(Linear) (Six-base) MAPSORT of: KIEE5522.seq Check: 9246 from: 1 to: 1071 KIEE5522.orf With 182 enzymes: SgfI * January 29, 2014 12:04 .. AccI GT'mk_AC Cuts at: 0 644 1071 Size: 644 427 AflIII A'CryG_T Cuts at: 0 53 1071 Size: 53 1018 AgeI A'CCGG_T Cuts at: 0 500 1071 Size: 500 571 AlwNI CAG_nnn'CTG Cuts at: 0 579 979 1071 Size: 579 400 92 Fragments arranged by size: 579 400 92 ApaLI G'TGCA_C Cuts at: 0 762 1071 Size: 762 309 ApoI r'AATT_y Cuts at: 0 685 1071 Size: 685 386 AvaI C'yCGr_G Cuts at: 0 114 196 827 1071 Size: 114 82 631 244 Fragments arranged by size: 631 244 114 82 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 559 592 1071 Size: 559 33 479 Fragments arranged by size: 559 479 33 BanI G'GyrC_C Cuts at: 0 136 343 1033 1071 Size: 136 207 690 38 Fragments arranged by size: 690 207 136 38 BanII G_rGCy'C Cuts at: 0 460 614 710 1071 Size: 460 154 96 361 Fragments arranged by size: 460 361 154 96 BbeI G_GCGC'C Cuts at: 0 140 1071 Size: 140 931 BbsI GAAGACnn'nnnn_ Cuts at: 0 423 522 783 1071 Size: 423 99 261 288 Fragments arranged by size: 423 288 261 99 BglI GCCn_nnn'nGGC Cuts at: 0 261 1071 Size: 261 810 BlpI GC'TnA_GC Cuts at: 0 608 1071 Size: 608 463 Bme1580I G_kGCm'C Cuts at: 0 766 1036 1071 Size: 766 270 35 Fragments arranged by size: 766 270 35 BmgBI CAC'GTC Cuts at: 0 54 948 1071 Size: 54 894 123 Fragments arranged by size: 894 123 54 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 145 676 1071 Size: 145 531 395 Fragments arranged by size: 531 395 145 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 376 694 1071 Size: 376 318 377 Fragments arranged by size: 377 376 318 BsaI GGTCTCn'nnnn_ Cuts at: 0 872 1071 Size: 872 199 BsaHI Gr'CG_yC Cuts at: 0 137 788 1071 Size: 137 651 283 Fragments arranged by size: 651 283 137 BsaWI w'CCGG_w Cuts at: 0 500 1071 Size: 500 571 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 473 503 1071 Size: 473 30 568 Fragments arranged by size: 568 473 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 481 1071 Size: 481 590 BseYI C'CCAG_C Cuts at: 0 321 783 1071 Size: 321 462 288 Fragments arranged by size: 462 321 288 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 214 730 1071 Size: 214 516 341 Fragments arranged by size: 516 341 214 BsiHKAI G_wGCw'C Cuts at: 0 614 710 766 1071 Size: 614 96 56 305 Fragments arranged by size: 614 305 96 56 Bsp1286I G_dGCh'C Cuts at: 0 460 614 710 766 1036 1071 Size: 460 154 96 56 270 35 Fragments arranged by size: 460 270 154 96 56 35 BspHI T'CATG_A Cuts at: 0 614 1065 1071 Size: 614 451 6 Fragments arranged by size: 614 451 6 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 752 1071 Size: 752 319 BsrBI CCG'CTC Cuts at: 0 201 923 1071 Size: 201 722 148 Fragments arranged by size: 722 201 148 BsrFI r'CCGG_y Cuts at: 0 500 1071 Size: 500 571 BssSI C'ACGA_G Cuts at: 0 895 1071 Size: 895 176 BstEII G'GTnAC_C Cuts at: 0 84 1071 Size: 84 987 BstXI CCAn_nnnn'nTGG Cuts at: 0 12 1071 Size: 12 1059 BtgI C'CryG_G Cuts at: 0 140 167 1041 1071 Size: 140 27 874 30 Fragments arranged by size: 874 140 30 27 BtsI GCAGTG_nn' Cuts at: 0 764 1071 Size: 764 307 DraI TTT'AAA Cuts at: 0 423 1071 Size: 423 648 EaeI y'GGCC_r Cuts at: 0 506 845 934 1071 Size: 506 339 89 137 Fragments arranged by size: 506 339 137 89 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 242 1071 Size: 242 829 EcoICRI GAG'CTC Cuts at: 0 612 708 1071 Size: 612 96 363 Fragments arranged by size: 612 363 96 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 145 676 1071 Size: 145 531 395 Fragments arranged by size: 531 395 145 EcoO109I rG'GnC_Cy Cuts at: 0 732 1011 1071 Size: 732 279 60 Fragments arranged by size: 732 279 60 HaeII r_GCGC'y Cuts at: 0 140 1071 Size: 140 931 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 64 96 1071 Size: 64 32 975 Fragments arranged by size: 975 64 32 HincII GTy'rAC Cuts at: 0 645 1071 Size: 645 426 KasI G'GCGC_C Cuts at: 0 136 1071 Size: 136 935 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 950 1026 1071 Size: 950 76 45 Fragments arranged by size: 950 76 45 MscI TGG'CCA Cuts at: 0 508 936 1071 Size: 508 428 135 Fragments arranged by size: 508 428 135 MslI CAynn'nnrTG Cuts at: 0 22 667 1071 Size: 22 645 404 Fragments arranged by size: 645 404 22 MspA1I CmG'CkG Cuts at: 0 148 1043 1071 Size: 148 895 28 Fragments arranged by size: 895 148 28 NarI GG'CG_CC Cuts at: 0 137 1071 Size: 137 934 NcoI C'CATG_G Cuts at: 0 167 1071 Size: 167 904 NspI r_CATG'y Cuts at: 0 195 214 1071 Size: 195 19 857 Fragments arranged by size: 857 195 19 PfoI T'CCnGG_A Cuts at: 0 122 692 1071 Size: 122 570 379 Fragments arranged by size: 570 379 122 PpuMI rG'GwC_Cy Cuts at: 0 732 1071 Size: 732 339 PspXI vC'TCGA_Gb Cuts at: 0 196 1071 Size: 196 875 PstI C_TGCA'G Cuts at: 0 158 575 1071 Size: 158 417 496 Fragments arranged by size: 496 417 158 SacI G_AGCT'C Cuts at: 0 614 710 1071 Size: 614 96 361 Fragments arranged by size: 614 361 96 SacII CC_GC'GG Cuts at: 0 1044 1071 Size: 1044 27 SalI G'TCGA_C Cuts at: 0 643 1071 Size: 643 428 SfcI C'TryA_G Cuts at: 0 154 571 1071 Size: 154 417 500 Fragments arranged by size: 500 417 154 SfoI GGC'GCC Cuts at: 0 138 1071 Size: 138 933 SmaI CCC'GGG Cuts at: 0 829 1071 Size: 829 242 SmlI C'TyrA_G Cuts at: 0 196 391 709 1071 Size: 196 195 318 362 Fragments arranged by size: 362 318 196 195 SphI G_CATG'C Cuts at: 0 214 1071 Size: 214 857 SspI AAT'ATT Cuts at: 0 474 1071 Size: 474 597 StuI AGG'CCT Cuts at: 0 304 1071 Size: 304 767 StyI C'CwwG_G Cuts at: 0 167 633 1071 Size: 167 466 438 Fragments arranged by size: 466 438 167 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 103 1071 Size: 103 968 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 354 991 1071 Size: 354 637 80 Fragments arranged by size: 637 354 80 XhoI C'TCGA_G Cuts at: 0 196 1071 Size: 196 875 XmaI C'CCGG_G Cuts at: 0 827 1071 Size: 827 244 Enzymes that do cut: AccI AflIII AgeI AlwNI ApaLI ApoI AvaI BaeI BanI BanII BbeI BbsI BglI BlpI Bme1580I BmgBI BpmI BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrFI BssSI BstEII BstXI BtgI BtsI DraI EaeI EciI EcoICRI Eco57MI EcoO109I HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I NarI NcoI NspI PfoI PpuMI PspXI PstI SacI SacII SalI SfcI SfoI SmaI SmlI SphI SspI StuI StyI TaqII XcmI XhoI XmaI Enzymes that do not cut: AarI AatII Acc65I AclI AcuI AfeI AflII AhdI AleI AlfI AloI ApaI AscI AseI AsiSI AvrII BamHI BbvCI BcgI BciVI BclI BfrBI BglII BmrI BmtI BplI Bpu10I BsaAI BsaBI BsiEI BsiWI BsmI BsmBI BspEI BsrDI BsrGI BssHII BstAPI BstBI BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraIII DrdI EagI EarI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI KpnI MfeI MluI NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PsrI PvuI PvuII RsrII SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SrfI SwaI TatI TstI Tth111I XbaI XmnI ZraI