2579 1734 954 179 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2744 3571 4436 4734 2744 Size: 827 865 298 3456 Fragments arranged by size: 3456 865 827 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4109 882 Size: 617 2610 2219 Fragments arranged by size: 2610 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3237 493 Size: 711 2033 2702 Fragments arranged by size: 2702 2033 711 BsaBI GATnn'nnATC Cuts at: 2591 5440 2591 Size: 2849 2597 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2031 2094 3253 3635 Size: 53 83 186 936 498 63 1159 382 Cuts at: 3635 4270 275 Size: 635 1451 Fragments arranged by size: 1451 1159 936 635 498 382 186 83 63 53 BsaWI w'CCGG_w Cuts at: 2051 3879 4277 4551 4698 5109 2051 Size: 1828 398 274 147 411 2388 Fragments arranged by size: 2388 1828 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5221 Fragments arranged by size: 5221 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2024 2123 1186 Size: 838 99 4509 Fragments arranged by size: 4509 838 99 BseYI C'CCAG_C Cuts at: 4649 5145 4649 Size: 496 4950 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2128 2319 1163 Size: 573 392 191 4290 Fragments arranged by size: 4290 573 392 191 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2896 3657 4685 1540 Size: 275 186 895 761 1028 2301 Fragments arranged by size: 2301 1028 895 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2149 2382 3507 3592 4663 2149 Size: 233 1125 85 1071 2932 Fragments arranged by size: 2932 1125 1071 233 85 BsmI GAATG_Cn' Cuts at: 2957 3050 2957 Size: 93 5353 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2043 2149 2382 2765 3507 3592 4663 Size: 399 253 106 233 383 742 85 1071 Cuts at: 4663 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 383 253 233 106 85 BspEI T'CCGG_A Cuts at: 2051 5109 2051 Size: 3058 2388 BspHI T'CATG_A Cuts at: 3335 5065 3335 Size: 1730 3716 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2074 2801 844 Size: 1230 727 3489 Fragments arranged by size: 3489 1230 727 BsrBI CCG'CTC Cuts at: 1394 1823 3333 1394 Size: 429 1510 3507 Fragments arranged by size: 3507 1510 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3941 4115 65 Size: 1183 2693 174 1396 Fragments arranged by size: 2693 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2090 4090 4277 1700 Size: 110 280 2000 187 2869 Fragments arranged by size: 2869 2000 280 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5446 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5446 BssSI C'ACGA_G Cuts at: 3506 4518 3506 Size: 1012 4434 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5446 BstBI TT'CG_AA Cuts at: 4250 4250 Size: 5446 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5446 BstYI r'GATC_y Cuts at: 1972 2692 2776 3143 3528 3545 4997 5083 5095 Size: 720 84 367 385 17 1452 86 12 Cuts at: 5095 5441 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 720 385 367 346 86 84 17 12 Bsu36I CC'TnA_GG Cuts at: 2135 2135 Size: 5446 BtgI C'CryG_G Cuts at: 513 1065 2004 2043 513 Size: 552 939 39 3916 Fragments arranged by size: 3916 939 552 39 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4707 Fragments arranged by size: 4707 683 56 BtsI GCAGTG_nn' Cuts at: 2962 3756 3776 2962 Size: 794 20 4632 Fragments arranged by size: 4632 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5446 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5411 DraI TTT'AAA Cuts at: 2750 2750 Size: 5446 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4453 809 Size: 507 3137 1802 Fragments arranged by size: 3137 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2893 3782 5411 Size: 54 1425 50 275 18 1063 889 1629 Cuts at: 5411 8 Size: 43 Fragments arranged by size: 1629 1425 1063 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2893 1537 Size: 275 1081 4090 Fragments arranged by size: 4090 1081 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2268 2477 3376 1353 Size: 915 209 899 3423 Fragments arranged by size: 3423 915 899 209 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4033 4407 4553 387 Size: 1064 138 2444 374 146 1280 Fragments arranged by size: 2444 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2193 2372 2490 3509 4106 4893 5161 Size: 393 561 179 118 1019 597 787 268 Cuts at: 5161 1239 Size: 1524 Fragments arranged by size: 1524 1019 787 597 561 393 268 179 118 EcoRI G'AATT_C Cuts at: 2755 3133 2755 Size: 378 5068 FspI TGC'GCA Cuts at: 3952 3952 Size: 5446 HaeII r_GCGC'y Cuts at: 1678 2097 4593 1678 Size: 419 2496 2531 Fragments arranged by size: 2531 2496 419 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4087 4119 4161 4193 1373 Size: 32 135 32 2515 32 42 32 2626 Fragments arranged by size: 2626 2515 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2737 2790 3036 669 Size: 988 281 799 53 246 3079 Fragments arranged by size: 3079 988 799 281 246 53 HindIII A'AGCT_T Cuts at: 748 2303 2502 2516 748 Size: 1555 199 14 3678 Fragments arranged by size: 3678 1555 199 14 HpaI GTT'AAC Cuts at: 3036 3036 Size: 5446 KasI G'GCGC_C Cuts at: 2093 2093 Size: 5446 KpnI G_GTAC'C Cuts at: 2771 2771 Size: 5446 MfeI C'AATT_G Cuts at: 2680 3045 2680 Size: 365 5081 MluI A'CGCG_T Cuts at: 5322 5322 Size: 5446 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4560 4744 1305 Size: 3255 184 2007 Fragments arranged by size: 3255 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3624 Fragments arranged by size: 3624 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3404 3763 3922 518 Size: 648 2238 359 159 2042 Fragments arranged by size: 2238 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2045 2862 3539 4687 4932 1606 Size: 439 817 677 1148 245 2120 Fragments arranged by size: 2120 1148 817 677 439 245 NaeI GCC'GGC Cuts at: 2092 2092 Size: 5446 NarI GG'CG_CC Cuts at: 2094 2094 Size: 5446 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3955 Fragments arranged by size: 3955 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5446 NgoMIV G'CCGG_C Cuts at: 2090 2090 Size: 5446 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5446 NotI GC'GGCC_GC Cuts at: 2893 2893 Size: 5446 NspI r_CATG'y Cuts at: 1132 1229 2648 2804 4349 1132 Size: 97 1419 156 1545 2229 Fragments arranged by size: 2229 1545 1419 156 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5446 PciI A'CATG_T Cuts at: 2644 4345 2644 Size: 1701 3745 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5446 PmeI GTTT'AAAC Cuts at: 2750 2750 Size: 5446 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3515 3547 5028 5060 3515 Size: 32 1481 32 3901 Fragments arranged by size: 3901 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5446 PsiI TTA'TAA Cuts at: 3016 3016 Size: 5446 PstI C_TGCA'G Cuts at: 830 2721 2798 830 Size: 1891 77 3478 Fragments arranged by size: 3478 1891 77 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5446 SacII CC_GC'GG Cuts at: 2046 2046 Size: 5446 SalI G'TCGA_C Cuts at: 1936 2788 1936 Size: 852 4594 SbfI CC_TGCA'GG Cuts at: 2798 2798 Size: 5446 ScaI AGT'ACT Cuts at: 3694 5406 3694 Size: 1712 3734 SfcI C'TryA_G Cuts at: 826 1045 2609 2717 2794 3929 4610 4801 826 Size: 219 1564 108 77 1135 681 191 1471 Fragments arranged by size: 1564 1471 1135 681 219 191 108 77 SfoI GGC'GCC Cuts at: 2095 2095 Size: 5446 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5446 SgrAI Cr'CCGG_yG Cuts at: 1700 2090 1700 Size: 390 5056 SmaI CCC'GGG Cuts at: 1872 2773 1872 Size: 901 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2702 2723 3550 4451 4713 820 Size: 197 926 759 21 827 901 262 1553 Fragments arranged by size: 1553 926 901 827 759 262 197 21 SnaBI TAC'GTA Cuts at: 493 3237 493 Size: 2744 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5446 SphI G_CATG'C Cuts at: 1132 2804 1132 Size: 1672 3774 SspI AAT'ATT Cuts at: 5 52 3370 5 Size: 47 3318 2081 Fragments arranged by size: 3318 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5446 StyI C'CwwG_G Cuts at: 513 1065 2004 2885 513 Size: 552 939 881 3074 Fragments arranged by size: 3074 939 881 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3473 3490 3643 3828 4273 325 Size: 1255 1893 17 153 185 445 1498 Fragments arranged by size: 1893 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3692 5404 96 Size: 274 80 33 51 1443 1715 1712 138 Fragments arranged by size: 1715 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5406 5438 5406 Size: 32 5414 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5446 XbaI T'CTAG_A Cuts at: 2782 2782 Size: 5446 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5446 XmaI C'CCGG_G Cuts at: 1870 2771 1870 Size: 901 4545 XmnI GAAnn'nnTTC Cuts at: 2712 3575 2712 Size: 863 4583 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3254 276 Size: 53 83 186 936 1720 2468 Fragments arranged by size: 2468 1720 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI XcmI