Restriction map for
pFN21AB6103
##################################
# NO CUT ENZYMES ( 142 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BalI BanI BbeI BbrPI
BbvCI BciVI BclI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaWI BseAI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstEII BstPI BstUI
BstXI Bsu36I CelII Cfr10I ClaI
CpoI DraII DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HgaI Hin1I HphI KasI KpnI
KspI MamI MfeI MluI MluNI
MroI MscI MunI MvnI NaeI
NarI NcoI NgoMIV NheI NotI
NruI NsiI NspI PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PvuI RcaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfoI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI Tth111I XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
AccI 1103 GTATAC GT^[AC][GT]AC
AciI 1098 CCGC CCGC(-3/-1)
AclI 400 AACGTT AA^CGTT
AfaI 1091 GTAC GT^AC
AlwNI 656 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 1598 GGGCCC GGGCC^C
Asp700I 970 GAAAGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 1620 GGACC G^G[AT]CC
BamHI 1408 GGATCC G^GATCC
BanII 1598 GGGCCC G[GA]GC[CT]^C
BbsI 22 GAAGAC GAAGAC(2/6)
BcgI 543 CGAATATTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 1596 CCGGG CC^[GC]GG
BmyI 1598 GGGCCC G[AGT]GC[ACT]^C
BpmI 34 CTGGAG CTGGAG(16/14)
BpuAI 22 GAAGAC GAAGAC(2/6)
BsaI 521 GGTCTC GGTCTC(1/5)
BsiYI 1176 CCACAATTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 1176 CCACAATTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 522 GTCTC GTCTC(1/5)
BsmFI 1619 GGGAC GGGAC(10/14)
BsmI 1567 GCATTC GAATGC(1/-1)
Bsp1286I 1598 GGGCCC G[AGT]GC[ACT]^C
Bst1107I 1103 GTATAC GTA^TAC
BstAPI 807 GCAAACTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 977 TTCGAA TT^CGAA
BstYI 1408 GGATCC [GA]^GATC[CT]
BstZ17I 1103 GTATAC GTA^TAC
CfoI 118 GCGC GCG^C
DraI 1138 TTTAAA TTT^AAA
DraIII 43 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 181 GACCAGGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 1056 GAATTC G^AATTC
HapII 1596 CCGG C^CGG
HhaI 118 GCGC GCG^C
HinP1I 118 GCGC G^CGC
HindIII 1627 AAGCTT A^AGCTT
HpaI 1605 GTTAAC GTT^AAC
HpaII 1596 CCGG C^CGG
MflI 1408 GGATCC [GA]^GATC[CT]
MslI 1276 CACCTCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1495 CAGCTG C[AC]G^C[GT]G
MspI 1596 CCGG C^CGG
NciI 1596 CCGGG CC^[GC]GG
NdeI 1084 CATATG CA^TATG
NspV 977 TTCGAA TT^CGAA
PflMI 1176 CCACAATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1314 GAGTC GAGTC(4/5)
Psp1406I 400 AACGTT AA^CGTT
PstI 775 CTGCAG CTGCA^G
PvuII 1495 CAGCTG CAG^CTG
RsaI 1091 GTAC GT^AC
SapI 1209 GAAGAGC GCTCTTC(1/4)
SfuI 977 TTCGAA TT^CGAA
Tsp45I 710 GTGAC ^GT[GC]AC
Van91I 1176 CCACAATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 78 TCTAGA T^CTAGA
XcmI 1215 CCAGCAAATCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1408 GGATCC [GA]^GATC[CT]
XmnI 970 GAAAGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AseI 507 ATTAAT AT^TAAT
1671 ATTAAT
AsnI 507 ATTAAT AT^TAAT
1671 ATTAAT
BsaJI 517 CCAAGG C^C[ACGT][ACGT]GG
918 CCTTGG
Cac8I 114 GCGAGC GC[ACGT]^[ACGT]GC
1214 GCCAGC
EcoT14I 517 CCAAGG C^C[AT][AT]GG
918 CCTTGG
MaeII 401 ACGT A^CGT
1007 ACGT
MaeIII 710 GTGAC ^GT[ACGT]AC
818 GTAAC
PshBI 507 ATTAAT AT^TAAT
1671 ATTAAT
SfcI 260 CTACAG C^T[GA][CT]AG
775 CTGCAG
SspI 545 AATATT AAT^ATT
725 AATATT
StyI 517 CCAAGG C^C[AT][AT]GG
918 CCTTGG
TaqI 978 TCGA T^CGA
1079 TCGA
TthHB8I 978 TCGA T^CGA
1079 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AcsI AluI AlwI ApoI BbvI
BfaI BseRI BsrI BssKI BstF5I
BstNI Cfr13I DdeI DpnII EarI
EcoRII Fnu4HI FokI HaeIII HincII
HindII HinfI ItaI Ksp632I MaeI
MboI MboII MnlI MseI MvaI
MwoI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI SfaNI TfiI Tru9I
TseI Tsp509I TspRI