(Circular) (Six-base) MAPSORT of: pFN21AE6307.seq Check: 5833 from: 1 to: 5647 pFN21AE6307 5647 bp With 182 enzymes: SgfI * July 1, 2014 12:02 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3457 278 Size: 53 83 186 936 1921 2468 Fragments arranged by size: 2468 1921 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2990 1937 Size: 1053 4594 Acc65I G'GTAC_C Cuts at: 2968 2968 Size: 5647 AclI AA'CG_TT Cuts at: 1563 3774 4147 1563 Size: 2211 373 3063 Fragments arranged by size: 3063 2211 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2136 2277 2486 3710 5094 5362 1632 Size: 504 141 209 1224 1384 268 1917 Fragments arranged by size: 1917 1384 1224 504 268 209 141 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5450 AflIII A'CryG_T Cuts at: 1203 2519 4546 5523 1203 Size: 1316 2027 977 1327 Fragments arranged by size: 2027 1327 1316 977 AgeI A'CCGG_T Cuts at: 4478 4478 Size: 5647 AhdI GACnn_n'nnGTC Cuts at: 4376 4376 Size: 5647 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5647 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5647 AlwNI CAG_nnn'CTG Cuts at: 3390 4962 3390 Size: 1572 4075 ApaI G_GGCC'C Cuts at: 2266 2266 Size: 5647 ApaLI G'TGCA_C Cuts at: 2160 3704 4860 2160 Size: 1544 1156 2947 Fragments arranged by size: 2947 1544 1156 ApoI r'AATT_y Cuts at: 1493 2540 2596 2777 2956 3182 3334 5443 1493 Size: 1047 56 181 179 226 152 2109 1697 Fragments arranged by size: 2109 1697 1047 226 181 179 152 56 AseI AT'TA_AT Cuts at: 160 1032 4201 160 Size: 872 3169 1606 Fragments arranged by size: 3169 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5647 AvaI C'yCGr_G Cuts at: 1870 1943 2972 1870 Size: 73 1029 4545 Fragments arranged by size: 4545 1029 73 BamHI G'GATC_C Cuts at: 2533 2731 2977 3344 2533 Size: 198 246 367 4836 Fragments arranged by size: 4836 367 246 198 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2008 2339 2968 4423 618 Size: 325 217 627 221 331 629 1455 1842 Fragments arranged by size: 1842 1455 629 627 331 325 221 217 BanII G_rGCy'C Cuts at: 1391 2266 2316 2966 1391 Size: 875 50 650 4072 Fragments arranged by size: 4072 875 650 50 BbeI G_GCGC'C Cuts at: 2012 2012 Size: 5647 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4676 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3838 3872 1903 Size: 34 1901 34 3678 Fragments arranged by size: 3678 1901 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3539 4749 3539 Size: 1210 4437 BclI T'GATC_A Cuts at: 1548 1548 Size: 5647 BfrBI ATG'CAT Cuts at: 2929 2929 Size: 5647 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4258 136 Size: 107 122 71 631 605 2586 1525 Fragments arranged by size: 2586 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5642 5642 Size: 5647 BlpI GC'TnA_GC Cuts at: 3064 3064 Size: 5647 Bme1580I G_kGCm'C Cuts at: 1790 2164 2266 3708 4864 1790 Size: 374 102 1442 1156 2573 Fragments arranged by size: 2573 1442 1156 374 102 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5647 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3378 4336 442 Size: 416 53 83 399 24 1961 958 1753 Fragments arranged by size: 1961 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5647 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2252 4307 1239 Size: 1013 2055 2579 Fragments arranged by size: 2579 2055 1013 Bpu10I CC'TnA_GC Cuts at: 2073 2073 Size: 5647 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3772 4637 4935 3772 Size: 865 298 4484 Fragments arranged by size: 4484 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4310 882 Size: 617 2811 2219 Fragments arranged by size: 2811 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2349 3438 493 Size: 711 1145 1089 2702 Fragments arranged by size: 2702 1145 1089 711 BsaBI GATnn'nnATC Cuts at: 2730 5641 2730 Size: 2911 2736 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2009 3454 3836 4471 Size: 53 83 186 936 476 1445 382 635 Cuts at: 4471 275 Size: 1451 Fragments arranged by size: 1451 1445 936 635 476 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4080 4478 4752 4899 5310 4080 Size: 398 274 147 411 4417 Fragments arranged by size: 4417 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2327 2357 1178 Size: 30 165 30 924 30 4468 Fragments arranged by size: 4468 924 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5647 BseYI C'CCAG_C Cuts at: 2369 4850 5346 2369 Size: 2481 496 2670 Fragments arranged by size: 2670 2481 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2481 1163 Size: 573 745 4329 Fragments arranged by size: 4329 745 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3097 3858 4886 1540 Size: 275 186 1096 761 1028 2301 Fragments arranged by size: 2301 1096 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2164 3708 3793 4864 2164 Size: 1544 85 1071 2947 Fragments arranged by size: 2947 1544 1071 85 BsmI GAATG_Cn' Cuts at: 3158 3251 3158 Size: 93 5554 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2164 2266 2316 2966 3708 3793 4864 Size: 399 374 102 50 650 742 85 1071 Cuts at: 4864 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 650 399 374 102 85 50 BspEI T'CCGG_A Cuts at: 5310 5310 Size: 5647 BspHI T'CATG_A Cuts at: 3536 5266 3536 Size: 1730 3917 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2008 2414 3002 844 Size: 1164 406 588 3489 Fragments arranged by size: 3489 1164 588 406 BsrBI CCG'CTC Cuts at: 1394 1823 2310 3534 1394 Size: 429 487 1224 3507 Fragments arranged by size: 3507 1224 487 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4142 4316 65 Size: 1183 2894 174 1396 Fragments arranged by size: 2894 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4291 4478 1700 Size: 110 2481 187 2869 Fragments arranged by size: 2869 2481 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5647 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5647 BssSI C'ACGA_G Cuts at: 2548 3707 4719 2548 Size: 1159 1012 3476 Fragments arranged by size: 3476 1159 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2843 794 Size: 2049 3598 BstBI TT'CG_AA Cuts at: 4451 4451 Size: 5647 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5647 BstXI CCAn_nnnn'nTGG Cuts at: 2203 2203 Size: 5647 BstYI r'GATC_y Cuts at: 1972 2533 2725 2731 2977 3344 3729 3746 5198 Size: 561 192 6 246 367 385 17 1452 Cuts at: 5198 5284 5296 5642 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 561 385 367 346 246 192 86 17 12 6 BtgI C'CryG_G Cuts at: 513 1065 2004 2046 513 Size: 552 939 42 4114 Fragments arranged by size: 4114 939 552 42 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2316 487 Size: 683 56 1090 3818 Fragments arranged by size: 3818 1090 683 56 BtsI GCAGTG_nn' Cuts at: 2336 2364 3163 3957 3977 2336 Size: 28 799 794 20 4006 Fragments arranged by size: 4006 799 794 28 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5647 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5612 DraI TTT'AAA Cuts at: 2951 2951 Size: 5647 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4654 809 Size: 507 3338 1802 Fragments arranged by size: 3338 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2106 3094 3983 Size: 54 1425 50 275 18 276 988 889 Cuts at: 3983 5612 8 Size: 1629 43 Fragments arranged by size: 1629 1425 988 889 276 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3094 1537 Size: 275 1282 4090 Fragments arranged by size: 4090 1282 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2239 2873 3577 1353 Size: 886 634 704 3423 Fragments arranged by size: 3423 886 704 634 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4234 4608 4754 387 Size: 1064 138 2645 374 146 1280 Fragments arranged by size: 2645 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2136 2252 2277 2486 3710 4307 5094 Size: 393 504 116 25 209 1224 597 787 Cuts at: 5094 5362 1239 Size: 268 1524 Fragments arranged by size: 1524 1224 787 597 504 393 268 209 116 25 EcoO109I rG'GnC_Cy Cuts at: 2020 2262 2263 2020 Size: 242 1 5404 Fragments arranged by size: 5404 242 1 EcoRI G'AATT_C Cuts at: 2777 2956 3334 2777 Size: 179 378 5090 Fragments arranged by size: 5090 378 179 FspI TGC'GCA Cuts at: 4153 4153 Size: 5647 HaeII r_GCGC'y Cuts at: 1678 2012 4794 1678 Size: 334 2782 2531 Fragments arranged by size: 2782 2531 334 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4288 4320 4362 4394 1373 Size: 32 135 32 2716 32 42 32 2626 Fragments arranged by size: 2716 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2991 3237 669 Size: 988 281 1053 246 3079 Fragments arranged by size: 3079 1053 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5647 HpaI GTT'AAC Cuts at: 3237 3237 Size: 5647 KasI G'GCGC_C Cuts at: 2008 2008 Size: 5647 KpnI G_GTAC'C Cuts at: 2972 2972 Size: 5647 MfeI C'AATT_G Cuts at: 3246 3246 Size: 5647 MluI A'CGCG_T Cuts at: 2519 5523 2519 Size: 3004 2643 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4761 4945 1305 Size: 3456 184 2007 Fragments arranged by size: 3456 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3825 Fragments arranged by size: 3825 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3605 3964 4123 518 Size: 648 2439 359 159 2042 Fragments arranged by size: 2439 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2048 3063 3740 4888 5133 1606 Size: 442 1015 677 1148 245 2120 Fragments arranged by size: 2120 1148 1015 677 442 245 NarI GG'CG_CC Cuts at: 2009 2009 Size: 5647 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4156 Fragments arranged by size: 4156 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5647 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5647 NotI GC'GGCC_GC Cuts at: 3094 3094 Size: 5647 NsiI A_TGCA'T Cuts at: 2931 2931 Size: 5647 NspI r_CATG'y Cuts at: 1132 1229 3005 4550 1132 Size: 97 1776 1545 2229 Fragments arranged by size: 2229 1776 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5647 PciI A'CATG_T Cuts at: 4546 4546 Size: 5647 PflMI CCAn_nnn'nTGG Cuts at: 1108 2352 1108 Size: 1244 4403 PmeI GTTT'AAAC Cuts at: 2951 2951 Size: 5647 PmlI CAC'GTG Cuts at: 2349 2349 Size: 5647 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3716 3748 5229 5261 3716 Size: 32 1481 32 4102 Fragments arranged by size: 4102 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2020 2020 Size: 5647 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5647 PsiI TTA'TAA Cuts at: 3217 3217 Size: 5647 PspOMI G'GGCC_C Cuts at: 2262 2262 Size: 5647 PstI C_TGCA'G Cuts at: 830 2999 830 Size: 2169 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5647 SacII CC_GC'GG Cuts at: 2049 2049 Size: 5647 SalI G'TCGA_C Cuts at: 1936 2989 1936 Size: 1053 4594 SapI GCTCTTCn'nnn_ Cuts at: 2239 2239 Size: 5647 SbfI CC_TGCA'GG Cuts at: 2999 2999 Size: 5647 ScaI AGT'ACT Cuts at: 3895 5607 3895 Size: 1712 3935 SfcI C'TryA_G Cuts at: 826 1045 2995 4130 4811 5002 826 Size: 219 1950 1135 681 191 1471 Fragments arranged by size: 1950 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2010 2010 Size: 5647 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5647 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5647 SmaI CCC'GGG Cuts at: 1872 2974 1872 Size: 1102 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3751 4652 4914 820 Size: 197 926 1808 901 262 1553 Fragments arranged by size: 1808 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3438 493 Size: 2945 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5647 SphI G_CATG'C Cuts at: 1132 3005 1132 Size: 1873 3774 SspI AAT'ATT Cuts at: 5 52 3571 5 Size: 47 3519 2081 Fragments arranged by size: 3519 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5647 StyI C'CwwG_G Cuts at: 513 1065 2004 3086 513 Size: 552 939 1082 3074 Fragments arranged by size: 3074 1082 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2909 3674 3691 3844 4029 4474 325 Size: 1255 1329 765 17 153 185 445 1498 Fragments arranged by size: 1498 1329 1255 765 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2147 3893 5605 Size: 274 80 33 51 1443 170 1746 1712 Cuts at: 5605 96 Size: 138 Fragments arranged by size: 1746 1712 1443 274 170 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2841 2873 5607 5639 2841 Size: 32 2734 32 2849 Fragments arranged by size: 2849 2734 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5647 XbaI T'CTAG_A Cuts at: 2983 2983 Size: 5647 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2276 2276 Size: 5647 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5647 XmaI C'CCGG_G Cuts at: 1870 2972 1870 Size: 1102 4545 XmnI GAAnn'nnTTC Cuts at: 3776 3776 Size: 5647 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3455 276 Size: 53 83 186 936 1921 2468 Fragments arranged by size: 2468 1921 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI PacI PfoI PspXI PsrI PvuII RsrII SacI SanDI SexAI SfiI SrfI SwaI