(Circular) (Six-base) MAPSORT of: pFN21AE6212.seq Check: 1513 from: 1 to: 5419 pFN21AE6212 5419 bp With 182 enzymes: SgfI * March 18, 2015 14:49 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3229 278 Size: 53 83 186 936 1693 2468 Fragments arranged by size: 2468 1693 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2762 1937 Size: 825 4594 Acc65I G'GTAC_C Cuts at: 2133 2740 2133 Size: 607 4812 AclI AA'CG_TT Cuts at: 1563 3546 3919 1563 Size: 1983 373 3063 Fragments arranged by size: 3063 1983 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3482 4866 5134 1632 Size: 1850 1384 268 1917 Fragments arranged by size: 1917 1850 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5222 AflIII A'CryG_T Cuts at: 1203 2127 2145 4318 5295 1203 Size: 924 18 2173 977 1327 Fragments arranged by size: 2173 1327 977 924 18 AgeI A'CCGG_T Cuts at: 4250 4250 Size: 5419 AhdI GACnn_n'nnGTC Cuts at: 4148 4148 Size: 5419 AleI CACnn'nnGTG Cuts at: 1166 2561 1166 Size: 1395 4024 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2526 1184 Size: 1342 4077 AlwNI CAG_nnn'CTG Cuts at: 3162 4734 3162 Size: 1572 3847 ApaLI G'TGCA_C Cuts at: 2303 2698 3476 4632 2303 Size: 395 778 1156 3090 Fragments arranged by size: 3090 1156 778 395 ApoI r'AATT_y Cuts at: 1493 2543 2728 2954 3106 5215 1493 Size: 1050 185 226 152 2109 1697 Fragments arranged by size: 2109 1697 1050 226 185 152 AseI AT'TA_AT Cuts at: 160 1032 3973 160 Size: 872 2941 1606 Fragments arranged by size: 2941 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5419 AvaI C'yCGr_G Cuts at: 1870 1943 2231 2369 2744 1870 Size: 73 288 138 375 4545 Fragments arranged by size: 4545 375 288 138 73 BamHI G'GATC_C Cuts at: 2749 3116 2749 Size: 367 5052 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2133 2740 4195 618 Size: 325 217 627 346 607 1455 1842 Fragments arranged by size: 1842 1455 627 607 346 325 217 BanII G_rGCy'C Cuts at: 1391 2232 2378 2386 2420 2738 1391 Size: 841 146 8 34 318 4072 Fragments arranged by size: 4072 841 318 146 34 8 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2383 928 Size: 971 484 3964 Fragments arranged by size: 3964 971 484 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2014 2048 3610 3644 1903 Size: 34 77 34 1562 34 3678 Fragments arranged by size: 3678 1562 77 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3311 4521 3311 Size: 1210 4209 BclI T'GATC_A Cuts at: 1548 1548 Size: 5419 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4030 136 Size: 107 122 71 631 605 2358 1525 Fragments arranged by size: 2358 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2100 5414 2100 Size: 3314 2105 BlpI GC'TnA_GC Cuts at: 2836 2836 Size: 5419 Bme1580I G_kGCm'C Cuts at: 1790 2307 2702 3480 4636 1790 Size: 517 395 778 1156 2573 Fragments arranged by size: 2573 1156 778 517 395 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5419 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3150 4108 442 Size: 416 53 83 399 24 1733 958 1753 Fragments arranged by size: 1753 1733 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5419 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2081 2261 2570 4079 1239 Size: 842 180 309 1509 2579 Fragments arranged by size: 2579 1509 842 309 180 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3544 4409 4707 3544 Size: 865 298 4256 Fragments arranged by size: 4256 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4082 882 Size: 617 2583 2219 Fragments arranged by size: 2583 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2128 2173 3210 493 Size: 711 924 45 1037 2702 Fragments arranged by size: 2702 1037 924 711 45 BsaBI GATnn'nnATC Cuts at: 5413 5413 Size: 5419 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2064 2646 3226 3608 Size: 53 83 186 936 531 582 580 382 Cuts at: 3608 4243 275 Size: 635 1451 Fragments arranged by size: 1451 936 635 582 580 531 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2477 3852 4250 4524 4671 5082 2477 Size: 1375 398 274 147 411 2814 Fragments arranged by size: 2814 1375 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5194 Fragments arranged by size: 5194 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5419 BseYI C'CCAG_C Cuts at: 2043 2152 2238 4622 5118 2043 Size: 109 86 2384 496 2344 Fragments arranged by size: 2384 2344 496 109 86 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2585 2594 1163 Size: 573 849 9 3988 Fragments arranged by size: 3988 849 573 9 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2438 2869 3630 4658 1540 Size: 275 186 437 431 761 1028 2301 Fragments arranged by size: 2301 1028 761 437 431 275 186 BsiHKAI G_wGCw'C Cuts at: 2307 2420 2702 3480 3565 4636 2307 Size: 113 282 778 85 1071 3090 Fragments arranged by size: 3090 1071 778 282 113 85 BsmI GAATG_Cn' Cuts at: 2930 3023 2930 Size: 93 5326 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2232 2307 2378 2386 2420 2702 2738 Size: 399 442 75 71 8 34 282 36 Cuts at: 2738 3480 3565 4636 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 442 399 282 85 75 71 36 34 8 BspEI T'CCGG_A Cuts at: 2477 5082 2477 Size: 2605 2814 BspHI T'CATG_A Cuts at: 3308 5038 3308 Size: 1730 3689 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2280 2774 844 Size: 1436 494 3489 Fragments arranged by size: 3489 1436 494 BsrBI CCG'CTC Cuts at: 1394 1823 2314 2622 3306 1394 Size: 429 491 308 684 3507 Fragments arranged by size: 3507 684 491 429 308 BsrDI GCAATG_nn' Cuts at: 65 1248 2015 2667 3914 4088 65 Size: 1183 767 652 1247 174 1396 Fragments arranged by size: 1396 1247 1183 767 652 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4063 4250 1700 Size: 110 2253 187 2869 Fragments arranged by size: 2869 2253 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5419 BssHII G'CGCG_C Cuts at: 1925 2243 1925 Size: 318 5101 BssSI C'ACGA_G Cuts at: 2291 3479 4491 2291 Size: 1188 1012 3219 Fragments arranged by size: 3219 1188 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2221 2572 794 Size: 1427 351 3641 Fragments arranged by size: 3641 1427 351 BstBI TT'CG_AA Cuts at: 2397 4223 2397 Size: 1826 3593 BstEII G'GTnAC_C Cuts at: 1184 2470 2485 1184 Size: 1286 15 4118 Fragments arranged by size: 4118 1286 15 BstYI r'GATC_y Cuts at: 1972 2100 2749 3116 3501 3518 4970 5056 5068 Size: 128 649 367 385 17 1452 86 12 Cuts at: 5068 5414 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 649 385 367 346 128 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2284 2589 513 Size: 552 1219 305 3343 Fragments arranged by size: 3343 1219 552 305 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2728 487 Size: 683 56 1502 3178 Fragments arranged by size: 3178 1502 683 56 BtsI GCAGTG_nn' Cuts at: 2935 3729 3749 2935 Size: 794 20 4605 Fragments arranged by size: 4605 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5419 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5384 DraI TTT'AAA Cuts at: 2723 2723 Size: 5419 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2468 4426 809 Size: 507 1152 1958 1802 Fragments arranged by size: 1958 1802 1152 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2435 2866 3755 Size: 54 1425 50 275 18 605 431 889 Cuts at: 3755 5384 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 605 431 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2435 2866 1537 Size: 275 623 431 4090 Fragments arranged by size: 4090 623 431 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3349 1353 Size: 1996 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4006 4380 4526 387 Size: 1064 138 2417 374 146 1280 Fragments arranged by size: 2417 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2418 2418 Size: 5419 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2081 2261 2570 3482 4079 4866 5134 Size: 393 449 180 309 912 597 787 268 Cuts at: 5134 1239 Size: 1524 Fragments arranged by size: 1524 912 787 597 449 393 309 268 180 EcoRI G'AATT_C Cuts at: 2543 2728 3106 2543 Size: 185 378 4856 Fragments arranged by size: 4856 378 185 FspI TGC'GCA Cuts at: 3925 3925 Size: 5419 HaeII r_GCGC'y Cuts at: 1678 4566 1678 Size: 2888 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2373 2405 4060 4092 4134 Size: 32 135 32 801 32 1655 32 42 Cuts at: 4134 4166 1373 Size: 32 2626 Fragments arranged by size: 2626 1655 801 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2010 2763 3009 669 Size: 988 281 72 753 246 3079 Fragments arranged by size: 3079 988 753 281 246 72 HindIII A'AGCT_T Cuts at: 748 748 Size: 5419 HpaI GTT'AAC Cuts at: 3009 3009 Size: 5419 KpnI G_GTAC'C Cuts at: 2137 2744 2137 Size: 607 4812 MfeI C'AATT_G Cuts at: 3018 3018 Size: 5419 MluI A'CGCG_T Cuts at: 2145 5295 2145 Size: 3150 2269 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4533 4717 1305 Size: 3228 184 2007 Fragments arranged by size: 3228 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3597 Fragments arranged by size: 3597 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2561 3377 3736 3895 518 Size: 648 1395 816 359 159 2042 Fragments arranged by size: 2042 1395 816 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2212 2343 2591 2835 3512 4660 4905 1606 Size: 606 131 248 244 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 606 248 245 244 131 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4867 NdeI CA'TA_TG Cuts at: 387 2091 387 Size: 1704 3715 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5419 NotI GC'GGCC_GC Cuts at: 2866 2866 Size: 5419 NspI r_CATG'y Cuts at: 1132 1229 2777 4322 1132 Size: 97 1548 1545 2229 Fragments arranged by size: 2229 1548 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5419 PciI A'CATG_T Cuts at: 4318 4318 Size: 5419 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5419 PfoI T'CCnGG_A Cuts at: 2547 2547 Size: 5419 PmeI GTTT'AAAC Cuts at: 2723 2723 Size: 5419 PmlI CAC'GTG Cuts at: 2128 2173 2128 Size: 45 5374 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3488 3520 5001 5033 3488 Size: 32 1481 32 3874 Fragments arranged by size: 3874 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5419 PsiI TTA'TAA Cuts at: 2989 2989 Size: 5419 PstI C_TGCA'G Cuts at: 830 2217 2265 2502 2771 830 Size: 1387 48 237 269 3478 Fragments arranged by size: 3478 1387 269 237 48 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5419 PvuII CAG'CTG Cuts at: 2212 2212 Size: 5419 SacI G_AGCT'C Cuts at: 2420 2420 Size: 5419 SacII CC_GC'GG Cuts at: 2592 2592 Size: 5419 SalI G'TCGA_C Cuts at: 1936 2761 1936 Size: 825 4594 SbfI CC_TGCA'GG Cuts at: 2771 2771 Size: 5419 ScaI AGT'ACT Cuts at: 3667 5379 3667 Size: 1712 3707 SfcI C'TryA_G Cuts at: 826 1045 2213 2261 2498 2682 2767 3902 4583 Size: 219 1168 48 237 184 85 1135 681 Cuts at: 4583 4774 826 Size: 191 1471 Fragments arranged by size: 1471 1168 1135 681 237 219 191 184 85 48 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5419 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5419 SmaI CCC'GGG Cuts at: 1872 2746 1872 Size: 874 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3523 4424 4686 820 Size: 197 926 1580 901 262 1553 Fragments arranged by size: 1580 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3210 493 Size: 2717 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5419 SphI G_CATG'C Cuts at: 1132 2777 1132 Size: 1645 3774 SspI AAT'ATT Cuts at: 5 52 3343 5 Size: 47 3291 2081 Fragments arranged by size: 3291 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5419 StyI C'CwwG_G Cuts at: 513 1065 2319 2858 513 Size: 552 1254 539 3074 Fragments arranged by size: 3074 1254 552 539 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2480 3446 3463 3616 3801 4246 325 Size: 1255 900 966 17 153 185 445 1498 Fragments arranged by size: 1498 1255 966 900 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3665 5377 96 Size: 274 80 33 51 1443 1688 1712 138 Fragments arranged by size: 1712 1688 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5379 5411 5379 Size: 32 5387 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5419 XbaI T'CTAG_A Cuts at: 2378 2755 2378 Size: 377 5042 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5419 XmaI C'CCGG_G Cuts at: 1870 2744 1870 Size: 874 4545 XmnI GAAnn'nnTTC Cuts at: 3548 3548 Size: 5419 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3227 276 Size: 53 83 186 936 1693 2468 Fragments arranged by size: 2468 1693 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI PsrI RsrII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI