################################## # NO CUT ENZYMES ( 150 enzymes) # ################################## Acc65I AccI AccIII AclI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII BalI BamHI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglII BlpI Bpu1102I BpuAI BsaBI BsaI BsaWI BseAI BsiWI BsmBI BsmFI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrGI BssSI Bst1107I BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I CelII ClaI CpoI DpnII DraI DraII DraIII DrdI DsaI Eam1105I Eco47III Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT22I FbaI FseI FspI HgaI HindIII HpaI HphI KpnI KspI MamI MboI MfeI MflI MluI MluNI MroI MscI MslI MspA1I MunI NciI NcoI NdeI NdeII NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SapI Sau3AI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI Tth111I TthHB8I Van91I XbaI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AatII 354 GACGTC GACGT^C AvrII 874 CCTAGG C^CTAGG BbeI 419 GGCGCC GGCGC^C BfaI 875 CTAG C^TAG BglI 411 GCCTGAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 874 CCTAGG C^CTAGG BpmI 292 CTGGAG CTGGAG(16/14) BsaAI 684 CACGTA [CT]AC^GT[GA] BseRI 28 GAGGAG GAGGAG(10/8) BsiEI 96 CGGCCG CG[GA][CT]^CG BsmAI 203 GAGAC GTCTC(1/5) BsmI 787 GCATTC GAATGC(1/-1) BsrFI 43 GCCGGC [GA]^CCGG[CT] BssHII 101 GCGCGC G^CGCGC BssKI 672 CCTGG ^CC[ACGT]GG BstAPI 200 GCAGAGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstNI 672 CCTGG CC^[AT]GG Cfr10I 43 GCCGGC [GA]^CCGG[CT] EagI 96 CGGCCG C^GGCCG EarI 465 GAAGAG CTCTTC(1/4) EclXI 96 CGGCCG C^GGCCG Eco52I 96 CGGCCG C^GGCCG EcoRI 474 GAATTC G^AATTC EcoRII 672 CCTGG ^CC[AT]GG FauI 108 GCGGG CCCGC(4/6) HaeII 419 GGCGCC [GA]GCGC^[CT] HapII 44 CCGG C^CGG HincII 316 GTTGAC GT[CT]^[GA]AC HindII 316 GTTGAC GT[CT]^[GA]AC HpaII 44 CCGG C^CGG KasI 419 GGCGCC G^GCGCC Ksp632I 465 GAAGAG CTCTTC(1/4) MaeI 875 CTAG C^TAG MseI 254 TTAA T^TAA MspI 44 CCGG C^CGG MvaI 672 CCTGG CC^[AT]GG NaeI 43 GCCGGC GCC^GGC NarI 419 GGCGCC GG^CGCC NgoMIV 43 GCCGGC G^CCGGC NspI 175 GCATGT [GA]CATG^[CT] ScrFI 672 CCTGG CC^[ACGT]GG SfoI 419 GGCGCC GGC^GCC StuI 80 AGGCCT AGG^CCT TfiI 676 GAATC G^A[AT]TC Tru9I 254 TTAA T^TAA XcmI 386 CCAGAGCTCTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AcsI 10 AAATTC [GA]^AATT[CT] 474 GAATTC AcyI 354 GACGTC G[GA]^CG[CT]C 419 GGCGCC AlwNI 17 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG 201 CAGAGACTG ApoI 10 AAATTC [GA]^AATT[CT] 474 GAATTC Asp700I 148 GAAAGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 783 GAAGGCATTC AvaII 714 GGTCC G^G[AT]CC 883 GGTCC BanI 419 GGCGCC G^G[CT][GA]CC 447 GGTGCC BsaHI 354 GACGTC G[GA]^CG[CT]C 419 GGCGCC BsgI 747 CTGCAC GTGCAG(16/14) 887 CTGCAC BsrDI 47 GCAATG GCAATG(2/0) 599 GCAATG BsrI 231 ACTGG ACTGG(1/-1) 69 CCAGT DdeI 413 CTGAG C^T[ACGT]AG 461 CTCAG EaeI 66 CGGCCA [CT]^GGCC[GA] 96 CGGCCG Hin1I 354 GACGTC G[GA]^CG[CT]C 419 GGCGCC NlaIII 176 CATG CATG^ 396 CATG PleI 19 GACTC GAGTC(4/5) 400 GACTC SacI 31 GAGCTC GAGCT^C 389 GAGCTC Tsp45I 179 GTCAC ^GT[GC]AC 352 GTGAC Tsp509I 11 AATT ^AATT 475 AATT XmnI 148 GAAAGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 783 GAAGGCATTC ######################################### # 3 OR MORE CUT ENZYMES ( 42 enzymes) # ######################################### AccII AciI AfaI AluI AspHI BanII BbvI BmyI BsaJI BsiHKAI BsiYI BslI Bsp1286I BstF5I BstUI Cac8I CfoI Cfr13I EcoT14I Fnu4HI FokI HaeIII HhaI HinP1I HinfI ItaI MaeII MaeIII MboII MnlI MvnI MwoI NlaIV PstI RsaI Sau96I SfaNI SfcI SmlI StyI TseI TspRI