(Circular) (Six-base) MAPSORT of: pFN21AB6530.seq Check: 4277 from: 1 to: 5542 pFN21AB6530 5542 bp With 182 enzymes: SgfI * August 16, 2010 14:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2129 2129 Size: 5542 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3352 278 Size: 53 83 186 936 1816 2468 Fragments arranged by size: 2468 1816 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2885 1937 Size: 948 4594 Acc65I G'GTAC_C Cuts at: 2336 2863 2336 Size: 527 5015 AclI AA'CG_TT Cuts at: 1563 3669 4042 1563 Size: 2106 373 3063 Fragments arranged by size: 3063 2106 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3605 4989 5257 1632 Size: 1973 1384 268 1917 Fragments arranged by size: 1973 1917 1384 268 AfeI AGC'GCT Cuts at: 2015 2600 2015 Size: 585 4957 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5345 AflIII A'CryG_T Cuts at: 1203 4441 5418 1203 Size: 3238 977 1327 Fragments arranged by size: 3238 1327 977 AgeI A'CCGG_T Cuts at: 4373 4373 Size: 5542 AhdI GACnn_n'nnGTC Cuts at: 4271 4271 Size: 5542 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5542 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5542 AlwNI CAG_nnn'CTG Cuts at: 2367 3285 4857 2367 Size: 918 1572 3052 Fragments arranged by size: 3052 1572 918 ApaLI G'TGCA_C Cuts at: 2566 2674 3599 4755 2566 Size: 108 925 1156 3353 Fragments arranged by size: 3353 1156 925 108 ApoI r'AATT_y Cuts at: 1493 2446 2851 3077 3229 5338 1493 Size: 953 405 226 152 2109 1697 Fragments arranged by size: 2109 1697 953 405 226 152 AseI AT'TA_AT Cuts at: 160 1032 4096 160 Size: 872 3064 1606 Fragments arranged by size: 3064 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5542 AvaI C'yCGr_G Cuts at: 1870 1943 2867 1870 Size: 73 924 4545 Fragments arranged by size: 4545 924 73 BamHI G'GATC_C Cuts at: 2872 3239 2872 Size: 367 5175 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2336 2863 4318 618 Size: 325 217 627 549 527 1455 1842 Fragments arranged by size: 1842 1455 627 549 527 325 217 BanII G_rGCy'C Cuts at: 1391 2081 2861 1391 Size: 690 780 4072 Fragments arranged by size: 4072 780 690 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2327 928 Size: 971 428 4143 Fragments arranged by size: 4143 971 428 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2603 2637 3733 3767 1903 Size: 34 666 34 1096 34 3678 Fragments arranged by size: 3678 1096 666 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3434 4644 3434 Size: 1210 4332 BclI T'GATC_A Cuts at: 1548 1548 Size: 5542 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4153 136 Size: 107 122 71 631 605 2481 1525 Fragments arranged by size: 2481 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5537 5537 Size: 5542 BlpI GC'TnA_GC Cuts at: 2959 2959 Size: 5542 Bme1580I G_kGCm'C Cuts at: 1790 2460 2570 2678 2822 3603 4759 1790 Size: 670 110 108 144 781 1156 2573 Fragments arranged by size: 2573 1156 781 670 144 110 108 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5542 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3273 4231 442 Size: 416 53 83 399 24 1856 958 1753 Fragments arranged by size: 1856 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5542 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4202 1239 Size: 2963 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3667 4532 4830 3667 Size: 865 298 4379 Fragments arranged by size: 4379 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2096 2369 4205 882 Size: 617 597 273 1836 2219 Fragments arranged by size: 2219 1836 617 597 273 BsaAI yAC'GTr Cuts at: 493 1204 3333 493 Size: 711 2129 2702 Fragments arranged by size: 2702 2129 711 BsaBI GATnn'nnATC Cuts at: 5536 5536 Size: 5542 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3349 3731 4366 275 Size: 53 83 186 936 1816 382 635 1451 Fragments arranged by size: 1816 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3975 4373 4647 4794 5205 3975 Size: 398 274 147 411 4312 Fragments arranged by size: 4312 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5317 Fragments arranged by size: 5317 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2087 2141 1186 Size: 901 54 4587 Fragments arranged by size: 4587 901 54 BseYI C'CCAG_C Cuts at: 2669 4745 5241 2669 Size: 2076 496 2970 Fragments arranged by size: 2970 2076 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2236 1163 Size: 573 500 4469 Fragments arranged by size: 4469 573 500 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2992 3753 4781 1540 Size: 275 186 991 761 1028 2301 Fragments arranged by size: 2301 1028 991 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2570 2678 2768 3603 3688 4759 2570 Size: 108 90 835 85 1071 3353 Fragments arranged by size: 3353 1071 835 108 90 85 BsmI GAATG_Cn' Cuts at: 2318 3053 3146 2318 Size: 735 93 4714 Fragments arranged by size: 4714 735 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2081 2460 2570 2678 2768 2822 2861 Size: 399 291 379 110 108 90 54 39 Cuts at: 2861 3603 3688 4759 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 379 291 110 108 90 85 54 39 BspEI T'CCGG_A Cuts at: 5205 5205 Size: 5542 BspHI T'CATG_A Cuts at: 3431 5161 3431 Size: 1730 3812 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2129 2387 2897 844 Size: 1285 258 510 3489 Fragments arranged by size: 3489 1285 510 258 BsrBI CCG'CTC Cuts at: 1394 1823 3429 1394 Size: 429 1606 3507 Fragments arranged by size: 3507 1606 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2029 4037 4211 65 Size: 1183 781 2008 174 1396 Fragments arranged by size: 2008 1396 1183 781 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2178 4186 4373 1700 Size: 110 368 2008 187 2869 Fragments arranged by size: 2869 2008 368 187 110 BsrGI T'GTAC_A Cuts at: 96 2270 96 Size: 2174 3368 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5542 BssSI C'ACGA_G Cuts at: 3602 4614 3602 Size: 1012 4530 BstAPI GCAn_nnn'nTGC Cuts at: 794 2574 794 Size: 1780 3762 BstBI TT'CG_AA Cuts at: 4346 4346 Size: 5542 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5542 BstYI r'GATC_y Cuts at: 1972 2872 3239 3624 3641 5093 5179 5191 5537 Size: 900 367 385 17 1452 86 12 346 Cuts at: 5537 1972 Size: 1977 Fragments arranged by size: 1977 1452 900 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2514 513 Size: 552 1449 3541 Fragments arranged by size: 3541 1449 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2628 487 Size: 683 56 1402 3401 Fragments arranged by size: 3401 1402 683 56 BtsI GCAGTG_nn' Cuts at: 2203 2219 2492 2738 3058 3852 3872 2203 Size: 16 273 246 320 794 20 3873 Fragments arranged by size: 3873 794 320 273 246 20 16 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5542 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5507 DraI TTT'AAA Cuts at: 2846 2846 Size: 5542 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4549 809 Size: 507 3233 1802 Fragments arranged by size: 3233 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2989 3878 5507 Size: 54 1425 50 275 18 1159 889 1629 Cuts at: 5507 8 Size: 43 Fragments arranged by size: 1629 1425 1159 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2989 1537 Size: 275 1177 4090 Fragments arranged by size: 4090 1177 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3472 1353 Size: 2119 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4129 4503 4649 387 Size: 1064 138 2540 374 146 1280 Fragments arranged by size: 2540 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3605 4202 4989 5257 1239 Size: 393 1973 597 787 268 1524 Fragments arranged by size: 1973 1524 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2069 2069 Size: 5542 EcoRI G'AATT_C Cuts at: 2851 3229 2851 Size: 378 5164 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2575 2575 Size: 5542 FspI TGC'GCA Cuts at: 4048 4048 Size: 5542 HaeII r_GCGC'y Cuts at: 1678 2017 2602 4689 1678 Size: 339 585 2087 2531 Fragments arranged by size: 2531 2087 585 339 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2282 2314 4183 4215 4257 Size: 32 135 32 710 32 1869 32 42 Cuts at: 4257 4289 1373 Size: 32 2626 Fragments arranged by size: 2626 1869 710 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2886 3132 669 Size: 988 281 948 246 3079 Fragments arranged by size: 3079 988 948 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5542 HpaI GTT'AAC Cuts at: 3132 3132 Size: 5542 KpnI G_GTAC'C Cuts at: 2340 2867 2340 Size: 527 5015 MfeI C'AATT_G Cuts at: 2409 3141 2409 Size: 732 4810 MluI A'CGCG_T Cuts at: 5418 5418 Size: 5542 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2310 4656 4840 1305 Size: 1005 2346 184 2007 Fragments arranged by size: 2346 2007 1005 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3720 Fragments arranged by size: 3720 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2187 2302 3500 3859 4018 518 Size: 648 1021 115 1198 359 159 2042 Fragments arranged by size: 2042 1198 1021 648 359 159 115 MspA1I CmG'CkG Cuts at: 1606 2263 2367 2397 2587 2691 2958 3635 4783 Size: 657 104 30 190 104 267 677 1148 Cuts at: 4783 5028 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 657 267 245 190 104 104 30 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4990 NdeI CA'TA_TG Cuts at: 387 387 Size: 5542 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5542 NotI GC'GGCC_GC Cuts at: 2989 2989 Size: 5542 NruI TCG'CGA Cuts at: 2613 2613 Size: 5542 NspI r_CATG'y Cuts at: 1132 1229 2480 2900 4445 1132 Size: 97 1251 420 1545 2229 Fragments arranged by size: 2229 1545 1251 420 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5542 PciI A'CATG_T Cuts at: 4441 4441 Size: 5542 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5542 PmeI GTTT'AAAC Cuts at: 2846 2846 Size: 5542 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2093 2125 3611 3643 5124 5156 2093 Size: 32 1486 32 1481 32 2479 Fragments arranged by size: 2479 1486 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5542 PsiI TTA'TAA Cuts at: 3112 3112 Size: 5542 PstI C_TGCA'G Cuts at: 830 2579 2894 830 Size: 1749 315 3478 Fragments arranged by size: 3478 1749 315 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5542 PvuII CAG'CTG Cuts at: 2263 2367 2587 2263 Size: 104 220 5218 Fragments arranged by size: 5218 220 104 SalI G'TCGA_C Cuts at: 1936 2884 1936 Size: 948 4594 SbfI CC_TGCA'GG Cuts at: 2579 2894 2579 Size: 315 5227 ScaI AGT'ACT Cuts at: 2294 3790 5502 2294 Size: 1496 1712 2334 Fragments arranged by size: 2334 1712 1496 SfcI C'TryA_G Cuts at: 826 1045 2575 2890 4025 4706 4897 826 Size: 219 1530 315 1135 681 191 1471 Fragments arranged by size: 1530 1471 1135 681 315 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5542 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5542 SmaI CCC'GGG Cuts at: 1872 2869 1872 Size: 997 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3646 4547 4809 820 Size: 197 926 1703 901 262 1553 Fragments arranged by size: 1703 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3333 493 Size: 2840 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5542 SphI G_CATG'C Cuts at: 1132 2900 1132 Size: 1768 3774 SspI AAT'ATT Cuts at: 5 52 3466 5 Size: 47 3414 2081 Fragments arranged by size: 3414 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5542 StyI C'CwwG_G Cuts at: 513 1065 2250 2981 513 Size: 552 1185 731 3074 Fragments arranged by size: 3074 1185 731 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2661 3569 3586 3739 3924 4369 325 Size: 1255 1081 908 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1081 908 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2058 2270 2292 Size: 274 80 33 51 1443 81 212 22 Cuts at: 2292 2352 3788 5500 96 Size: 60 1436 1712 138 Fragments arranged by size: 1712 1443 1436 274 212 138 81 80 60 51 33 22 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2717 2749 5502 5534 2717 Size: 32 2753 32 2725 Fragments arranged by size: 2753 2725 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5542 XbaI T'CTAG_A Cuts at: 2878 2878 Size: 5542 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2408 2408 Size: 5542 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5542 XmaI C'CCGG_G Cuts at: 1870 2867 1870 Size: 997 4545 XmnI GAAnn'nnTTC Cuts at: 2639 3671 2639 Size: 1032 4510 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3350 276 Size: 53 83 186 936 1816 2468 Fragments arranged by size: 2468 1816 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI