################################## # NO CUT ENZYMES ( 144 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BclI BfrI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiHKAI BsiWI BsmBI BsmI BsoBI BspDI BspEI BspLU11I BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII CfoI ClaI CpoI DraIII DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV EcoT14I EcoT22I FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MunI MvnI NarI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I StuI StyI SwaI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AccI 394 GTCTAC GT^[AC][GT]AC AlwNI 980 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 374 GGGCCC GGGCC^C Asp700I 148 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BanII 374 GGGCCC G[GA]GC[CT]^C BcgI 522 CGACGTGATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 162 GGATAC GTATCC(6/5) BfaI 451 CTAG C^TAG BglI 1036 GCCTTATTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BmyI 374 GGGCCC G[AGT]GC[ACT]^C BsaAI 244 TACGTG [CT]AC^GT[GA] BsiEI 411 CGGTCG CG[GA][CT]^CG BsmAI 359 GTCTC GTCTC(1/5) Bsp1286I 374 GGGCCC G[AGT]GC[ACT]^C Bsp1407I 1274 TGTACA T^GTACA BspHI 461 TCATGA T^CATGA BspMI 1102 GCAGGT ACCTGC(4/8) BsrBI 78 GAGCGG CCGCTC(-3/-3) BsrGI 1274 TGTACA T^GTACA BstAPI 976 GCAGCAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 1413 AGATCC [GA]^GATC[CT] DrdI 580 GACATTGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRI 559 GAATTC G^AATTC HincII 603 GTTAAC GT[CT]^[GA]AC HindII 603 GTTAAC GT[CT]^[GA]AC HindIII 326 AAGCTT A^AGCTT HpaI 603 GTTAAC GTT^AAC HphI 4 TCACC GGTGA(8/7) MaeI 451 CTAG C^TAG MflI 1413 AGATCC [GA]^GATC[CT] NaeI 32 GCCGGC GCC^GGC NgoMIV 32 GCCGGC G^CCGGC NspI 1280 GCATGT [GA]CATG^[CT] PflMI 1324 CCACTTATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 1104 AGGTCCC [GA]G^G[AT]CC[CT] PstI 1100 CTGCAG CTGCA^G RcaI 461 TCATGA T^CATGA SspBI 1274 TGTACA T^GTACA SspI 728 AATATT AAT^ATT Van91I 1324 CCACTTATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 617 CCAGGGAAAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 1413 AGATCC [GA]^GATC[CT] XmnI 148 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AfaI 1018 GTAC GT^AC 1275 GTAC BcnI 132 CCGGG CC^[GC]GG 204 CCGGG BsrDI 582 CATTGC GCAATG(2/0) 683 GCAATG BsrFI 32 GCCGGC [GA]^CCGG[CT] 166 ACCGGC BstXI 94 CCAGGCGGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 476 CCACTCAGGTGG Cfr10I 32 GCCGGC [GA]^CCGG[CT] 166 ACCGGC DraI 1353 TTTAAA TTT^AAA 1594 TTTAAA DraII 374 GGGCCCC [GA]G^G[ACGT]CC[CT] 1104 AGGTCCC EaeI 43 CGGCCA [CT]^GGCC[GA] 566 TGGCCG EcoO109I 374 GGGCCCC [GA]G^G[ACGT]CC[CT] 1104 AGGTCCC MslI 477 CACTCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1619 CATCTTCGTG MspA1I 125 CAGCGG C[AC]G^C[GT]G 665 CAGCGG NciI 132 CCGGG CC^[GC]GG 204 CCGGG RsaI 1018 GTAC GT^AC 1275 GTAC SapI 75 GAAGAGC GCTCTTC(1/4) 151 GCTCTTC TaqI 590 TCGA T^CGA 1461 TCGA Tsp45I 3 GTCAC ^GT[GC]AC 59 GTCAC TthHB8I 590 TCGA T^CGA 1461 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 53 enzymes) # ######################################### AciI AcsI AluI AlwI ApoI AvaII BbvI BsaJI BsiYI BslI BsmFI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EarI EcoRII FauI Fnu4HI FokI HaeIII HapII HinfI HpaII ItaI Ksp632I MaeII MaeIII MboI MboII MnlI MseI MspI MvaI MwoI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI SfaNI SfcI TfiI Tru9I TseI Tsp509I TspRI