(Linear) (Six-base) MAPSORT of: KIBB5690.seq Check: 4728 from: 1 to: 1554 KIBB5690.orf With 182 enzymes: SgfI * July 15, 2010 18:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1300 1554 Size: 1300 254 AatII G_ACGT'C Cuts at: 0 434 1554 Size: 434 1120 AccI GT'mk_AC Cuts at: 0 242 793 1554 Size: 242 551 761 Fragments arranged by size: 761 551 242 AclI AA'CG_TT Cuts at: 0 677 1554 Size: 677 877 AflIII A'CryG_T Cuts at: 0 380 1554 Size: 380 1174 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 937 1554 Size: 937 617 AlwNI CAG_nnn'CTG Cuts at: 0 1315 1324 1367 1554 Size: 1315 9 43 187 Fragments arranged by size: 1315 187 43 9 ApoI r'AATT_y Cuts at: 0 552 830 991 1038 1328 1554 Size: 552 278 161 47 290 226 Fragments arranged by size: 552 290 278 226 161 47 AvaI C'yCGr_G Cuts at: 0 14 138 145 1554 Size: 14 124 7 1409 Fragments arranged by size: 1409 124 14 7 BanI G'GyrC_C Cuts at: 0 217 913 1222 1554 Size: 217 696 309 332 Fragments arranged by size: 696 332 309 217 BanII G_rGCy'C Cuts at: 0 1266 1294 1397 1554 Size: 1266 28 103 157 Fragments arranged by size: 1266 157 103 28 BbeI G_GCGC'C Cuts at: 0 221 1554 Size: 221 1333 BbsI GAAGACnn'nnnn_ Cuts at: 0 485 1554 Size: 485 1069 BbvCI CC'TCA_GC Cuts at: 0 1130 1554 Size: 1130 424 BmrI ACTGGGnnnn_n' Cuts at: 0 36 1022 1554 Size: 36 986 532 Fragments arranged by size: 986 532 36 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1462 1554 Size: 1462 92 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 211 301 652 1083 1554 Size: 211 90 351 431 471 Fragments arranged by size: 471 431 351 211 90 Bpu10I CC'TnA_GC Cuts at: 0 1130 1554 Size: 1130 424 BsaI GGTCTCn'nnnn_ Cuts at: 0 628 1554 Size: 628 926 BsaAI yAC'GTr Cuts at: 0 383 1554 Size: 383 1171 BsaBI GATnn'nnATC Cuts at: 0 289 1554 Size: 289 1265 BsaHI Gr'CG_yC Cuts at: 0 205 218 431 1554 Size: 205 13 213 1123 Fragments arranged by size: 1123 213 205 13 BsaWI w'CCGG_w Cuts at: 0 1481 1554 Size: 1481 73 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 667 697 1554 Size: 667 30 857 Fragments arranged by size: 857 667 30 BseYI C'CCAG_C Cuts at: 0 1019 1554 Size: 1019 535 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1319 1554 Size: 1319 235 Bsp1286I G_dGCh'C Cuts at: 0 1266 1294 1397 1554 Size: 1266 28 103 157 Fragments arranged by size: 1266 157 103 28 BspHI T'CATG_A Cuts at: 0 1424 1554 Size: 1424 130 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 257 1094 1300 1554 Size: 257 837 206 254 Fragments arranged by size: 837 257 254 206 BsrBI CCG'CTC Cuts at: 0 416 1378 1554 Size: 416 962 176 Fragments arranged by size: 962 416 176 BsrDI GCAATG_nn' Cuts at: 0 527 1554 Size: 527 1027 BsrFI r'CCGG_y Cuts at: 0 703 1159 1554 Size: 703 456 395 Fragments arranged by size: 703 456 395 BssHII G'CGCG_C Cuts at: 0 54 1554 Size: 54 1500 BstAPI GCAn_nnn'nTGC Cuts at: 0 1306 1315 1554 Size: 1306 9 239 Fragments arranged by size: 1306 239 9 BstXI CCAn_nnnn'nTGG Cuts at: 0 231 1233 1554 Size: 231 1002 321 Fragments arranged by size: 1002 321 231 BstYI r'GATC_y Cuts at: 0 721 1554 Size: 721 833 BstZ17I GTA'TAC Cuts at: 0 794 1554 Size: 794 760 Bsu36I CC'TnA_GG Cuts at: 0 1508 1554 Size: 1508 46 BtgI C'CryG_G Cuts at: 0 107 212 236 267 350 1226 1554 Size: 107 105 24 31 83 876 328 Fragments arranged by size: 876 328 107 105 83 31 24 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1528 1554 Size: 1528 26 BtsI GCAGTG_nn' Cuts at: 0 1325 1554 Size: 1325 229 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 522 557 1554 Size: 522 35 997 Fragments arranged by size: 997 522 35 DrdI GACnn_nn'nnGTC Cuts at: 0 1510 1554 Size: 1510 44 EaeI y'GGCC_r Cuts at: 0 104 110 233 264 1554 Size: 104 6 123 31 1290 Fragments arranged by size: 1290 123 104 31 6 EarI CTCTTCn'nnn_ Cuts at: 0 803 1264 1554 Size: 803 461 290 Fragments arranged by size: 803 461 290 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 211 301 652 1083 1554 Size: 211 90 351 431 471 Fragments arranged by size: 471 431 351 211 90 EcoO109I rG'GnC_Cy Cuts at: 0 142 149 296 1016 1554 Size: 142 7 147 720 538 Fragments arranged by size: 720 538 147 142 7 EcoRI G'AATT_C Cuts at: 0 991 1038 1554 Size: 991 47 516 Fragments arranged by size: 991 516 47 FseI GG_CCGG'CC Cuts at: 0 1163 1554 Size: 1163 391 HaeII r_GCGC'y Cuts at: 0 22 165 184 221 1554 Size: 22 143 19 37 1333 Fragments arranged by size: 1333 143 37 22 19 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1081 1113 1513 1545 1554 Size: 1081 32 400 32 9 Fragments arranged by size: 1081 400 32 32 9 HincII GTy'rAC Cuts at: 0 327 525 1554 Size: 327 198 1029 Fragments arranged by size: 1029 327 198 KasI G'GCGC_C Cuts at: 0 217 1554 Size: 217 1337 MscI TGG'CCA Cuts at: 0 235 1554 Size: 235 1319 MslI CAynn'nnrTG Cuts at: 0 691 1231 1554 Size: 691 540 323 Fragments arranged by size: 691 540 323 MspA1I CmG'CkG Cuts at: 0 109 214 269 1023 1554 Size: 109 105 55 754 531 Fragments arranged by size: 754 531 109 105 55 NaeI GCC'GGC Cuts at: 0 705 1161 1554 Size: 705 456 393 Fragments arranged by size: 705 456 393 NarI GG'CG_CC Cuts at: 0 218 1554 Size: 218 1336 NcoI C'CATG_G Cuts at: 0 236 1554 Size: 236 1318 NgoMIV G'CCGG_C Cuts at: 0 703 1159 1554 Size: 703 456 395 Fragments arranged by size: 703 456 395 PflMI CCAn_nnn'nTGG Cuts at: 0 1403 1554 Size: 1403 151 PpuMI rG'GwC_Cy Cuts at: 0 142 149 296 1016 1554 Size: 142 7 147 720 538 Fragments arranged by size: 720 538 147 142 7 PstI C_TGCA'G Cuts at: 0 254 877 1320 1554 Size: 254 623 443 234 Fragments arranged by size: 623 443 254 234 PvuII CAG'CTG Cuts at: 0 1023 1554 Size: 1023 531 SacII CC_GC'GG Cuts at: 0 110 215 270 1554 Size: 110 105 55 1284 Fragments arranged by size: 1284 110 105 55 SanDI GG'GwC_CC Cuts at: 0 142 149 296 1016 1554 Size: 142 7 147 720 538 Fragments arranged by size: 720 538 147 142 7 SbfI CC_TGCA'GG Cuts at: 0 254 1554 Size: 254 1300 SfcI C'TryA_G Cuts at: 0 250 313 873 1316 1554 Size: 250 63 560 443 238 Fragments arranged by size: 560 443 250 238 63 SfoI GGC'GCC Cuts at: 0 219 1554 Size: 219 1335 SmaI CCC'GGG Cuts at: 0 16 140 147 1554 Size: 16 124 7 1407 Fragments arranged by size: 1407 124 16 7 SrfI GCCC'GGGC Cuts at: 0 16 1554 Size: 16 1538 StyI C'CwwG_G Cuts at: 0 236 419 1051 1554 Size: 236 183 632 503 Fragments arranged by size: 632 503 236 183 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 742 1554 Size: 742 812 TatI w'GTAC_w Cuts at: 0 443 1554 Size: 443 1111 Tth111I GACn'n_nGTC Cuts at: 0 484 1014 1554 Size: 484 530 540 Fragments arranged by size: 540 530 484 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1209 1554 Size: 1209 345 XmaI C'CCGG_G Cuts at: 0 14 138 145 1554 Size: 14 124 7 1409 Fragments arranged by size: 1409 124 14 7 ZraI GAC'GTC Cuts at: 0 432 1554 Size: 432 1122 Enzymes that do cut: AarI AatII AccI AclI AflIII AlfI AlwNI ApoI AvaI BanI BanII BbeI BbsI BbvCI BmrI BplI BpmI Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssHII BstAPI BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI CspCI DrdI EaeI EarI Eco57MI EcoO109I EcoRI FseI HaeII Hin4I HincII KasI MscI MslI MspA1I NaeI NarI NcoI NgoMIV PflMI PpuMI PstI PvuII SacII SanDI SbfI SfcI SfoI SmaI SrfI StyI TaqII TatI Tth111I XcmI XmaI ZraI Enzymes that do not cut: Acc65I AcuI AfeI AflII AgeI AhdI AleI AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmtI BpuEI BseRI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BsrGI BssSI BstBI BstEII ClaI DraI DraIII EagI EciI EcoICRI EcoNI EcoRV FalI FspI FspAI HindIII HpaI KpnI MfeI MluI MmeI NdeI NheI NotI NruI NsiI NspI PacI PasI PciI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SalI SapI ScaI SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI SphI SspI StuI SwaI TstI XbaI XhoI XmnI