Restriction map for
pFN21AE1568
(Circular) (Six-base) MAPSORT of: pFN21AE1568.seq Check: 7720 from: 1 to: 5632
pFN21AE1568 5632 bp
With 182 enzymes: SgfI *
August 16, 2010 14:46 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2363 3442 278
Size: 53 83 186 936 827 1079 2468
Fragments arranged by size:
2468 1079 936 827 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2975 1937
Size: 1038 4594
Acc65I G'GTAC_C
Cuts at: 2953 2953
Size: 5632
AclI AA'CG_TT
Cuts at: 1563 3759 4132 1563
Size: 2196 373 3063
Fragments arranged by size:
3063 2196 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2121 2214 2236 2450 2612 2744 3695 5079
Size: 489 93 22 214 162 132 951 1384
Cuts at: 5079 5347 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 951 489 268 214 162 132
93 22
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5435
AflIII A'CryG_T
Cuts at: 1203 2929 4531 5508 1203
Size: 1726 1602 977 1327
Fragments arranged by size:
1726 1602 1327 977
AgeI A'CCGG_T
Cuts at: 4463 4463
Size: 5632
AhdI GACnn_n'nnGTC
Cuts at: 4361 4361
Size: 5632
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5632
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5632
AlwNI CAG_nnn'CTG
Cuts at: 2027 2211 3375 4947 2027
Size: 184 1164 1572 2712
Fragments arranged by size:
2712 1572 1164 184
ApaLI G'TGCA_C
Cuts at: 3689 4845 3689
Size: 1156 4476
ApoI r'AATT_y
Cuts at: 1493 2015 2479 2941 3167 3319 5428 1493
Size: 522 464 462 226 152 2109 1697
Fragments arranged by size:
2109 1697 522 464 462 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4186 160
Size: 872 3154 1606
Fragments arranged by size:
3154 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5632
AvaI C'yCGr_G
Cuts at: 1870 1943 2957 1870
Size: 73 1014 4545
Fragments arranged by size:
4545 1014 73
AvrII C'CTAG_G
Cuts at: 2879 2879
Size: 5632
BamHI G'GATC_C
Cuts at: 2962 3329 2962
Size: 367 5265
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2424 2452 2953 4408 618
Size: 325 217 627 637 28 501 1455 1842
Fragments arranged by size:
1842 1455 637 627 501 325 217 28
BanII G_rGCy'C
Cuts at: 1391 2040 2398 2809 2951 1391
Size: 649 358 411 142 4072
Fragments arranged by size:
4072 649 411 358 142
BbeI G_GCGC'C
Cuts at: 2428 2428
Size: 5632
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4661
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3823 3857 1903
Size: 34 1886 34 3678
Fragments arranged by size:
3678 1886 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3524 4734 3524
Size: 1210 4422
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5632
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2422 4243 136
Size: 107 122 71 631 605 750 1821 1525
Fragments arranged by size:
1821 1525 750 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5627 5627
Size: 5632
BlpI GC'TnA_GC
Cuts at: 3049 3049
Size: 5632
Bme1580I G_kGCm'C
Cuts at: 1790 3693 4849 1790
Size: 1903 1156 2573
Fragments arranged by size:
2573 1903 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5632
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3363 4321 442
Size: 416 53 83 399 24 1946 958 1753
Fragments arranged by size:
1946 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5632
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2318 4292 1239
Size: 1079 1974 2579
Fragments arranged by size:
2579 1974 1079
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2750 2793 2810 3757 4622 4920 2750
Size: 43 17 947 865 298 3462
Fragments arranged by size:
3462 947 865 298 43 17
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4295 882
Size: 617 2796 2219
Fragments arranged by size:
2796 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2691 3423 493
Size: 711 1487 732 2702
Fragments arranged by size:
2702 1487 732 711
BsaBI GATnn'nnATC
Cuts at: 5626 5626
Size: 5632
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2360 2425 3439 3821
Size: 53 83 186 936 827 65 1014 382
Cuts at: 3821 4456 275
Size: 635 1451
Fragments arranged by size:
1451 1014 936 827 635 382 186 83
65 53
BsaWI w'CCGG_w
Cuts at: 4065 4463 4737 4884 5295 4065
Size: 398 274 147 411 4402
Fragments arranged by size:
4402 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5407
Fragments arranged by size:
5407 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2048 1186
Size: 862 4770
BseYI C'CCAG_C
Cuts at: 2564 4835 5331 2564
Size: 2271 496 2865
Fragments arranged by size:
2865 2271 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2737 2877 1163
Size: 573 1001 140 3918
Fragments arranged by size:
3918 1001 573 140
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2104 3082 3843 4871 1540
Size: 275 186 103 978 761 1028 2301
Fragments arranged by size:
2301 1028 978 761 275 186 103
BsiHKAI G_wGCw'C
Cuts at: 2040 2398 2707 3693 3778 4849 2040
Size: 358 309 986 85 1071 2823
Fragments arranged by size:
2823 1071 986 358 309 85
BsmI GAATG_Cn'
Cuts at: 2792 3143 3236 2792
Size: 351 93 5188
Fragments arranged by size:
5188 351 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2040 2398 2707 2809 2951 3693 3778
Size: 399 250 358 309 102 142 742 85
Cuts at: 3778 4849 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 358 309 250 142
102 85
BspEI T'CCGG_A
Cuts at: 5295 5295
Size: 5632
BspHI T'CATG_A
Cuts at: 3521 5251 3521
Size: 1730 3902
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2987 844
Size: 2143 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3519 1394
Size: 429 1696 3507
Fragments arranged by size:
3507 1696 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2059 2611 4127 4301 65
Size: 1183 811 552 1516 174 1396
Fragments arranged by size:
1516 1396 1183 811 552 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2048 4276 4463 1700
Size: 110 238 2228 187 2869
Fragments arranged by size:
2869 2228 238 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5632
BssHII G'CGCG_C
Cuts at: 1925 2106 1925
Size: 181 5451
BssSI C'ACGA_G
Cuts at: 3692 4704 3692
Size: 1012 4620
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2211 794
Size: 1417 4215
BstBI TT'CG_AA
Cuts at: 4436 4436
Size: 5632
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5632
BstYI r'GATC_y
Cuts at: 1972 2962 3329 3714 3731 5183 5269 5281 5627
Size: 990 367 385 17 1452 86 12 346
Cuts at: 5627 1972
Size: 1977
Fragments arranged by size:
1977 1452 990 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4141
Fragments arranged by size:
4141 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4893
Fragments arranged by size:
4893 683 56
BtsI GCAGTG_nn'
Cuts at: 2748 3148 3942 3962 2748
Size: 400 794 20 4418
Fragments arranged by size:
4418 794 400 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5632
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5597
DraI TTT'AAA
Cuts at: 2936 2936
Size: 5632
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4639 809
Size: 507 3323 1802
Fragments arranged by size:
3323 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2071 2101 3079
Size: 54 1425 50 275 18 241 30 978
Cuts at: 3079 3968 5597 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 978 889 275 241 54 50
43 30 18
EagI C'GGCC_G
Cuts at: 1537 1812 2101 3079 1537
Size: 275 289 978 4090
Fragments arranged by size:
4090 978 289 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2465 3562 1353
Size: 1112 1097 3423
Fragments arranged by size:
3423 1112 1097
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4219 4593 4739 387
Size: 1064 138 2630 374 146 1280
Fragments arranged by size:
2630 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2038 2396 2038
Size: 358 5274
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2121 2214 2236 2318 2450 2612 2744
Size: 393 489 93 22 82 132 162 132
Cuts at: 2744 3695 4292 5079 5347 1239
Size: 951 597 787 268 1524
Fragments arranged by size:
1524 951 787 597 489 393 268 162
132 132 93 82 22
EcoRI G'AATT_C
Cuts at: 2479 2941 3319 2479
Size: 462 378 4792
Fragments arranged by size:
4792 462 378
FspI TGC'GCA
Cuts at: 4138 4138
Size: 5632
HaeII r_GCGC'y
Cuts at: 1678 2428 4779 1678
Size: 750 2351 2531
Fragments arranged by size:
2531 2351 750
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2199 2231 2380 2412 2822
Size: 32 135 32 627 32 149 32 410
Cuts at: 2822 2854 4273 4305 4347 4379 1373
Size: 32 1419 32 42 32 2626
Fragments arranged by size:
2626 1419 627 410 149 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2323 2976 3222 669
Size: 988 281 385 653 246 3079
Fragments arranged by size:
3079 988 653 385 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5632
HpaI GTT'AAC
Cuts at: 3222 3222
Size: 5632
KasI G'GCGC_C
Cuts at: 2424 2424
Size: 5632
KpnI G_GTAC'C
Cuts at: 2957 2957
Size: 5632
MfeI C'AATT_G
Cuts at: 3231 3231
Size: 5632
MluI A'CGCG_T
Cuts at: 5508 5508
Size: 5632
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2193 4746 4930 1305
Size: 888 2553 184 2007
Fragments arranged by size:
2553 2007 888 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3810
Fragments arranged by size:
3810 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3590 3949 4108 518
Size: 648 2424 359 159 2042
Fragments arranged by size:
2424 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3048 3725 4873 5118 1606
Size: 1442 677 1148 245 2120
Fragments arranged by size:
2120 1442 1148 677 245
NaeI GCC'GGC
Cuts at: 2050 2050
Size: 5632
NarI GG'CG_CC
Cuts at: 2425 2425
Size: 5632
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4141
Fragments arranged by size:
4141 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5632
NgoMIV G'CCGG_C
Cuts at: 2048 2048
Size: 5632
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5632
NotI GC'GGCC_GC
Cuts at: 3079 3079
Size: 5632
NspI r_CATG'y
Cuts at: 1132 1229 2184 2990 4535 1132
Size: 97 955 806 1545 2229
Fragments arranged by size:
2229 1545 955 806 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5632
PciI A'CATG_T
Cuts at: 4531 4531
Size: 5632
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5632
PmeI GTTT'AAAC
Cuts at: 2936 2936
Size: 5632
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3701 3733 5214 5246 3701
Size: 32 1481 32 4087
Fragments arranged by size:
4087 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5632
PsiI TTA'TAA
Cuts at: 3202 3202
Size: 5632
PstI C_TGCA'G
Cuts at: 830 2124 2207 2343 2984 830
Size: 1294 83 136 641 3478
Fragments arranged by size:
3478 1294 641 136 83
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5632
SacI G_AGCT'C
Cuts at: 2040 2398 2040
Size: 358 5274
SalI G'TCGA_C
Cuts at: 1936 2974 1936
Size: 1038 4594
SbfI CC_TGCA'GG
Cuts at: 2984 2984
Size: 5632
ScaI AGT'ACT
Cuts at: 3880 5592 3880
Size: 1712 3920
SfcI C'TryA_G
Cuts at: 826 1045 2120 2203 2339 2797 2867 2980 4115
Size: 219 1075 83 136 458 70 113 1135
Cuts at: 4115 4796 4987 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1075 681 458 219 191 136
113 83 70
SfoI GGC'GCC
Cuts at: 2426 2426
Size: 5632
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5632
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5632
SmaI CCC'GGG
Cuts at: 1872 2959 1872
Size: 1087 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2765 2808 2825 3736 4637 4899
Size: 197 926 822 43 17 911 901 262
Cuts at: 4899 820
Size: 1553
Fragments arranged by size:
1553 926 911 901 822 262 197 43
17
SnaBI TAC'GTA
Cuts at: 493 3423 493
Size: 2930 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5632
SphI G_CATG'C
Cuts at: 1132 2990 1132
Size: 1858 3774
SspI AAT'ATT
Cuts at: 5 52 3556 5
Size: 47 3504 2081
Fragments arranged by size:
3504 2081 47
StuI AGG'CCT
Cuts at: 1517 2087 1517
Size: 570 5062
StyI C'CwwG_G
Cuts at: 513 1065 2004 2241 2448 2879 3071 513
Size: 552 939 237 207 431 192 3074
Fragments arranged by size:
3074 939 552 431 237 207 192
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3659 3676 3829 4014 4459 325
Size: 1255 2079 17 153 185 445 1498
Fragments arranged by size:
2079 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2649 2730 3878
Size: 274 80 33 51 1443 672 81 1148
Cuts at: 3878 5590 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1148 672 274 138 81 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2769 2801 5592 5624 2769
Size: 32 2791 32 2777
Fragments arranged by size:
2791 2777 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5632
XbaI T'CTAG_A
Cuts at: 2968 2968
Size: 5632
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2398 2398
Size: 5632
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5632
XmaI C'CCGG_G
Cuts at: 1870 2957 1870
Size: 1087 4545
XmnI GAAnn'nnTTC
Cuts at: 2157 2792 3761 2157
Size: 635 969 4028
Fragments arranged by size:
4028 969 635
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2361 3440 276
Size: 53 83 186 936 827 1079 2468
Fragments arranged by size:
2468 1079 936 827 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI SacI SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoNI EcoO109I EcoRV FalI FseI FspAI NruI NsiI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII
RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI