(Circular) (Six-base) MAPSORT of: pFN21AE3943.seq Check: 3584 from: 1 to: 5827 pFN21AE3943 5827 bp With 182 enzymes: SgfI * March 28, 2012 11:49 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2373 3637 278 Size: 53 83 186 936 837 1264 2468 Fragments arranged by size: 2468 1264 936 837 186 83 53 AccI GT'mk_AC Cuts at: 1937 3170 1937 Size: 1233 4594 Acc65I G'GTAC_C Cuts at: 3148 3148 Size: 5827 AclI AA'CG_TT Cuts at: 1563 3954 4327 1563 Size: 2391 373 3063 Fragments arranged by size: 3063 2391 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3890 5274 5542 1632 Size: 2258 1384 268 1917 Fragments arranged by size: 2258 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5630 AflIII A'CryG_T Cuts at: 1203 4726 5703 1203 Size: 3523 977 1327 Fragments arranged by size: 3523 1327 977 AgeI A'CCGG_T Cuts at: 4658 4658 Size: 5827 AhdI GACnn_n'nnGTC Cuts at: 4556 4556 Size: 5827 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5827 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2929 1184 Size: 1745 4082 AlwNI CAG_nnn'CTG Cuts at: 2097 2106 3570 5142 2097 Size: 9 1464 1572 2782 Fragments arranged by size: 2782 1572 1464 9 ApaLI G'TGCA_C Cuts at: 2384 3884 5040 2384 Size: 1500 1156 3171 Fragments arranged by size: 3171 1500 1156 ApoI r'AATT_y Cuts at: 1493 2234 3136 3362 3514 5623 1493 Size: 741 902 226 152 2109 1697 Fragments arranged by size: 2109 1697 902 741 226 152 AseI AT'TA_AT Cuts at: 160 1032 4381 160 Size: 872 3349 1606 Fragments arranged by size: 3349 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5827 AvaI C'yCGr_G Cuts at: 1870 1943 2305 2489 2555 2921 3152 1870 Size: 73 362 184 66 366 231 4545 Fragments arranged by size: 4545 366 362 231 184 73 66 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2503 2536 2503 Size: 33 5794 BamHI G'GATC_C Cuts at: 3157 3524 3157 Size: 367 5460 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2641 3148 4603 618 Size: 325 217 627 854 507 1455 1842 Fragments arranged by size: 1842 1455 854 627 507 325 217 BanII G_rGCy'C Cuts at: 1391 2496 2784 3146 1391 Size: 1105 288 362 4072 Fragments arranged by size: 4072 1105 362 288 BbeI G_GCGC'C Cuts at: 2645 2645 Size: 5827 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4856 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2922 2956 4018 4052 1903 Size: 34 985 34 1062 34 3678 Fragments arranged by size: 3678 1062 985 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3719 4929 3719 Size: 1210 4617 BclI T'GATC_A Cuts at: 1548 1548 Size: 5827 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2942 4438 136 Size: 107 122 71 631 605 1270 1496 1525 Fragments arranged by size: 1525 1496 1270 631 605 122 107 71 BglII A'GATC_T Cuts at: 5822 5822 Size: 5827 BlpI GC'TnA_GC Cuts at: 3244 3244 Size: 5827 Bme1580I G_kGCm'C Cuts at: 1790 2305 2388 2690 3888 5044 1790 Size: 515 83 302 1198 1156 2573 Fragments arranged by size: 2573 1198 1156 515 302 83 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5827 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2870 3558 4516 Size: 416 53 83 399 24 1453 688 958 Cuts at: 4516 442 Size: 1753 Fragments arranged by size: 1753 1453 958 688 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5827 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2037 2348 2396 2585 2792 2798 2984 3127 Size: 798 311 48 189 207 6 186 143 Cuts at: 3127 4487 1239 Size: 1360 2579 Fragments arranged by size: 2579 1360 798 311 207 189 186 143 48 6 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3065 3952 4817 5115 3065 Size: 887 865 298 3777 Fragments arranged by size: 3777 887 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2067 4490 882 Size: 617 568 2423 2219 Fragments arranged by size: 2423 2219 617 568 BsaAI yAC'GTr Cuts at: 493 1204 3618 493 Size: 711 2414 2702 Fragments arranged by size: 2702 2414 711 BsaBI GATnn'nnATC Cuts at: 5821 5821 Size: 5827 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2370 2642 3039 3634 Size: 53 83 186 936 837 272 397 595 Cuts at: 3634 4016 4651 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 837 635 595 397 382 272 186 83 53 BsaWI w'CCGG_w Cuts at: 4260 4658 4932 5079 5490 4260 Size: 398 274 147 411 4597 Fragments arranged by size: 4597 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5602 Fragments arranged by size: 5602 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2048 2981 1186 Size: 862 933 4032 Fragments arranged by size: 4032 933 862 BseYI C'CCAG_C Cuts at: 5030 5526 5030 Size: 496 5331 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2582 1163 Size: 573 846 4408 Fragments arranged by size: 4408 846 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2946 3277 4038 5066 1540 Size: 275 186 945 331 761 1028 2301 Fragments arranged by size: 2301 1028 945 761 331 275 186 BsiHKAI G_wGCw'C Cuts at: 2388 2496 2556 3888 3973 5044 2388 Size: 108 60 1332 85 1071 3171 Fragments arranged by size: 3171 1332 1071 108 85 60 BsmI GAATG_Cn' Cuts at: 3338 3431 3338 Size: 93 5734 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2305 2388 2496 2556 2690 2784 3146 Size: 399 515 83 108 60 134 94 362 Cuts at: 3146 3888 3973 5044 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 515 399 362 134 108 94 85 83 60 BspEI T'CCGG_A Cuts at: 5490 5490 Size: 5827 BspHI T'CATG_A Cuts at: 3716 5446 3716 Size: 1730 4097 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2132 2436 3182 844 Size: 1288 304 746 3489 Fragments arranged by size: 3489 1288 746 304 BsrBI CCG'CTC Cuts at: 1394 1823 2350 3714 1394 Size: 429 527 1364 3507 Fragments arranged by size: 3507 1364 527 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4322 4496 65 Size: 1183 3074 174 1396 Fragments arranged by size: 3074 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4471 4658 1700 Size: 110 2661 187 2869 Fragments arranged by size: 2869 2661 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5827 BssHII G'CGCG_C Cuts at: 1925 2477 2584 2916 2929 2978 3048 1925 Size: 552 107 332 13 49 70 4704 Fragments arranged by size: 4704 552 332 107 70 49 13 BssSI C'ACGA_G Cuts at: 3119 3887 4899 3119 Size: 768 1012 4047 Fragments arranged by size: 4047 1012 768 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5827 BstBI TT'CG_AA Cuts at: 4631 4631 Size: 5827 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5827 BstYI r'GATC_y Cuts at: 1972 3157 3524 3909 3926 5378 5464 5476 5822 Size: 1185 367 385 17 1452 86 12 346 Cuts at: 5822 1972 Size: 1977 Fragments arranged by size: 1977 1452 1185 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2335 2832 513 Size: 552 939 331 497 3508 Fragments arranged by size: 3508 939 552 497 331 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5088 Fragments arranged by size: 5088 683 56 BtsI GCAGTG_nn' Cuts at: 2769 3052 3343 4137 4157 2769 Size: 283 291 794 20 4439 Fragments arranged by size: 4439 794 291 283 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5827 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5792 DraI TTT'AAA Cuts at: 3131 3131 Size: 5827 DraIII CAC_nnn'GTG Cuts at: 3031 3031 Size: 5827 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4834 809 Size: 507 3518 1802 Fragments arranged by size: 3518 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2268 2403 Size: 54 1425 50 275 18 177 261 135 Cuts at: 2403 2943 3274 4163 5792 8 Size: 540 331 889 1629 43 Fragments arranged by size: 1629 1425 889 540 331 275 261 177 135 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2943 3274 1537 Size: 275 1131 331 4090 Fragments arranged by size: 4090 1131 331 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2357 3757 1353 Size: 1004 1400 3423 Fragments arranged by size: 3423 1400 1004 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2045 2188 4414 4788 4934 387 Size: 1064 138 456 143 2226 374 146 1280 Fragments arranged by size: 2226 1280 1064 456 374 146 143 138 EcoICRI GAG'CTC Cuts at: 2494 2494 Size: 5827 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2037 2348 2396 2585 2792 2798 2984 Size: 393 405 311 48 189 207 6 186 Cuts at: 2984 3127 3890 4487 5274 5542 1239 Size: 143 763 597 787 268 1524 Fragments arranged by size: 1524 787 763 597 405 393 311 268 207 189 186 143 48 6 EcoNI CCTnn'n_nnAGG Cuts at: 2996 2996 Size: 5827 EcoO109I rG'GnC_Cy Cuts at: 2103 2540 2103 Size: 437 5390 EcoRI G'AATT_C Cuts at: 3136 3514 3136 Size: 378 5449 FspI TGC'GCA Cuts at: 3068 4333 3068 Size: 1265 4562 HaeII r_GCGC'y Cuts at: 1678 2133 2437 2645 2657 4974 1678 Size: 455 304 208 12 2317 2531 Fragments arranged by size: 2531 2317 455 304 208 12 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4468 4500 4542 4574 1373 Size: 32 135 32 2896 32 42 32 2626 Fragments arranged by size: 2896 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3171 3417 669 Size: 988 281 1233 246 3079 Fragments arranged by size: 3079 1233 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5827 HpaI GTT'AAC Cuts at: 3417 3417 Size: 5827 KasI G'GCGC_C Cuts at: 2641 2641 Size: 5827 KpnI G_GTAC'C Cuts at: 3152 3152 Size: 5827 MfeI C'AATT_G Cuts at: 3426 3426 Size: 5827 MluI A'CGCG_T Cuts at: 5703 5703 Size: 5827 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4941 5125 1305 Size: 3636 184 2007 Fragments arranged by size: 3636 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4005 Fragments arranged by size: 4005 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3785 4144 4303 518 Size: 648 2619 359 159 2042 Fragments arranged by size: 2619 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2117 2337 2484 2713 2795 3243 3920 5068 Size: 511 220 147 229 82 448 677 1148 Cuts at: 5068 5313 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 511 448 245 229 220 147 82 NarI GG'CG_CC Cuts at: 2642 2642 Size: 5827 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4336 Fragments arranged by size: 4336 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5827 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5827 NotI GC'GGCC_GC Cuts at: 3274 3274 Size: 5827 NspI r_CATG'y Cuts at: 1132 1229 2182 3185 4730 1132 Size: 97 953 1003 1545 2229 Fragments arranged by size: 2229 1545 1003 953 97 PasI CC'CwG_GG Cuts at: 1348 2753 1348 Size: 1405 4422 PciI A'CATG_T Cuts at: 4726 4726 Size: 5827 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5827 PfoI T'CCnGG_A Cuts at: 2373 2373 Size: 5827 PmeI GTTT'AAAC Cuts at: 3131 3131 Size: 5827 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3896 3928 5409 5441 3896 Size: 32 1481 32 4282 Fragments arranged by size: 4282 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2540 2540 Size: 5827 PshAI GACnn'nnGTC Cuts at: 1613 2810 1613 Size: 1197 4630 PsiI TTA'TAA Cuts at: 3397 3397 Size: 5827 PspXI vC'TCGA_Gb Cuts at: 2555 2921 2555 Size: 366 5461 PstI C_TGCA'G Cuts at: 830 2102 2129 2472 2820 3179 830 Size: 1272 27 343 348 359 3478 Fragments arranged by size: 3478 1272 359 348 343 27 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5827 PvuII CAG'CTG Cuts at: 2484 2484 Size: 5827 SacI G_AGCT'C Cuts at: 2496 2496 Size: 5827 SacII CC_GC'GG Cuts at: 2338 2338 Size: 5827 SalI G'TCGA_C Cuts at: 1936 3169 1936 Size: 1233 4594 SanDI GG'GwC_CC Cuts at: 2540 2540 Size: 5827 SapI GCTCTTCn'nnn_ Cuts at: 2357 2357 Size: 5827 SbfI CC_TGCA'GG Cuts at: 3179 3179 Size: 5827 ScaI AGT'ACT Cuts at: 4075 5787 4075 Size: 1712 4115 SfcI C'TryA_G Cuts at: 826 1045 2098 2125 2468 2816 3175 4310 4991 Size: 219 1053 27 343 348 359 1135 681 Cuts at: 4991 5182 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1053 681 359 348 343 219 191 27 SfoI GGC'GCC Cuts at: 2643 2643 Size: 5827 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5827 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5827 SmaI CCC'GGG Cuts at: 1872 2307 3154 1872 Size: 435 847 4545 Fragments arranged by size: 4545 847 435 SmlI C'TyrA_G Cuts at: 820 1017 1943 2555 2921 3080 3931 4832 5094 Size: 197 926 612 366 159 851 901 262 Cuts at: 5094 820 Size: 1553 Fragments arranged by size: 1553 926 901 851 612 366 262 197 159 SnaBI TAC'GTA Cuts at: 493 3618 493 Size: 3125 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5827 SphI G_CATG'C Cuts at: 1132 2182 3185 1132 Size: 1050 1003 3774 Fragments arranged by size: 3774 1050 1003 SspI AAT'ATT Cuts at: 5 52 3751 5 Size: 47 3699 2081 Fragments arranged by size: 3699 2081 47 StuI AGG'CCT Cuts at: 1517 2319 2570 3112 1517 Size: 802 251 542 4232 Fragments arranged by size: 4232 802 542 251 StyI C'CwwG_G Cuts at: 513 1065 2004 3266 513 Size: 552 939 1262 3074 Fragments arranged by size: 3074 1262 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2664 3854 3871 4024 4209 4654 325 Size: 1255 1084 1190 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1190 1084 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4073 5785 96 Size: 274 80 33 51 1443 2096 1712 138 Fragments arranged by size: 2096 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2037 2069 5787 5819 2037 Size: 32 3718 32 2045 Fragments arranged by size: 3718 2045 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5827 XbaI T'CTAG_A Cuts at: 3163 3163 Size: 5827 XhoI C'TCGA_G Cuts at: 1943 2555 2921 1943 Size: 612 366 4849 Fragments arranged by size: 4849 612 366 XmaI C'CCGG_G Cuts at: 1870 2305 3152 1870 Size: 435 847 4545 Fragments arranged by size: 4545 847 435 XmnI GAAnn'nnTTC Cuts at: 3956 3956 Size: 5827 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2371 3635 276 Size: 53 83 186 936 837 1264 2468 Fragments arranged by size: 2468 1264 936 837 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PsrI RsrII SexAI SfiI SrfI SwaI XcmI