Restriction map for
pFN21AE4526
(Circular) (Six-base) MAPSORT of: pFN21AE4526.seq Check: 365 from: 1 to: 6427
pFN21AE4526 6427 bp
With 182 enzymes: SgfI *
August 20, 2012 11:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2143 2143
Size: 6427
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4237 278
Size: 53 83 186 936 2701 2468
Fragments arranged by size:
2701 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3770 1937
Size: 1833 4594
Acc65I G'GTAC_C
Cuts at: 2931 3748 2931
Size: 817 5610
AclI AA'CG_TT
Cuts at: 1563 4554 4927 1563
Size: 2991 373 3063
Fragments arranged by size:
3063 2991 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4490 5874 6142 1632
Size: 2858 1384 268 1917
Fragments arranged by size:
2858 1917 1384 268
AfeI AGC'GCT
Cuts at: 2207 3485 2207
Size: 1278 5149
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6230
AflIII A'CryG_T
Cuts at: 1203 5326 6303 1203
Size: 4123 977 1327
Fragments arranged by size:
4123 1327 977
AgeI A'CCGG_T
Cuts at: 5258 5258
Size: 6427
AhdI GACnn_n'nnGTC
Cuts at: 2584 5156 2584
Size: 2572 3855
AleI CACnn'nnGTG
Cuts at: 1166 3155 1166
Size: 1989 4438
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3724 1184
Size: 2540 3887
AlwNI CAG_nnn'CTG
Cuts at: 3444 3462 3514 4170 5742 3444
Size: 18 52 656 1572 4129
Fragments arranged by size:
4129 1572 656 52 18
ApaI G_GGCC'C
Cuts at: 2041 2431 2041
Size: 390 6037
ApaLI G'TGCA_C
Cuts at: 2072 3608 4484 5640 2072
Size: 1536 876 1156 2859
Fragments arranged by size:
2859 1536 1156 876
ApoI r'AATT_y
Cuts at: 1493 3736 3962 4114 6223 1493
Size: 2243 226 152 2109 1697
Fragments arranged by size:
2243 2109 1697 226 152
AscI GG'CGCG_CC
Cuts at: 2241 2241
Size: 6427
AseI AT'TA_AT
Cuts at: 160 1032 4981 160
Size: 872 3949 1606
Fragments arranged by size:
3949 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6427
AvaI C'yCGr_G
Cuts at: 1870 1943 2016 2093 2173 2223 2424 2694 2735
Size: 73 73 77 80 50 201 270 41
Cuts at: 2735 2768 2888 2957 3752 1870
Size: 33 120 69 795 4545
Fragments arranged by size:
4545 795 270 201 120 80 77 73
73 69 50 41 33
BamHI G'GATC_C
Cuts at: 3757 4124 3757
Size: 367 6060
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2051 2293 2352 2374 2548
Size: 325 217 627 264 242 59 22 174
Cuts at: 2548 2849 2931 3748 5203 618
Size: 301 82 817 1455 1842
Fragments arranged by size:
1842 1455 817 627 325 301 264 242
217 174 82 59 22
BanII G_rGCy'C
Cuts at: 1391 2041 2105 2180 2279 2327 2431 2465 3169
Size: 650 64 75 99 48 104 34 704
Cuts at: 3169 3521 3746 1391
Size: 352 225 4072
Fragments arranged by size:
4072 704 650 352 225 104 99 75
64 48 34
BbeI G_GCGC'C
Cuts at: 2055 2297 2552 2055
Size: 242 255 5930
Fragments arranged by size:
5930 255 242
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5456
BbvCI CC'TCA_GC
Cuts at: 2202 3065 2202
Size: 863 5564
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4618 4652 1903
Size: 34 2681 34 3678
Fragments arranged by size:
3678 2681 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4319 5529 4319
Size: 1210 5217
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6427
BfrBI ATG'CAT
Cuts at: 3491 3491
Size: 6427
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3233 5038 136
Size: 107 122 71 631 605 1561 1805 1525
Fragments arranged by size:
1805 1561 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6422 6422
Size: 6427
BlpI GC'TnA_GC
Cuts at: 3844 3844
Size: 6427
Bme1580I G_kGCm'C
Cuts at: 1790 2041 2076 2379 2431 2852 3296 3612 4488
Size: 251 35 303 52 421 444 316 876
Cuts at: 4488 5644 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 876 444 421 316 303 251
52 35
BmgBI CAC'GTC
Cuts at: 1318 3580 1318
Size: 2262 4165
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4158 5116 442
Size: 416 53 83 399 24 2741 958 1753
Fragments arranged by size:
2741 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6427
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2236 2236
Size: 6427
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3005 3470 5087 1239
Size: 1766 465 1617 2579
Fragments arranged by size:
2579 1766 1617 465
Bpu10I CC'TnA_GC
Cuts at: 2202 3065 3515 2202
Size: 863 450 5114
Fragments arranged by size:
5114 863 450
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3464 3587 4552 5417 5715 3464
Size: 123 965 865 298 4176
Fragments arranged by size:
4176 965 865 298 123
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2482 2907 3113 5090 882
Size: 617 983 425 206 1977 2219
Fragments arranged by size:
2219 1977 983 617 425 206
BsaAI yAC'GTr
Cuts at: 493 1204 2188 2782 3613 4218 493
Size: 711 984 594 831 605 2702
Fragments arranged by size:
2702 984 831 711 605 594
BsaBI GATnn'nnATC
Cuts at: 6421 6421
Size: 6427
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2052 2284 2294 2549
Size: 53 83 186 936 519 232 10 255
Cuts at: 2549 4234 4616 5251 275
Size: 1685 382 635 1451
Fragments arranged by size:
1685 1451 936 635 519 382 255 232
186 83 53 10
BsaWI w'CCGG_w
Cuts at: 4860 5258 5532 5679 6090 4860
Size: 398 274 147 411 5197
Fragments arranged by size:
5197 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2447 2477 3161 3188 3191
Size: 30 165 30 1044 30 684 27 3
Cuts at: 3191 3218 1178
Size: 27 4387
Fragments arranged by size:
4387 1044 684 165 30 30 30 27
27 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2461 2723 3212 1186
Size: 1275 262 489 4401
Fragments arranged by size:
4401 1275 489 262
BseYI C'CCAG_C
Cuts at: 2032 2253 2445 3273 5630 6126 2032
Size: 221 192 828 2357 496 2333
Fragments arranged by size:
2357 2333 828 496 221 192
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3179 3602 1163
Size: 573 1443 423 3988
Fragments arranged by size:
3988 1443 573 423
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2196 2658 3103 3877 4638 5666
Size: 275 186 195 462 445 774 761 1028
Cuts at: 5666 1540
Size: 2301
Fragments arranged by size:
2301 1028 774 761 462 445 275 195
186
BsiHKAI G_wGCw'C
Cuts at: 2076 3612 4488 4573 5644 2076
Size: 1536 876 85 1071 2859
Fragments arranged by size:
2859 1536 1071 876 85
BsiWI C'GTAC_G
Cuts at: 2964 2964
Size: 6427
BsmI GAATG_Cn'
Cuts at: 3938 4031 3938
Size: 93 6334
BsmBI CGTCTCn'nnnn_
Cuts at: 2446 2446
Size: 6427
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2041 2076 2105 2180 2279 2327 2379
Size: 399 251 35 29 75 99 48 52
Cuts at: 2379 2431 2465 2852 3169 3296 3521 3612 3746
Size: 52 34 387 317 127 225 91 134
Cuts at: 3746 4488 4573 5644 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 387 317 251 225
134 127 99 91 85 75 52 52
48 35 34 29
BspEI T'CCGG_A
Cuts at: 6090 6090
Size: 6427
BspHI T'CATG_A
Cuts at: 4316 6046 4316
Size: 1730 4697
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2143 3006 3782 844
Size: 1299 863 776 3489
Fragments arranged by size:
3489 1299 863 776
BsrBI CCG'CTC
Cuts at: 1394 1823 3040 4314 1394
Size: 429 1217 1274 3507
Fragments arranged by size:
3507 1274 1217 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4922 5096 65
Size: 1183 3674 174 1396
Fragments arranged by size:
3674 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2025 2191 2237 2619 3001 3084 3315
Size: 110 215 166 46 382 382 83 231
Cuts at: 3315 5071 5258 1700
Size: 1756 187 2869
Fragments arranged by size:
2869 1756 382 382 231 215 187 166
110 83 46
BsrGI T'GTAC_A
Cuts at: 96 2157 2838 96
Size: 2061 681 3685
Fragments arranged by size:
3685 2061 681
BssHII G'CGCG_C
Cuts at: 1925 2241 2699 2977 2979 3048 1925
Size: 316 458 278 2 69 5304
Fragments arranged by size:
5304 458 316 278 69 2
BssSI C'ACGA_G
Cuts at: 4487 5499 4487
Size: 1012 5415
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6427
BstBI TT'CG_AA
Cuts at: 5231 5231
Size: 6427
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6427
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 3658 2011
Size: 1647 4780
BstYI r'GATC_y
Cuts at: 1972 3757 4124 4509 4526 5978 6064 6076 6422
Size: 1785 367 385 17 1452 86 12 346
Cuts at: 6422 1972
Size: 1977
Fragments arranged by size:
1977 1785 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2748 3231 2748
Size: 483 5944
BtgI C'CryG_G
Cuts at: 513 1065 2004 2269 2625 2658 2905 3103 3320
Size: 552 939 265 356 33 247 198 217
Cuts at: 3320 513
Size: 3620
Fragments arranged by size:
3620 939 552 356 265 247 217 198
33
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5688
Fragments arranged by size:
5688 683 56
BtsI GCAGTG_nn'
Cuts at: 3943 4737 4757 3943
Size: 794 20 5613
Fragments arranged by size:
5613 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6427
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6392
DraI TTT'AAA
Cuts at: 3731 3731
Size: 6427
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5434 809
Size: 507 4118 1802
Fragments arranged by size:
4118 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2193 2235 2341
Size: 54 1425 50 275 18 363 42 106
Cuts at: 2341 2368 2397 2655 3100 3210 3317 3420 3874
Size: 27 29 258 445 110 107 103 454
Cuts at: 3874 4763 6392 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 454 445 363 275 258
110 107 106 103 54 50 43 42
29 27 18
EagI C'GGCC_G
Cuts at: 1537 1812 2193 2655 3100 3874 1537
Size: 275 381 462 445 774 4090
Fragments arranged by size:
4090 774 462 445 381 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2721 4357 1353
Size: 1368 1636 3423
Fragments arranged by size:
3423 1636 1368
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2455 2925 5014 5388 5534 387
Size: 1064 138 866 470 2089 374 146 1280
Fragments arranged by size:
2089 1280 1064 866 470 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3005 3470 4490 5087 5874 6142 1239
Size: 393 1373 465 1020 597 787 268 1524
Fragments arranged by size:
1524 1373 1020 787 597 465 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2037 2038 2775 3511 2037
Size: 1 737 736 4953
Fragments arranged by size:
4953 737 736 1
EcoRI G'AATT_C
Cuts at: 3736 4114 3736
Size: 378 6049
FspI TGC'GCA
Cuts at: 3008 4933 3008
Size: 1925 4502
HaeII r_GCGC'y
Cuts at: 1678 2055 2209 2297 2552 3487 5574 1678
Size: 377 154 88 255 935 2087 2531
Fragments arranged by size:
2531 2087 935 377 255 154 88
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2570 2602 5068 5100 5142
Size: 32 135 32 998 32 2466 32 42
Cuts at: 5142 5174 1373
Size: 32 2626
Fragments arranged by size:
2626 2466 998 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3771 4017 669
Size: 988 281 1833 246 3079
Fragments arranged by size:
3079 1833 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6427
HpaI GTT'AAC
Cuts at: 4017 4017
Size: 6427
KasI G'GCGC_C
Cuts at: 2051 2293 2548 2051
Size: 242 255 5930
Fragments arranged by size:
5930 255 242
KpnI G_GTAC'C
Cuts at: 2935 3752 2935
Size: 817 5610
MfeI C'AATT_G
Cuts at: 4026 4026
Size: 6427
MluI A'CGCG_T
Cuts at: 6303 6303
Size: 6427
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3039 5541 5725 1305
Size: 1734 2502 184 2007
Fragments arranged by size:
2502 2007 1734 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2370 3212 10
Size: 54 1425 343 538 842 3225
Fragments arranged by size:
3225 1425 842 538 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2417 3155 4385 4744 4903 518
Size: 648 1251 738 1230 359 159 2042
Fragments arranged by size:
2042 1251 1230 738 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2032 2118 2128 2260 2627 3105 3345 3843
Size: 426 86 10 132 367 478 240 498
Cuts at: 3843 4520 5668 5913 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 498 478 426 367 245
240 132 86 10
NaeI GCC'GGC
Cuts at: 2027 2193 2239 2027
Size: 166 46 6215
Fragments arranged by size:
6215 166 46
NarI GG'CG_CC
Cuts at: 2052 2294 2549 2052
Size: 242 255 5930
Fragments arranged by size:
5930 255 242
NcoI C'CATG_G
Cuts at: 513 1065 2004 2905 513
Size: 552 939 901 4035
Fragments arranged by size:
4035 939 901 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6427
NgoMIV G'CCGG_C
Cuts at: 2025 2191 2237 2025
Size: 166 46 6215
Fragments arranged by size:
6215 166 46
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6427
NotI GC'GGCC_GC
Cuts at: 3874 3874
Size: 6427
NsiI A_TGCA'T
Cuts at: 3493 3493
Size: 6427
NspI r_CATG'y
Cuts at: 1132 1229 3495 3785 5330 1132
Size: 97 2266 290 1545 2229
Fragments arranged by size:
2266 2229 1545 290 97
PasI CC'CwG_GG
Cuts at: 1348 3508 1348
Size: 2160 4267
PciI A'CATG_T
Cuts at: 5326 5326
Size: 6427
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3367 1108
Size: 2259 4168
PmeI GTTT'AAAC
Cuts at: 3731 3731
Size: 6427
PmlI CAC'GTG
Cuts at: 3613 3613
Size: 6427
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4496 4528 6009 6041 4496
Size: 32 1481 32 4882
Fragments arranged by size:
4882 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2883 1613
Size: 1270 5157
PsiI TTA'TAA
Cuts at: 3997 3997
Size: 6427
PspOMI G'GGCC_C
Cuts at: 2037 2427 2037
Size: 390 6037
PstI C_TGCA'G
Cuts at: 830 3779 830
Size: 2949 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6427
PvuII CAG'CTG
Cuts at: 2032 2128 3345 2032
Size: 96 1217 5114
Fragments arranged by size:
5114 1217 96
SacII CC_GC'GG
Cuts at: 2628 3106 2628
Size: 478 5949
SalI G'TCGA_C
Cuts at: 1936 3769 1936
Size: 1833 4594
SapI GCTCTTCn'nnn_
Cuts at: 2721 2721
Size: 6427
SbfI CC_TGCA'GG
Cuts at: 3779 3779
Size: 6427
ScaI AGT'ACT
Cuts at: 4675 6387 4675
Size: 1712 4715
SfcI C'TryA_G
Cuts at: 826 1045 3775 4910 5591 5782 826
Size: 219 2730 1135 681 191 1471
Fragments arranged by size:
2730 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2053 2295 2550 2053
Size: 242 255 5930
Fragments arranged by size:
5930 255 242
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6427
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6427
SmaI CCC'GGG
Cuts at: 1872 2095 2225 2426 2696 3754 1872
Size: 223 130 201 270 1058 4545
Fragments arranged by size:
4545 1058 270 223 201 130
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3479 3602 4531 5432 5694 820
Size: 197 926 1536 123 929 901 262 1553
Fragments arranged by size:
1553 1536 929 926 901 262 197 123
SnaBI TAC'GTA
Cuts at: 493 4218 493
Size: 3725 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6427
SphI G_CATG'C
Cuts at: 1132 3495 3785 1132
Size: 2363 290 3774
Fragments arranged by size:
3774 2363 290
SrfI GCCC'GGGC
Cuts at: 2095 2426 2095
Size: 331 6096
SspI AAT'ATT
Cuts at: 5 52 4351 5
Size: 47 4299 2081
Fragments arranged by size:
4299 2081 47
StuI AGG'CCT
Cuts at: 1517 2870 1517
Size: 1353 5074
StyI C'CwwG_G
Cuts at: 513 1065 2004 2905 3866 513
Size: 552 939 901 961 3074
Fragments arranged by size:
3074 961 939 901 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2370 2464 4454 4471 4624 4809 5254
Size: 1255 790 94 1990 17 153 185 445
Cuts at: 5254 325
Size: 1498
Fragments arranged by size:
1990 1498 1255 790 445 185 153 94
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2157 2613 2838
Size: 274 80 33 51 1443 180 456 225
Cuts at: 2838 3174 4673 6385 96
Size: 336 1499 1712 138
Fragments arranged by size:
1712 1499 1443 456 336 274 225 180
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2447 2479 3161 3188 3193 3220 6387 6419 2447
Size: 32 682 27 5 27 3167 32 2455
Fragments arranged by size:
3167 2455 682 32 32 27 27 5
Tth111I GACn'n_nGTC
Cuts at: 1316 3119 1316
Size: 1803 4624
XbaI T'CTAG_A
Cuts at: 3763 3763
Size: 6427
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2925 3327 3445 2925
Size: 402 118 5907
Fragments arranged by size:
5907 402 118
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6427
XmaI C'CCGG_G
Cuts at: 1870 2093 2223 2424 2694 3752 1870
Size: 223 130 201 270 1058 4545
Fragments arranged by size:
4545 1058 270 223 201 130
XmnI GAAnn'nnTTC
Cuts at: 4556 4556
Size: 6427
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4235 276
Size: 53 83 186 936 2701 2468
Fragments arranged by size:
2701 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI
PvuII SacII SalI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AvrII BaeI BstZ17I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI NruI PacI PfoI PpuMI PspXI
PsrI RsrII SacI SanDI SexAI SfiI SwaI