Restriction map for
pFN21AE6717
(Linear) (Six-base) MAPSORT of: KIEE6717.seq Check: 9035 from: 1 to: 2214
KIEE6717.orf
With 182 enzymes: SgfI *
March 16, 2015 11:36 ..
AatII G_ACGT'C
Cuts at: 0 664 671 2214
Size: 664 7 1543
Fragments arranged by size:
1543 664 7
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 640 799 1496 1552 1837 1855 2214
Size: 640 159 697 56 285 18 359
Fragments arranged by size:
697 640 359 285 159 56 18
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 583 2214
Size: 583 1631
AlwNI CAG_nnn'CTG
Cuts at: 0 630 2214
Size: 630 1584
ApoI r'AATT_y
Cuts at: 0 326 503 526 939 1632 2214
Size: 326 177 23 413 693 582
Fragments arranged by size:
693 582 413 326 177 23
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2107 2140 2214
Size: 2107 33 74
Fragments arranged by size:
2107 74 33
BamHI G'GATC_C
Cuts at: 0 1183 2214
Size: 1183 1031
BanI G'GyrC_C
Cuts at: 0 886 2214
Size: 886 1328
BanII G_rGCy'C
Cuts at: 0 152 1107 1675 2214
Size: 152 955 568 539
Fragments arranged by size:
955 568 539 152
BbsI GAAGACnn'nnnn_
Cuts at: 0 1484 2214
Size: 1484 730
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1518 1552 2214
Size: 1518 34 662
Fragments arranged by size:
1518 662 34
BfrBI ATG'CAT
Cuts at: 0 1015 2214
Size: 1015 1199
BglI GCCn_nnn'nGGC
Cuts at: 0 30 2214
Size: 30 2184
Bme1580I G_kGCm'C
Cuts at: 0 1694 2214
Size: 1694 520
BmrI ACTGGGnnnn_n'
Cuts at: 0 2036 2214
Size: 2036 178
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1355 2214
Size: 1355 859
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 859 879 1678 1780 2214
Size: 166 693 20 799 102 434
Fragments arranged by size:
799 693 434 166 102 20
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1719 2214
Size: 1719 495
BsaI GGTCTCn'nnnn_
Cuts at: 0 643 2214
Size: 643 1571
BsaBI GATnn'nnATC
Cuts at: 0 86 2214
Size: 86 2128
BsaHI Gr'CG_yC
Cuts at: 0 661 668 2214
Size: 661 7 1546
Fragments arranged by size:
1546 661 7
BseYI C'CCAG_C
Cuts at: 0 1224 2214
Size: 1224 990
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 452 2214
Size: 452 1762
BsiHKAI G_wGCw'C
Cuts at: 0 152 1675 1833 2214
Size: 152 1523 158 381
Fragments arranged by size:
1523 381 158 152
Bsp1286I G_dGCh'C
Cuts at: 0 152 1107 1675 1694 1833 2214
Size: 152 955 568 19 139 381
Fragments arranged by size:
955 568 381 152 139 19
BspHI T'CATG_A
Cuts at: 0 65 1020 2214
Size: 65 955 1194
Fragments arranged by size:
1194 955 65
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 29 2214
Size: 29 2185
BsrBI CCG'CTC
Cuts at: 0 1859 2214
Size: 1859 355
BsrDI GCAATG_nn'
Cuts at: 0 855 2214
Size: 855 1359
BssSI C'ACGA_G
Cuts at: 0 1537 2214
Size: 1537 677
BstAPI GCAn_nnn'nTGC
Cuts at: 0 690 2214
Size: 690 1524
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1041 1489 2214
Size: 1041 448 725
Fragments arranged by size:
1041 725 448
BstYI r'GATC_y
Cuts at: 0 1183 2214
Size: 1183 1031
Bsu36I CC'TnA_GG
Cuts at: 0 1276 2214
Size: 1276 938
BtgI C'CryG_G
Cuts at: 0 176 2214
Size: 176 2038
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1306 2214
Size: 1306 908
BtsI GCAGTG_nn'
Cuts at: 0 684 1075 1903 2214
Size: 684 391 828 311
Fragments arranged by size:
828 684 391 311
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1493 1528 2214
Size: 1493 35 686
Fragments arranged by size:
1493 686 35
EaeI y'GGCC_r
Cuts at: 0 741 949 2214
Size: 741 208 1265
Fragments arranged by size:
1265 741 208
EarI CTCTTCn'nnn_
Cuts at: 0 284 1976 2214
Size: 284 1692 238
Fragments arranged by size:
1692 284 238
EcoICRI GAG'CTC
Cuts at: 0 150 1673 2214
Size: 150 1523 541
Fragments arranged by size:
1523 541 150
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 640 799 859 879 1496 1552 1678
Size: 166 474 159 60 20 617 56 126
Cuts at: 1678 1780 1837 1855 2214
Size: 102 57 18 359
Fragments arranged by size:
617 474 359 166 159 126 102 60
57 56 20 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 25 2214
Size: 25 2189
EcoO109I rG'GnC_Cy
Cuts at: 0 1797 2214
Size: 1797 417
EcoRI G'AATT_C
Cuts at: 0 326 939 2214
Size: 326 613 1275
Fragments arranged by size:
1275 613 326
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1158 1216 1511 1748 2214
Size: 1158 58 295 237 466
Fragments arranged by size:
1158 466 295 237 58
HaeII r_GCGC'y
Cuts at: 0 1757 2214
Size: 1757 457
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 299 331 875 907 2214
Size: 299 32 544 32 1307
Fragments arranged by size:
1307 544 299 32 32
HindIII A'AGCT_T
Cuts at: 0 1564 2151 2214
Size: 1564 587 63
Fragments arranged by size:
1564 587 63
MscI TGG'CCA
Cuts at: 0 743 951 2214
Size: 743 208 1263
Fragments arranged by size:
1263 743 208
MslI CAynn'nnrTG
Cuts at: 0 806 1039 2214
Size: 806 233 1175
Fragments arranged by size:
1175 806 233
MspA1I CmG'CkG
Cuts at: 0 123 1228 2214
Size: 123 1105 986
Fragments arranged by size:
1105 986 123
NcoI C'CATG_G
Cuts at: 0 176 2214
Size: 176 2038
NsiI A_TGCA'T
Cuts at: 0 1017 2214
Size: 1017 1197
NspI r_CATG'y
Cuts at: 0 570 2214
Size: 570 1644
PfoI T'CCnGG_A
Cuts at: 0 836 918 1211 1715 2214
Size: 836 82 293 504 499
Fragments arranged by size:
836 504 499 293 82
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 278 310 2214
Size: 278 32 1904
Fragments arranged by size:
1904 278 32
PpuMI rG'GwC_Cy
Cuts at: 0 1797 2214
Size: 1797 417
PstI C_TGCA'G
Cuts at: 0 1346 2081 2214
Size: 1346 735 133
Fragments arranged by size:
1346 735 133
PvuII CAG'CTG
Cuts at: 0 123 1228 2214
Size: 123 1105 986
Fragments arranged by size:
1105 986 123
SacI G_AGCT'C
Cuts at: 0 152 1675 2214
Size: 152 1523 539
Fragments arranged by size:
1523 539 152
ScaI AGT'ACT
Cuts at: 0 749 1936 2101 2214
Size: 749 1187 165 113
Fragments arranged by size:
1187 749 165 113
SfcI C'TryA_G
Cuts at: 0 105 1342 2077 2214
Size: 105 1237 735 137
Fragments arranged by size:
1237 735 137 105
SmlI C'TyrA_G
Cuts at: 0 1698 2214
Size: 1698 516
SpeI A'CTAG_T
Cuts at: 0 750 2214
Size: 750 1464
SphI G_CATG'C
Cuts at: 0 570 2214
Size: 570 1644
StuI AGG'CCT
Cuts at: 0 653 733 2214
Size: 653 80 1481
Fragments arranged by size:
1481 653 80
StyI C'CwwG_G
Cuts at: 0 26 176 1412 1464 1482 2186 2214
Size: 26 150 1236 52 18 704 28
Fragments arranged by size:
1236 704 150 52 28 26 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 401 1477 2026 2214
Size: 401 1076 549 188
Fragments arranged by size:
1076 549 401 188
TatI w'GTAC_w
Cuts at: 0 747 960 1934 2099 2214
Size: 747 213 974 165 115
Fragments arranged by size:
974 747 213 165 115
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1054 1086 2214
Size: 1054 32 1128
Fragments arranged by size:
1128 1054 32
XmnI GAAnn'nnTTC
Cuts at: 0 293 326 653 2214
Size: 293 33 327 1561
Fragments arranged by size:
1561 327 293 33
ZraI GAC'GTC
Cuts at: 0 662 669 2214
Size: 662 7 1545
Fragments arranged by size:
1545 662 7
Enzymes that do cut:
AatII AcuI AlfI AlwNI ApoI BaeI BamHI BanI
BanII BbsI BcgI BfrBI BglI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaBI BsaHI BseYI BsgI BsiHKAI
Bsp1286I BspHI BspMI BsrBI BsrDI BssSI BstAPI BstXI
BstYI Bsu36I BtgI BtgZI BtsI CspCI EaeI EarI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I
HindIII MscI MslI MspA1I NcoI NsiI NspI PfoI
PpiI PpuMI PstI PvuII SacI ScaI SfcI SmlI
SpeI SphI StuI StyI TaqII TatI TstI XmnI
ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BbeI BbvCI BciVI BclI BglII BlpI
BmgBI BmtI Bpu10I BsaAI BsaWI BsaXI BseRI BsiEI
BsiWI BsmI BsmBI BspEI BsrFI BsrGI BssHII BstBI
BstEII BstZ17I ClaI DraI DraIII DrdI EagI EciI
EcoRV FseI FspI FspAI HincII HpaI KasI KpnI
MfeI MluI MmeI NaeI NarI NdeI NgoMIV NheI
NotI NruI PacI PasI PciI PflMI PmeI PmlI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII
SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SrfI SspI SwaI Tth111I XbaI
XcmI XhoI XmaI