(Circular) (Six-base) MAPSORT of: pFN21AE5522.seq Check: 6439 from: 1 to: 5776 pFN21AE5522 5776 bp With 182 enzymes: SgfI * January 30, 2014 17:22 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3586 278 Size: 53 83 186 936 2050 2468 Fragments arranged by size: 2468 2050 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2649 3119 1937 Size: 712 470 4594 Fragments arranged by size: 4594 712 470 Acc65I G'GTAC_C Cuts at: 3097 3097 Size: 5776 AclI AA'CG_TT Cuts at: 1563 3903 4276 1563 Size: 2340 373 3063 Fragments arranged by size: 3063 2340 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3839 5223 5491 1632 Size: 2207 1384 268 1917 Fragments arranged by size: 2207 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5579 AflIII A'CryG_T Cuts at: 1203 2058 4675 5652 1203 Size: 855 2617 977 1327 Fragments arranged by size: 2617 1327 977 855 AgeI A'CCGG_T Cuts at: 2505 4607 2505 Size: 2102 3674 AhdI GACnn_n'nnGTC Cuts at: 4505 4505 Size: 5776 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5776 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5776 AlwNI CAG_nnn'CTG Cuts at: 2584 2984 3519 5091 2584 Size: 400 535 1572 3269 Fragments arranged by size: 3269 1572 535 400 ApaLI G'TGCA_C Cuts at: 2767 3833 4989 2767 Size: 1066 1156 3554 Fragments arranged by size: 3554 1156 1066 ApoI r'AATT_y Cuts at: 1493 2690 3085 3311 3463 5572 1493 Size: 1197 395 226 152 2109 1697 Fragments arranged by size: 2109 1697 1197 395 226 152 AseI AT'TA_AT Cuts at: 160 1032 4330 160 Size: 872 3298 1606 Fragments arranged by size: 3298 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5776 AvaI C'yCGr_G Cuts at: 1870 1943 2119 2201 2832 3101 1870 Size: 73 176 82 631 269 4545 Fragments arranged by size: 4545 631 269 176 82 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2564 2597 2564 Size: 33 5743 BamHI G'GATC_C Cuts at: 3106 3473 3106 Size: 367 5409 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2141 2348 3038 3097 4552 Size: 325 217 627 354 207 690 59 1455 Cuts at: 4552 618 Size: 1842 Fragments arranged by size: 1842 1455 690 627 354 325 217 207 59 BanII G_rGCy'C Cuts at: 1391 2465 2619 2715 3095 1391 Size: 1074 154 96 380 4072 Fragments arranged by size: 4072 1074 380 154 96 BbeI G_GCGC'C Cuts at: 2145 2145 Size: 5776 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2428 2527 2788 928 Size: 971 529 99 261 3916 Fragments arranged by size: 3916 971 529 261 99 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3967 4001 1903 Size: 34 2030 34 3678 Fragments arranged by size: 3678 2030 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3668 4878 3668 Size: 1210 4566 BclI T'GATC_A Cuts at: 1548 1548 Size: 5776 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2266 4387 136 Size: 107 122 71 631 605 594 2121 1525 Fragments arranged by size: 2121 1525 631 605 594 122 107 71 BglII A'GATC_T Cuts at: 5771 5771 Size: 5776 BlpI GC'TnA_GC Cuts at: 2613 3193 2613 Size: 580 5196 Bme1580I G_kGCm'C Cuts at: 1790 2771 3041 3837 4993 1790 Size: 981 270 796 1156 2573 Fragments arranged by size: 2573 1156 981 796 270 BmgBI CAC'GTC Cuts at: 1318 2059 2953 1318 Size: 741 894 4141 Fragments arranged by size: 4141 894 741 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3507 4465 442 Size: 416 53 83 399 24 2090 958 1753 Fragments arranged by size: 2090 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5776 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2150 2681 4436 1239 Size: 911 531 1755 2579 Fragments arranged by size: 2579 1755 911 531 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2381 2699 3901 4766 5064 2381 Size: 318 1202 865 298 3093 Fragments arranged by size: 3093 1202 865 318 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2877 4439 882 Size: 617 1378 1562 2219 Fragments arranged by size: 2219 1562 1378 617 BsaAI yAC'GTr Cuts at: 493 1204 3567 493 Size: 711 2363 2702 Fragments arranged by size: 2702 2363 711 BsaBI GATnn'nnATC Cuts at: 5770 5770 Size: 5776 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2142 2793 3583 3965 Size: 53 83 186 936 609 651 790 382 Cuts at: 3965 4600 275 Size: 635 1451 Fragments arranged by size: 1451 936 790 651 635 609 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2505 4209 4607 4881 5028 5439 2505 Size: 1704 398 274 147 411 2842 Fragments arranged by size: 2842 1704 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2478 2508 1178 Size: 30 165 30 1075 30 4446 Fragments arranged by size: 4446 1075 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2486 1186 Size: 1300 4476 BseYI C'CCAG_C Cuts at: 2326 2788 4979 5475 2326 Size: 462 2191 496 2627 Fragments arranged by size: 2627 2191 496 462 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2219 2735 1163 Size: 573 483 516 4204 Fragments arranged by size: 4204 573 516 483 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3226 3987 5015 1540 Size: 275 186 1225 761 1028 2301 Fragments arranged by size: 2301 1225 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2619 2715 2771 3837 3922 4993 2619 Size: 96 56 1066 85 1071 3402 Fragments arranged by size: 3402 1071 1066 96 85 56 BsmI GAATG_Cn' Cuts at: 3287 3380 3287 Size: 93 5683 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2465 2619 2715 2771 3041 3095 3837 Size: 399 675 154 96 56 270 54 742 Cuts at: 3837 3922 4993 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 675 399 270 154 96 85 56 54 BspEI T'CCGG_A Cuts at: 5439 5439 Size: 5776 BspHI T'CATG_A Cuts at: 2619 3070 3665 5395 2619 Size: 451 595 1730 3000 Fragments arranged by size: 3000 1730 595 451 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2757 3131 844 Size: 1913 374 3489 Fragments arranged by size: 3489 1913 374 BsrBI CCG'CTC Cuts at: 1394 1823 2206 2928 3663 1394 Size: 429 383 722 735 3507 Fragments arranged by size: 3507 735 722 429 383 BsrDI GCAATG_nn' Cuts at: 65 1248 4271 4445 65 Size: 1183 3023 174 1396 Fragments arranged by size: 3023 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2505 4420 4607 1700 Size: 110 695 1915 187 2869 Fragments arranged by size: 2869 1915 695 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5776 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5776 BssSI C'ACGA_G Cuts at: 2900 3836 4848 2900 Size: 936 1012 3828 Fragments arranged by size: 3828 1012 936 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5776 BstBI TT'CG_AA Cuts at: 4580 4580 Size: 5776 BstEII G'GTnAC_C Cuts at: 1184 2089 1184 Size: 905 4871 BstXI CCAn_nnnn'nTGG Cuts at: 2017 2017 Size: 5776 BstYI r'GATC_y Cuts at: 1972 3106 3473 3858 3875 5327 5413 5425 5771 Size: 1134 367 385 17 1452 86 12 346 Cuts at: 5771 1972 Size: 1977 Fragments arranged by size: 1977 1452 1134 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2145 2172 3046 513 Size: 552 1080 27 874 3243 Fragments arranged by size: 3243 1080 874 552 27 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5037 Fragments arranged by size: 5037 683 56 BtsI GCAGTG_nn' Cuts at: 2769 3292 4086 4106 2769 Size: 523 794 20 4439 Fragments arranged by size: 4439 794 523 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5776 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5741 DraI TTT'AAA Cuts at: 2428 3080 2428 Size: 652 5124 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4783 809 Size: 507 3467 1802 Fragments arranged by size: 3467 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2511 2850 2939 Size: 54 1425 50 275 18 681 339 89 Cuts at: 2939 3223 4112 5741 8 Size: 284 889 1629 43 Fragments arranged by size: 1629 1425 889 681 339 284 275 89 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3223 1537 Size: 275 1411 4090 Fragments arranged by size: 4090 1411 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3706 1353 Size: 2353 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2247 4363 4737 4883 387 Size: 1064 138 658 2116 374 146 1280 Fragments arranged by size: 2116 1280 1064 658 374 146 138 EcoICRI GAG'CTC Cuts at: 2617 2713 2617 Size: 96 5680 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2150 2681 3839 4436 5223 5491 1239 Size: 393 518 531 1158 597 787 268 1524 Fragments arranged by size: 1524 1158 787 597 531 518 393 268 EcoO109I rG'GnC_Cy Cuts at: 2737 3016 2737 Size: 279 5497 EcoRI G'AATT_C Cuts at: 3085 3463 3085 Size: 378 5398 FspI TGC'GCA Cuts at: 4282 4282 Size: 5776 HaeII r_GCGC'y Cuts at: 1678 2145 4923 1678 Size: 467 2778 2531 Fragments arranged by size: 2778 2531 467 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2069 2101 4417 4449 4491 Size: 32 135 32 497 32 2316 32 42 Cuts at: 4491 4523 1373 Size: 32 2626 Fragments arranged by size: 2626 2316 497 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2650 3120 3366 669 Size: 988 281 712 470 246 3079 Fragments arranged by size: 3079 988 712 470 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5776 HpaI GTT'AAC Cuts at: 3366 3366 Size: 5776 KasI G'GCGC_C Cuts at: 2141 2141 Size: 5776 KpnI G_GTAC'C Cuts at: 3101 3101 Size: 5776 MfeI C'AATT_G Cuts at: 3375 3375 Size: 5776 MluI A'CGCG_T Cuts at: 5652 5652 Size: 5776 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2955 3031 4890 5074 1305 Size: 1650 76 1859 184 2007 Fragments arranged by size: 2007 1859 1650 184 76 MscI TGG'CCA Cuts at: 10 64 1489 1832 2513 2941 10 Size: 54 1425 343 681 428 2845 Fragments arranged by size: 2845 1425 681 428 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2027 2672 3734 4093 4252 518 Size: 648 861 645 1062 359 159 2042 Fragments arranged by size: 2042 1062 861 648 645 359 159 MspA1I CmG'CkG Cuts at: 1606 2153 3048 3192 3869 5017 5262 1606 Size: 547 895 144 677 1148 245 2120 Fragments arranged by size: 2120 1148 895 677 547 245 144 NarI GG'CG_CC Cuts at: 2142 2142 Size: 5776 NcoI C'CATG_G Cuts at: 513 1065 2172 513 Size: 552 1107 4117 Fragments arranged by size: 4117 1107 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5776 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5776 NotI GC'GGCC_GC Cuts at: 3223 3223 Size: 5776 NspI r_CATG'y Cuts at: 1132 1229 2200 2219 3134 4679 1132 Size: 97 971 19 915 1545 2229 Fragments arranged by size: 2229 1545 971 915 97 19 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5776 PciI A'CATG_T Cuts at: 4675 4675 Size: 5776 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5776 PfoI T'CCnGG_A Cuts at: 2127 2697 2127 Size: 570 5206 PmeI GTTT'AAAC Cuts at: 3080 3080 Size: 5776 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3845 3877 5358 5390 3845 Size: 32 1481 32 4231 Fragments arranged by size: 4231 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2737 2737 Size: 5776 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5776 PsiI TTA'TAA Cuts at: 3346 3346 Size: 5776 PspXI vC'TCGA_Gb Cuts at: 2201 2201 Size: 5776 PstI C_TGCA'G Cuts at: 830 2163 2580 3128 830 Size: 1333 417 548 3478 Fragments arranged by size: 3478 1333 548 417 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5776 SacI G_AGCT'C Cuts at: 2619 2715 2619 Size: 96 5680 SacII CC_GC'GG Cuts at: 3049 3049 Size: 5776 SalI G'TCGA_C Cuts at: 1936 2648 3118 1936 Size: 712 470 4594 Fragments arranged by size: 4594 712 470 SbfI CC_TGCA'GG Cuts at: 3128 3128 Size: 5776 ScaI AGT'ACT Cuts at: 4024 5736 4024 Size: 1712 4064 SfcI C'TryA_G Cuts at: 826 1045 2159 2576 3124 4259 4940 5131 826 Size: 219 1114 417 548 1135 681 191 1471 Fragments arranged by size: 1471 1135 1114 681 548 417 219 191 SfoI GGC'GCC Cuts at: 2143 2143 Size: 5776 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5776 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5776 SmaI CCC'GGG Cuts at: 1872 2834 3103 1872 Size: 962 269 4545 Fragments arranged by size: 4545 962 269 SmlI C'TyrA_G Cuts at: 820 1017 1943 2201 2396 2714 3880 4781 5043 Size: 197 926 258 195 318 1166 901 262 Cuts at: 5043 820 Size: 1553 Fragments arranged by size: 1553 1166 926 901 318 262 258 197 195 SnaBI TAC'GTA Cuts at: 493 3567 493 Size: 3074 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5776 SphI G_CATG'C Cuts at: 1132 2219 3134 1132 Size: 1087 915 3774 Fragments arranged by size: 3774 1087 915 SspI AAT'ATT Cuts at: 5 52 2479 3700 5 Size: 47 2427 1221 2081 Fragments arranged by size: 2427 2081 1221 47 StuI AGG'CCT Cuts at: 1517 2309 1517 Size: 792 4984 StyI C'CwwG_G Cuts at: 513 1065 2172 2638 3215 513 Size: 552 1107 466 577 3074 Fragments arranged by size: 3074 1107 577 552 466 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2108 3803 3820 3973 4158 4603 325 Size: 1255 528 1695 17 153 185 445 1498 Fragments arranged by size: 1695 1498 1255 528 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4022 5734 96 Size: 274 80 33 51 1443 2045 1712 138 Fragments arranged by size: 2045 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5736 5768 5736 Size: 32 5744 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5776 XbaI T'CTAG_A Cuts at: 3112 3112 Size: 5776 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2359 2996 2359 Size: 637 5139 XhoI C'TCGA_G Cuts at: 1943 2201 1943 Size: 258 5518 XmaI C'CCGG_G Cuts at: 1870 2832 3101 1870 Size: 962 269 4545 Fragments arranged by size: 4545 962 269 XmnI GAAnn'nnTTC Cuts at: 3905 3905 Size: 5776 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3584 276 Size: 53 83 186 936 2050 2468 Fragments arranged by size: 2468 2050 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI SacI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PsrI PvuII RsrII SanDI SapI SexAI SfiI SrfI SwaI