################################## # NO CUT ENZYMES ( 137 enzymes) # ################################## Acc65I AccI AccIII AclI AflII AflIII AgeI AhdI AlwI AocI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvrII BalI BamHI BbrPI BbsI BbvCI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaWI BseAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrFI BsrGI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DpnII DraI DrdI Eam1105I Eco47III Eco81I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI HincII HindII HindIII HinfI HpaI KpnI MamI MboI MfeI MflI MluI MluNI MroI MscI MseI MslI MunI NaeI NcoI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspV PacI PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SalI Sau3AI SbfI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI StyI SwaI TfiI Tru9I Tth111I Van91I XbaI XcmI XhoII XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AatII 364 GACGTC GACGT^C AcsI 229 GAATTT [GA]^AATT[CT] AfaI 515 GTAC GT^AC Alw44I 379 GTGCAC G^TGCAC ApaLI 379 GTGCAC G^TGCAC ApoI 229 GAATTT [GA]^AATT[CT] AvaII 535 GGTCC G^G[AT]CC AviII 1061 TGCGCA TGC^GCA BanI 636 GGCGCC G^G[CT][GA]CC BbeI 636 GGCGCC GGCGC^C BcgI 928 GCAGCCGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 931 GCCGCTTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaI 68 GAGACC GGTCTC(1/5) BsgI 592 CTGCAC GTGCAG(16/14) BsiEI 938 CGGCCG CG[GA][CT]^CG BsmAI 68 GAGAC GTCTC(1/5) BsrBI 343 CCGCTC CCGCTC(-3/-3) BssSI 1114 CTCGTG CACGAG(-5/-1) DraIII 1021 CACTCGGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 938 CGGCCG C^GGCCG EarI 346 CTCTTC CTCTTC(1/4) EclXI 938 CGGCCG C^GGCCG Eco52I 938 CGGCCG C^GGCCG EcoNI 987 CCTGCGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FspI 1061 TGCGCA TGC^GCA HphI 1014 GGTGA GGTGA(8/7) KasI 636 GGCGCC G^GCGCC Ksp632I 346 CTCTTC CTCTTC(1/4) KspI 330 CCGCGG CCGC^GG MaeII 365 ACGT A^CGT MaeIII 799 GTGAC ^GT[ACGT]AC NarI 636 GGCGCC GG^CGCC NlaIII 174 CATG CATG^ NspI 173 GCATGC [GA]CATG^[CT] PpuMI 534 GGGTCCC [GA]G^G[AT]CC[CT] PshAI 801 GACGCGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 477 CAGCTG CAG^CTG RsaI 515 GTAC GT^AC SacI 487 GAGCTC GAGCT^C SacII 330 CCGCGG CCGC^GG SapI 345 GCTCTTC GCTCTTC(1/4) SfoI 636 GGCGCC GGC^GCC SmaI 300 CCCGGG CCC^GGG SphI 173 GCATGC GCATG^C Tsp45I 799 GTGAC ^GT[GC]AC Tsp509I 230 AATT ^AATT XmaCI 300 CCCGGG C^CCGGG XmaI 300 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AlwNI 87 CAGGGTCTG CAG[ACGT][ACGT][ACGT]^CTG 96 CAGGGCCTG BanII 487 GAGCTC G[GA]GC[CT]^C 775 GAGCCC BfaI 520 CTAG C^TAG 598 CTAG BseRI 28 GAGGAG GAGGAG(10/8) 961 GAGGAG BspMI 118 ACCTGC ACCTGC(4/8) 422 ACCTGC BstF5I 618 CATCC GGATG(2/0) 1096 CATCC DraII 97 AGGGCCT [GA]G^G[ACGT]CC[CT] 534 GGGTCCC DsaI 330 CCGCGG C^C[GA][CT]GG 827 CCGTGG EcoO109I 97 AGGGCCT [GA]G^G[ACGT]CC[CT] 534 GGGTCCC FokI 618 CATCC GGATG(9/13) 1096 CATCC MaeI 520 CTAG C^TAG 598 CTAG PaeR7I 550 CTCGAG C^TCGAG 916 CTCGAG SfaNI 691 GATGC GCATC(5/9) 1095 GCATC XhoI 550 CTCGAG C^TCGAG 916 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 59 enzymes) # ######################################### AccII AciI AcyI AluI AspHI AvaI BbvI BcnI BmyI BpmI BsaHI BsaJI BsiHKAI BsiYI BslI BsmFI BsoBI Bsp1286I BsrI BssHII BssKI BstNI BstUI Cac8I CfoI Cfr13I DdeI EaeI EcoRII FauI Fnu4HI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII ItaI MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NlaIV PstI Sau96I ScrFI SfcI SmlI StuI TaqI TseI TspRI TthHB8I