(Linear) (Six-base) MAPSORT of: KIEE6416.seq Check: 5506 from: 1 to: 1647 KIEE6416.orf With 182 enzymes: SgfI * March 16, 2015 11:31 .. AccI GT'mk_AC Cuts at: 0 395 1647 Size: 395 1252 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 235 318 339 1647 Size: 235 83 21 1308 Fragments arranged by size: 1308 235 83 21 AleI CACnn'nnGTG Cuts at: 0 481 1647 Size: 481 1166 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 241 1647 Size: 241 1406 AlwNI CAG_nnn'CTG Cuts at: 0 985 1647 Size: 985 662 ApaI G_GGCC'C Cuts at: 0 378 1647 Size: 378 1269 ApoI r'AATT_y Cuts at: 0 51 559 1591 1647 Size: 51 508 1032 56 Fragments arranged by size: 1032 508 56 51 BanII G_rGCy'C Cuts at: 0 378 1647 Size: 378 1269 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 511 545 1647 Size: 511 34 1102 Fragments arranged by size: 1102 511 34 BciVI GTATCCnnnnn_n' Cuts at: 0 156 1647 Size: 156 1491 BglI GCCn_nnn'nGGC Cuts at: 0 1042 1647 Size: 1042 605 Bme1580I G_kGCm'C Cuts at: 0 378 1647 Size: 378 1269 BmgBI CAC'GTC Cuts at: 0 525 1647 Size: 525 1122 BmrI ACTGGGnnnn_n' Cuts at: 0 1504 1647 Size: 1504 143 BsaAI yAC'GTr Cuts at: 0 246 1647 Size: 246 1401 BseYI C'CCAG_C Cuts at: 0 1109 1647 Size: 1109 538 BsiEI CG_ry'CG Cuts at: 0 414 1647 Size: 414 1233 Bsp1286I G_dGCh'C Cuts at: 0 378 1647 Size: 378 1269 BspHI T'CATG_A Cuts at: 0 461 1647 Size: 461 1186 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1093 1647 Size: 1093 554 BsrBI CCG'CTC Cuts at: 0 80 1647 Size: 80 1567 BsrDI GCAATG_nn' Cuts at: 0 581 690 1647 Size: 581 109 957 Fragments arranged by size: 957 581 109 BsrFI r'CCGG_y Cuts at: 0 32 166 1647 Size: 32 134 1481 Fragments arranged by size: 1481 134 32 BsrGI T'GTAC_A Cuts at: 0 1274 1647 Size: 1274 373 BstAPI GCAn_nnn'nTGC Cuts at: 0 982 1647 Size: 982 665 BstXI CCAn_nnnn'nTGG Cuts at: 0 101 483 1647 Size: 101 382 1164 Fragments arranged by size: 1164 382 101 BstYI r'GATC_y Cuts at: 0 1413 1647 Size: 1413 234 BtsI GCAGTG_nn' Cuts at: 0 1097 1647 Size: 1097 550 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 275 310 1647 Size: 275 35 1337 Fragments arranged by size: 1337 275 35 DraI TTT'AAA Cuts at: 0 1355 1596 1647 Size: 1355 241 51 Fragments arranged by size: 1355 241 51 DrdI GACnn_nn'nnGTC Cuts at: 0 586 1647 Size: 586 1061 EaeI y'GGCC_r Cuts at: 0 43 566 1647 Size: 43 523 1081 Fragments arranged by size: 1081 523 43 EarI CTCTTCn'nnn_ Cuts at: 0 70 158 463 1004 1647 Size: 70 88 305 541 643 Fragments arranged by size: 643 541 305 88 70 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 235 318 339 1647 Size: 235 83 21 1308 Fragments arranged by size: 1308 235 83 21 EcoO109I rG'GnC_Cy Cuts at: 0 375 1105 1647 Size: 375 730 542 Fragments arranged by size: 730 542 375 EcoRI G'AATT_C Cuts at: 0 559 1647 Size: 559 1088 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1600 1632 1647 Size: 1600 32 15 Fragments arranged by size: 1600 32 15 HincII GTy'rAC Cuts at: 0 605 1647 Size: 605 1042 HindIII A'AGCT_T Cuts at: 0 326 1647 Size: 326 1321 HpaI GTT'AAC Cuts at: 0 605 1647 Size: 605 1042 MslI CAynn'nnrTG Cuts at: 0 481 1623 1647 Size: 481 1142 24 Fragments arranged by size: 1142 481 24 MspA1I CmG'CkG Cuts at: 0 127 667 1647 Size: 127 540 980 Fragments arranged by size: 980 540 127 NaeI GCC'GGC Cuts at: 0 34 1647 Size: 34 1613 NgoMIV G'CCGG_C Cuts at: 0 32 1647 Size: 32 1615 NspI r_CATG'y Cuts at: 0 1284 1647 Size: 1284 363 PasI CC'CwG_GG Cuts at: 0 264 1647 Size: 264 1383 PflMI CCAn_nnn'nTGG Cuts at: 0 1330 1647 Size: 1330 317 PpuMI rG'GwC_Cy Cuts at: 0 1105 1647 Size: 1105 542 PsiI TTA'TAA Cuts at: 0 913 1647 Size: 913 734 PspOMI G'GGCC_C Cuts at: 0 374 1647 Size: 374 1273 PstI C_TGCA'G Cuts at: 0 1104 1647 Size: 1104 543 SapI GCTCTTCn'nnn_ Cuts at: 0 70 158 1647 Size: 70 88 1489 Fragments arranged by size: 1489 88 70 SfcI C'TryA_G Cuts at: 0 1100 1143 1391 1647 Size: 1100 43 248 256 Fragments arranged by size: 1100 256 248 43 SspI AAT'ATT Cuts at: 0 730 1647 Size: 730 917 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 21 400 1486 1647 Size: 21 379 1086 161 Fragments arranged by size: 1086 379 161 21 TatI w'GTAC_w Cuts at: 0 1274 1647 Size: 1274 373 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 185 217 1089 1121 1647 Size: 185 32 872 32 526 Fragments arranged by size: 872 526 185 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 624 1647 Size: 624 1023 XmnI GAAnn'nnTTC Cuts at: 0 152 1647 Size: 152 1495 Enzymes that do cut: AccI AcuI AleI AlfI AlwNI ApaI ApoI BanII BcgI BciVI BglI Bme1580I BmgBI BmrI BsaAI BseYI BsiEI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BstAPI BstXI BstYI BtsI CspCI DraI DrdI EaeI EarI Eco57MI EcoO109I EcoRI Hin4I HincII HindIII HpaI MslI MspA1I NaeI NgoMIV NspI PasI PflMI PpuMI PsiI PspOMI PstI SapI SfcI SspI TaqII TatI TstI XcmI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI AloI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbsI BbvCI BclI BfrBI BglII BlpI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI BsgI BsiHKAI BsiWI BsmI BsmBI BspEI BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgI BtgZI ClaI DraIII EagI EciI EcoICRI EcoNI EcoRV FalI FseI FspI FspAI HaeII KasI KpnI MfeI MluI MmeI MscI NarI NcoI NdeI NheI NotI NruI NsiI PacI PciI PfoI PmeI PmlI PpiI PshAI PspXI PsrI PvuI PvuII RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI StuI StyI SwaI Tth111I XbaI XhoI XmaI ZraI