################################## # NO CUT ENZYMES ( 112 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AhdI AocI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbrPI BbvCI BcgI BciVI BclI BfrI BglII BlnI BsaAI BsaBI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspLU11I BsrDI BsrGI BssHII Bst1107I BstAPI BstBI BstYI BstZ17I Bsu36I ClaI CpoI DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KpnI Ksp632I MamI MfeI MflI MluI MroI MunI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PflFI PflMI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RsrII SapI SbfI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SwaI Tth111I Van91I XbaI XhoII XmnI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### AccI 643 GTCGAC GT^[AC][GT]AC AcsI 685 GAATTT [GA]^AATT[CT] AfaI 285 GTAC GT^AC AflIII 53 ACGTGT A^C[GA][CT]GT AgeI 500 ACCGGT A^CCGGT Alw44I 762 GTGCAC G^TGCAC AlwI 618 GATCC GGATC(4/5) ApaLI 762 GTGCAC G^TGCAC ApoI 685 GAATTT [GA]^AATT[CT] BbeI 136 GGCGCC GGCGC^C BfaI 718 CTAG C^TAG BglI 255 GCCCTCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 607 GCTGAGC GC^T[ACGT]AGC Bpu1102I 607 GCTGAGC GC^T[ACGT]AGC BsaI 878 GAGACC GGTCTC(1/5) BsaWI 500 ACCGGT [AT]^CCGG[AT] BseRI 490 CTCCTC GAGGAG(10/8) BspMI 743 ACCTGC ACCTGC(4/8) BsrFI 500 ACCGGT [GA]^CCGG[CT] BssSI 895 CTCGTG CACGAG(-5/-1) BstEII 84 GGTGACC G^GT[ACGT]ACC BstPI 84 GGTGACC G^GT[ACGT]ACC BstXI 5 CCAGTGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 607 GCTGAGC GC^T[ACGT]AGC Cfr10I 500 ACCGGT [GA]^CCGG[CT] DpnII 618 GATC ^GATC DraI 421 TTTAAA TTT^AAA EcoO65I 84 GGTGACC G^GT[ACGT]ACC HaeII 136 GGCGCC [GA]GCGC^[CT] HincII 643 GTCGAC GT[CT]^[GA]AC HindII 643 GTCGAC GT[CT]^[GA]AC KasI 136 GGCGCC G^GCGCC KspI 1041 CCGCGG CCGC^GG MaeI 718 CTAG C^TAG MboI 618 GATC ^GATC MseI 422 TTAA T^TAA NarI 136 GGCGCC GG^CGCC NcoI 167 CCATGG C^CATGG NdeII 618 GATC ^GATC PaeR7I 196 CTCGAG C^TCGAG PinAI 500 ACCGGT A^CCGGT PleI 646 GACTC GAGTC(4/5) PpuMI 731 AGGACCT [GA]G^G[AT]CC[CT] RsaI 285 GTAC GT^AC SacII 1041 CCGCGG CCGC^GG SalI 643 GTCGAC G^TCGAC Sau3AI 618 GATC ^GATC SfaNI 383 GCATC GCATC(5/9) SfoI 136 GGCGCC GGC^GCC SmaI 827 CCCGGG CCC^GGG SphI 210 GCATGC GCATG^C SspI 472 AATATT AAT^ATT StuI 302 AGGCCT AGG^CCT Tru9I 422 TTAA T^TAA Tsp509I 686 AATT ^AATT XhoI 196 CTCGAG C^TCGAG XmaCI 827 CCCGGG C^CCGGG XmaI 827 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AccII 93 CGCG CG^CG 1042 CGCG AcyI 136 GGCGCC G[GA]^CG[CT]C 787 GGCGTC AlwNI 574 CAGTATCTG CAG[ACGT][ACGT][ACGT]^CTG 974 CAGCGTCTG BalI 506 TGGCCA TGG^CCA 934 TGGCCA BpmI 691 CTCCAG CTGGAG(16/14) 124 CTGGAG BsaHI 136 GGCGCC G[GA]^CG[CT]C 787 GGCGTC BsgI 229 CTGCAC GTGCAG(16/14) 745 CTGCAC BspHI 614 TCATGA T^CATGA 1065 TCATGA BsrBI 921 CCGCTC CCGCTC(-3/-3) 199 GAGCGG BstF5I 360 GGATG GGATG(2/0) 657 GGATG BstUI 93 CGCG CG^CG 1042 CGCG Cac8I 210 GCATGC GC[ACGT]^[ACGT]GC 264 GCAGGC DraII 731 AGGACCT [GA]G^G[ACGT]CC[CT] 1010 AGGCCCT EcoO109I 731 AGGACCT [GA]G^G[ACGT]CC[CT] 1010 AGGCCCT EcoT14I 167 CCATGG C^C[AT][AT]GG 633 CCTTGG FauI 105 GCGGG CCCGC(4/6) 453 GCGGG FokI 360 GGATG GGATG(9/13) 657 GGATG Hin1I 136 GGCGCC G[GA]^CG[CT]C 787 GGCGTC MluNI 506 TGGCCA TGG^CCA 934 TGGCCA MscI 506 TGGCCA TGG^CCA 934 TGGCCA MslI 18 CATCTTCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 663 CATGGACATG MspA1I 146 CAGCGG C[AC]G^C[GT]G 1041 CCGCGG MvnI 93 CGCG CG^CG 1042 CGCG NspI 191 ACATGC [GA]CATG^[CT] 210 GCATGC PstI 154 CTGCAG CTGCA^G 571 CTGCAG RcaI 614 TCATGA T^CATGA 1065 TCATGA SacI 610 GAGCTC GAGCT^C 706 GAGCTC SfcI 154 CTGCAG C^T[GA][CT]AG 571 CTGCAG StyI 167 CCATGG C^C[AT][AT]GG 633 CCTTGG Tsp45I 85 GTGAC ^GT[GC]AC 590 GTGAC XcmI 347 CCAAGAAAGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 984 CCACTCCCTCGATGG ######################################### # 3 OR MORE CUT ENZYMES ( 57 enzymes) # ######################################### AciI AluI AspHI AvaI AvaII BanI BanII BbsI BbvI BcnI BmyI BpuAI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstNI CfoI Cfr13I DdeI DsaI EaeI EcoRII Fnu4HI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboII MnlI MspI MvaI MwoI NciI NlaIII NlaIV Sau96I ScrFI SmlI TaqI TfiI TseI TspRI TthHB8I