(Linear) (Six-base) MAPSORT of: KIEE6113.seq  Check: 4535  from: 1  to: 402



KIEE6113.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:24  ..

ApoI r'AATT_y

Cuts at:      0     115     402 
   Size:        115     287
 
BanII G_rGCy'C

Cuts at:      0     251     402 
   Size:        251     151
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      27     402 
   Size:         27     375
 
Bme1580I G_kGCm'C

Cuts at:      0     203     402 
   Size:        203     199
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     345     402 
   Size:        345      57
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     185     402 
   Size:        185     217
 
BsaWI w'CCGG_w

Cuts at:      0     285     402 
   Size:        285     117
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      58     402 
   Size:         58     344
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      73     402 
   Size:         73     329
 
BsiHKAI G_wGCw'C

Cuts at:      0      76     251     402 
   Size:         76     175     151

  Fragments arranged by size: 

                 175     151      76
 
Bsp1286I G_dGCh'C

Cuts at:      0      76     203     251     402 
   Size:         76     127      48     151

  Fragments arranged by size: 

                 151     127      76      48
 
BspEI T'CCGG_A

Cuts at:      0     285     402 
   Size:        285     117
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     120     402 
   Size:        120     282
 
BtsI GCAGTG_nn'

Cuts at:      0     365     402 
   Size:        365      37
 
DraIII CAC_nnn'GTG

Cuts at:      0      99     402 
   Size:         99     303
 
EaeI y'GGCC_r

Cuts at:      0     362     402 
   Size:        362      40
 
EarI CTCTTCn'nnn_

Cuts at:      0     119     402 
   Size:        119     283
 
EcoICRI GAG'CTC

Cuts at:      0     249     402 
   Size:        249     153
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     185     402 
   Size:        185     217
 
MslI CAynn'nnrTG

Cuts at:      0     134     257     402 
   Size:        134     123     145

  Fragments arranged by size: 

                 145     134     123
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     326     402 
   Size:        326      76
 
PstI C_TGCA'G

Cuts at:      0     360     402 
   Size:        360      42
 
SacI G_AGCT'C

Cuts at:      0     251     402 
   Size:        251     151
 
SapI GCTCTTCn'nnn_

Cuts at:      0     119     402 
   Size:        119     283
 
ScaI AGT'ACT

Cuts at:      0      31     402 
   Size:         31     371
 
SfcI C'TryA_G

Cuts at:      0     206     356     402 
   Size:        206     150      46

  Fragments arranged by size: 

                 206     150      46
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     327     402 
   Size:        327      75
 
TatI w'GTAC_w

Cuts at:      0      29     402 
   Size:         29     373
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     357     402 
   Size:        357      45
 

 Enzymes that do cut:

     ApoI    BanII     BcgI Bme1580I     BmrI     BpmI    BsaWI    BseRI
     BsgI  BsiHKAI Bsp1286I    BspEI    BstXI     BtsI   DraIII     EaeI
     EarI  EcoICRI  Eco57MI     MslI    PflMI     PstI     SacI     SapI
     ScaI     SfcI    TaqII     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI     BbeI     BbsI    BbvCI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI    BmgBI     BmtI     BplI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaXI    BseYI    BsiEI    BsiWI     BsmI
    BsmBI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstYI  BstZ17I   Bsu36I     BtgI
    BtgZI     ClaI    CspCI     DraI     DrdI     EagI     EciI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    PvuII    RsrII    SacII     SalI    SanDI
     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI
     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI