################################## # NO CUT ENZYMES ( 104 enzymes) # ################################## Acc65I AccIII AflII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AviII AvrII BamHI BbrPI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BseAI BseRI BsiEI BsiHKAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspLU11I BsrGI BssSI BstBI BstEII BstPI CelII ClaI CpoI DraI DraIII EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRV EcoT22I FbaI FspI HindIII HpaI KpnI MfeI MluI MroI MunI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI PvuI RsrII SacI SalI SapI ScaI SexAI SfiI SfuI SgrAI SmlI SnaBI SpeI SphI SspBI SspI StuI SwaI XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AatII 430 GACGTC GACGT^C AclI 676 AACGTT AA^CGTT AflIII 380 ACACGT A^C[GA][CT]GT AlwI 721 GGATC GGATC(4/5) AocI 1507 CCTGAGG CC^T[ACGT]AGG BalI 233 TGGCCA TGG^CCA BbeI 217 GGCGCC GGCGC^C BbsI 478 GAAGAC GAAGAC(2/6) BbvCI 1129 CCTCAGC CCTCAGC(-5/-2) BpuAI 478 GAAGAC GAAGAC(2/6) BsaAI 381 CACGTA [CT]AC^GT[GA] BsaBI 285 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 634 GAGACC GGTCTC(1/5) BsaWI 1481 TCCGGT [AT]^CCGG[AT] BsgI 1298 GTGCAG GTGCAG(16/14) BspHI 1424 TCATGA T^CATGA BsrDI 528 CATTGC GCAATG(2/0) BssHII 54 GCGCGC G^CGCGC Bst1107I 792 GTATAC GTA^TAC BstYI 721 GGATCT [GA]^GATC[CT] BstZ17I 792 GTATAC GTA^TAC Bsu36I 1507 CCTGAGG CC^T[ACGT]AGG DrdI 1504 GACCCTGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 1507 CCTGAGG CC^T[ACGT]AGG FseI 1158 GGCCGGCC GGCCGG^CC KasI 217 GGCGCC G^GCGCC MamI 285 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 721 GGATCT [GA]^GATC[CT] MluNI 233 TGGCCA TGG^CCA MscI 233 TGGCCA TGG^CCA NarI 217 GGCGCC GG^CGCC NcoI 236 CCATGG C^CATGG PflMI 1397 CCATTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Psp1406I 676 AACGTT AA^CGTT PvuII 1021 CAGCTG CAG^CTG RcaI 1424 TCATGA T^CATGA SbfI 249 CCTGCAGG CCTGCA^GG SfoI 217 GGCGCC GGC^GCC Sse8387I 249 CCTGCAGG CCTGCA^GG Van91I 1397 CCATTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 1202 CCACAAATGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 721 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AccI 241 GTAGAC GT^[AC][GT]AC 792 GTATAC AspI 481 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC 1011 GACTGGGTC BfaI 404 CTAG C^TAG 595 CTAG BsmAI 634 GAGAC GTCTC(1/5) 782 GAGAC BsrBI 414 CCGCTC CCGCTC(-3/-3) 1376 CCGCTC BsrFI 703 GCCGGC [GA]^CCGG[CT] 1159 GCCGGC BstAPI 1300 GCAGAAATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1309 GCAGGTGCTGC BstXI 224 CCAACTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1226 CCACGGTGATGG Cfr10I 703 GCCGGC [GA]^CCGG[CT] 1159 GCCGGC EarI 808 GAAGAG CTCTTC(1/4) 1269 GAAGAG EcoRI 991 GAATTC G^AATTC 1038 GAATTC HincII 325 GTTGAC GT[CT]^[GA]AC 523 GTCAAC HindII 325 GTTGAC GT[CT]^[GA]AC 523 GTCAAC Ksp632I 808 GAAGAG CTCTTC(1/4) 1269 GAAGAG MaeI 404 CTAG C^TAG 595 CTAG MslI 687 CATGCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1227 CACGGTGATG NaeI 703 GCCGGC GCC^GGC 1159 GCCGGC NgoMIV 703 GCCGGC G^CCGGC 1159 GCCGGC PflFI 481 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC 1011 GACTGGGTC TaqI 644 TCGA T^CGA 1256 TCGA Tth111I 481 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC 1011 GACTGGGTC TthHB8I 644 TCGA T^CGA 1256 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 90 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwNI ApoI AvaI AvaII BanI BanII BbvI BcnI BmyI BpmI BsaHI BsaJI BsiYI BslI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KspI MaeII MaeIII MboI MboII MnlI MseI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI PstI RsaI SacII Sau3AI Sau96I ScrFI SfaNI SfcI SmaI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XmaCI XmaI