Restriction map for
pFN21AE6717
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# NO CUT ENZYMES ( 124 enzymes) #
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Acc65I AccI AccIII AclI AflII
AflIII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AvaI AviII
AvrII BbeI BbrPI BbvCI BciVI
BclI BcnI BfrI BglII BlnI
BlpI Bpu1102I BsaAI BsaWI BseAI
BseRI BsiEI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BsrFI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRV FbaI FseI FspI
HincII HindII HpaI KasI KpnI
KspI MfeI MluI MroI MunI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI Sse8387I SspBI
SspI SwaI Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AlwNI 625 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1275 CCTTAGG CC^T[ACGT]AGG
BamHI 1183 GGATCC G^GATCC
BanI 886 GGTGCC G^G[CT][GA]CC
BbsI 1477 GAAGAC GAAGAC(2/6)
BcgI 1529 GCACTCAGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 24 GCCCAAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 1477 GAAGAC GAAGAC(2/6)
BsaBI 82 GATGTCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 637 GGTCTC GGTCTC(1/5)
BsgI 467 CTGCAC GTGCAG(16/14)
BspMI 20 ACCTGC ACCTGC(4/8)
BsrBI 1857 GAGCGG CCGCTC(-3/-3)
BsrDI 856 CATTGC GCAATG(2/0)
BssSI 1537 CTCGTG CACGAG(-5/-1)
BstAPI 684 GCACTGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 1183 GGATCC [GA]^GATC[CT]
Bsu36I 1275 CCTTAGG CC^T[ACGT]AGG
CfoI 1754 GCGC GCG^C
DraII 1796 AGGTCCC [GA]G^G[ACGT]CC[CT]
DsaI 176 CCATGG C^C[GA][CT]GG
Eco81I 1275 CCTTAGG CC^T[ACGT]AGG
EcoNI 21 CCTGCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1796 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoT22I 1013 ATGCAT ATGCA^T
HaeII 1753 AGCGCC [GA]GCGC^[CT]
HapII 34 CCGG C^CGG
HgaI 724 GACGC GACGC(5/10)
HhaI 1754 GCGC GCG^C
HinP1I 1754 GCGC G^CGC
HpaII 34 CCGG C^CGG
MamI 82 GATGTCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1183 GGATCC [GA]^GATC[CT]
MspI 34 CCGG C^CGG
NcoI 176 CCATGG C^CATGG
NsiI 1013 ATGCAT ATGCA^T
NspI 566 GCATGC [GA]CATG^[CT]
PpuMI 1796 AGGTCCC [GA]G^G[AT]CC[CT]
SmlI 1698 CTTGAG C^T[CT][GA]AG
SpeI 750 ACTAGT A^CTAGT
SphI 566 GCATGC GCATG^C
XhoII 1183 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AatII 660 GACGTC GACGT^C
667 GACGTC
AccII 593 CGCG CG^CG
726 CGCG
AcyI 660 GACGTC G[GA]^CG[CT]C
667 GACGTC
BalI 741 TGGCCA TGG^CCA
949 TGGCCA
BsaHI 660 GACGTC G[GA]^CG[CT]C
667 GACGTC
BsiYI 21 CCTGCCCAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
2041 CCCAGTGGTGG
BslI 21 CCTGCCCAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
2041 CCCAGTGGTGG
BsmAI 1332 GAGAC GTCTC(1/5)
638 GTCTC
BspHI 65 TCATGA T^CATGA
1020 TCATGA
BstUI 593 CGCG CG^CG
726 CGCG
BstXI 1034 CCATAAGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1482 CCAAGGAAGTGG
EaeI 741 TGGCCA [CT]^GGCC[GA]
949 TGGCCA
EarI 289 GAAGAG CTCTTC(1/4)
1981 GAAGAG
EcoRI 326 GAATTC G^AATTC
939 GAATTC
Hin1I 660 GACGTC G[GA]^CG[CT]C
667 GACGTC
HindIII 1564 AAGCTT A^AGCTT
2151 AAGCTT
Ksp632I 289 GAAGAG CTCTTC(1/4)
1981 GAAGAG
MaeII 661 ACGT A^CGT
668 ACGT
MluNI 741 TGGCCA TGG^CCA
949 TGGCCA
MscI 741 TGGCCA TGG^CCA
949 TGGCCA
MslI 802 CATCCCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1035 CATAAGTATG
MspA1I 121 CAGCTG C[AC]G^C[GT]G
1226 CAGCTG
MvnI 593 CGCG CG^CG
726 CGCG
PstI 1342 CTGCAG CTGCA^G
2077 CTGCAG
PvuII 121 CAGCTG CAG^CTG
1226 CAGCTG
RcaI 65 TCATGA T^CATGA
1020 TCATGA
SacI 148 GAGCTC GAGCT^C
1671 GAGCTC
StuI 651 AGGCCT AGG^CCT
731 AGGCCT
TfiI 796 GAATC G^A[AT]TC
1005 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AspHI AvaII BanII
BbvI BfaI BmyI BpmI BsaJI
BsiHKAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HinfI HphI ItaI
MaeI MaeIII MboI MboII MnlI
MseI MvaI MwoI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI StyI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XmnI