(Linear) (Six-base) MAPSORT of: KIEE4473.seq Check: 7480 from: 1 to: 1479 KIEE4473.orf With 182 enzymes: SgfI * March 22, 2012 18:08 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 733 874 1214 1479 Size: 733 141 340 265 Fragments arranged by size: 733 340 265 141 AatII G_ACGT'C Cuts at: 0 377 412 1364 1479 Size: 377 35 952 115 Fragments arranged by size: 952 377 115 35 AccI GT'mk_AC Cuts at: 0 277 1479 Size: 277 1202 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 67 1479 Size: 67 1412 AfeI AGC'GCT Cuts at: 0 166 1324 1479 Size: 166 1158 155 Fragments arranged by size: 1158 166 155 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1141 1405 1479 Size: 1141 264 74 Fragments arranged by size: 1141 264 74 AlwNI CAG_nnn'CTG Cuts at: 0 87 470 1479 Size: 87 383 1009 Fragments arranged by size: 1009 383 87 ApaLI G'TGCA_C Cuts at: 0 1126 1479 Size: 1126 353 AvaI C'yCGr_G Cuts at: 0 695 1479 Size: 695 784 BanI G'GyrC_C Cuts at: 0 51 952 1202 1479 Size: 51 901 250 277 Fragments arranged by size: 901 277 250 51 BanII G_rGCy'C Cuts at: 0 783 937 1175 1479 Size: 783 154 238 304 Fragments arranged by size: 783 304 238 154 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1309 1343 1479 Size: 1309 34 136 Fragments arranged by size: 1309 136 34 BfrBI ATG'CAT Cuts at: 0 1433 1479 Size: 1433 46 BglI GCCn_nnn'nGGC Cuts at: 0 1098 1479 Size: 1098 381 Bme1580I G_kGCm'C Cuts at: 0 293 1130 1406 1479 Size: 293 837 276 73 Fragments arranged by size: 837 293 276 73 BmgBI CAC'GTC Cuts at: 0 1125 1134 1479 Size: 1125 9 345 Fragments arranged by size: 1125 345 9 BmrI ACTGGGnnnn_n' Cuts at: 0 225 278 1479 Size: 225 53 1201 Fragments arranged by size: 1201 225 53 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 588 595 1033 1479 Size: 588 7 438 446 Fragments arranged by size: 588 446 438 7 Bpu10I CC'TnA_GC Cuts at: 0 1039 1479 Size: 1039 440 BsaAI yAC'GTr Cuts at: 0 384 1479 Size: 384 1095 BsaHI Gr'CG_yC Cuts at: 0 374 409 446 1055 1361 1479 Size: 374 35 37 609 306 118 Fragments arranged by size: 609 374 306 118 37 35 BsaWI w'CCGG_w Cuts at: 0 13 1479 Size: 13 1466 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 676 706 924 954 1479 Size: 676 30 218 30 525 Fragments arranged by size: 676 525 218 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 169 421 1030 1369 1479 Size: 169 252 609 339 110 Fragments arranged by size: 609 339 252 169 110 BseYI C'CCAG_C Cuts at: 0 30 1479 Size: 30 1449 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 139 156 1181 1479 Size: 139 17 1025 298 Fragments arranged by size: 1025 298 139 17 BsiEI CG_ry'CG Cuts at: 0 792 1479 Size: 792 687 BsiHKAI G_wGCw'C Cuts at: 0 347 683 783 937 1130 1226 1479 Size: 347 336 100 154 193 96 253 Fragments arranged by size: 347 336 253 193 154 100 96 Bsp1286I G_dGCh'C Cuts at: 0 293 347 683 783 937 1130 1175 1226 Size: 293 54 336 100 154 193 45 51 Cuts at: 1226 1406 1479 Size: 180 73 Fragments arranged by size: 336 293 193 180 154 100 73 54 51 45 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 733 874 1214 1405 1479 Size: 733 141 340 191 74 Fragments arranged by size: 733 340 191 141 74 BsrBI CCG'CTC Cuts at: 0 96 1043 1479 Size: 96 947 436 Fragments arranged by size: 947 436 96 BsrFI r'CCGG_y Cuts at: 0 77 124 1479 Size: 77 47 1355 Fragments arranged by size: 1355 77 47 BstAPI GCAn_nnn'nTGC Cuts at: 0 470 1479 Size: 470 1009 BstBI TT'CG_AA Cuts at: 0 506 1479 Size: 506 973 BstXI CCAn_nnnn'nTGG Cuts at: 0 321 971 1479 Size: 321 650 508 Fragments arranged by size: 650 508 321 BstYI r'GATC_y Cuts at: 0 329 1479 Size: 329 1150 BtgI C'CryG_G Cuts at: 0 365 1058 1229 1479 Size: 365 693 171 250 Fragments arranged by size: 693 365 250 171 BtsI GCAGTG_nn' Cuts at: 0 471 744 893 929 1193 1479 Size: 471 273 149 36 264 286 Fragments arranged by size: 471 286 273 264 149 36 ClaI AT'CG_AT Cuts at: 0 257 1479 Size: 257 1222 DraIII CAC_nnn'GTG Cuts at: 0 1134 1479 Size: 1134 345 DrdI GACnn_nn'nnGTC Cuts at: 0 184 1060 1479 Size: 184 876 419 Fragments arranged by size: 876 419 184 EaeI y'GGCC_r Cuts at: 0 8 1104 1479 Size: 8 1096 375 Fragments arranged by size: 1096 375 8 EarI CTCTTCn'nnn_ Cuts at: 0 196 679 1412 1479 Size: 196 483 733 67 Fragments arranged by size: 733 483 196 67 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 184 1294 1479 Size: 184 1110 185 Fragments arranged by size: 1110 185 184 EcoICRI GAG'CTC Cuts at: 0 781 935 1479 Size: 781 154 544 Fragments arranged by size: 781 544 154 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 67 588 595 1033 1479 Size: 67 521 7 438 446 Fragments arranged by size: 521 446 438 67 7 EcoNI CCTnn'n_nnAGG Cuts at: 0 1267 1479 Size: 1267 212 EcoO109I rG'GnC_Cy Cuts at: 0 186 1272 1314 1479 Size: 186 1086 42 165 Fragments arranged by size: 1086 186 165 42 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 363 1479 Size: 363 1116 FspI TGC'GCA Cuts at: 0 1192 1479 Size: 1192 287 HaeII r_GCGC'y Cuts at: 0 64 87 168 1326 1479 Size: 64 23 81 1158 153 Fragments arranged by size: 1158 153 81 64 23 HincII GTy'rAC Cuts at: 0 264 278 450 513 1479 Size: 264 14 172 63 966 Fragments arranged by size: 966 264 172 63 14 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 644 881 1479 Size: 644 237 598 Fragments arranged by size: 644 598 237 MscI TGG'CCA Cuts at: 0 1106 1479 Size: 1106 373 MslI CAynn'nnrTG Cuts at: 0 319 1123 1479 Size: 319 804 356 Fragments arranged by size: 804 356 319 MspA1I CmG'CkG Cuts at: 0 57 461 1379 1479 Size: 57 404 918 100 Fragments arranged by size: 918 404 100 57 NaeI GCC'GGC Cuts at: 0 126 1479 Size: 126 1353 NcoI C'CATG_G Cuts at: 0 1058 1479 Size: 1058 421 NgoMIV G'CCGG_C Cuts at: 0 124 1479 Size: 124 1355 NsiI A_TGCA'T Cuts at: 0 1435 1479 Size: 1435 44 NspI r_CATG'y Cuts at: 0 708 987 1433 1479 Size: 708 279 446 46 Fragments arranged by size: 708 446 279 46 PflMI CCAn_nnn'nTGG Cuts at: 0 1113 1263 1479 Size: 1113 150 216 Fragments arranged by size: 1113 216 150 PfoI T'CCnGG_A Cuts at: 0 1262 1479 Size: 1262 217 PpuMI rG'GwC_Cy Cuts at: 0 186 1314 1479 Size: 186 1128 165 Fragments arranged by size: 1128 186 165 PstI C_TGCA'G Cuts at: 0 146 475 900 1211 1322 1479 Size: 146 329 425 311 111 157 Fragments arranged by size: 425 329 311 157 146 111 SacI G_AGCT'C Cuts at: 0 783 937 1479 Size: 783 154 542 Fragments arranged by size: 783 542 154 SalI G'TCGA_C Cuts at: 0 276 1479 Size: 276 1203 SbfI CC_TGCA'GG Cuts at: 0 146 1479 Size: 146 1333 SfcI C'TryA_G Cuts at: 0 142 471 896 1207 1318 1479 Size: 142 329 425 311 111 161 Fragments arranged by size: 425 329 311 161 142 111 SmaI CCC'GGG Cuts at: 0 697 1479 Size: 697 782 SnaBI TAC'GTA Cuts at: 0 384 1479 Size: 384 1095 SphI G_CATG'C Cuts at: 0 708 1433 1479 Size: 708 725 46 Fragments arranged by size: 725 708 46 StuI AGG'CCT Cuts at: 0 481 1453 1479 Size: 481 972 26 Fragments arranged by size: 972 481 26 StyI C'CwwG_G Cuts at: 0 338 1058 1479 Size: 338 720 421 Fragments arranged by size: 720 421 338 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 545 806 940 1479 Size: 545 261 134 539 Fragments arranged by size: 545 539 261 134 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 924 956 1479 Size: 924 32 523 Fragments arranged by size: 924 523 32 Tth111I GACn'n_nGTC Cuts at: 0 373 1479 Size: 373 1106 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 971 1479 Size: 971 508 XmaI C'CCGG_G Cuts at: 0 695 1479 Size: 695 784 ZraI GAC'GTC Cuts at: 0 375 410 1362 1479 Size: 375 35 952 117 Fragments arranged by size: 952 375 117 35 Enzymes that do cut: AarI AatII AccI AcuI AfeI AlfI AlwNI ApaLI AvaI BanI BanII BcgI BfrBI BglI Bme1580I BmgBI BmrI BpmI Bpu10I BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BstAPI BstBI BstXI BstYI BtgI BtsI ClaI DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI HaeII HincII MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NsiI NspI PflMI PfoI PpuMI PstI SacI SalI SbfI SfcI SmaI SnaBI SphI StuI StyI TaqII TstI Tth111I XcmI XmaI ZraI Enzymes that do not cut: Acc65I AclI AflII AflIII AgeI AhdI AleI AloI ApaI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbsI BbvCI BciVI BclI BglII BlpI BmtI BplI BpuEI BsaI BsaBI BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrGI BssHII BssSI BstEII BstZ17I Bsu36I BtgZI CspCI DraI EagI EcoRI EcoRV FseI FspAI Hin4I HindIII HpaI KasI KpnI MfeI MluI NarI NdeI NheI NotI NruI PacI PasI PciI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacII SanDI SapI ScaI SexAI SfiI SfoI SgfI SgrAI SmlI SpeI SrfI SspI SwaI TatI XbaI XhoI XmnI