################################## # NO CUT ENZYMES ( 184 enzymes) # ################################## AatII Acc65I AccI AccII AciI AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BbvI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsiEI BsiWI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstYI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DraI DraII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI Fnu4HI FseI FspI HaeII HhaI Hin1I HinP1I HincII HindII HindIII HpaI ItaI KasI KpnI KspI MaeII MamI MfeI MflI MluI MluNI MscI MspA1I MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TseI Tth111I TthHB8I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AccIII 285 TCCGGA T^CCGGA AcsI 115 AAATTT [GA]^AATT[CT] ApoI 115 AAATTT [GA]^AATT[CT] AvaII 329 GGACC G^G[AT]CC BanII 247 GAGCTC G[GA]GC[CT]^C BcgI 4 GCAGAGCAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BpmI 164 CTGGAG CTGGAG(16/14) BsaJI 215 CCAGGG C^C[ACGT][ACGT]GG BsaWI 285 TCCGGA [AT]^CCGG[AT] BseAI 285 TCCGGA T^CCGGA BseRI 43 GAGGAG GAGGAG(10/8) BsgI 88 CTGCAC GTGCAG(16/14) BspEI 285 TCCGGA T^CCGGA BstXI 113 CCAAATTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr13I 329 GGACC G^G[ACGT]CC DdeI 179 CTGAG C^T[ACGT]AG DraIII 94 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 362 TGGCCG [CT]^GGCC[GA] EarI 124 GAAGAG CTCTTC(1/4) HapII 286 CCGG C^CGG HgaI 172 GACGC GACGC(5/10) HpaII 286 CCGG C^CGG Ksp632I 124 GAAGAG CTCTTC(1/4) MaeIII 170 GTGAC ^GT[ACGT]AC MroI 285 TCCGGA T^CCGGA MspI 286 CCGG C^CGG NlaIII 390 CATG CATG^ PflMI 320 CCAGTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PstI 356 CTGCAG CTGCA^G SacI 247 GAGCTC GAGCT^C SapI 124 GAAGAGC GCTCTTC(1/4) Sau96I 329 GGACC G^G[ACGT]CC ScaI 29 AGTACT AGT^ACT Tsp45I 170 GTGAC ^GT[GC]AC Tsp509I 116 AATT ^AATT TspRI 359 CAGTG CA[GC]TG[ACGT][ACGT]^ Van91I 320 CCAGTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 350 CCATCTCTGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AfaI 30 GTAC GT^AC 102 GTAC AluI 162 AGCT AG^CT 248 AGCT AlwI 84 GATCC GGATC(4/5) 342 GATCC AspHI 72 GAGCAC G[AT]GC[AT]^C 247 GAGCTC BfaI 40 CTAG C^TAG 314 CTAG BsiHKAI 72 GAGCAC G[AT]GC[AT]^C 247 GAGCTC BsiYI 320 CCAGTTGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 384 CCTATACATGG BslI 320 CCAGTTGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 384 CCTATACATGG BsrI 335 ACTGG ACTGG(1/-1) 320 CCAGT DpnII 84 GATC ^GATC 342 GATC HaeIII 21 GGCC GG^CC 363 GGCC HinfI 48 GATTC G^A[ACGT]TC 310 GATTC HphI 169 GGTGA GGTGA(8/7) 339 GGTGA MaeI 40 CTAG C^TAG 314 CTAG MboI 84 GATC ^GATC 342 GATC MboII 124 GAAGA GAAGA(8/7) 142 GAAGA MseI 151 TTAA T^TAA 274 TTAA MslI 130 CATCCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 253 CATCCAGATG MwoI 81 GCTGATCCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 349 GCCATCTCTGC NdeII 84 GATC ^GATC 342 GATC RsaI 30 GTAC GT^AC 102 GTAC Sau3AI 84 GATC ^GATC 342 GATC SfaNI 211 GATGC GCATC(5/9) 129 GCATC SfcI 206 CTACAG C^T[GA][CT]AG 356 CTGCAG TfiI 48 GATTC G^A[AT]TC 310 GATTC Tru9I 151 TTAA T^TAA 274 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 10 enzymes) # ######################################### BmyI Bsp1286I BssKI BstF5I BstNI EcoRII FokI MnlI MvaI ScrFI