(Circular) (Six-base) MAPSORT of: pFN21AE6416.seq Check: 31 from: 1 to: 6352 pFN21AE6416 6352 bp With 182 enzymes: SgfI * March 18, 2015 14:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4162 278 Size: 53 83 186 936 2626 2468 Fragments arranged by size: 2626 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2400 3695 1937 Size: 463 1295 4594 Fragments arranged by size: 4594 1295 463 Acc65I G'GTAC_C Cuts at: 3673 3673 Size: 6352 AclI AA'CG_TT Cuts at: 1563 4479 4852 1563 Size: 2916 373 3063 Fragments arranged by size: 3063 2916 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2240 2323 2344 4415 5799 6067 1632 Size: 608 83 21 2071 1384 268 1917 Fragments arranged by size: 2071 1917 1384 608 268 83 21 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6155 AflIII A'CryG_T Cuts at: 1203 5251 6228 1203 Size: 4048 977 1327 Fragments arranged by size: 4048 1327 977 AgeI A'CCGG_T Cuts at: 5183 5183 Size: 6352 AhdI GACnn_n'nnGTC Cuts at: 5081 5081 Size: 6352 AleI CACnn'nnGTG Cuts at: 1166 2486 1166 Size: 1320 5032 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2246 1184 Size: 1062 5290 AlwNI CAG_nnn'CTG Cuts at: 2990 4095 5667 2990 Size: 1105 1572 3675 Fragments arranged by size: 3675 1572 1105 ApaI G_GGCC'C Cuts at: 2383 2383 Size: 6352 ApaLI G'TGCA_C Cuts at: 4409 5565 4409 Size: 1156 5196 ApoI r'AATT_y Cuts at: 1493 2056 2564 3596 3661 3887 4039 6148 1493 Size: 563 508 1032 65 226 152 2109 1697 Fragments arranged by size: 2109 1697 1032 563 508 226 152 65 AseI AT'TA_AT Cuts at: 160 1032 4906 160 Size: 872 3874 1606 Fragments arranged by size: 3874 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6352 AvaI C'yCGr_G Cuts at: 1870 1943 3677 1870 Size: 73 1734 4545 Fragments arranged by size: 4545 1734 73 BamHI G'GATC_C Cuts at: 3682 4049 3682 Size: 367 5985 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3673 5128 618 Size: 325 217 627 1886 1455 1842 Fragments arranged by size: 1886 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2383 3671 1391 Size: 992 1288 4072 Fragments arranged by size: 4072 1288 992 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5381 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2516 2550 4543 4577 1903 Size: 34 579 34 1993 34 3678 Fragments arranged by size: 3678 1993 579 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2161 4244 5454 2161 Size: 2083 1210 3059 Fragments arranged by size: 3059 2083 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6352 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3047 4963 136 Size: 107 122 71 631 605 1375 1916 1525 Fragments arranged by size: 1916 1525 1375 631 605 122 107 71 BglII A'GATC_T Cuts at: 6347 6347 Size: 6352 BlpI GC'TnA_GC Cuts at: 3769 3769 Size: 6352 Bme1580I G_kGCm'C Cuts at: 1790 2383 4413 5569 1790 Size: 593 2030 1156 2573 Fragments arranged by size: 2573 2030 1156 593 BmgBI CAC'GTC Cuts at: 1318 2530 1318 Size: 1212 5140 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3509 4083 5041 Size: 416 53 83 399 24 2092 574 958 Cuts at: 5041 442 Size: 1753 Fragments arranged by size: 2092 1753 958 574 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6352 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5012 1239 Size: 3773 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4477 5342 5640 4477 Size: 865 298 5189 Fragments arranged by size: 5189 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5015 882 Size: 617 3516 2219 Fragments arranged by size: 3516 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2251 4143 493 Size: 711 1047 1892 2702 Fragments arranged by size: 2702 1892 1047 711 BsaBI GATnn'nnATC Cuts at: 6346 6346 Size: 6352 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4159 4541 5176 275 Size: 53 83 186 936 2626 382 635 1451 Fragments arranged by size: 2626 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4785 5183 5457 5604 6015 4785 Size: 398 274 147 411 5122 Fragments arranged by size: 5122 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6127 Fragments arranged by size: 6127 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6352 BseYI C'CCAG_C Cuts at: 3114 5555 6051 3114 Size: 2441 496 3415 Fragments arranged by size: 3415 2441 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5779 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2419 3802 4563 5591 1540 Size: 275 186 418 1383 761 1028 2301 Fragments arranged by size: 2301 1383 1028 761 418 275 186 BsiHKAI G_wGCw'C Cuts at: 4413 4498 5569 4413 Size: 85 1071 5196 Fragments arranged by size: 5196 1071 85 BsmI GAATG_Cn' Cuts at: 3863 3956 3863 Size: 93 6259 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2383 3671 4413 4498 5569 1391 Size: 399 593 1288 742 85 1071 2174 Fragments arranged by size: 2174 1288 1071 742 593 399 85 BspEI T'CCGG_A Cuts at: 6015 6015 Size: 6352 BspHI T'CATG_A Cuts at: 2466 4241 5971 2466 Size: 1775 1730 2847 Fragments arranged by size: 2847 1775 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3098 3707 844 Size: 2254 609 3489 Fragments arranged by size: 3489 2254 609 BsrBI CCG'CTC Cuts at: 1394 1823 2085 4239 1394 Size: 429 262 2154 3507 Fragments arranged by size: 3507 2154 429 262 BsrDI GCAATG_nn' Cuts at: 65 1248 2586 2695 4847 5021 65 Size: 1183 1338 109 2152 174 1396 Fragments arranged by size: 2152 1396 1338 1183 174 109 BsrFI r'CCGG_y Cuts at: 1700 1810 2037 2171 4996 5183 1700 Size: 110 227 134 2825 187 2869 Fragments arranged by size: 2869 2825 227 187 134 110 BsrGI T'GTAC_A Cuts at: 96 3279 96 Size: 3183 3169 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6352 BssSI C'ACGA_G Cuts at: 4412 5424 4412 Size: 1012 5340 BstAPI GCAn_nnn'nTGC Cuts at: 794 2987 794 Size: 2193 4159 BstBI TT'CG_AA Cuts at: 5156 5156 Size: 6352 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6352 BstXI CCAn_nnnn'nTGG Cuts at: 2106 2488 2106 Size: 382 5970 BstYI r'GATC_y Cuts at: 1972 3418 3682 4049 4434 4451 5903 5989 6001 Size: 1446 264 367 385 17 1452 86 12 Cuts at: 6001 6347 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1446 385 367 346 264 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5800 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5613 Fragments arranged by size: 5613 683 56 BtsI GCAGTG_nn' Cuts at: 3102 3868 4662 4682 3102 Size: 766 794 20 4772 Fragments arranged by size: 4772 794 766 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6352 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2280 2315 529 Size: 35 1716 35 4566 Fragments arranged by size: 4566 1716 35 35 DraI TTT'AAA Cuts at: 3360 3601 3656 3360 Size: 241 55 6056 Fragments arranged by size: 6056 241 55 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2591 5359 809 Size: 507 1275 2768 1802 Fragments arranged by size: 2768 1802 1275 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2048 2571 3799 Size: 54 1425 50 275 18 218 523 1228 Cuts at: 3799 4688 6317 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1228 889 523 275 218 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3799 1537 Size: 275 1987 4090 Fragments arranged by size: 4090 1987 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2075 2163 2468 3009 4282 1353 Size: 722 88 305 541 1273 3423 Fragments arranged by size: 3423 1273 722 541 305 88 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4939 5313 5459 387 Size: 1064 138 3350 374 146 1280 Fragments arranged by size: 3350 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2240 2323 2344 4415 5012 5799 6067 Size: 393 608 83 21 2071 597 787 268 Cuts at: 6067 1239 Size: 1524 Fragments arranged by size: 2071 1524 787 608 597 393 268 83 21 EcoO109I rG'GnC_Cy Cuts at: 2380 3110 2380 Size: 730 5622 EcoRI G'AATT_C Cuts at: 2564 3661 4039 2564 Size: 1097 378 4877 Fragments arranged by size: 4877 1097 378 FspI TGC'GCA Cuts at: 4858 4858 Size: 6352 HaeII r_GCGC'y Cuts at: 1678 5499 1678 Size: 3821 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3605 3637 4993 5025 5067 Size: 32 135 32 2033 32 1356 32 42 Cuts at: 5067 5099 1373 Size: 32 2626 Fragments arranged by size: 2626 2033 1356 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2610 3696 3942 669 Size: 988 281 672 1086 246 3079 Fragments arranged by size: 3079 1086 988 672 281 246 HindIII A'AGCT_T Cuts at: 748 2331 748 Size: 1583 4769 HpaI GTT'AAC Cuts at: 2610 3942 2610 Size: 1332 5020 KpnI G_GTAC'C Cuts at: 3677 3677 Size: 6352 MfeI C'AATT_G Cuts at: 3951 3951 Size: 6352 MluI A'CGCG_T Cuts at: 6228 6228 Size: 6352 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5466 5650 1305 Size: 4161 184 2007 Fragments arranged by size: 4161 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4530 Fragments arranged by size: 4530 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2486 3628 4310 4669 4828 518 Size: 648 1320 1142 682 359 159 2042 Fragments arranged by size: 2042 1320 1142 682 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2132 2672 3768 4445 5593 5838 1606 Size: 526 540 1096 677 1148 245 2120 Fragments arranged by size: 2120 1148 1096 677 540 526 245 NaeI GCC'GGC Cuts at: 2039 2039 Size: 6352 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5800 NdeI CA'TA_TG Cuts at: 387 387 Size: 6352 NgoMIV G'CCGG_C Cuts at: 2037 2037 Size: 6352 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6352 NotI GC'GGCC_GC Cuts at: 3799 3799 Size: 6352 NspI r_CATG'y Cuts at: 1132 1229 3289 3710 5255 1132 Size: 97 2060 421 1545 2229 Fragments arranged by size: 2229 2060 1545 421 97 PasI CC'CwG_GG Cuts at: 1348 2269 1348 Size: 921 5431 PciI A'CATG_T Cuts at: 5251 5251 Size: 6352 PflMI CCAn_nnn'nTGG Cuts at: 1108 3335 1108 Size: 2227 4125 PmeI GTTT'AAAC Cuts at: 3656 3656 Size: 6352 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4421 4453 5934 5966 4421 Size: 32 1481 32 4807 Fragments arranged by size: 4807 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3110 3110 Size: 6352 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6352 PsiI TTA'TAA Cuts at: 2918 3922 2918 Size: 1004 5348 PspOMI G'GGCC_C Cuts at: 2379 2379 Size: 6352 PstI C_TGCA'G Cuts at: 830 3109 3704 830 Size: 2279 595 3478 Fragments arranged by size: 3478 2279 595 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6352 SalI G'TCGA_C Cuts at: 1936 3694 1936 Size: 1758 4594 SapI GCTCTTCn'nnn_ Cuts at: 2075 2163 2075 Size: 88 6264 SbfI CC_TGCA'GG Cuts at: 3704 3704 Size: 6352 ScaI AGT'ACT Cuts at: 4600 6312 4600 Size: 1712 4640 SfcI C'TryA_G Cuts at: 826 1045 3105 3148 3396 3700 4835 5516 5707 Size: 219 2060 43 248 304 1135 681 191 Cuts at: 5707 826 Size: 1471 Fragments arranged by size: 2060 1471 1135 681 304 248 219 191 43 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6352 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6352 SmaI CCC'GGG Cuts at: 1872 3679 1872 Size: 1807 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4456 5357 5619 820 Size: 197 926 2513 901 262 1553 Fragments arranged by size: 2513 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4143 493 Size: 3650 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6352 SphI G_CATG'C Cuts at: 1132 3710 1132 Size: 2578 3774 SspI AAT'ATT Cuts at: 5 52 2735 4276 5 Size: 47 2683 1541 2081 Fragments arranged by size: 2683 2081 1541 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6352 StyI C'CwwG_G Cuts at: 513 1065 3791 513 Size: 552 2726 3074 Fragments arranged by size: 3074 2726 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2026 2405 3491 4379 4396 4549 4734 Size: 1255 446 379 1086 888 17 153 185 Cuts at: 4734 5179 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1086 888 446 445 379 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3279 4598 6310 Size: 274 80 33 51 1443 1302 1319 1712 Cuts at: 6310 96 Size: 138 Fragments arranged by size: 1712 1443 1319 1302 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2190 2222 3094 3126 6312 6344 2190 Size: 32 872 32 3186 32 2198 Fragments arranged by size: 3186 2198 872 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6352 XbaI T'CTAG_A Cuts at: 3688 3688 Size: 6352 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2629 2629 Size: 6352 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6352 XmaI C'CCGG_G Cuts at: 1870 3677 1870 Size: 1807 4545 XmnI GAAnn'nnTTC Cuts at: 2157 4481 2157 Size: 2324 4028 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4160 276 Size: 53 83 186 936 2626 2468 Fragments arranged by size: 2626 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI