(Circular) (Six-base) MAPSORT of: pFN21AE4473.seq Check: 6024 from: 1 to: 6184 pFN21AE4473 6184 bp With 182 enzymes: SgfI * March 28, 2012 13:56 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2738 2879 3219 2738 Size: 141 340 5703 Fragments arranged by size: 5703 340 141 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2382 2417 3369 3994 Size: 53 83 186 936 846 35 952 625 Cuts at: 3994 278 Size: 2468 Fragments arranged by size: 2468 952 936 846 625 186 83 53 35 AccI GT'mk_AC Cuts at: 1937 2282 3527 1937 Size: 345 1245 4594 Fragments arranged by size: 4594 1245 345 Acc65I G'GTAC_C Cuts at: 3505 3505 Size: 6184 AclI AA'CG_TT Cuts at: 1563 4311 4684 1563 Size: 2748 373 3063 Fragments arranged by size: 3063 2748 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2072 4247 5631 5899 1632 Size: 440 2175 1384 268 1917 Fragments arranged by size: 2175 1917 1384 440 268 AfeI AGC'GCT Cuts at: 2171 3329 2171 Size: 1158 5026 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5987 AflIII A'CryG_T Cuts at: 1203 5083 6060 1203 Size: 3880 977 1327 Fragments arranged by size: 3880 1327 977 AgeI A'CCGG_T Cuts at: 5015 5015 Size: 6184 AhdI GACnn_n'nnGTC Cuts at: 4913 4913 Size: 6184 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6184 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3146 3410 1184 Size: 1962 264 3958 Fragments arranged by size: 3958 1962 264 AlwNI CAG_nnn'CTG Cuts at: 2092 2475 3927 5499 2092 Size: 383 1452 1572 2777 Fragments arranged by size: 2777 1572 1452 383 ApaLI G'TGCA_C Cuts at: 3131 4241 5397 3131 Size: 1110 1156 3918 Fragments arranged by size: 3918 1156 1110 ApoI r'AATT_y Cuts at: 1493 3493 3719 3871 5980 1493 Size: 2000 226 152 2109 1697 Fragments arranged by size: 2109 2000 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4738 160 Size: 872 3706 1606 Fragments arranged by size: 3706 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6184 AvaI C'yCGr_G Cuts at: 1870 1943 2700 3509 1870 Size: 73 757 809 4545 Fragments arranged by size: 4545 809 757 73 BamHI G'GATC_C Cuts at: 3514 3881 3514 Size: 367 5817 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2056 2957 3207 3505 4960 Size: 325 217 627 269 901 250 298 1455 Cuts at: 4960 618 Size: 1842 Fragments arranged by size: 1842 1455 901 627 325 298 269 250 217 BanII G_rGCy'C Cuts at: 1391 2788 2942 3180 3503 1391 Size: 1397 154 238 323 4072 Fragments arranged by size: 4072 1397 323 238 154 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5213 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3314 3348 4375 4409 1903 Size: 34 1377 34 1027 34 3678 Fragments arranged by size: 3678 1377 1027 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4076 5286 4076 Size: 1210 4974 BclI T'GATC_A Cuts at: 1548 1548 Size: 6184 BfrBI ATG'CAT Cuts at: 3438 3438 Size: 6184 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 3103 4795 Size: 107 122 71 631 605 337 1094 1692 Cuts at: 4795 136 Size: 1525 Fragments arranged by size: 1692 1525 1094 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 6179 6179 Size: 6184 BlpI GC'TnA_GC Cuts at: 3601 3601 Size: 6184 Bme1580I G_kGCm'C Cuts at: 1790 2298 3135 3411 4245 5401 1790 Size: 508 837 276 834 1156 2573 Fragments arranged by size: 2573 1156 837 834 508 276 BmgBI CAC'GTC Cuts at: 1318 3130 3139 1318 Size: 1812 9 4363 Fragments arranged by size: 4363 1812 9 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2230 2283 3915 Size: 416 53 83 399 24 813 53 1632 Cuts at: 3915 4873 442 Size: 958 1753 Fragments arranged by size: 1753 1632 958 813 416 399 83 53 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6184 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2593 2600 3038 4844 1239 Size: 1354 7 438 1806 2579 Fragments arranged by size: 2579 1806 1354 438 7 Bpu10I CC'TnA_GC Cuts at: 3044 3044 Size: 6184 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4309 5174 5472 4309 Size: 865 298 5021 Fragments arranged by size: 5021 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4847 882 Size: 617 3348 2219 Fragments arranged by size: 3348 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2389 3975 493 Size: 711 1185 1586 2702 Fragments arranged by size: 2702 1586 1185 711 BsaBI GATnn'nnATC Cuts at: 6178 6178 Size: 6184 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2379 2414 2451 3060 Size: 53 83 186 936 846 35 37 609 Cuts at: 3060 3366 3991 4373 5008 275 Size: 306 625 382 635 1451 Fragments arranged by size: 1451 936 846 635 625 609 382 306 186 83 53 37 35 BsaWI w'CCGG_w Cuts at: 2018 4617 5015 5289 5436 5847 2018 Size: 2599 398 274 147 411 2355 Fragments arranged by size: 2599 2355 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2681 2711 2929 2959 1178 Size: 30 165 30 1278 30 218 30 4403 Fragments arranged by size: 4403 1278 218 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2174 2426 3035 3374 1186 Size: 988 252 609 339 3996 Fragments arranged by size: 3996 988 609 339 252 BseYI C'CCAG_C Cuts at: 2035 5387 5883 2035 Size: 3352 496 2336 Fragments arranged by size: 3352 2336 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2144 2161 3186 1163 Size: 573 408 17 1025 4161 Fragments arranged by size: 4161 1025 573 408 17 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2797 3634 4395 5423 1540 Size: 275 186 796 837 761 1028 2301 Fragments arranged by size: 2301 1028 837 796 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2352 2688 2788 2942 3135 3231 4245 4330 5401 Size: 336 100 154 193 96 1014 85 1071 Cuts at: 5401 2352 Size: 3135 Fragments arranged by size: 3135 1071 1014 336 193 154 100 96 85 BsmI GAATG_Cn' Cuts at: 3695 3788 3695 Size: 93 6091 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2298 2352 2688 2788 2942 3135 3180 Size: 399 508 54 336 100 154 193 45 Cuts at: 3180 3231 3411 3503 4245 4330 5401 1391 Size: 51 180 92 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 508 399 336 193 180 154 100 92 85 54 51 45 BspEI T'CCGG_A Cuts at: 5847 5847 Size: 6184 BspHI T'CATG_A Cuts at: 4073 5803 4073 Size: 1730 4454 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2738 2879 3219 3410 3539 844 Size: 1894 141 340 191 129 3489 Fragments arranged by size: 3489 1894 340 191 141 129 BsrBI CCG'CTC Cuts at: 1394 1823 2101 3048 4071 1394 Size: 429 278 947 1023 3507 Fragments arranged by size: 3507 1023 947 429 278 BsrDI GCAATG_nn' Cuts at: 65 1248 4679 4853 65 Size: 1183 3431 174 1396 Fragments arranged by size: 3431 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2082 2129 4828 5015 1700 Size: 110 272 47 2699 187 2869 Fragments arranged by size: 2869 2699 272 187 110 47 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6184 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6184 BssSI C'ACGA_G Cuts at: 4244 5256 4244 Size: 1012 5172 BstAPI GCAn_nnn'nTGC Cuts at: 794 2475 794 Size: 1681 4503 BstBI TT'CG_AA Cuts at: 2511 4988 2511 Size: 2477 3707 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6184 BstXI CCAn_nnnn'nTGG Cuts at: 2326 2976 2326 Size: 650 5534 BstYI r'GATC_y Cuts at: 1972 2334 3514 3881 4266 4283 5735 5821 5833 Size: 362 1180 367 385 17 1452 86 12 Cuts at: 5833 6179 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1180 385 367 362 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2370 3063 3234 513 Size: 552 939 366 693 171 3463 Fragments arranged by size: 3463 939 693 552 366 171 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5445 Fragments arranged by size: 5445 683 56 BtsI GCAGTG_nn' Cuts at: 2476 2749 2898 2934 3198 3700 4494 4514 2476 Size: 273 149 36 264 502 794 20 4146 Fragments arranged by size: 4146 794 502 273 264 149 36 20 ClaI AT'CG_AT Cuts at: 1554 2262 1554 Size: 708 5476 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6149 DraI TTT'AAA Cuts at: 3488 3488 Size: 6184 DraIII CAC_nnn'GTG Cuts at: 3139 3139 Size: 6184 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2189 3065 5191 809 Size: 507 873 876 2126 1802 Fragments arranged by size: 2126 1802 876 873 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2013 3109 3631 Size: 54 1425 50 275 18 183 1096 522 Cuts at: 3631 4520 6149 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1096 889 522 275 183 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3631 1537 Size: 275 1819 4090 Fragments arranged by size: 4090 1819 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2201 2684 3417 4114 1353 Size: 848 483 733 697 3423 Fragments arranged by size: 3423 848 733 697 483 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2189 3299 4771 5145 5291 387 Size: 1064 138 600 1110 1472 374 146 1280 Fragments arranged by size: 1472 1280 1110 1064 600 374 146 138 EcoICRI GAG'CTC Cuts at: 2786 2940 2786 Size: 154 6030 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2072 2593 2600 3038 4247 4844 5631 Size: 393 440 521 7 438 1209 597 787 Cuts at: 5631 5899 1239 Size: 268 1524 Fragments arranged by size: 1524 1209 787 597 521 440 438 393 268 7 EcoNI CCTnn'n_nnAGG Cuts at: 3272 3272 Size: 6184 EcoO109I rG'GnC_Cy Cuts at: 2191 3277 3319 2191 Size: 1086 42 5056 Fragments arranged by size: 5056 1086 42 EcoRI G'AATT_C Cuts at: 3493 3871 3493 Size: 378 5806 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2368 2368 Size: 6184 FspI TGC'GCA Cuts at: 3197 4690 3197 Size: 1493 4691 HaeII r_GCGC'y Cuts at: 1678 2069 2092 2173 3331 5331 1678 Size: 391 23 81 1158 2000 2531 Fragments arranged by size: 2531 2000 1158 391 81 23 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4825 4857 4899 4931 1373 Size: 32 135 32 3253 32 42 32 2626 Fragments arranged by size: 3253 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2269 2283 2455 2518 3528 3774 Size: 988 281 331 14 172 63 1010 246 Cuts at: 3774 669 Size: 3079 Fragments arranged by size: 3079 1010 988 331 281 246 172 63 14 HindIII A'AGCT_T Cuts at: 748 748 Size: 6184 HpaI GTT'AAC Cuts at: 3774 3774 Size: 6184 KpnI G_GTAC'C Cuts at: 3509 3509 Size: 6184 MfeI C'AATT_G Cuts at: 3783 3783 Size: 6184 MluI A'CGCG_T Cuts at: 6060 6060 Size: 6184 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2649 2886 5298 5482 1305 Size: 1344 237 2412 184 2007 Fragments arranged by size: 2412 2007 1344 237 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3111 10 Size: 54 1425 343 1279 3083 Fragments arranged by size: 3083 1425 1279 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2324 3128 4142 4501 4660 518 Size: 648 1158 804 1014 359 159 2042 Fragments arranged by size: 2042 1158 1014 804 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2062 2466 3384 3600 4277 5425 5670 1606 Size: 456 404 918 216 677 1148 245 2120 Fragments arranged by size: 2120 1148 918 677 456 404 245 216 NaeI GCC'GGC Cuts at: 2131 2131 Size: 6184 NcoI C'CATG_G Cuts at: 513 1065 2004 3063 513 Size: 552 939 1059 3634 Fragments arranged by size: 3634 1059 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6184 NgoMIV G'CCGG_C Cuts at: 2129 2129 Size: 6184 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6184 NotI GC'GGCC_GC Cuts at: 3631 3631 Size: 6184 NsiI A_TGCA'T Cuts at: 3440 3440 Size: 6184 NspI r_CATG'y Cuts at: 1132 1229 2713 2992 3438 3542 5087 1132 Size: 97 1484 279 446 104 1545 2229 Fragments arranged by size: 2229 1545 1484 446 279 104 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6184 PciI A'CATG_T Cuts at: 5083 5083 Size: 6184 PflMI CCAn_nnn'nTGG Cuts at: 1108 3118 3268 1108 Size: 2010 150 4024 Fragments arranged by size: 4024 2010 150 PfoI T'CCnGG_A Cuts at: 3267 3267 Size: 6184 PmeI GTTT'AAAC Cuts at: 3488 3488 Size: 6184 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4253 4285 5766 5798 4253 Size: 32 1481 32 4639 Fragments arranged by size: 4639 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2191 3319 2191 Size: 1128 5056 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6184 PsiI TTA'TAA Cuts at: 3754 3754 Size: 6184 PstI C_TGCA'G Cuts at: 830 2151 2480 2905 3216 3327 3536 830 Size: 1321 329 425 311 111 209 3478 Fragments arranged by size: 3478 1321 425 329 311 209 111 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6184 SacI G_AGCT'C Cuts at: 2788 2942 2788 Size: 154 6030 SalI G'TCGA_C Cuts at: 1936 2281 3526 1936 Size: 345 1245 4594 Fragments arranged by size: 4594 1245 345 SbfI CC_TGCA'GG Cuts at: 2151 3536 2151 Size: 1385 4799 ScaI AGT'ACT Cuts at: 4432 6144 4432 Size: 1712 4472 SfcI C'TryA_G Cuts at: 826 1045 2147 2476 2901 3212 3323 3532 4667 Size: 219 1102 329 425 311 111 209 1135 Cuts at: 4667 5348 5539 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1102 681 425 329 311 219 209 191 111 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6184 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6184 SmaI CCC'GGG Cuts at: 1872 2702 3511 1872 Size: 830 809 4545 Fragments arranged by size: 4545 830 809 SmlI C'TyrA_G Cuts at: 820 1017 1943 4288 5189 5451 820 Size: 197 926 2345 901 262 1553 Fragments arranged by size: 2345 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2389 3975 493 Size: 1896 1586 2702 Fragments arranged by size: 2702 1896 1586 SpeI A'CTAG_T Cuts at: 152 152 Size: 6184 SphI G_CATG'C Cuts at: 1132 2713 3438 3542 1132 Size: 1581 725 104 3774 Fragments arranged by size: 3774 1581 725 104 SspI AAT'ATT Cuts at: 5 52 4108 5 Size: 47 4056 2081 Fragments arranged by size: 4056 2081 47 StuI AGG'CCT Cuts at: 1517 2486 3458 1517 Size: 969 972 4243 Fragments arranged by size: 4243 972 969 StyI C'CwwG_G Cuts at: 513 1065 2004 2343 3063 3480 3623 513 Size: 552 939 339 720 417 143 3074 Fragments arranged by size: 3074 939 720 552 417 339 143 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2550 2811 2945 4211 4228 4381 4566 Size: 1255 970 261 134 1266 17 153 185 Cuts at: 4566 5011 325 Size: 445 1498 Fragments arranged by size: 1498 1266 1255 970 445 261 185 153 134 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4430 6142 96 Size: 274 80 33 51 1443 2453 1712 138 Fragments arranged by size: 2453 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2929 2961 6144 6176 2929 Size: 32 3183 32 2937 Fragments arranged by size: 3183 2937 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2378 1316 Size: 1062 5122 XbaI T'CTAG_A Cuts at: 3520 3520 Size: 6184 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2976 2976 Size: 6184 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6184 XmaI C'CCGG_G Cuts at: 1870 2700 3509 1870 Size: 830 809 4545 Fragments arranged by size: 4545 830 809 XmnI GAAnn'nnTTC Cuts at: 4313 4313 Size: 6184 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2380 2415 3367 3992 Size: 53 83 186 936 846 35 952 625 Cuts at: 3992 276 Size: 2468 Fragments arranged by size: 2468 952 936 846 625 186 83 53 35 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI BstZ17I Bsu36I EcoRV FseI FspAI KasI NarI NruI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI