(Circular) (Six-base) MAPSORT of: pFN21AB6103.seq Check: 9542 from: 1 to: 6436 pFN21AB6103 6436 bp With 182 enzymes: SgfI * April 15, 2010 09:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4246 278 Size: 53 83 186 936 2710 2468 Fragments arranged by size: 2710 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3109 3779 1937 Size: 1172 670 4594 Fragments arranged by size: 4594 1172 670 Acc65I G'GTAC_C Cuts at: 3757 3757 Size: 6436 AclI AA'CG_TT Cuts at: 1563 2406 4563 4936 1563 Size: 843 2157 373 3063 Fragments arranged by size: 3063 2157 843 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2319 2421 3390 4499 5883 6151 1632 Size: 687 102 969 1109 1384 268 1917 Fragments arranged by size: 1917 1384 1109 969 687 268 102 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6239 AflIII A'CryG_T Cuts at: 1203 5335 6312 1203 Size: 4132 977 1327 Fragments arranged by size: 4132 1327 977 AgeI A'CCGG_T Cuts at: 5267 5267 Size: 6436 AhdI GACnn_n'nnGTC Cuts at: 5165 5165 Size: 6436 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6436 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6436 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2220 2252 2220 Size: 32 6404 AlwNI CAG_nnn'CTG Cuts at: 2666 4179 5751 2666 Size: 1513 1572 3351 Fragments arranged by size: 3351 1572 1513 ApaI G_GGCC'C Cuts at: 3607 3607 Size: 6436 ApaLI G'TGCA_C Cuts at: 4493 5649 4493 Size: 1156 5280 ApoI r'AATT_y Cuts at: 1493 2474 3061 3140 3474 3617 3745 3971 4123 Size: 981 587 79 334 143 128 226 152 Cuts at: 4123 6232 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 981 587 334 226 152 143 128 79 AseI AT'TA_AT Cuts at: 160 1032 2513 3677 4990 160 Size: 872 1481 1164 1313 1606 Fragments arranged by size: 1606 1481 1313 1164 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6436 AvaI C'yCGr_G Cuts at: 1870 1943 3761 1870 Size: 73 1818 4545 Fragments arranged by size: 4545 1818 73 BamHI G'GATC_C Cuts at: 3413 3766 4133 3413 Size: 353 367 5716 Fragments arranged by size: 5716 367 353 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3757 5212 618 Size: 325 217 627 1970 1455 1842 Fragments arranged by size: 1970 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3607 3755 1391 Size: 2216 148 4072 Fragments arranged by size: 4072 2216 148 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2034 928 Size: 971 135 5330 Fragments arranged by size: 5330 971 135 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2537 2571 4627 4661 1903 Size: 34 600 34 2056 34 3678 Fragments arranged by size: 3678 2056 600 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4328 5538 4328 Size: 1210 5226 BclI T'GATC_A Cuts at: 1548 1548 Size: 6436 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5047 136 Size: 107 122 71 631 605 3375 1525 Fragments arranged by size: 3375 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6431 6431 Size: 6436 BlpI GC'TnA_GC Cuts at: 3853 3853 Size: 6436 Bme1580I G_kGCm'C Cuts at: 1790 3607 4497 5653 1790 Size: 1817 890 1156 2573 Fragments arranged by size: 2573 1817 1156 890 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6436 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3601 4167 5125 Size: 416 53 83 399 24 2184 566 958 Cuts at: 5125 442 Size: 1753 Fragments arranged by size: 2184 1753 958 566 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6436 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2060 5096 1239 Size: 821 3036 2579 Fragments arranged by size: 3036 2579 821 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4561 5426 5724 4561 Size: 865 298 5273 Fragments arranged by size: 5273 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2532 5099 882 Size: 617 1033 2567 2219 Fragments arranged by size: 2567 2219 1033 617 BsaAI yAC'GTr Cuts at: 493 1204 4227 493 Size: 711 3023 2702 Fragments arranged by size: 3023 2702 711 BsaBI GATnn'nnATC Cuts at: 6430 6430 Size: 6436 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4243 4625 5260 275 Size: 53 83 186 936 2710 382 635 1451 Fragments arranged by size: 2710 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4869 5267 5541 5688 6099 4869 Size: 398 274 147 411 5206 Fragments arranged by size: 5206 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6211 Fragments arranged by size: 6211 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2112 3222 3282 3743 1186 Size: 926 1110 60 461 3879 Fragments arranged by size: 3879 1110 926 461 60 BseYI C'CCAG_C Cuts at: 5639 6135 5639 Size: 496 5940 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5863 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3886 4647 5675 1540 Size: 275 186 1885 761 1028 2301 Fragments arranged by size: 2301 1885 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4497 4582 5653 4497 Size: 85 1071 5280 Fragments arranged by size: 5280 1071 85 BsmI GAATG_Cn' Cuts at: 3572 3947 4040 3572 Size: 375 93 5968 Fragments arranged by size: 5968 375 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3607 3755 4497 4582 5653 1391 Size: 399 1817 148 742 85 1071 2174 Fragments arranged by size: 2174 1817 1071 742 399 148 85 BspEI T'CCGG_A Cuts at: 6099 6099 Size: 6436 BspHI T'CATG_A Cuts at: 4325 6055 4325 Size: 1730 4706 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3791 844 Size: 2947 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4323 1394 Size: 429 2500 3507 Fragments arranged by size: 3507 2500 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4931 5105 65 Size: 1183 3683 174 1396 Fragments arranged by size: 3683 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5080 5267 1700 Size: 110 3270 187 2869 Fragments arranged by size: 3270 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6436 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6436 BssSI C'ACGA_G Cuts at: 4496 5508 4496 Size: 1012 5424 BstAPI GCAn_nnn'nTGC Cuts at: 794 2818 794 Size: 2024 4412 BstBI TT'CG_AA Cuts at: 2983 5240 2983 Size: 2257 4179 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6436 BstYI r'GATC_y Cuts at: 1972 3413 3766 4133 4518 4535 5987 6073 6085 Size: 1441 353 367 385 17 1452 86 12 Cuts at: 6085 6431 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1441 385 367 353 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3110 3110 Size: 6436 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5884 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5697 Fragments arranged by size: 5697 683 56 BtsI GCAGTG_nn' Cuts at: 2777 3566 3952 4746 4766 2777 Size: 789 386 794 20 4447 Fragments arranged by size: 4447 794 789 386 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6436 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6401 DraI TTT'AAA Cuts at: 3145 3740 3145 Size: 595 5841 DraIII CAC_nnn'GTG Cuts at: 2053 2053 Size: 6436 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2192 5443 809 Size: 507 876 3251 1802 Fragments arranged by size: 3251 1802 876 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3883 4772 6401 Size: 54 1425 50 275 18 2053 889 1629 Cuts at: 6401 8 Size: 43 Fragments arranged by size: 2053 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3883 1537 Size: 275 2071 4090 Fragments arranged by size: 4090 2071 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2224 2595 3173 3209 3399 3720 4366 1353 Size: 871 371 578 36 190 321 646 3423 Fragments arranged by size: 3423 871 646 578 371 321 190 36 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5023 5397 5543 387 Size: 1064 138 3434 374 146 1280 Fragments arranged by size: 3434 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2060 2319 2421 3390 4499 5096 5883 Size: 393 428 259 102 969 1109 597 787 Cuts at: 5883 6151 1239 Size: 268 1524 Fragments arranged by size: 1524 1109 969 787 597 428 393 268 259 102 EcoRI G'AATT_C Cuts at: 3061 3745 4123 3061 Size: 684 378 5374 Fragments arranged by size: 5374 684 378 FspI TGC'GCA Cuts at: 4942 4942 Size: 6436 HaeII r_GCGC'y Cuts at: 1678 5583 1678 Size: 3905 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2626 2658 5077 5109 5151 Size: 32 135 32 1054 32 2419 32 42 Cuts at: 5151 5183 1373 Size: 32 2626 Fragments arranged by size: 2626 2419 1054 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2137 2484 3612 3780 4026 669 Size: 988 281 199 347 1128 168 246 3079 Fragments arranged by size: 3079 1128 988 347 281 246 199 168 HindIII A'AGCT_T Cuts at: 748 3632 748 Size: 2884 3552 HpaI GTT'AAC Cuts at: 3612 4026 3612 Size: 414 6022 KpnI G_GTAC'C Cuts at: 3761 3761 Size: 6436 MfeI C'AATT_G Cuts at: 4035 4035 Size: 6436 MluI A'CGCG_T Cuts at: 6312 6312 Size: 6436 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2586 5550 5734 1305 Size: 1281 2964 184 2007 Fragments arranged by size: 2964 2007 1281 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4614 Fragments arranged by size: 4614 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3285 4394 4753 4912 518 Size: 648 2119 1109 359 159 2042 Fragments arranged by size: 2119 2042 1109 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3502 3852 4529 5677 5922 1606 Size: 1896 350 677 1148 245 2120 Fragments arranged by size: 2120 1896 1148 677 350 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5884 NdeI CA'TA_TG Cuts at: 387 3090 387 Size: 2703 3733 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6436 NotI GC'GGCC_GC Cuts at: 3883 3883 Size: 6436 NspI r_CATG'y Cuts at: 1132 1229 3794 5339 1132 Size: 97 2565 1545 2229 Fragments arranged by size: 2565 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6436 PciI A'CATG_T Cuts at: 5335 5335 Size: 6436 PflMI CCAn_nnn'nTGG Cuts at: 1108 3187 1108 Size: 2079 4357 PfoI T'CCnGG_A Cuts at: 3034 3034 Size: 6436 PmeI GTTT'AAAC Cuts at: 3740 3740 Size: 6436 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4505 4537 6018 6050 4505 Size: 32 1481 32 4891 Fragments arranged by size: 4891 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6436 PsiI TTA'TAA Cuts at: 3638 4006 3638 Size: 368 6068 PspOMI G'GGCC_C Cuts at: 3603 3603 Size: 6436 PstI C_TGCA'G Cuts at: 830 2784 3788 830 Size: 1954 1004 3478 Fragments arranged by size: 3478 1954 1004 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6436 PvuII CAG'CTG Cuts at: 3502 3502 Size: 6436 SalI G'TCGA_C Cuts at: 1936 3778 1936 Size: 1842 4594 SapI GCTCTTCn'nnn_ Cuts at: 3209 3209 Size: 6436 SbfI CC_TGCA'GG Cuts at: 3788 3788 Size: 6436 ScaI AGT'ACT Cuts at: 4684 6396 4684 Size: 1712 4724 SfcI C'TryA_G Cuts at: 826 1045 2265 2780 3784 4919 5600 5791 826 Size: 219 1220 515 1004 1135 681 191 1471 Fragments arranged by size: 1471 1220 1135 1004 681 515 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6436 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6436 SmaI CCC'GGG Cuts at: 1872 3763 1872 Size: 1891 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4540 5441 5703 820 Size: 197 926 2597 901 262 1553 Fragments arranged by size: 2597 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4227 493 Size: 3734 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6436 SphI G_CATG'C Cuts at: 1132 3794 1132 Size: 2662 3774 SspI AAT'ATT Cuts at: 5 52 2552 2732 4360 5 Size: 47 2500 180 1628 2081 Fragments arranged by size: 2500 2081 1628 180 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6436 StyI C'CwwG_G Cuts at: 513 1065 2522 2923 3875 513 Size: 552 1457 401 952 3074 Fragments arranged by size: 3074 1457 952 552 401 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4463 4480 4633 4818 5263 325 Size: 1255 2883 17 153 185 445 1498 Fragments arranged by size: 2883 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4682 6394 96 Size: 274 80 33 51 1443 2705 1712 138 Fragments arranged by size: 2705 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3030 3062 6396 6428 3030 Size: 32 3334 32 3038 Fragments arranged by size: 3334 3038 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6436 XbaI T'CTAG_A Cuts at: 2083 3772 2083 Size: 1689 4747 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3227 3227 Size: 6436 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6436 XmaI C'CCGG_G Cuts at: 1870 3761 1870 Size: 1891 4545 XmnI GAAnn'nnTTC Cuts at: 2979 4565 2979 Size: 1586 4850 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4244 276 Size: 53 83 186 936 2710 2468 Fragments arranged by size: 2710 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI Bsu36I EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI