(Circular) (Six-base) MAPSORT of: pFN21AE0500.seq Check: 414 from: 1 to: 6880 pFN21AE0500 6880 bp With 182 enzymes: SgfI * February 26, 2010 09:49 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3147 4690 278 Size: 53 83 186 936 1611 1543 2468 Fragments arranged by size: 2468 1611 1543 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2976 4223 1937 Size: 1039 1247 4594 Fragments arranged by size: 4594 1247 1039 Acc65I G'GTAC_C Cuts at: 4201 4201 Size: 6880 AclI AA'CG_TT Cuts at: 1563 5007 5380 1563 Size: 3444 373 3063 Fragments arranged by size: 3444 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2009 3176 3593 4943 6327 6595 1632 Size: 377 1167 417 1350 1384 268 1917 Fragments arranged by size: 1917 1384 1350 1167 417 377 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6683 AflIII A'CryG_T Cuts at: 1203 3417 5779 6756 1203 Size: 2214 2362 977 1327 Fragments arranged by size: 2362 2214 1327 977 AgeI A'CCGG_T Cuts at: 5711 5711 Size: 6880 AhdI GACnn_n'nnGTC Cuts at: 5609 5609 Size: 6880 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6880 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3405 1184 Size: 2221 4659 AlwNI CAG_nnn'CTG Cuts at: 2477 3366 3646 4623 6195 2477 Size: 889 280 977 1572 3162 Fragments arranged by size: 3162 1572 977 889 280 ApaI G_GGCC'C Cuts at: 3592 3786 3592 Size: 194 6686 ApaLI G'TGCA_C Cuts at: 4937 6093 4937 Size: 1156 5724 ApoI r'AATT_y Cuts at: 1493 2301 2696 2717 4189 4415 4567 6676 1493 Size: 808 395 21 1472 226 152 2109 1697 Fragments arranged by size: 2109 1697 1472 808 395 226 152 21 AseI AT'TA_AT Cuts at: 160 1032 5434 160 Size: 872 4402 1606 Fragments arranged by size: 4402 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6880 AvaI C'yCGr_G Cuts at: 1870 1943 3138 4205 1870 Size: 73 1195 1067 4545 Fragments arranged by size: 4545 1195 1067 73 BamHI G'GATC_C Cuts at: 4210 4577 4210 Size: 367 6513 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2150 4201 5656 618 Size: 325 217 627 363 2051 1455 1842 Fragments arranged by size: 2051 1842 1455 627 363 325 217 BanII G_rGCy'C Cuts at: 1391 2665 3592 3773 3786 3960 3999 4199 1391 Size: 1274 927 181 13 174 39 200 4072 Fragments arranged by size: 4072 1274 927 200 181 174 39 13 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2315 3139 3879 928 Size: 971 416 824 740 3929 Fragments arranged by size: 3929 971 824 740 416 BbvCI CC'TCA_GC Cuts at: 3647 3647 Size: 6880 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5071 5105 1903 Size: 34 3134 34 3678 Fragments arranged by size: 3678 3134 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4772 5982 4772 Size: 1210 5670 BclI T'GATC_A Cuts at: 1548 1548 Size: 6880 BfrBI ATG'CAT Cuts at: 3110 3457 3110 Size: 347 6533 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5491 136 Size: 107 122 71 631 605 3819 1525 Fragments arranged by size: 3819 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3213 3478 6875 3213 Size: 265 3397 3218 Fragments arranged by size: 3397 3218 265 BlpI GC'TnA_GC Cuts at: 3767 4297 3767 Size: 530 6350 Bme1580I G_kGCm'C Cuts at: 1790 3592 3786 4941 6097 1790 Size: 1802 194 1155 1156 2573 Fragments arranged by size: 2573 1802 1156 1155 194 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6880 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2701 3780 4611 Size: 416 53 83 399 24 1284 1079 831 Cuts at: 4611 5569 442 Size: 958 1753 Fragments arranged by size: 1753 1284 1079 958 831 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 4149 1056 Size: 3093 3787 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2936 3290 3956 4052 5540 1239 Size: 1697 354 666 96 1488 2579 Fragments arranged by size: 2579 1697 1488 666 354 96 Bpu10I CC'TnA_GC Cuts at: 2376 2664 3647 2376 Size: 288 983 5609 Fragments arranged by size: 5609 983 288 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3944 4013 5005 5870 6168 3944 Size: 69 992 865 298 4656 Fragments arranged by size: 4656 992 865 298 69 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2051 3078 4028 5543 882 Size: 617 552 1027 950 1515 2219 Fragments arranged by size: 2219 1515 1027 950 617 552 BsaAI yAC'GTr Cuts at: 493 1204 4671 493 Size: 711 3467 2702 Fragments arranged by size: 3467 2702 711 BsaBI GATnn'nnATC Cuts at: 6874 6874 Size: 6880 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3144 4687 5069 5704 Size: 53 83 186 936 1611 1543 382 635 Cuts at: 5704 275 Size: 1451 Fragments arranged by size: 1611 1543 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3174 5313 5711 5985 6132 6543 3174 Size: 2139 398 274 147 411 3511 Fragments arranged by size: 3511 2139 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3869 3899 1178 Size: 30 165 30 2466 30 4159 Fragments arranged by size: 4159 2466 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2519 2858 3890 3953 1186 Size: 1333 339 1032 63 4113 Fragments arranged by size: 4113 1333 1032 339 63 BseYI C'CCAG_C Cuts at: 4068 6083 6579 4068 Size: 2015 496 4369 Fragments arranged by size: 4369 2015 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6307 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4330 5091 6119 1540 Size: 275 186 2329 761 1028 2301 Fragments arranged by size: 2329 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2665 2775 3960 3999 4941 5026 6097 2665 Size: 110 1185 39 942 85 1071 3448 Fragments arranged by size: 3448 1185 1071 942 110 85 39 BsmI GAATG_Cn' Cuts at: 2927 3112 3459 4391 4484 2927 Size: 185 347 932 93 5323 Fragments arranged by size: 5323 932 347 185 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2665 2775 3592 3773 3786 3960 3999 Size: 399 875 110 817 181 13 174 39 Cuts at: 3999 4199 4941 5026 6097 1391 Size: 200 742 85 1071 2174 Fragments arranged by size: 2174 1071 875 817 742 399 200 181 174 110 85 39 13 BspEI T'CCGG_A Cuts at: 6543 6543 Size: 6880 BspHI T'CATG_A Cuts at: 4769 6499 4769 Size: 1730 5150 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3246 4235 844 Size: 2402 989 3489 Fragments arranged by size: 3489 2402 989 BsrBI CCG'CTC Cuts at: 1394 1823 2334 3411 4767 1394 Size: 429 511 1077 1356 3507 Fragments arranged by size: 3507 1356 1077 511 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5375 5549 65 Size: 1183 4127 174 1396 Fragments arranged by size: 4127 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3522 5524 5711 1700 Size: 110 1712 2002 187 2869 Fragments arranged by size: 2869 2002 1712 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6880 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6880 BssSI C'ACGA_G Cuts at: 4940 5952 4940 Size: 1012 5868 BstAPI GCAn_nnn'nTGC Cuts at: 794 3289 3366 794 Size: 2495 77 4308 Fragments arranged by size: 4308 2495 77 BstBI TT'CG_AA Cuts at: 2326 2963 5684 2326 Size: 637 2721 3522 Fragments arranged by size: 3522 2721 637 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6880 BstXI CCAn_nnnn'nTGG Cuts at: 2011 4132 2011 Size: 2121 4759 BstYI r'GATC_y Cuts at: 1972 2058 2285 3120 3213 3478 4210 4577 4962 Size: 86 227 835 93 265 732 367 385 Cuts at: 4962 4979 6431 6517 6529 6875 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 835 732 385 367 346 265 227 93 86 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2010 3129 513 Size: 552 939 6 1119 4264 Fragments arranged by size: 4264 1119 939 552 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6141 Fragments arranged by size: 6141 683 56 BtsI GCAGTG_nn' Cuts at: 2478 4396 5190 5210 2478 Size: 1918 794 20 4148 Fragments arranged by size: 4148 1918 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6880 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6845 DraI TTT'AAA Cuts at: 3732 4184 3732 Size: 452 6428 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5887 809 Size: 507 4571 1802 Fragments arranged by size: 4571 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2823 3046 Size: 54 1425 50 275 18 177 816 223 Cuts at: 3046 4122 4327 5216 6845 8 Size: 1076 205 889 1629 43 Fragments arranged by size: 1629 1425 1076 889 816 275 223 205 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4327 1537 Size: 275 2515 4090 Fragments arranged by size: 4090 2515 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2508 2548 2616 2651 2763 3813 3867 3948 Size: 1155 40 68 35 112 1050 54 81 Cuts at: 3948 4131 4810 1353 Size: 183 679 3423 Fragments arranged by size: 3423 1155 1050 679 183 112 81 68 54 40 35 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3096 5467 5841 5987 387 Size: 1064 138 1507 2371 374 146 1280 Fragments arranged by size: 2371 1507 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2663 3958 3997 2663 Size: 1295 39 5546 Fragments arranged by size: 5546 1295 39 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2009 2936 3176 3290 3593 3956 4052 Size: 393 377 927 240 114 303 363 96 Cuts at: 4052 4943 5540 6327 6595 1239 Size: 891 597 787 268 1524 Fragments arranged by size: 1524 927 891 787 597 393 377 363 303 268 240 114 96 EcoO109I rG'GnC_Cy Cuts at: 3588 3589 3588 Size: 1 6879 EcoRI G'AATT_C Cuts at: 4189 4567 4189 Size: 378 6502 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2339 3580 2339 Size: 1241 5639 FspI TGC'GCA Cuts at: 5386 5386 Size: 6880 HaeII r_GCGC'y Cuts at: 1678 3366 3848 6027 1678 Size: 1688 482 2179 2531 Fragments arranged by size: 2531 2179 1688 482 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3869 3901 4089 4121 5521 Size: 32 135 32 2297 32 188 32 1400 Cuts at: 5521 5553 5595 5627 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2297 1400 188 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2293 3322 4224 4470 669 Size: 988 281 355 1029 902 246 3079 Fragments arranged by size: 3079 1029 988 902 355 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6880 HpaI GTT'AAC Cuts at: 3322 4470 3322 Size: 1148 5732 KpnI G_GTAC'C Cuts at: 4205 4205 Size: 6880 MfeI C'AATT_G Cuts at: 4479 4479 Size: 6880 MluI A'CGCG_T Cuts at: 3417 6756 3417 Size: 3339 3541 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2086 5994 6178 1305 Size: 781 3908 184 2007 Fragments arranged by size: 3908 2007 781 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 2825 3048 4124 10 Size: 54 1425 343 177 816 223 1076 2766 Fragments arranged by size: 2766 1425 1076 816 343 223 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2342 2393 3344 4838 5197 5356 Size: 648 843 333 51 951 1494 359 159 Cuts at: 5356 518 Size: 2042 Fragments arranged by size: 2042 1494 951 843 648 359 333 159 51 MspA1I CmG'CkG Cuts at: 1606 2411 4132 4296 4973 6121 6366 1606 Size: 805 1721 164 677 1148 245 2120 Fragments arranged by size: 2120 1721 1148 805 677 245 164 NcoI C'CATG_G Cuts at: 513 1065 2004 2010 3129 513 Size: 552 939 6 1119 4264 Fragments arranged by size: 4264 1119 939 552 6 NdeI CA'TA_TG Cuts at: 387 3043 387 Size: 2656 4224 NheI G'CTAG_C Cuts at: 1052 4145 1052 Size: 3093 3787 NotI GC'GGCC_GC Cuts at: 4327 4327 Size: 6880 NsiI A_TGCA'T Cuts at: 3112 3459 3112 Size: 347 6533 NspI r_CATG'y Cuts at: 1132 1229 2347 4238 5783 1132 Size: 97 1118 1891 1545 2229 Fragments arranged by size: 2229 1891 1545 1118 97 PasI CC'CwG_GG Cuts at: 1348 3592 1348 Size: 2244 4636 PciI A'CATG_T Cuts at: 5779 5779 Size: 6880 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6880 PmeI GTTT'AAAC Cuts at: 4184 4184 Size: 6880 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4949 4981 6462 6494 4949 Size: 32 1481 32 5335 Fragments arranged by size: 5335 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6880 PsiI TTA'TAA Cuts at: 4450 4450 Size: 6880 PspOMI G'GGCC_C Cuts at: 3588 3782 3588 Size: 194 6686 PstI C_TGCA'G Cuts at: 830 2410 2473 3309 3371 4232 830 Size: 1580 63 836 62 861 3478 Fragments arranged by size: 3478 1580 861 836 63 62 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6880 PvuII CAG'CTG Cuts at: 2411 4132 2411 Size: 1721 5159 SacI G_AGCT'C Cuts at: 2665 3960 3999 2665 Size: 1295 39 5546 Fragments arranged by size: 5546 1295 39 SalI G'TCGA_C Cuts at: 1936 4222 1936 Size: 2286 4594 SapI GCTCTTCn'nnn_ Cuts at: 2508 2548 2763 3948 4131 2508 Size: 40 215 1185 183 5257 Fragments arranged by size: 5257 1185 215 183 40 SbfI CC_TGCA'GG Cuts at: 4232 4232 Size: 6880 ScaI AGT'ACT Cuts at: 5128 6840 5128 Size: 1712 5168 SfcI C'TryA_G Cuts at: 826 1045 2406 2469 2977 3305 3367 4228 5363 Size: 219 1361 63 508 328 62 861 1135 Cuts at: 5363 6044 6235 826 Size: 681 191 1471 Fragments arranged by size: 1471 1361 1135 861 681 508 328 219 191 63 62 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6880 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6880 SmaI CCC'GGG Cuts at: 1872 3140 4207 1872 Size: 1268 1067 4545 Fragments arranged by size: 4545 1268 1067 SmlI C'TyrA_G Cuts at: 820 1017 1943 3959 3992 4984 5885 6147 820 Size: 197 926 2016 33 992 901 262 1553 Fragments arranged by size: 2016 1553 992 926 901 262 197 33 SnaBI TAC'GTA Cuts at: 493 4671 493 Size: 4178 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6880 SphI G_CATG'C Cuts at: 1132 4238 1132 Size: 3106 3774 SspI AAT'ATT Cuts at: 5 52 4804 5 Size: 47 4752 2081 Fragments arranged by size: 4752 2081 47 StuI AGG'CCT Cuts at: 1517 2458 4112 1517 Size: 941 1654 4285 Fragments arranged by size: 4285 1654 941 StyI C'CwwG_G Cuts at: 513 1065 2004 2010 3129 3867 4319 513 Size: 552 939 6 1119 738 452 3074 Fragments arranged by size: 3074 1119 939 738 552 452 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4025 4907 4924 5077 5262 5707 325 Size: 1255 2445 882 17 153 185 445 1498 Fragments arranged by size: 2445 1498 1255 882 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5126 6838 96 Size: 274 80 33 51 1443 3149 1712 138 Fragments arranged by size: 3149 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6840 6872 6840 Size: 32 6848 Tth111I GACn'n_nGTC Cuts at: 1316 3755 1316 Size: 2439 4441 XbaI T'CTAG_A Cuts at: 4216 4216 Size: 6880 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2069 2893 3056 2069 Size: 824 163 5893 Fragments arranged by size: 5893 824 163 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6880 XmaI C'CCGG_G Cuts at: 1870 3138 4205 1870 Size: 1268 1067 4545 Fragments arranged by size: 4545 1268 1067 XmnI GAAnn'nnTTC Cuts at: 2322 5009 2322 Size: 2687 4193 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3145 4688 276 Size: 53 83 186 936 1611 1543 2468 Fragments arranged by size: 2468 1611 1543 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI