################################## # NO CUT ENZYMES ( 155 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AgeI AhdI AlwNI AocI Aor51HI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BbsI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaI BsaWI BseAI BseRI BsiEI BsiWI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrFI BsrGI BssHII Bst1107I BstBI BstEII BstF5I BstPI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV EcoT14I FbaI FokI FseI FspI HapII HgaI HincII HindII HindIII HpaI HpaII KpnI MaeI MaeIII MfeI MluNI MroI MscI MslI MspI MunI NaeI NciI NcoI NdeI NgoMIV NheI NlaIII NotI NruI NspI NspV PacI PaeR7I PflFI PinAI PleI PmeI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SalI SbfI ScaI SexAI SfcI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI Tsp45I Tth111I TthHB8I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AcyI 3 GGCGCC G[GA]^CG[CT]C AfaI 143 GTAC GT^AC AflIII 514 ACGCGT A^C[GA][CT]GT Alw44I 155 GTGCAC G^TGCAC ApaI 257 GGGCCC GGGCC^C ApaLI 155 GTGCAC G^TGCAC AspHI 155 GTGCAC G[AT]GC[AT]^C BbeI 3 GGCGCC GGCGC^C BbrPI 342 CACGTG CAC^GTG BpmI 262 CTCCAG CTGGAG(16/14) BsaAI 342 CACGTG [CT]AC^GT[GA] BsaBI 721 GATCTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 3 GGCGCC G[GA]^CG[CT]C BsaJI 41 CCGCGG C^C[ACGT][ACGT]GG BsgI 491 CTGCAC GTGCAG(16/14) BsiHKAI 155 GTGCAC G[AT]GC[AT]^C BsiYI 341 CCACGTGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 341 CCACGTGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsrBI 303 GAGCGG CCGCTC(-3/-3) BssKI 730 CCTGG ^CC[ACGT]GG BssSI 543 CACGAG CACGAG(-5/-1) BstAPI 832 GCAGTTGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstNI 730 CCTGG CC^[AT]GG BstXI 191 CCAAATGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CfoI 4 GCGC GCG^C DsaI 41 CCGCGG C^C[GA][CT]GG EaeI 101 TGGCCG [CT]^GGCC[GA] EcoRI 772 GAATTC G^AATTC EcoRII 730 CCTGG ^CC[AT]GG EcoT22I 922 ATGCAT ATGCA^T HaeII 3 GGCGCC [GA]GCGC^[CT] HhaI 4 GCGC GCG^C Hin1I 3 GGCGCC G[GA]^CG[CT]C HinP1I 4 GCGC G^CGC HinfI 667 GAATC G^A[ACGT]TC KasI 3 GGCGCC G^GCGCC KspI 41 CCGCGG CCGC^GG MaeII 343 ACGT A^CGT MamI 721 GATCTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluI 514 ACGCGT A^CGCGT MspA1I 41 CCGCGG C[AC]G^C[GT]G MvaI 730 CCTGG CC^[AT]GG NarI 3 GGCGCC GG^CGCC NsiI 922 ATGCAT ATGCA^T PflMI 341 CCACGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 342 CACGTG CAC^GTG PmlI 342 CACGTG CAC^GTG PpuMI 14 AGGTCCT [GA]G^G[AT]CC[CT] RsaI 143 GTAC GT^AC SacII 41 CCGCGG CCGC^GG SapI 239 GAAGAGC GCTCTTC(1/4) ScrFI 730 CCTGG CC^[ACGT]GG SfaNI 921 GATGC GCATC(5/9) SfoI 3 GGCGCC GGC^GCC TfiI 667 GAATC G^A[AT]TC Van91I 341 CCACGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 264 CCAGAACAATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 10 enzymes) # ################################### AvaII 15 GGTCC G^G[AT]CC 693 GGACC BamHI 528 GGATCC G^GATCC 726 GGATCC BanI 3 GGCGCC G^G[CT][GA]CC 334 GGCACC BanII 257 GGGCCC G[GA]GC[CT]^C 307 GGGCTC BbvI 64 GCAGC GCAGC(8/12) 187 GCTGC BspMI 400 ACCTGC ACCTGC(4/8) 12 GCAGGT EarI 239 GAAGAG CTCTTC(1/4) 873 GAAGAG FauI 9 CCCGC CCCGC(4/6) 305 GCGGG Ksp632I 239 GAAGAG CTCTTC(1/4) 873 GAAGAG TseI 64 GCAGC G^C[AT]GC 187 GCTGC ######################################### # 3 OR MORE CUT ENZYMES ( 36 enzymes) # ######################################### AccII AciI AcsI AluI AlwI ApoI BmyI Bsp1286I BsrI BstUI BstYI Cac8I Cfr13I DdeI DpnII DraII EcoO109I Fnu4HI HaeIII HphI ItaI MboI MboII MflI MnlI MseI MvnI MwoI NdeII NlaIV Sau3AI Sau96I Tru9I Tsp509I TspRI XhoII