################################## # NO CUT ENZYMES ( 155 enzymes) # ################################## AatII AccI AccIII AclI AcyI AflII AflIII AgeI AhdI AlwI AocI ApaI AscI AseI AsnI AspEI AspI AvaI AviII AvrII BalI BamHI BbeI BbrPI BbvCI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsoBI BspDI BspEI BspHI BspLU11I BsrBI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I CelII ClaI CpoI DpnII DraI DraII DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeIII HgaI Hin1I HincII HindII HindIII HpaI KasI Ksp632I KspI MaeI MaeII MamI MboI MflI MluI MluNI MroI MscI NaeI NarI NcoI NdeI NdeII NgoMIV NheI NotI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI Sau3AI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI SwaI TaqI Tth111I TthHB8I Van91I XbaI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### Acc65I 331 GGTACC G^GTACC AccII 607 CGCG CG^CG AcsI 441 GAATTT [GA]^AATT[CT] AlwNI 357 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 441 GAATTT [GA]^AATT[CT] Asp700I 630 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 331 GGTACC G^GTACC BanI 331 GGTACC G^G[CT][GA]CC BanII 72 GAGCCC G[GA]GC[CT]^C BbsI 315 GAAGAC GAAGAC(2/6) BcgI 609 CGATGACTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 528 CCGGG CC^[GC]GG BpuAI 315 GAAGAC GAAGAC(2/6) BsgI 210 GTGCAG GTGCAG(16/14) BsmFI 800 GGGAC GGGAC(10/14) BsmI 307 GAATGC GAATGC(1/-1) Bsp1407I 265 TGTACA T^GTACA BsrDI 25 CATTGC GCAATG(2/0) BsrFI 173 ACCGGC [GA]^CCGG[CT] BsrGI 265 TGTACA T^GTACA BstAPI 563 GCACCGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 607 CGCG CG^CG Cfr10I 173 ACCGGC [GA]^CCGG[CT] DdeI 5 CTGAG C^T[ACGT]AG DsaI 509 CCACGG C^C[GA][CT]GG EcoNI 60 CCTGCGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 245 CCAAGG C^C[AT][AT]GG HinfI 34 GAATC G^A[ACGT]TC HphI 506 TCACC GGTGA(8/7) KpnI 331 GGTACC GGTAC^C MboII 315 GAAGA GAAGA(8/7) MfeI 404 CAATTG C^AATTG MseI 746 TTAA T^TAA MunI 404 CAATTG C^AATTG MvnI 607 CGCG CG^CG NciI 528 CCGGG CC^[GC]GG NruI 606 TCGCGA TCG^CGA NspI 471 ACATGC [GA]CATG^[CT] PstI 570 CTGCAG CTGCA^G SbfI 569 CCTGCAGG CCTGCA^GG ScaI 287 AGTACT AGT^ACT SfcI 570 CTGCAG C^T[GA][CT]AG Sse8387I 569 CCTGCAGG CCTGCA^GG SspBI 265 TGTACA T^GTACA StyI 245 CCAAGG C^C[AT][AT]GG TfiI 34 GAATC G^A[AT]TC Tru9I 746 TTAA T^TAA XcmI 396 CCAGCAGCCAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 630 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### Alw44I 561 GTGCAC G^TGCAC 669 GTGCAC Aor51HI 8 AGCGCT AGC^GCT 593 AGCGCT ApaLI 561 GTGCAC G^TGCAC 669 GTGCAC AvaII 171 GGACC G^G[AT]CC 252 GGACC BsaI 97 GAGACC GGTCTC(1/5) 370 GAGACC BseRI 67 GAGGAG GAGGAG(10/8) 121 GAGGAG BsmAI 97 GAGAC GTCTC(1/5) 370 GAGAC BspMI 115 ACCTGC ACCTGC(4/8) 373 ACCTGC CfoI 9 GCGC GCG^C 594 GCGC Cfr13I 171 GGACC G^G[ACGT]CC 252 GGACC Eco47III 8 AGCGCT AGC^GCT 593 AGCGCT FauI 335 CCCGC CCCGC(4/6) 63 GCGGG HaeII 8 AGCGCT [GA]GCGC^[CT] 593 AGCGCT HapII 174 CCGG C^CGG 528 CCGG HhaI 9 GCGC GCG^C 594 GCGC HinP1I 9 GCGC G^CGC 594 GCGC HpaII 174 CCGG C^CGG 528 CCGG MslI 178 CACTGGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 293 CATCCAAGTG MspI 174 CCGG C^CGG 528 CCGG NlaIV 331 GGTACC GG[ACGT]^[ACGT]CC 409 GGAACC Sau96I 171 GGACC G^G[ACGT]CC 252 GGACC Tsp509I 405 AATT ^AATT 442 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 32 enzymes) # ######################################### AciI AfaI AluI AspHI BbvI BmyI BsaJI BsiHKAI BsiYI BslI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I EcoRII Fnu4HI FokI ItaI MaeIII MnlI MspA1I MvaI MwoI NlaIII PvuII RsaI ScrFI TseI Tsp45I TspRI