Restriction map for
pFN21AE0666
(Circular) (Six-base) MAPSORT of: pFN21AE0666.seq Check: 9559 from: 1 to: 5776
pFN21AE0666 5776 bp
With 182 enzymes: SgfI *
April 15, 2010 09:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3586 278
Size: 53 83 186 936 2050 2468
Fragments arranged by size:
2468 2050 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3119 1937
Size: 1182 4594
Acc65I G'GTAC_C
Cuts at: 3097 3097
Size: 5776
AclI AA'CG_TT
Cuts at: 1563 3903 4276 1563
Size: 2340 373 3063
Fragments arranged by size:
3063 2340 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3839 5223 5491 1632
Size: 2207 1384 268 1917
Fragments arranged by size:
2207 1917 1384 268
AfeI AGC'GCT
Cuts at: 2138 2138
Size: 5776
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5579
AflIII A'CryG_T
Cuts at: 1203 4675 5652 1203
Size: 3472 977 1327
Fragments arranged by size:
3472 1327 977
AgeI A'CCGG_T
Cuts at: 4607 4607
Size: 5776
AhdI GACnn_n'nnGTC
Cuts at: 2577 4505 2577
Size: 1928 3848
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5776
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5776
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2066 2098 2066
Size: 32 5744
AlwNI CAG_nnn'CTG
Cuts at: 3519 5091 3519
Size: 1572 4204
ApaLI G'TGCA_C
Cuts at: 3833 4989 3833
Size: 1156 4620
ApoI r'AATT_y
Cuts at: 1493 2711 3085 3311 3463 5572 1493
Size: 1218 374 226 152 2109 1697
Fragments arranged by size:
2109 1697 1218 374 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2846 4330 160
Size: 872 1814 1484 1606
Fragments arranged by size:
1814 1606 1484 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5776
AvaI C'yCGr_G
Cuts at: 1870 1943 3101 1870
Size: 73 1158 4545
Fragments arranged by size:
4545 1158 73
BamHI G'GATC_C
Cuts at: 3106 3473 3106
Size: 367 5409
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2032 2312 3097 4552 618
Size: 325 217 627 245 280 785 1455 1842
Fragments arranged by size:
1842 1455 785 627 325 280 245 217
BanII G_rGCy'C
Cuts at: 1391 2220 3095 1391
Size: 829 875 4072
Fragments arranged by size:
4072 875 829
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2129 2221 928
Size: 971 230 92 4483
Fragments arranged by size:
4483 971 230 92
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3967 4001 1903
Size: 34 2030 34 3678
Fragments arranged by size:
3678 2030 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3668 4878 3668
Size: 1210 4566
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5776
BfrBI ATG'CAT
Cuts at: 2723 2723
Size: 5776
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4387 136
Size: 107 122 71 631 605 2715 1525
Fragments arranged by size:
2715 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2863 5771 2863
Size: 2908 2868
BlpI GC'TnA_GC
Cuts at: 3193 3193
Size: 5776
Bme1580I G_kGCm'C
Cuts at: 1790 3837 4993 1790
Size: 2047 1156 2573
Fragments arranged by size:
2573 2047 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5776
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3507 4465 442
Size: 416 53 83 399 24 2090 958 1753
Fragments arranged by size:
2090 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5776
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2349 2349
Size: 5776
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2037 2373 2828 4436 1239
Size: 798 336 455 1608 2579
Fragments arranged by size:
2579 1608 798 455 336
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2093 2306 3901 4766 5064 2093
Size: 213 1595 865 298 2805
Fragments arranged by size:
2805 1595 865 298 213
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4439 882
Size: 617 2940 2219
Fragments arranged by size:
2940 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3567 493
Size: 711 2363 2702
Fragments arranged by size:
2702 2363 711
BsaBI GATnn'nnATC
Cuts at: 5770 5770
Size: 5776
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2875 3583 3965 4600
Size: 53 83 186 936 1342 708 382 635
Cuts at: 4600 275
Size: 1451
Fragments arranged by size:
1451 1342 936 708 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4209 4607 4881 5028 5439 4209
Size: 398 274 147 411 4546
Fragments arranged by size:
4546 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2066 2096 1178
Size: 30 165 30 663 30 4858
Fragments arranged by size:
4858 663 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2087 2195 2972 1186
Size: 901 108 777 3990
Fragments arranged by size:
3990 901 777 108
BseYI C'CCAG_C
Cuts at: 2219 4979 5475 2219
Size: 2760 496 2520
Fragments arranged by size:
2760 2520 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2505 1163
Size: 573 769 4434
Fragments arranged by size:
4434 769 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3226 3987 5015 1540
Size: 275 186 1225 761 1028 2301
Fragments arranged by size:
2301 1225 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3837 3922 4993 3837
Size: 85 1071 4620
Fragments arranged by size:
4620 1071 85
BsmI GAATG_Cn'
Cuts at: 2555 3287 3380 2555
Size: 732 93 4951
Fragments arranged by size:
4951 732 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2220 3095 3837 3922 4993 1391
Size: 399 430 875 742 85 1071 2174
Fragments arranged by size:
2174 1071 875 742 430 399 85
BspEI T'CCGG_A
Cuts at: 5439 5439
Size: 5776
BspHI T'CATG_A
Cuts at: 3665 5395 3665
Size: 1730 4046
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2761 3131 844
Size: 1917 370 3489
Fragments arranged by size:
3489 1917 370
BsrBI CCG'CTC
Cuts at: 1394 1823 3663 1394
Size: 429 1840 3507
Fragments arranged by size:
3507 1840 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4271 4445 65
Size: 1183 3023 174 1396
Fragments arranged by size:
3023 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2029 4420 4607 1700
Size: 110 219 2391 187 2869
Fragments arranged by size:
2869 2391 219 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5776
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5776
BssSI C'ACGA_G
Cuts at: 3836 4848 3836
Size: 1012 4764
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5776
BstBI TT'CG_AA
Cuts at: 2202 4580 2202
Size: 2378 3398
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5776
BstXI CCAn_nnnn'nTGG
Cuts at: 2281 2281
Size: 5776
BstYI r'GATC_y
Cuts at: 1972 2863 3106 3473 3858 3875 5327 5413 5425
Size: 891 243 367 385 17 1452 86 12
Cuts at: 5425 5771 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 891 385 367 346 243 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2245 2598 513
Size: 552 939 241 353 3691
Fragments arranged by size:
3691 939 552 353 241
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5037
Fragments arranged by size:
5037 683 56
BtsI GCAGTG_nn'
Cuts at: 3292 4086 4106 3292
Size: 794 20 4962
Fragments arranged by size:
4962 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5776
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5741
DraI TTT'AAA
Cuts at: 3080 3080
Size: 5776
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4783 809
Size: 507 3467 1802
Fragments arranged by size:
3467 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3223 4112 5741
Size: 54 1425 50 275 18 1393 889 1629
Cuts at: 5741 8
Size: 43
Fragments arranged by size:
1629 1425 1393 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3223 1537
Size: 275 1411 4090
Fragments arranged by size:
4090 1411 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2153 2204 2949 3706 1353
Size: 800 51 745 757 3423
Fragments arranged by size:
3423 800 757 745 51
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4363 4737 4883 387
Size: 1064 138 2774 374 146 1280
Fragments arranged by size:
2774 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2037 2373 2828 3839 4436 5223 5491
Size: 393 405 336 455 1011 597 787 268
Cuts at: 5491 1239
Size: 1524
Fragments arranged by size:
1524 1011 787 597 455 405 393 336
268
EcoRI G'AATT_C
Cuts at: 3085 3463 3085
Size: 378 5398
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2215 2215
Size: 5776
FspI TGC'GCA
Cuts at: 4282 4282
Size: 5776
HaeII r_GCGC'y
Cuts at: 1678 2012 2140 4923 1678
Size: 334 128 2783 2531
Fragments arranged by size:
2783 2531 334 128
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2563 2595 2708 2740 2848
Size: 32 135 32 991 32 113 32 108
Cuts at: 2848 2880 4417 4449 4491 4523 1373
Size: 32 1537 32 42 32 2626
Fragments arranged by size:
2626 1537 991 135 113 108 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3120 3366 669
Size: 988 281 1182 246 3079
Fragments arranged by size:
3079 1182 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5776
HpaI GTT'AAC
Cuts at: 3366 3366
Size: 5776
KpnI G_GTAC'C
Cuts at: 3101 3101
Size: 5776
MfeI C'AATT_G
Cuts at: 3375 3375
Size: 5776
MluI A'CGCG_T
Cuts at: 5652 5652
Size: 5776
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3013 4890 5074 1305
Size: 1708 1877 184 2007
Fragments arranged by size:
2007 1877 1708 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3954
Fragments arranged by size:
3954 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2728 3734 4093 4252 518
Size: 648 1562 1006 359 159 2042
Fragments arranged by size:
2042 1562 1006 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3192 3869 5017 5262 1606
Size: 1586 677 1148 245 2120
Fragments arranged by size:
2120 1586 1148 677 245
NaeI GCC'GGC
Cuts at: 2031 2031
Size: 5776
NcoI C'CATG_G
Cuts at: 513 1065 2004 2598 513
Size: 552 939 594 3691
Fragments arranged by size:
3691 939 594 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5776
NgoMIV G'CCGG_C
Cuts at: 2029 2029
Size: 5776
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5776
NotI GC'GGCC_GC
Cuts at: 3223 3223
Size: 5776
NsiI A_TGCA'T
Cuts at: 2725 2725
Size: 5776
NspI r_CATG'y
Cuts at: 1132 1229 3134 4679 1132
Size: 97 1905 1545 2229
Fragments arranged by size:
2229 1905 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5776
PciI A'CATG_T
Cuts at: 4675 4675
Size: 5776
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5776
PmeI GTTT'AAAC
Cuts at: 3080 3080
Size: 5776
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2066 2098 3845 3877 5358 5390 2066
Size: 32 1747 32 1481 32 2452
Fragments arranged by size:
2452 1747 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5776
PsiI TTA'TAA
Cuts at: 3346 3346
Size: 5776
PstI C_TGCA'G
Cuts at: 830 3128 830
Size: 2298 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5776
SalI G'TCGA_C
Cuts at: 1936 3118 1936
Size: 1182 4594
SapI GCTCTTCn'nnn_
Cuts at: 2204 2204
Size: 5776
SbfI CC_TGCA'GG
Cuts at: 3128 3128
Size: 5776
ScaI AGT'ACT
Cuts at: 4024 5736 4024
Size: 1712 4064
SfcI C'TryA_G
Cuts at: 826 1045 2481 3124 4259 4940 5131 826
Size: 219 1436 643 1135 681 191 1471
Fragments arranged by size:
1471 1436 1135 681 643 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5776
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5776
SmaI CCC'GGG
Cuts at: 1872 3103 1872
Size: 1231 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2108 2321 3880 4781 5043 820
Size: 197 926 165 213 1559 901 262 1553
Fragments arranged by size:
1559 1553 926 901 262 213 197 165
SnaBI TAC'GTA
Cuts at: 493 3567 493
Size: 3074 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5776
SphI G_CATG'C
Cuts at: 1132 3134 1132
Size: 2002 3774
SspI AAT'ATT
Cuts at: 5 52 3700 5
Size: 47 3648 2081
Fragments arranged by size:
3648 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5776
StyI C'CwwG_G
Cuts at: 513 1065 2004 2598 3215 513
Size: 552 939 594 617 3074
Fragments arranged by size:
3074 939 617 594 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3803 3820 3973 4158 4603 325
Size: 1255 2223 17 153 185 445 1498
Fragments arranged by size:
2223 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4022 5734 96
Size: 274 80 33 51 1443 2045 1712 138
Fragments arranged by size:
2045 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5736 5768 5736
Size: 32 5744
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5776
XbaI T'CTAG_A
Cuts at: 3112 3112
Size: 5776
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2803 2803
Size: 5776
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5776
XmaI C'CCGG_G
Cuts at: 1870 3101 1870
Size: 1231 4545
XmnI GAAnn'nnTTC
Cuts at: 3905 3905
Size: 5776
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3584 276
Size: 53 83 186 936 2050 2468
Fragments arranged by size:
2468 2050 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NsiI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I
EcoRV FseI FspAI KasI NarI NruI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SexAI SfiI SfoI SrfI SwaI