Restriction map for
pFN21AE4473
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# NO CUT ENZYMES ( 111 enzymes) #
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Acc65I AccIII AclI AcsI AflII
AflIII AgeI AhdI AocI ApaI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AvrII BamHI BbeI
BbrPI BbsI BbvCI BciVI BclI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BpuAI BsaBI BsaI BseAI
BsiWI BsmBI BsmFI BsmI Bsp1407I
BspEI BspHI BspLU11I BsrDI BsrGI
BssHII BssSI Bst1107I BstEII BstPI
BstZ17I Bsu36I CelII CpoI DraI
EagI Eam1105I EclXI Eco52I Eco81I
EcoO65I EcoRI EcoRV FbaI FseI
HindIII HpaI KasI KpnI KspI
MaeI MamI MfeI MluI MroI
MunI NarI NdeI NheI NotI
NruI PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacII
SapI ScaI SexAI SfiI SfoI
SgrAI SmlI SpeI SspBI SspI
SwaI TfiI Tsp509I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccI 276 GTCGAC GT^[AC][GT]AC
AfaI 381 GTAC GT^AC
Alw44I 1126 GTGCAC G^TGCAC
ApaLI 1126 GTGCAC G^TGCAC
AspI 370 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 695 CCCGGG C^[CT]CG[GA]G
AviII 1190 TGCGCA TGC^GCA
BalI 1104 TGGCCA TGG^CCA
BcgI 1320 GCAGCGCTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 1092 GCCTCACGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 382 TACGTA [CT]AC^GT[GA]
BsaWI 13 ACCGGA [AT]^CCGG[AT]
BsiEI 789 CGACCG CG[GA][CT]^CG
BsmAI 157 GAGAC GTCTC(1/5)
BsoBI 695 CCCGGG C^[CT]CG[GA]G
BspDI 256 ATCGAT AT^CGAT
BstAPI 464 GCAGTGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 505 TTCGAA TT^CGAA
BstYI 329 AGATCC [GA]^GATC[CT]
ClaI 256 ATCGAT AT^CGAT
DraIII 1129 CACGACGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 1263 CCTGGATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 1431 ATGCAT ATGCA^T
FspI 1190 TGCGCA TGC^GCA
MflI 329 AGATCC [GA]^GATC[CT]
MluNI 1104 TGGCCA TGG^CCA
MscI 1104 TGGCCA TGG^CCA
NaeI 124 GCCGGC GCC^GGC
NcoI 1058 CCATGG C^CATGG
NgoMIV 124 GCCGGC G^CCGGC
NsiI 1431 ATGCAT ATGCA^T
NspV 505 TTCGAA TT^CGAA
PflFI 370 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
RsaI 381 GTAC GT^AC
SalI 276 GTCGAC G^TCGAC
SbfI 141 CCTGCAGG CCTGCA^GG
SfuI 505 TTCGAA TT^CGAA
SmaI 695 CCCGGG CCC^GGG
SnaBI 382 TACGTA TAC^GTA
Sse8387I 141 CCTGCAGG CCTGCA^GG
Tth111I 370 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 964 CCAGGTGTCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 329 AGATCC [GA]^GATC[CT]
XmaCI 695 CCCGGG C^CCGGG
XmaI 695 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 23 enzymes) #
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AlwI 330 GATCC GGATC(4/5)
798 GATCC
AlwNI 82 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG
465 CAGTGTCTG
Aor51HI 164 AGCGCT AGC^GCT
1322 AGCGCT
BsrBI 94 GAGCGG CCGCTC(-3/-3)
1041 GAGCGG
BsrFI 77 ACCGGC [GA]^CCGG[CT]
124 GCCGGC
BstXI 314 CCACCTGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
964 CCAGGTGTCTGG
Cfr10I 77 ACCGGC [GA]^CCGG[CT]
124 GCCGGC
DrdI 178 GACACCGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1054 GACGCCATGGTC
EaeI 8 CGGCCA [CT]^GGCC[GA]
1104 TGGCCA
Eco47III 164 AGCGCT AGC^GCT
1322 AGCGCT
EcoT14I 338 CCAAGG C^C[AT][AT]GG
1058 CCATGG
HinfI 159 GACTC G^A[ACGT]TC
202 GAGTC
MseI 557 TTAA T^TAA
1304 TTAA
MslI 315 CACCTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1119 CATTGACGTG
PflMI 1107 CCACTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1257 CCACCTCCTGG
PleI 159 GACTC GAGTC(4/5)
202 GAGTC
PpuMI 185 GGGTCCT [GA]G^G[AT]CC[CT]
1313 AGGTCCT
SacI 779 GAGCTC GAGCT^C
933 GAGCTC
SphI 704 GCATGC GCATG^C
1429 GCATGC
StuI 479 AGGCCT AGG^CCT
1451 AGGCCT
StyI 338 CCAAGG C^C[AT][AT]GG
1058 CCATGG
Tru9I 557 TTAA T^TAA
1304 TTAA
Van91I 1107 CCACTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1257 CCACCTCCTGG
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# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AatII AccII AciI AcyI AluI
AspHI AvaII BanI BanII BbvI
BcnI BmyI BpmI BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EarI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MnlI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PstI Sau3AI
Sau96I ScrFI SfaNI SfcI TaqI
TseI Tsp45I TspRI TthHB8I