################################## # NO CUT ENZYMES ( 159 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp718I AspEI AspHI AspI AvaI AviII AvrII BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BmyI BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmFI BsoBI Bsp1286I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BssHII Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoT14I EcoT22I FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HindIII HpaII KasI KpnI KspI MamI MluI MroI MspI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I StyI SwaI TaqI Tth111I TthHB8I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AlwNI 1058 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG AseI 183 ATTAAT AT^TAAT AsnI 183 ATTAAT AT^TAAT Asp700I 418 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaII 15 GGACC G^G[AT]CC BalI 477 TGGCCA TGG^CCA BlpI 1077 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1077 GCTCAGC GC^T[ACGT]AGC BsaJI 1062 CCCTGG C^C[ACGT][ACGT]GG BsgI 1067 GTGCAG GTGCAG(16/14) BsmBI 654 GAGACG CGTCTC(1/5) Bsp1407I 84 TGTACA T^GTACA BspMI 17 ACCTGC ACCTGC(4/8) BsrDI 640 GCAATG GCAATG(2/0) BsrGI 84 TGTACA T^GTACA BssSI 1168 CACGAG CACGAG(-5/-1) BstAPI 1085 GCAGACTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Cac8I 1261 GCTGGC GC[ACGT]^[ACGT]GC CelII 1077 GCTCAGC GC^T[ACGT]AGC Cfr13I 15 GGACC G^G[ACGT]CC EaeI 477 TGGCCA [CT]^GGCC[GA] EcoRI 422 GAATTC G^AATTC EcoRV 544 GATATC GAT^ATC FauI 343 GCGGG CCCGC(4/6) HincII 606 GTTAAC GT[CT]^[GA]AC HindII 606 GTTAAC GT[CT]^[GA]AC HpaI 606 GTTAAC GTT^AAC HphI 1150 GGTGA GGTGA(8/7) MaeII 751 ACGT A^CGT MfeI 41 CAATTG C^AATTG MluNI 477 TGGCCA TGG^CCA MscI 477 TGGCCA TGG^CCA MslI 955 CATAGCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 11 CAGCGG C[AC]G^C[GT]G MunI 41 CAATTG C^AATTG NspI 493 ACATGC [GA]CATG^[CT] PflMI 1057 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 656 GACGAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 183 ATTAAT AT^TAAT PstI 691 CTGCAG CTGCA^G Sau96I 15 GGACC G^G[ACGT]CC SmlI 883 CTTGAG C^T[CT][GA]AG SspBI 84 TGTACA T^GTACA SspI 1003 AATATT AAT^ATT Tsp45I 1151 GTGAC ^GT[GC]AC Van91I 1057 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 418 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AcsI 422 GAATTC [GA]^AATT[CT] 1137 GAATTT AlwI 628 GATCC GGATC(4/5) 1198 GGATC ApoI 422 GAATTC [GA]^AATT[CT] 1137 GAATTT BfaI 379 CTAG C^TAG 832 CTAG BsmI 740 GAATGC GAATGC(1/-1) 398 GCATTC BsrI 152 ACTGG ACTGG(1/-1) 450 CCAGT BstYI 627 AGATCC [GA]^GATC[CT] 1198 GGATCT DpnII 628 GATC ^GATC 1199 GATC EarI 96 GAAGAG CTCTTC(1/4) 990 GAAGAG HinfI 156 GAATC G^A[ACGT]TC 507 GATTC Ksp632I 96 GAAGAG CTCTTC(1/4) 990 GAAGAG MaeI 379 CTAG C^TAG 832 CTAG MaeIII 1151 GTGAC ^GT[ACGT]AC 1248 GTTAC MboI 628 GATC ^GATC 1199 GATC MflI 627 AGATCC [GA]^GATC[CT] 1198 GGATCT NdeII 628 GATC ^GATC 1199 GATC Sau3AI 628 GATC ^GATC 1199 GATC SfcI 581 CTACAG C^T[GA][CT]AG 691 CTGCAG StuI 729 AGGCCT AGG^CCT 1097 AGGCCT TfiI 156 GAATC G^A[AT]TC 507 GATTC TspRI 511 CACTG CA[GC]TG[ACGT][ACGT]^ 636 CAGTG XhoII 627 AGATCC [GA]^GATC[CT] 1198 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 30 enzymes) # ######################################### AciI AfaI AluI BbvI BpmI BseRI BsiYI BslI BsmAI BssKI BstF5I BstNI DdeI EcoRII Fnu4HI FokI HaeIII ItaI MboII MnlI MseI MvaI MwoI NlaIII RsaI ScrFI SfaNI Tru9I TseI Tsp509I