(Circular) (Six-base) MAPSORT of: pFN21AE6717.seq Check: 9450 from: 1 to: 6919 pFN21AE6717 6919 bp With 182 enzymes: SgfI * March 18, 2015 14:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2669 2676 4729 278 Size: 53 83 186 936 1133 7 2053 2468 Fragments arranged by size: 2468 2053 1133 936 186 83 53 7 AccI GT'mk_AC Cuts at: 1937 4262 1937 Size: 2325 4594 Acc65I G'GTAC_C Cuts at: 4240 4240 Size: 6919 AclI AA'CG_TT Cuts at: 1563 5046 5419 1563 Size: 3483 373 3063 Fragments arranged by size: 3483 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2645 2804 3501 3557 3842 3860 4982 6366 Size: 1013 159 697 56 285 18 1122 1384 Cuts at: 6366 6634 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1122 1013 697 285 268 159 56 18 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6722 AflIII A'CryG_T Cuts at: 1203 5818 6795 1203 Size: 4615 977 1327 Fragments arranged by size: 4615 1327 977 AgeI A'CCGG_T Cuts at: 5750 5750 Size: 6919 AhdI GACnn_n'nnGTC Cuts at: 5648 5648 Size: 6919 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6919 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2588 1184 Size: 1404 5515 AlwNI CAG_nnn'CTG Cuts at: 2635 4662 6234 2635 Size: 2027 1572 3320 Fragments arranged by size: 3320 2027 1572 ApaLI G'TGCA_C Cuts at: 4976 6132 4976 Size: 1156 5763 ApoI r'AATT_y Cuts at: 1493 2331 2508 2531 2944 3637 4228 4454 4606 Size: 838 177 23 413 693 591 226 152 Cuts at: 4606 6715 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 838 693 591 413 226 177 152 23 AseI AT'TA_AT Cuts at: 160 1032 5473 160 Size: 872 4441 1606 Fragments arranged by size: 4441 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6919 AvaI C'yCGr_G Cuts at: 1870 1943 4244 1870 Size: 73 2301 4545 Fragments arranged by size: 4545 2301 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4112 4145 4112 Size: 33 6886 BamHI G'GATC_C Cuts at: 3188 4249 4616 3188 Size: 1061 367 5491 Fragments arranged by size: 5491 1061 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2891 4240 5695 618 Size: 325 217 627 1104 1349 1455 1842 Fragments arranged by size: 1842 1455 1349 1104 627 325 217 BanII G_rGCy'C Cuts at: 1391 2157 3112 3680 4238 1391 Size: 766 955 568 558 4072 Fragments arranged by size: 4072 955 766 568 558 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3489 928 Size: 971 1590 4358 Fragments arranged by size: 4358 1590 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3523 3557 5110 5144 1903 Size: 34 1586 34 1553 34 3678 Fragments arranged by size: 3678 1586 1553 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4811 6021 4811 Size: 1210 5709 BclI T'GATC_A Cuts at: 1548 1548 Size: 6919 BfrBI ATG'CAT Cuts at: 3020 3020 Size: 6919 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2035 5530 136 Size: 107 122 71 631 605 363 3495 1525 Fragments arranged by size: 3495 1525 631 605 363 122 107 71 BglII A'GATC_T Cuts at: 6914 6914 Size: 6919 BlpI GC'TnA_GC Cuts at: 4336 4336 Size: 6919 Bme1580I G_kGCm'C Cuts at: 1790 3699 4980 6136 1790 Size: 1909 1281 1156 2573 Fragments arranged by size: 2573 1909 1281 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6919 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4041 4650 5608 Size: 416 53 83 399 24 2624 609 958 Cuts at: 5608 442 Size: 1753 Fragments arranged by size: 2624 1753 958 609 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6919 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3360 3360 Size: 6919 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2171 2864 2884 3683 3785 5579 1239 Size: 932 693 20 799 102 1794 2579 Fragments arranged by size: 2579 1794 932 799 693 102 20 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3724 5044 5909 6207 3724 Size: 1320 865 298 4436 Fragments arranged by size: 4436 1320 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2648 5582 882 Size: 617 1149 2934 2219 Fragments arranged by size: 2934 2219 1149 617 BsaAI yAC'GTr Cuts at: 493 1204 4710 493 Size: 711 3506 2702 Fragments arranged by size: 3506 2702 711 BsaBI GATnn'nnATC Cuts at: 2091 6913 2091 Size: 4822 2097 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2666 2673 4726 5108 Size: 53 83 186 936 1133 7 2053 382 Cuts at: 5108 5743 275 Size: 635 1451 Fragments arranged by size: 2053 1451 1133 936 635 382 186 83 53 7 BsaWI w'CCGG_w Cuts at: 5352 5750 6024 6171 6582 5352 Size: 398 274 147 411 5689 Fragments arranged by size: 5689 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6694 Fragments arranged by size: 6694 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6919 BseYI C'CCAG_C Cuts at: 3229 6122 6618 3229 Size: 2893 496 3530 Fragments arranged by size: 3530 2893 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2457 1163 Size: 573 721 5625 Fragments arranged by size: 5625 721 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4369 5130 6158 1540 Size: 275 186 2368 761 1028 2301 Fragments arranged by size: 2368 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2157 3680 3838 4980 5065 6136 2157 Size: 1523 158 1142 85 1071 2940 Fragments arranged by size: 2940 1523 1142 1071 158 85 BsmI GAATG_Cn' Cuts at: 4430 4523 4430 Size: 93 6826 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2157 3112 3680 3699 3838 4238 4980 Size: 399 367 955 568 19 139 400 742 Cuts at: 4980 5065 6136 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 955 742 568 400 399 367 139 85 19 BspEI T'CCGG_A Cuts at: 6582 6582 Size: 6919 BspHI T'CATG_A Cuts at: 2070 3025 4808 6538 2070 Size: 955 1783 1730 2451 Fragments arranged by size: 2451 1783 1730 955 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2034 4274 844 Size: 1190 2240 3489 Fragments arranged by size: 3489 2240 1190 BsrBI CCG'CTC Cuts at: 1394 1823 3864 4806 1394 Size: 429 2041 942 3507 Fragments arranged by size: 3507 2041 942 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2860 5414 5588 65 Size: 1183 1612 2554 174 1396 Fragments arranged by size: 2554 1612 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5563 5750 1700 Size: 110 3753 187 2869 Fragments arranged by size: 3753 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6919 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6919 BssSI C'ACGA_G Cuts at: 3542 4979 5991 3542 Size: 1437 1012 4470 Fragments arranged by size: 4470 1437 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2695 794 Size: 1901 5018 BstBI TT'CG_AA Cuts at: 5723 5723 Size: 6919 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6919 BstXI CCAn_nnnn'nTGG Cuts at: 3046 3494 3046 Size: 448 6471 BstYI r'GATC_y Cuts at: 1972 3188 4249 4616 5001 5018 6470 6556 6568 Size: 1216 1061 367 385 17 1452 86 12 Cuts at: 6568 6914 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1216 1061 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3281 3281 Size: 6919 BtgI C'CryG_G Cuts at: 513 1065 2004 2181 513 Size: 552 939 177 5251 Fragments arranged by size: 5251 939 552 177 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3311 487 Size: 683 56 2085 4095 Fragments arranged by size: 4095 2085 683 56 BtsI GCAGTG_nn' Cuts at: 2689 3080 3908 4435 5229 5249 2689 Size: 391 828 527 794 20 4359 Fragments arranged by size: 4359 828 794 527 391 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6919 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3498 3533 529 Size: 35 2934 35 3915 Fragments arranged by size: 3915 2934 35 35 DraI TTT'AAA Cuts at: 4223 4223 Size: 6919 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5926 809 Size: 507 4610 1802 Fragments arranged by size: 4610 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2746 2954 4366 Size: 54 1425 50 275 18 916 208 1412 Cuts at: 4366 5255 6884 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1412 916 889 275 208 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4366 1537 Size: 275 2554 4090 Fragments arranged by size: 4090 2554 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2289 3981 4849 1353 Size: 936 1692 868 3423 Fragments arranged by size: 3423 1692 936 868 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5506 5880 6026 387 Size: 1064 138 3917 374 146 1280 Fragments arranged by size: 3917 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2155 3678 2155 Size: 1523 5396 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2171 2645 2804 2864 2884 3501 3557 Size: 393 539 474 159 60 20 617 56 Cuts at: 3557 3683 3785 3842 3860 4982 5579 6366 6634 Size: 126 102 57 18 1122 597 787 268 Cuts at: 6634 1239 Size: 1524 Fragments arranged by size: 1524 1122 787 617 597 539 474 393 268 159 126 102 60 57 56 20 18 EcoNI CCTnn'n_nnAGG Cuts at: 2030 2030 Size: 6919 EcoO109I rG'GnC_Cy Cuts at: 3802 3802 Size: 6919 EcoRI G'AATT_C Cuts at: 2331 2944 4228 4606 2331 Size: 613 1284 378 4644 Fragments arranged by size: 4644 1284 613 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3163 3221 3516 3753 3163 Size: 58 295 237 6329 Fragments arranged by size: 6329 295 237 58 FspI TGC'GCA Cuts at: 5425 5425 Size: 6919 HaeII r_GCGC'y Cuts at: 1678 3762 6066 1678 Size: 2084 2304 2531 Fragments arranged by size: 2531 2304 2084 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2304 2336 2880 2912 5560 Size: 32 135 32 732 32 544 32 2648 Cuts at: 5560 5592 5634 5666 1373 Size: 32 42 32 2626 Fragments arranged by size: 2648 2626 732 544 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4263 4509 669 Size: 988 281 2325 246 3079 Fragments arranged by size: 3079 2325 988 281 246 HindIII A'AGCT_T Cuts at: 748 3569 4156 748 Size: 2821 587 3511 Fragments arranged by size: 3511 2821 587 HpaI GTT'AAC Cuts at: 4509 4509 Size: 6919 KpnI G_GTAC'C Cuts at: 4244 4244 Size: 6919 MfeI C'AATT_G Cuts at: 4518 4518 Size: 6919 MluI A'CGCG_T Cuts at: 6795 6795 Size: 6919 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6033 6217 1305 Size: 4728 184 2007 Fragments arranged by size: 4728 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2748 2956 10 Size: 54 1425 343 916 208 3973 Fragments arranged by size: 3973 1425 916 343 208 54 MslI CAynn'nnrTG Cuts at: 518 1166 2811 3044 4877 5236 5395 518 Size: 648 1645 233 1833 359 159 2042 Fragments arranged by size: 2042 1833 1645 648 359 233 159 MspA1I CmG'CkG Cuts at: 1606 2128 3233 4335 5012 6160 6405 1606 Size: 522 1105 1102 677 1148 245 2120 Fragments arranged by size: 2120 1148 1105 1102 677 522 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2181 513 Size: 552 939 177 5251 Fragments arranged by size: 5251 939 552 177 NdeI CA'TA_TG Cuts at: 387 387 Size: 6919 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6919 NotI GC'GGCC_GC Cuts at: 4366 4366 Size: 6919 NsiI A_TGCA'T Cuts at: 3022 3022 Size: 6919 NspI r_CATG'y Cuts at: 1132 1229 2575 4277 5822 1132 Size: 97 1346 1702 1545 2229 Fragments arranged by size: 2229 1702 1545 1346 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6919 PciI A'CATG_T Cuts at: 5818 5818 Size: 6919 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6919 PfoI T'CCnGG_A Cuts at: 2841 2923 3216 3720 2841 Size: 82 293 504 6040 Fragments arranged by size: 6040 504 293 82 PmeI GTTT'AAAC Cuts at: 4223 4223 Size: 6919 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2283 2315 4988 5020 6501 6533 2283 Size: 32 2673 32 1481 32 2669 Fragments arranged by size: 2673 2669 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3802 3802 Size: 6919 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6919 PsiI TTA'TAA Cuts at: 4489 4489 Size: 6919 PstI C_TGCA'G Cuts at: 830 3351 4086 4271 830 Size: 2521 735 185 3478 Fragments arranged by size: 3478 2521 735 185 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6919 PvuII CAG'CTG Cuts at: 2128 3233 2128 Size: 1105 5814 SacI G_AGCT'C Cuts at: 2157 3680 2157 Size: 1523 5396 SalI G'TCGA_C Cuts at: 1936 4261 1936 Size: 2325 4594 SbfI CC_TGCA'GG Cuts at: 4271 4271 Size: 6919 ScaI AGT'ACT Cuts at: 2754 3941 4106 5167 6879 2754 Size: 1187 165 1061 1712 2794 Fragments arranged by size: 2794 1712 1187 1061 165 SfcI C'TryA_G Cuts at: 826 1045 2110 3347 4082 4267 5402 6083 6274 Size: 219 1065 1237 735 185 1135 681 191 Cuts at: 6274 826 Size: 1471 Fragments arranged by size: 1471 1237 1135 1065 735 681 219 191 185 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6919 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6919 SmaI CCC'GGG Cuts at: 1872 4246 1872 Size: 2374 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3703 5023 5924 6186 820 Size: 197 926 1760 1320 901 262 1553 Fragments arranged by size: 1760 1553 1320 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4710 493 Size: 4217 2702 SpeI A'CTAG_T Cuts at: 152 2755 152 Size: 2603 4316 SphI G_CATG'C Cuts at: 1132 2575 4277 1132 Size: 1443 1702 3774 Fragments arranged by size: 3774 1702 1443 SspI AAT'ATT Cuts at: 5 52 4843 5 Size: 47 4791 2081 Fragments arranged by size: 4791 2081 47 StuI AGG'CCT Cuts at: 1517 2658 2738 1517 Size: 1141 80 5698 Fragments arranged by size: 5698 1141 80 StyI C'CwwG_G Cuts at: 513 1065 2004 2031 2181 3417 3469 3487 4191 Size: 552 939 27 150 1236 52 18 704 Cuts at: 4191 4358 513 Size: 167 3074 Fragments arranged by size: 3074 1236 939 704 552 167 150 52 27 18 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2406 3482 4031 4946 4963 5116 5301 Size: 1255 826 1076 549 915 17 153 185 Cuts at: 5301 5746 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1076 915 826 549 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2752 2965 3939 Size: 274 80 33 51 1443 775 213 974 Cuts at: 3939 4104 5165 6877 96 Size: 165 1061 1712 138 Fragments arranged by size: 1712 1443 1061 974 775 274 213 165 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3059 3091 6879 6911 3059 Size: 32 3788 32 3067 Fragments arranged by size: 3788 3067 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6919 XbaI T'CTAG_A Cuts at: 4255 4255 Size: 6919 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6919 XmaI C'CCGG_G Cuts at: 1870 4244 1870 Size: 2374 4545 XmnI GAAnn'nnTTC Cuts at: 2298 2331 2658 5048 2298 Size: 33 327 2390 4169 Fragments arranged by size: 4169 2390 327 33 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2667 2674 4727 276 Size: 53 83 186 936 1133 7 2053 2468 Fragments arranged by size: 2468 2053 1133 936 186 83 53 7 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI