(Circular) (Six-base) MAPSORT of: pFN21AE1450.seq Check: 7363 from: 1 to: 5989 pFN21AE1450 5989 bp With 182 enzymes: SgfI * August 16, 2010 14:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3799 278 Size: 53 83 186 936 2263 2468 Fragments arranged by size: 2468 2263 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3332 1937 Size: 1395 4594 Acc65I G'GTAC_C Cuts at: 3310 3310 Size: 5989 AclI AA'CG_TT Cuts at: 1563 4116 4489 1563 Size: 2553 373 3063 Fragments arranged by size: 3063 2553 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2917 4052 5436 5704 1632 Size: 1285 1135 1384 268 1917 Fragments arranged by size: 1917 1384 1285 1135 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5792 AflIII A'CryG_T Cuts at: 1203 4888 5865 1203 Size: 3685 977 1327 Fragments arranged by size: 3685 1327 977 AgeI A'CCGG_T Cuts at: 4820 4820 Size: 5989 AhdI GACnn_n'nnGTC Cuts at: 4718 4718 Size: 5989 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5989 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5989 AlwNI CAG_nnn'CTG Cuts at: 3068 3732 5304 3068 Size: 664 1572 3753 Fragments arranged by size: 3753 1572 664 ApaLI G'TGCA_C Cuts at: 4046 5202 4046 Size: 1156 4833 ApoI r'AATT_y Cuts at: 1493 2427 3142 3298 3524 3676 5785 1493 Size: 934 715 156 226 152 2109 1697 Fragments arranged by size: 2109 1697 934 715 226 156 152 AseI AT'TA_AT Cuts at: 160 1032 2189 4543 160 Size: 872 1157 2354 1606 Fragments arranged by size: 2354 1606 1157 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5989 AvaI C'yCGr_G Cuts at: 1870 1943 3314 1870 Size: 73 1371 4545 Fragments arranged by size: 4545 1371 73 BamHI G'GATC_C Cuts at: 3319 3686 3319 Size: 367 5622 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3310 4765 618 Size: 325 217 627 1523 1455 1842 Fragments arranged by size: 1842 1523 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3308 1391 Size: 1917 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5018 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4180 4214 1903 Size: 34 2243 34 3678 Fragments arranged by size: 3678 2243 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3881 5091 3881 Size: 1210 4779 BclI T'GATC_A Cuts at: 1548 1548 Size: 5989 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4600 136 Size: 107 122 71 631 605 2928 1525 Fragments arranged by size: 2928 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5984 5984 Size: 5989 BlpI GC'TnA_GC Cuts at: 3083 3406 3083 Size: 323 5666 Bme1580I G_kGCm'C Cuts at: 1790 4050 5206 1790 Size: 2260 1156 2573 Fragments arranged by size: 2573 2260 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5989 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3720 4678 442 Size: 416 53 83 399 24 2303 958 1753 Fragments arranged by size: 2303 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5989 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2429 2435 2746 3045 4649 1239 Size: 1190 6 311 299 1604 2579 Fragments arranged by size: 2579 1604 1190 311 299 6 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2909 4114 4979 5277 2909 Size: 1205 865 298 3621 Fragments arranged by size: 3621 1205 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4652 882 Size: 617 3153 2219 Fragments arranged by size: 3153 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3780 493 Size: 711 2576 2702 Fragments arranged by size: 2702 2576 711 BsaBI GATnn'nnATC Cuts at: 5983 5983 Size: 5989 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3796 4178 4813 275 Size: 53 83 186 936 2263 382 635 1451 Fragments arranged by size: 2263 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4422 4820 5094 5241 5652 4422 Size: 398 274 147 411 4759 Fragments arranged by size: 4759 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5764 Fragments arranged by size: 5764 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2267 2761 3263 1186 Size: 1081 494 502 3912 Fragments arranged by size: 3912 1081 502 494 BseYI C'CCAG_C Cuts at: 2906 3078 5192 5688 2906 Size: 172 2114 496 3207 Fragments arranged by size: 3207 2114 496 172 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3093 1163 Size: 573 1357 4059 Fragments arranged by size: 4059 1357 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3439 4200 5228 1540 Size: 275 186 1438 761 1028 2301 Fragments arranged by size: 2301 1438 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4050 4135 5206 4050 Size: 85 1071 4833 Fragments arranged by size: 4833 1071 85 BsmI GAATG_Cn' Cuts at: 2403 2751 3500 3593 2403 Size: 348 749 93 4799 Fragments arranged by size: 4799 749 348 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2653 2653 Size: 5989 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3308 4050 4135 5206 1391 Size: 399 1518 742 85 1071 2174 Fragments arranged by size: 2174 1518 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5652 5652 Size: 5989 BspHI T'CATG_A Cuts at: 3878 5608 3878 Size: 1730 4259 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2031 3344 844 Size: 1187 1313 3489 Fragments arranged by size: 3489 1313 1187 BsrBI CCG'CTC Cuts at: 1394 1823 3876 1394 Size: 429 2053 3507 Fragments arranged by size: 3507 2053 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2652 4484 4658 65 Size: 1183 1404 1832 174 1396 Fragments arranged by size: 1832 1404 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4633 4820 1700 Size: 110 2823 187 2869 Fragments arranged by size: 2869 2823 187 110 BsrGI T'GTAC_A Cuts at: 96 2089 96 Size: 1993 3996 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5989 BssSI C'ACGA_G Cuts at: 3173 4049 5061 3173 Size: 876 1012 4101 Fragments arranged by size: 4101 1012 876 BstAPI GCAn_nnn'nTGC Cuts at: 794 3096 794 Size: 2302 3687 BstBI TT'CG_AA Cuts at: 4793 4793 Size: 5989 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5989 BstYI r'GATC_y Cuts at: 1972 2632 3203 3319 3686 4071 4088 5540 5626 Size: 660 571 116 367 385 17 1452 86 Cuts at: 5626 5638 5984 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 660 571 385 367 346 116 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5437 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5250 Fragments arranged by size: 5250 683 56 BtsI GCAGTG_nn' Cuts at: 3505 4299 4319 3505 Size: 794 20 5175 Fragments arranged by size: 5175 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5989 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5954 DraI TTT'AAA Cuts at: 3293 3293 Size: 5989 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4996 809 Size: 507 3680 1802 Fragments arranged by size: 3680 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2482 3436 4325 Size: 54 1425 50 275 18 652 954 889 Cuts at: 4325 5954 8 Size: 1629 43 Fragments arranged by size: 1629 1425 954 889 652 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3436 1537 Size: 275 1624 4090 Fragments arranged by size: 4090 1624 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2096 2990 3919 1353 Size: 743 894 929 3423 Fragments arranged by size: 3423 929 894 743 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4576 4950 5096 387 Size: 1064 138 2987 374 146 1280 Fragments arranged by size: 2987 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2429 2435 2746 2917 3045 4052 4649 Size: 393 797 6 311 171 128 1007 597 Cuts at: 4649 5436 5704 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1007 797 787 597 393 311 268 171 128 6 EcoRI G'AATT_C Cuts at: 2427 3298 3676 2427 Size: 871 378 4740 Fragments arranged by size: 4740 871 378 EcoRV GAT'ATC Cuts at: 2551 2551 Size: 5989 FspI TGC'GCA Cuts at: 4495 4495 Size: 5989 HaeII r_GCGC'y Cuts at: 1678 5136 1678 Size: 3458 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2125 2157 4630 4662 4704 Size: 32 135 32 553 32 2473 32 42 Cuts at: 4704 4736 1373 Size: 32 2626 Fragments arranged by size: 2626 2473 553 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2613 3333 3579 669 Size: 988 281 675 720 246 3079 Fragments arranged by size: 3079 988 720 675 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5989 HpaI GTT'AAC Cuts at: 2613 3579 2613 Size: 966 5023 KpnI G_GTAC'C Cuts at: 3314 3314 Size: 5989 MfeI C'AATT_G Cuts at: 2046 3588 2046 Size: 1542 4447 MluI A'CGCG_T Cuts at: 5865 5865 Size: 5989 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2289 5103 5287 1305 Size: 984 2814 184 2007 Fragments arranged by size: 2814 2007 984 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2484 10 Size: 54 1425 343 652 3515 Fragments arranged by size: 3515 1425 652 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2964 3947 4306 4465 518 Size: 648 1798 983 359 159 2042 Fragments arranged by size: 2042 1798 983 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2018 3405 4082 5230 5475 1606 Size: 412 1387 677 1148 245 2120 Fragments arranged by size: 2120 1387 1148 677 412 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5437 NdeI CA'TA_TG Cuts at: 387 387 Size: 5989 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5989 NotI GC'GGCC_GC Cuts at: 3436 3436 Size: 5989 NspI r_CATG'y Cuts at: 1132 1229 2502 3347 4892 1132 Size: 97 1273 845 1545 2229 Fragments arranged by size: 2229 1545 1273 845 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5989 PciI A'CATG_T Cuts at: 4888 4888 Size: 5989 PflMI CCAn_nnn'nTGG Cuts at: 1108 3068 1108 Size: 1960 4029 PmeI GTTT'AAAC Cuts at: 3293 3293 Size: 5989 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4058 4090 5571 5603 4058 Size: 32 1481 32 4444 Fragments arranged by size: 4444 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2665 1613 Size: 1052 4937 PsiI TTA'TAA Cuts at: 3559 3559 Size: 5989 PstI C_TGCA'G Cuts at: 830 2700 3341 830 Size: 1870 641 3478 Fragments arranged by size: 3478 1870 641 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5989 SalI G'TCGA_C Cuts at: 1936 3331 1936 Size: 1395 4594 SbfI CC_TGCA'GG Cuts at: 3341 3341 Size: 5989 ScaI AGT'ACT Cuts at: 4237 5949 4237 Size: 1712 4277 SfcI C'TryA_G Cuts at: 826 1045 2586 2696 3337 4472 5153 5344 826 Size: 219 1541 110 641 1135 681 191 1471 Fragments arranged by size: 1541 1471 1135 681 641 219 191 110 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5989 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5989 SmaI CCC'GGG Cuts at: 1872 3316 1872 Size: 1444 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2888 4093 4994 5256 820 Size: 197 926 945 1205 901 262 1553 Fragments arranged by size: 1553 1205 945 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3780 493 Size: 3287 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5989 SphI G_CATG'C Cuts at: 1132 3347 1132 Size: 2215 3774 SspI AAT'ATT Cuts at: 5 52 3010 3913 5 Size: 47 2958 903 2081 Fragments arranged by size: 2958 2081 903 47 StuI AGG'CCT Cuts at: 1517 2736 3104 1517 Size: 1219 368 4402 Fragments arranged by size: 4402 1219 368 StyI C'CwwG_G Cuts at: 513 1065 3428 513 Size: 552 2363 3074 Fragments arranged by size: 3074 2363 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4016 4033 4186 4371 4816 325 Size: 1255 2436 17 153 185 445 1498 Fragments arranged by size: 2436 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2089 2457 4235 Size: 274 80 33 51 1443 112 368 1778 Cuts at: 4235 5947 96 Size: 1712 138 Fragments arranged by size: 1778 1712 1443 368 274 138 112 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5949 5981 5949 Size: 32 5957 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5989 XbaI T'CTAG_A Cuts at: 3325 3325 Size: 5989 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5989 XmaI C'CCGG_G Cuts at: 1870 3314 1870 Size: 1444 4545 XmnI GAAnn'nnTTC Cuts at: 2427 4118 2427 Size: 1691 4298 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3797 276 Size: 53 83 186 936 2263 2468 Fragments arranged by size: 2468 2263 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI